Article
Biochemistry & Molecular Biology
Qiuling Liu, Tujing Song, Bing Chen, Jingjing Zhang, Wen Li
Summary: Hypoxia is crucial for the development of various disorders, especially hypoxic cerebropathy. The damage to the blood-brain barrier (BBB) caused by hypoxia is well known. This study explored the correlation between endothelial ferroptosis and hypoxia-induced BBB damage using in vivo zebrafish and in vitro bEnd.3 cells. The results showed that hypoxic treatment could induce BBB disruption by down-regulating claudin-5 (CLDN5) expression in both zebrafish cerebrovascular endothelial cells and bEnd.3 cells.
Article
Immunology
Xiaolong He, Lei Wang, Liqun Liu, Jie Gao, Beiguo Long, Feng Chi, Tongtong Hu, Yu Wan, Zelong Gong, Li Li, Peilin Zhen, Tiesong Zhang, Hong Cao, Sheng-He Huang
Summary: E. coli K1 infection triggers the release of SLURP1, an endogenous ligand of alpha 7 nAChR, which is crucial for E. coli K1 invasion and neutrophil migration across the BBB. SLURP1 is also required for E. coli K1-induced alpha 7 nAChR activation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Rui-Cheng Yang, Kun Huang, Hui-Peng Zhang, Liang Li, Yu-Fei Zhang, Chen Tan, Huan-Chun Chen, Mei-Lin Jin, Xiang-Ru Wang
Summary: The study found that SARS-CoV-2 infection can cause dysfunction of the blood-brain barrier, increasing its permeability, which provides new insights into the neuropathogenesis of SARS-CoV-2.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Jiang Fang, Qiang Yuan, Zhuoying Du, Maoxing Fei, Quan Zhang, Lei Yang, Meihua Wang, Weijian Yang, Jian Yu, Gang Wu, Jin Hu
Summary: Ferroptosis plays an important role in traumatic brain injury (TBI) and is involved in the damage of brain microvascular endothelial cells and the blood-brain barrier. In this study, they found that ferroptosis-related markers were altered in brain endothelial cells after TBI both in vitro and in vivo. Additionally, inhibition of ferroptosis with Fer-1 reduced BMVECs death, BBB permeability, and tight junction loss, and exerted neuroprotective effects in the early stage of TBI.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Ronghua Zou, Xiaoni Wu, Qiwei Chen, Xiaowei Gong, Yuefeng Chu, Fuying Zheng
Summary: In this study, it was found that the OmpA protein of R. anatipestifer is an important virulence factor mediating its invasion of duck brain microvascular endothelial cells and penetration of the blood-brain barrier. The amino acids 230-242 of OmpA represent a key domain involved in R. anatipestifer invasion.
VETERINARY MICROBIOLOGY
(2023)
Article
Geriatrics & Gerontology
Alpna Tyagi, Carol Mirita, Iman Shah, P. Hemachandra Reddy, Subbiah Pugazhenthi
Summary: This study suggests that Sirt3 deficiency exacerbates the harmful effects of lipotoxicity on BMECs, leading to aggravated neuroinflammation.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Jin Zhang, Hongwu Sun, Chen Gao, Ying Wang, Xin Cheng, Yun Yang, Qiang Gou, Langhuang Lei, Yanping Chen, Xingyong Wang, Quanming Zou, Jiang Gu
Summary: A successful strategy to improve the stability of recombinant Vo using chitosan-modified PLGA nanoparticles was identified, maintaining its immunogenicity and demonstrating immunoprotection in a mouse model.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Qingliang Yang, Harshani Wijerathne, Jordan C. Langston, Mohammad F. Kiani, Laurie E. Kilpatrick
Summary: The endothelium, the inner layer of blood vessels, plays a crucial role in hemostasis and regulation of systemic inflammatory response. However, there are concerns about the phenotypic heterogeneity of microvascular endothelial cells between different organs and species, which may pose challenges in developing therapeutics targeting endothelium inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Tyler M. Lu, Sean Houghton, Tarig Magdeldin, Jose Gabriel Barcia Duran, Andrew P. Minotti, Amanda Snead, Andrew Sproul, Duc-Huy T. Nguyen, Jenny Xiang, Howard A. Fine, Zev Rosenwaks, Lorenz Studer, Shahin Rafii, Dritan Agalliu, David Redmond, Raphael Lis
Summary: Researchers have found that iBMECs lack functional attributes of ECs and instead express genes related to the neuroectodermal epithelial lineage. By overexpressing key endothelial ETS transcription factors, Epi-iBMECs can be reprogrammed into authentic endothelial cells that are congruent with bona fide endothelium at both transcriptomic and some functional levels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Xue-Wei Zhang, Ming-Xin An, Zeng-Kang Huang, Lan Ma, Dan Zhao, Zhao Yang, Jun-Xiu Shi, Dong-Xin Liu, Qiang Li, An-Hua Wu, Yu-Hua Chen, Wei-Dong Zhao
Summary: This study shows that deletion of the Lpp gene in E. coli reduces its pathogenicity in causing neonatal meningitis. The Lpp-deleted E. coli is susceptible to neutrophil bactericidal activity, with increased production of ROS and activation of NADPH oxidase. This enhanced survival is associated with the upregulation of FliC and suppression of NADPH oxidase activation in neutrophils.
Article
Chemistry, Multidisciplinary
Hinako Nagano, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: The study suggests that INSR-mediated signaling is involved in regulating the expression of functional proteins at the blood-brain barrier (BBB), contributing to BBB function maintenance. Changes in protein expressions, particularly those related to amyloid-beta peptides, in INSR knockdown cells may contribute to the development of cerebral amyloid angiopathy through the suppression of INSR-mediated signaling.
PHARMACEUTICAL RESEARCH
(2022)
Article
Cell Biology
Jing Zhao, Yan Wang, Dong Wang, Wei Yan, Shishuang Zhang, Dai Li, Zhaoli Han, Fanglian Chen, Ping Lei
Summary: This study investigated the impact of miR-124-3p on brain microvascular endothelial cell function and found that it can promote cell repair and protect against TBI-induced damage by inhibiting mTOR signaling and inducing autophagy.
HISTOLOGY AND HISTOPATHOLOGY
(2022)
Article
Chemistry, Medicinal
Hinako Nagano, Seiryo Ogata, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Summary: This study reveals the role of Podocalyxin (PODXL) in blood-brain barrier function. PODXL knockdown affects immune response and intracellular trafficking, and regulates the protein expression and efflux activity of ABCB1/MDR1.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Neurosciences
Guneet Kaur, Pallavi Pant, Reshma Bhagat, Pankaj Seth
Summary: In this study, a physiologically relevant blood-brain barrier (BBB) model was established using human brain microvascular endothelial cells (hBMECs) and astrocytes derived from human progenitors. The effects of Zika virus envelope (E) protein on the cells comprising the BBB were investigated using this model. The presence of ZIKV E protein led to decreased expression of crucial endothelial junction proteins, increased BBB permeability, and inflammatory response in astrocytes.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Debin Tian, Wen Li, C. Lynn Heffron, Bo Wang, Hassan M. Mahsoub, Harini Sooryanarain, Anna M. Hassebroek, Sherrie Clark-Deener, Tanya LeRoith, Xiang-Jin Meng
Summary: This study demonstrates that the hepatitis E virus (HEV) can cross the blood-brain barrier and both quasi-enveloped and nonenveloped HEVs have similar abilities in this process. Additionally, human brain microvascular endothelial cells are susceptible to HEV infection, and similar results were observed in pigs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)