期刊
FEBS LETTERS
卷 583, 期 14, 页码 2441-2445出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2009.05.042
关键词
Targeted mass spectrometry; Alternative translation initiation; Dok-1 protein isoform; N(alpha)-acetylation; N-terminal truncation
Alternative translation initiation is a mechanism whereby functionally altered proteins are produced from a single mRNA. Internal initiation of translation generates N-terminally truncated protein isoforms, but such isoforms observed in immunoblot analysis are often overlooked or dismissed as degradation products. We identified an N-terminally truncated isoform of human Dok-1 with N-terminal acetylation as seen in the wild-type. This Dok-1 isoform exhibited distinct perinuclear localization whereas the wild-type protein was distributed throughout the cytoplasm. Targeted analysis of blocked N-terminal peptides provides rapid identification of protein isoforms and could be widely applied for the general evaluation of perplexing immunoblot bands. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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