Article
Immunology
Xuesong Zhang, Yuansheng Duan, Jinru Ma, Zhibing Liu, Yani Ding, Haibo Xie, Jiale Peng, Feng Wang, Jiaxiang Zhang, Qixing Zhu
Summary: Trichloroethylene (TCE), a widely used volatile chlorinated solvent, can cause adverse effects on human and animal health. This study investigates the role of Sirtuin-1 (SIRT 1) in TCE-induced immune renal tubular injury. The results suggest that SIRT 1 alleviates renal tubular epithelial cells immune injury by inhibiting the release of HSP 70 and weakening its interaction with TLR 4.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Javad Mahmoudi, Leila Hosseini, Saeed Sadigh-Eteghad, Fereshteh Farajdokht, Seyed Mehdi Vatandoust, Mojtaba Ziaee
Summary: The study showed that sericin administration at higher doses can reverse anxiety-like behavior and cognitive deficits induced by heat stress. Sericin may exert its protective effects against heat stress-mediated cognitive dysfunction and anxiety-like behavior possibly through suppressing HSP-70, oxidative stress, and apoptosis.
NEUROCHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Qin Yao, Patricia Outeda, Hangxue Xu, Rebecca Walker, Denis Basquin, Feng Qian, Liudmila Cebotaru, Terry Watnick, Valeriu Cebotaru
Summary: Autosomal dominant polycystic kidney disease is a common inherited renal disorder caused by mutations in PKD1 or PKD2 genes. Increased expression of PC2 in PKD1 mutant tissues is due in part to an increase in Pkd2 mRNA, as well as more effective protein folding. The unfolded protein response is activated in Pkd1 knockout kidneys and mutant cells, with GRP94 serving as a chaperone for PC2, and a novel HDAC6-GRP94-related axis likely regulating PC2 levels.
Article
Behavioral Sciences
Makoto Kimura, Yasunori Oda, Yuki Hirose, Hiroshi Kimura, Kouhei Yoshino, Tomihisa Niitsu, Nobuhisa Kanahara, Yukihiko Shirayama, Kenji Hashimoto, Masaomi Iyo
Summary: This study investigated the role of antipsychotic-induced neuronal damage in the development of dopamine supersensitivity state (DSS). The results showed that DSS rats experienced striatal neuronal damage, and an upregulation of HSP-70 and the GLT-1/GS system in the CA3 region induced by HAL may be involved in the development of DSS. Further research is needed to understand why non-DSS rats did not suffer neuronal damage and to explore potential therapeutic strategies for treatment-resistant schizophrenia, dopamine supersensitivity psychosis, and tardive dyskinesia.
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
(2021)
Article
Peripheral Vascular Disease
Takeshi Tokudome, Kentaro Otani, Yuanjie Mao, Lars Jorn Jensen, Yuji Arai, Takahiro Miyazaki, Takashi Sonobe, James T. Pearson, Tsukasa Osaki, Naoto Minamino, Junji Ishida, Akiyoshi Fukamizu, Hayato Kawakami, Daisuke Onozuka, Kunihiro Nishimura, Mikiya Miyazato, Hirohito Nishimura
Summary: This study revealed the crucial role of vascular endothelial NPR1 in ANP-mediated blood pressure regulation. ANP lowers blood pressure by activating potassium channels and modulating G-protein signaling pathways in endothelial cells. Furthermore, ANP's blood pressure regulation depends on RGS2 in the acute phase but is independent of RGS2 in the chronic phase.
Article
Plant Sciences
Murugan Naveenarani, Huskur Kumaraswamy Mahadeva Swamy, Sakthivel Surya Krishna, Channappa Mahadevaiah, Ramanathan Valarmathi, Markandan Manickavasagam, Muthukrishnan Arun, Govindakurup Hemaprabha, Chinnaswamy Appunu
Summary: Phosphorus deficiency has a significant impact on plant growth and development. The use of promoters from phosphate transporter genes can enhance phosphate uptake abilities in genetically modified plants, leading to improved crop yields in phosphate-deficient soils. In this study, the EaPHT1;2 gene from Erianthus arundinaceus was isolated and characterized. The promoter region of EaPHT1;2 showed higher expression than the commonly used CaMV35S promoter. The short promoter sequence of EaPHT1;2 showed significant expression under low-Pi-stress conditions and holds promise for developing stress-tolerant transgenic crops.
Article
Multidisciplinary Sciences
Rajivgandhi Pachaiyappan, Elankurisil Arasan, Kannan Chandrasekaran
Summary: An improved gorilla troops optimizer (iGTO)-based fuzzy PD-(1+PI) (FPD-(1+PI)) structure is proposed for frequency control in a smart grid system. The performance of this method is demonstrated through experiments and comparisons under various operating conditions and cyberattacks.
