期刊
FEBS LETTERS
卷 583, 期 4, 页码 754-758出版社
WILEY
DOI: 10.1016/j.febslet.2009.01.027
关键词
Transient-receptor-potential channel; Mechanosensitivity; Thermosensitivity; TRPV4; TRPV1; Polyunsaturated fatty acid
资金
- NIH [GM047856]
- Vilas Trust of the University of Wisconsin, Madison
Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca2+ rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks activate TRPV4 but not TRPV1. Osmotic swelling is modeled to activate enzyme(s), producing polyunsaturated fatty acids (PUFAs) to open TRPV4 in mammalian cells. This model relegates mechanosensitivity to the enzyme and not the channel. Yeast has only a single Delta 9 fatty-acid monodesaturase and cannot make PUFAs suggesting an alternative mechanism for TRPV4 activation. We discuss possible explanations of this difference. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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