Article
Plant Sciences
Shuiling Ji, Bernhard Grimm, Peng Wang
Summary: This study reveals the distinct roles of cpSRP43 and cpSRP54 in optimizing the function of the major PORB isoform in Arabidopsis, with the former stabilizing the enzyme and providing appropriate amounts of PORB and the latter enhancing its binding to the thylakoid membrane to ensure adequate metabolic flux.
Article
Biophysics
Dustin R. Baucom, Mercede Furr, Vivek Govind Kumar, Patience Okoto, James L. Losey, Ralph L. Henry, Mahmoud Moradi, Thallapuranam Krishnaswamy Suresh Kumar, Colin D. Heyes
Summary: The study reveals that Alb3-Cterm contains transient secondary structure in certain motifs, which may play a crucial role in its recognition and binding to the transit complex. The excellent agreement between experimental and computational data allowed for the successful prediction of the apparent unfolding free energy of this secondary structure.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ying-Qi Wang, Jing-Jing Ye, Hong-Zhiyuan Yang, Da Li, Xiao-Xiang Li, Yong-Kang Wang, Xin-Qiang Zheng, Jian-Hui Ye, Qing-Sheng Li, Yue-Rong Liang, Jian-Liang Lu
Summary: The light-sensitive albino tea plant can gradually recover its photosynthetic pigments, chloroplast ultrastructure, and photosynthesis parameters from pale yellow to green through short-term shading treatment. This is attributed to the proper formation of pigment-protein complexes on the thylakoid membrane, benefiting from increased levels of LHCII subunits, particularly the deficiency of Lhcb1, in the shaded leaves of 'HJY'.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Eugene Bukhanov, Alexandr Shabanov, Mikhail N. Volochaev, Svetlana A. Pyatina
Summary: The propagation of light in plant leaves is influenced by the ordering in chloroplasts and spectral characteristics of pigments. This study demonstrates a method for determining the hidden ordered structure, revealing a band gap in the visible spectrum and an increase in photon states density. The spectrum shifts towards the wavelength range of effective photosynthesis due to the effective refractive index and dispersion in the chlorophyll absorption spectrum area.
Review
Biochemistry & Molecular Biology
Martin R. Pool
Summary: The endoplasmic reticulum serves as the entry point for the secretory pathway, responsible for targeting proteins to their appropriate destinations. This targeting process involves the recognition of specific targeting signals and their subsequent delivery to the translocases present at the ER membrane. Targeting is typically mediated by cleavable N-terminal hydrophobic signal sequences or internal membrane anchor sequences, and the location and features of the targeting sequence determine which targeting pathway substrates are used.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Oliver Weichenrieder
Summary: In this study, Juaire et al. investigated disease-associated variants of the SRP54 GTPase using X-ray crystallography, biophysical tools, and cell-based assays. They demonstrated that defects in SRP-mediated protein secretion can explain phenotypes of severe neutropenia with Shwachman-Diamond-syndrome-like symptoms.
Article
Plant Sciences
Lulu Ren, Hongping Ma, Donghui Chao, Haizhen Zhang, Kun Qiao, Shuang Feng, Aimin Zhou
Summary: In this study, the functions of two Arabidopsis (Arabidopsis thaliana) Sep proteins, Sep1 and Sep2, in Chl breakdown and in response to light stress were investigated and compared. It was found that Sep2 reduced the levels of Chl by promoting Chl breakdown and could alleviate the accumulation of reactive oxygen species under long-term light stress. However, Sep1 did not cause Chl breakdown in Arabidopsis leaves. These findings suggest that Sep2, as a member of the LHC-like family, might regulate the levels of free Chl by binding to Chl and participate in light-stress response.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Multidisciplinary
Yijia Li, Chunlei Xia, Ruizhen Tian, Linlu Zhao, Jinxing Hou, Jieqiong Wang, Quan Luo, Jiayun Xu, Liang Wang, Chunxi Hou, Bai Yang, Hongcheng Sun, Junqiu Liu
Summary: A controllable protein nanostructures-based On/Off switchable artificial light-harvesting system with sequential multistep energy transfer and photocatalysis was reported, mimicking the natural system in structure and function. Single-layered protein nanosheets were constructed with an ordered arrangement of pigments, and fluorescent chromophores were distributed on the nanosheets via electrostatic interactions. The system achieved sequential energy transfer and high-efficiency photocatalysis. The catalytic process could be regulated by an On/Off switch.