Review
Physiology
Sean R. Notley, Duncan Mitchell, Nigel A. S. Taylor
Summary: This article introduces the foundational principles, mechanistic hypotheses, and supported facts about human thermoregulation both in the past and present. It emphasizes the physical and physiological principles underlying thermoregulation and its association with exercise and physiological responses. The article also discusses the adaptations that increase our tolerance to thermal stresses. It highlights the hypothalamus as the neural seat of thermoregulation, which regulates body temperature through control of thermoreceptors throughout the body. Finally, the article emphasizes that exercise-induced thermoregulation does not require the invocation of work factors to explain the stabilization of body temperature.
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Rolien Bosch, Marcella Petrone, Rosalin Arends, Paolo Vicini, Eric J. G. Sijbrands, Sven Hoefman, Nelleke Snelder
Summary: GLP-1 receptor agonists and dual GLP-1/glucagon receptor agonists improve glycaemic control and cause weight loss in patients with type 2 diabetes by enhancing insulin release, reducing caloric intake, and delaying gastric emptying. The 4GI model integrates various drug effects on glucose regulatory pathways and provides quantitative insights to support the development of novel therapies and combination strategies.
CPT-PHARMACOMETRICS & SYSTEMS PHARMACOLOGY
(2022)
Proceedings Paper
Computer Science, Theory & Methods
Krishma Prashar, Sahaj Saxena
Summary: This paper proposes a new model-based feedback regulation strategy for the treatment of Type 1 diabetes using the artificial pancreas, which shows good regulatory performances and robustness against model uncertainties. A PI controller is designed using the internal model control (IMC) approach, and satisfactory simulation studies are conducted on a virtual patient.
2021 SEVENTH INDIAN CONTROL CONFERENCE (ICC)
(2021)
Article
Humanities, Multidisciplinary
Louis Autin
Summary: Popular discourses on the princeps' body in the 1st century AD, often denigrated by ancient authors, actually have a political value. The plebs were concerned about the emperor's health, and by praising aristocrats for their physique, they could indirectly delegitimize the ruling power.
CAHIERS MONDES ANCIENS
(2022)
Article
Energy & Fuels
Carolina I. D. G. Bonan, Robson Tramontina, Monick W. dos Santos, Luiz E. Biazi, Lauren B. Soares, Isabela O. Pereira, Zaira B. Hoffmam, Natalia Coutoune, Fabio M. Squina, Diogo Robl, Jaciane L. Ienczak
Summary: S. passalidarum is a yeast with potential for biotransformation of sugars from lignocellulosic biomasses, capable of simultaneous production of ethanol and xylitol. Low glucose concentrations are necessary to promote higher xylitol production without affecting ethanol yield, making it the most interesting condition for simultaneous formation of both products.
BIOENERGY RESEARCH
(2022)
Article
Microbiology
Tamires A. Bitencourt, Otavio Hatanaka, Andre M. Pessoni, Mateus S. Freitas, Gabriel Trentin, Patrick Santos, Antonio Rossi, Nilce M. Martinez-Rossi, Lysangela L. Alves, Arturo Casadevall, Marcio L. Rodrigues, Fausto Almeida
Summary: In this study, the researchers investigated the role of extracellular vesicles (EVs) in fungal communication. They found that EVs from Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis can regulate the expression of target genes and phenotypic features. The researchers propose that fungal EVs act as potent message effectors, and understanding their effects and mechanisms of action could be used in antifungal therapies.
Article
Microbiology
Leonardo Martins-Santana, Monise Fazolin Petrucelli, Pablo R. Sanches, Nilce M. Martinez-Rossi, Antonio Rossi
Summary: The study reveals the importance of alternative splicing in generating isoforms of virulence factors in Trichophyton rubrum, a common causative agent of dermatophytosis. The transcription factor StuA plays a crucial role in coordinating the expression of peptidase genes through alternative splicing in the presence of keratin. These findings shed light on the physiological adaptations of fungal cells and the regulation of virulence traits in dermatophyte infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Carlos H. Lopes Rocha, Flaviane M. Galvao Rocha, Tamires A. Bitencourt, Maira P. Martins, Pablo R. Sanches, Antonio Rossi, Nilce M. Martinez-Rossi
Summary: The antidepressant sertraline (SRT) alone or in combination with caspofungin (CASP) exhibits antifungal activity against Trichophyton rubrum, targeting genes involved in cell wall and ergosterol biosynthesis. This combination shows potential in minimizing resistance and reducing virulence of the pathogen.
