期刊
FEBS LETTERS
卷 583, 期 21, 页码 3525-3530出版社
WILEY
DOI: 10.1016/j.febslet.2009.10.016
关键词
Lipoic acid; Caspase; Nitric oxide; Dihydrolipoic; Denitrosation
资金
- NIH [GM044100]
- Walter Reed Army Institute of Research [W81XWH-09-P-0631, W81XWH-06-1-0247]
- Office of Naval Research [42237]
Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-L-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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