Article
Plant Sciences
Tingting Zhang, Xiangyu Dong, Xin Yuan, Yuanyuan Hong, Lingling Zhang, Xuan Zhang, Shuxia Chen
Summary: This study identified the candidate gene CsSRP43 in a spontaneous cucumber mutant, revealing its crucial role in leaf color and photosynthesis pathways. Through gene cloning and sequence analysis, the study provided insights into the molecular mechanisms underlying leaf color formation in cucumber.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Mithun Kumar Rathod, Sreedhar Nellaepalli, Shin-Ichiro Ozawa, Hiroshi Kuroda, Natsumi Kodama, Sandrine Bujaldon, Francis-Andre Wollman, Yuichiro Takahashi
Summary: Chlamydomonas reinhardtii's LHCs play a vital role in the function of photosystems. The protein Alb3.1 plays a key role in the assembly of LHCs, interacting with cpSRP43 and cpSRP54 proteins to form an assembly apparatus for most LHCs in the thylakoid membranes.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Masaharu Kondo, Haruka Matsuda, Tomoyasu Noji, Mamoru Nango, Takehisa Dewa
Summary: The study found that the monomeric form of LHCII has higher photocatalytic activity compared to the trimer form, as well as more significant fluorescence quenching by methylviologen. In the presence of Pt nanoparticles in a catalytic solution system, photoinduced hydrogen production was observed for the LHCII monomer.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Review
Cell Biology
Xiumei Xu, Min Ouyang, Dandan Lu, Canhui Zheng, Lixin Zhang
Summary: Chloroplasts have multiple suborganellar membranes, and the correct and efficient translocation of chloroplast proteins is fundamental. Recent studies have explored new components and regulatory mechanisms involved in chloroplast protein import and sorting, including the novel mechanism of liquid-liquid phase transition (LLPT) for regulating chloroplast protein sorting. Overall, LLPT may be a universal and conserved mechanism for organelle protein trafficking and biogenesis.
TRENDS IN CELL BIOLOGY
(2021)
Article
Chemistry, Physical
Ashley M. Hancock, Minjung Son, Muath Nairat, Tiejun Wei, Lars J. C. Jeuken, Christopher D. P. Duffy, Gabriela S. Schlau-Cohen, Peter G. Adams
Summary: Light-Harvesting Complex II (LHCII) is a membrane protein that plays a crucial role in absorbing solar energy and transferring it to Photosystem II. A recent study enhanced absorption in the green spectral region by organizing TR and LHCII into a lipid nanodisc. Fluorescence spectroscopy showed a 60% efficiency in energy transfer and a 262% enhancement of LHCII fluorescence in the 525-625 nm range, with ultrafast transient absorption spectroscopy revealing two time constants for energy transfer. Structural modeling and theoretical calculations indicated different separations for TR-to-LHCII interactions, showcasing the potential of the nanodisc-based biohybrid system in exploring photophysical interactions between chromophores and membrane proteins.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Biotechnology & Applied Microbiology
Jiri Masojidek, Karolina Ranglova, Martina Beckova, Giuseppe Torzillo, Jana Knoppova, Ana Margarita Silva Benavides, Filip Charvat, Josef Komenda
Summary: The photosynthetic performance and electron transport rate of Chlorella cultures with reduced antenna size were studied during outdoor trials. The results showed that the strain with reduced antenna size had lower oxygen production and electron transport rate, but higher non-photochemical quenching and respiration compared to the strain with full antenna size. These findings suggest that the use of microalgae strains with reduced antenna size for outdoor mass cultivation may not be as effective as expected.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Multidisciplinary Sciences
Christopher Gorski, Yuval Mazor