Article
Biochemistry & Molecular Biology
Diogenes G. da S. Fernandes, Graziella S. Capo, Aryane Tofanello, Carlos E. Castro, Bruno B. Foltran, Fabio M. Squina, Maria H. C. Santos, Romerio R. S. Silva, Claudener S. Teixeira, Wanius Garcia
Summary: This study reports the synthesis of silver/silver chloride nanoparticles (NPs) assisted by ConA and DVL. The NPs showed interactions with membrane carbohydrates and potential applications in selective recognition and biotechnological applications.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Agnes C. Pimentel, Marcelo V. Liberato, Joao Paulo L. Franco Cairo, Geizecler Tomazetto, Cesar A. Gandin, Mario de Oliveira Neto, Thabata M. Alvarez, Fabio M. Squina
Summary: This study evaluated the structural and functional properties of the individualized GH5 and Calx-beta domains of CelE2 by subcloning and heterologously expressing them in E. coli. The results showed that the denaturation temperature of the catalytic domain (CelE21-381) decreased compared to the full-length CelE2, and the Calx-beta domain (CelE2382-477) was unfolded without attachment to the catalytic core. The deletion of the Calx-beta domain increased the activity of the catalytic domain towards the insoluble substrate Avicel, without altering other important functional properties.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Robson Tramontina, Iara Ciancaglini, Ellen K. B. Roman, Micaela G. Chacon, Thamy L. R. Correa, Neil Dixon, Timothy D. H. Bugg, Fabio Marcio Squina
Summary: The biorefinery concept offers an eco-friendly, cost-effective, and renewable alternative to petrochemical-based production by utilizing biomass for the production of fuels and chemicals. The hydroxycinnamic acid fraction of lignocellulosic biomass is an untapped source of aromatic molecules that can be converted into high-value products with industrial applications, such as flavor and fragrance sector and pharmaceuticals. This review discusses various biochemical pathways for the biocatalytic conversion of hydroxycinnamic acids ferulic, caffeic, and p-coumaric acid into high-value molecules in the development of a biorefinery concept.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Microbiology
Leonardo Martins-Santana, Caroline Patini Rezende, Antonio Rossi, Nilce Maria Martinez-Rossi, Fausto Almeida
Summary: Fungal infections, especially those caused by drug-resistant fungi, pose a serious global health concern. Candida, Aspergillus, and Cryptococcus are some of the fungi that cause severe and systemic infections, with high mortality rates. Understanding the immunopathogenesis of fungal diseases and finding ways to prevent dissemination and deaths caused by fungal infections are crucial. This review highlights the importance of drug resistance in clinically significant fungi and discusses strategies to combat these infections.
Article
Microbiology
Bruna Aline Cantelli, Gabriela Gonzalez Segura, Tamires Aparecida Bitencourt, Mariana Heinzen de Abreu, Monise Fazolin Petrucelli, Kamila Peronni, Pablo Rodrigo Sanches, Rene Oliveira Beleboni, Wilson Araujo da Silva Junior, Nilce Maria Martinez-Rossi, Mozart Marins, Ana Lucia Fachin
Summary: This study analyzed the transcriptome of THP-1 cells co-cultured with inactivated germinated T. rubrum conidia to characterize deep infection. Results showed increased release of IL-12 during co-culture, while the other cytokines remained unchanged. Gene expression analysis identified 83 modulated genes involved in signal transduction, cell communication, and immune response pathways. The study revealed the ability of macrophages to modulate the immune response and release proinflammatory cytokines, providing clues for exploring molecular targets in antifungal therapies involving immune system activation.
Article
Microbiology
Flaviane M. Galvao-Rocha, Carlos H. L. Rocha, Maira P. Martins, Pablo R. Sanches, Tamires A. Bitencourt, Matthew S. Sachs, Nilce M. Martinez-Rossi, Antonio Rossi
Summary: SRT alters gene expression related to fungal cell wall and plasma membrane stability, energy metabolism, detoxification, and defense against oxidative stress in T. rubrum, providing potential targets for its use in treating dermatophytosis.
Article
Biotechnology & Applied Microbiology
Nathalia Vilela, Geizecler Tomazetto, Thiago Augusto Goncalves, Victoria Sodre, Gabriela Felix Persinoti, Eduardo Cruz Moraes, Arthur Henrique Cavalcante de Oliveira, Stephanie Nemesio da Silva, Taicia Pacheco Fill, Andre Damasio, Fabio Marcio Squina
Summary: This study characterized the lignin-degrading ability of a novel Rhodosporidium fluviale strain LM-2 using multi-omic strategies. The strain exhibited a set of enzymes related to lignin depolymerization and aromatic compound catabolism, and two catabolic routes for producing 4-vinyl guaiacol and vanillin were identified. This research expands our understanding of ligninolytic metabolism in a non-conventional yeast and provides important clues for lignin valorization in other systems.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
F. Squina, J. P. Franco Cairo, R. Tramontina
Article
Microbiology
Tamires A. Bitencourt, Otavio Hatanaka, Andre M. Pessoni, Mateus S. Freitas, Gabriel Trentin, Patrick Santos, Antonio Rossi, Nilce M. Martinez-Rossi, Lysangela L. Alves, Arturo Casadevall, Marcio L. Rodrigues, Fausto Almeida
Summary: This study investigates the role of fungal extracellular vesicles (EVs) as potent message effectors in regulating gene expression and phenotypic features in pathogenic fungi.