Summary: This protocol describes a method to isolate and study Photosystem I (PSI) and its antenna complex, Light Harvesting Complex I (LHCI), from plants. PSI-LHCI is a large membrane protein complex that plays a crucial role in light harvesting and electron transport in photosynthesis. The protocol involves homogenization of plant tissue, separation of chloroplasts, lysis of chloroplasts to recover thylakoid membranes, solubilization of thylakoid membranes, and purification of chlorophyll-containing complexes using anion exchange and sucrose gradient centrifugation. The resulting fractions are characterized to identify and study the PSI-LHCI complex. This protocol is based on previous methods developed in the lab of Nathan Nelson and provides a simplified approach for isolating and studying PSI-LHCI.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Bjoern Walter, Athina Hristou, Marc M. Nowaczyk, Danja Schuenemann
BIOCHEMICAL JOURNAL
(2015)
Article
Biochemistry & Molecular Biology
Beatrix Duenschede, Chantal Traeger, Christine Vera Schoeder, Dominik Ziehe, Bjoern Walter, Silke Funke, Eckhard Hofmann, Danja Schuenemann
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Plant Sciences
Bjoern Walter, Thomas Pieta, Danja Schuenemann
Frontiers in Plant Science
(2015)
Review
Plant Sciences
Dominik Ziehe, Beatrix Duenschede, Danja Schuenemann
PHOTOSYNTHESIS RESEARCH
(2018)
Article
Plant Sciences
Anja Schneider, Iris Steinberger, Henning Strissel, Hans-Henning Kunz, Nikolay Manavski, Joerg Meurer, Gabi Burkhard, Sabine Jarzombski, Danja Schuenemann, Stefan Geimer, Ulf-Ingo Fluegge, Dario Leister
Article
Multidisciplinary Sciences
Anna Schoenberg, Elena Bergner, Stefan Helm, Birgit Agne, Beatrix Duenschede, Danja Schuenemann, Mike Schutkowski, Sacha Baginsky
Article
Multidisciplinary Sciences
Dominik Ziehe, Beatrix Diinschede, Mira Zenker, Silke Funke, Marc M. Nowaczyk, Danja Schunemann
Article
Multidisciplinary Sciences
Jose G. Garcia-Cerdan, Ariel L. Furst, Kent L. McDonald, Danja Schuenemann, Matthew B. Francis, Krishna K. Niyogi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Athina Hristou, Ines Gerlach, Dominique S. Stolle, Jennifer Neumann, Annika Bischoff, Beatrix Duenschede, Marc M. Nowaczyk, Reimo Zoschke, Danja Schuenemann
Article
Microbiology
Beate Sauerbrei, Jan Arends, Danja Schunemann, Franz Narberhaus
JOURNAL OF BACTERIOLOGY
(2020)
Article
Multidisciplinary Sciences
Thilo Ruehle, Marcel Dann, Bennet Reiter, Danja Schuenemann, Belen Naranjo, Jan-Ferdinand Penzler, Tatjana Kleine, Dario Leister
Summary: The study reveals that PGRL1 and PGRL2 modulate PGR5 activity and stability, affecting the mediation process of cyclic electron flow (CEF).
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Bernd Ackermann, Beatrix Duenschede, Bjoern Pietzenuk, Bo Hojen Justesen, Ute Kraemer, Eckhard Hofmann, Thomas Guenther Pomorski, Danja Schuenemann
Summary: Members of the Oxa1/YidC/Alb3 protein family are involved in the insertion, folding, and assembly of membrane proteins in mitochondria, bacteria, and chloroplasts. The thylakoid membrane protein Alb3 interacts with ribosomes during posttranslational insertion of specific proteins, while Alb4, a homolog located in the same membrane, does not show ribosome binding. Alb3 contacts ribosomes via its C-terminal region and at least one additional binding site within its hydrophobic core.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jakob-Maximilian Keller, Maureen Julia Frieboes, Ludwig Joedecke, Sandrine Kappel, Natalia Wulff, Tobias Rindfleisch, Omar Sandoval-Ibanez, Ines Gerlach, Wolfram Thiele, Ralph Bock, Juergen Eirich, Iris Finkemeier, Danja Schuenemann, Reimo Zoschke, Mark Aurel Schoettler, Ute Armbruster
Summary: This study demonstrates the role of the DECREASED ELECTRON TRANSPORT AT PSII (DEAP2) assembly factor in the early assembly of photosystem II (PSII) in plants. It shows that DEAP2 works together with the PHOTOSYNTHESIS AFFECTED MUTANT 68 (PAM68) factor to facilitate the formation of the RC47 intermediate. The loss of both DEAP2 and PAM68 leads to a loss of functional PSII and the inability of plants to grow photoautotrophically.
Review
Biochemistry & Molecular Biology
Dominik Ziehe, Beatrix Duenschede, Danja Schuenemann
BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Magnus Alm Rosenblad, Chantal Traeger, Danja Schuenemann
PLANT SIGNALING & BEHAVIOR
(2013)
