Review
Biochemistry & Molecular Biology
Morgana K. Kellogg, Sarah C. Miller, Elena B. Tikhonova, Andrey L. Karamyshev
Summary: Signal recognition particle (SRP) is an RNA and protein complex found across all domains of life, with varying compositions and functions in different organisms. Its main function involves targeting proteins to the endoplasmic reticulum and protecting the mRNA of secretory proteins from degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sayandeep Gupta, Mousam Roy, Dhritiman Dey, Koustav Bhakta, Arghya Bhowmick, Dhrubajyoti Chattopadhyay, Abhrajyoti Ghosh
Summary: The study demonstrates the important role of archaeal 7S RNA in stabilizing the SRP54$FtsY targeting complex, with further assistance from SRP19. These results suggest that archaeal 7S RNA and SRP19 reinforce the targeting complex to execute protein translocation through modulation of its conformation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Editorial Material
Genetics & Heredity
Morgana K. Kellogg, Elena B. Tikhonova, Andrey L. Karamyshev
Summary: The signal recognition particle (SRP) is an essential ribonucleoprotein complex involved in protein synthesis and its dysfunction can lead to various diseases.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Shuiling Ji, Bernhard Grimm, Peng Wang
Summary: This study reveals the distinct roles of cpSRP43 and cpSRP54 in optimizing the function of the major PORB isoform in Arabidopsis, with the former stabilizing the enzyme and providing appropriate amounts of PORB and the latter enhancing its binding to the thylakoid membrane to ensure adequate metabolic flux.
Editorial Material
Biochemistry & Molecular Biology
Oliver Weichenrieder
Summary: In this study, Juaire et al. investigated disease-associated variants of the SRP54 GTPase using X-ray crystallography, biophysical tools, and cell-based assays. They demonstrated that defects in SRP-mediated protein secretion can explain phenotypes of severe neutropenia with Shwachman-Diamond-syndrome-like symptoms.
Article
Biochemistry & Molecular Biology
Elena B. Tikhonova, Sneider Alexander Gutierrez Guarnizo, Morgana K. Kellogg, Alexander Karamyshev, Igor M. Dozmorov, Zemfira N. Karamysheva, Andrey L. Karamyshev
Summary: Regulation of Aberrant Protein Production (RAPP) is a protein quality control mechanism in mammalian cells. It degrades the mRNA of nascent proteins that cannot associate with their natural interacting partners. Depletion of human SRP54, an SRP subunit, triggers RAPP and leads to decreased expression of mRNAs encoding secretory and membrane proteins. The loss of SRP54 also results in the upregulation of a specific network of HSP70/40/90 chaperones, increased ribosome-associated ubiquitination, and changes in the expression of RPS27 and RPS27L, indicating ribosome rearrangement.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Sarah M. Hernandez, Elena B. Tikhonova, Kristen R. Baca, Fanpeng Zhao, Xiongwei Zhu, Andrey L. Karamyshev
Summary: The study found that SRP and AGO2 proteins are involved in the control of alpha Syn expression in PD, with reduced expression of AGO2 in PD. SRP was shown for the first time to be involved in mRNA protection of alpha Syn, revealing the potential roles of these factors in the pathogenesis of PD.
Article
Plant Sciences
Tingting Zhang, Xiangyu Dong, Xin Yuan, Yuanyuan Hong, Lingling Zhang, Xuan Zhang, Shuxia Chen
Summary: This study identified the candidate gene CsSRP43 in a spontaneous cucumber mutant, revealing its crucial role in leaf color and photosynthesis pathways. Through gene cloning and sequence analysis, the study provided insights into the molecular mechanisms underlying leaf color formation in cucumber.
HORTICULTURE RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Markus Hopfler, Ramanujan S. Hegde
Summary: Cells regulate mRNA processing, localization, and stability to ensure accurate gene expression. Recent discoveries have uncovered co-translational mechanisms that modulate mRNA translation, localization, processing, and stability. These mechanisms operate by recognizing the nascent protein and directing mRNA fate, allowing cells to dynamically regulate their transcriptomes in response to cellular changes.
Article
Plant Sciences
Yanwei Wang, Zhimin Yang, Meng Zhang, Pengfei Ai
Summary: This study identified the crucial role of the rice SSA1 gene in chloroplast biogenesis. SSA1 regulates early chloroplast development and is involved in RNA editing and intron splicing. These findings contribute to the understanding of molecular mechanisms underlying chloroplast generation.
Article
Biochemical Research Methods
A. J. Harris, Aaron David Goldman
Summary: The study investigates the early evolutionary history of cellularity, focusing on the roles of cell membranes, proteins, and the signal recognition particle (SRP) system in embedding and secreting proteins. The results suggest that the last universal common ancestor (LUCA) had an SRP system and SecY channel similar to present organisms, with a more rudimentary system in an earlier ancestor. The ancestral Ffh/FtsY protein likely emerged around the completion of the genetic code.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Julia Oswald, Robert Njenga, Ana Natriashvili, Pinku Sarmah, Hans-Georg Koch
Summary: The spatial and temporal coordination of protein transport is crucial for bacterial adaptation to different environmental conditions. The SecYEG translocon plays a key role in bacterial protein transport, with its ability to interact with multiple targeting factors, chaperones, and accessory proteins influencing its ability to transport a variety of substrates.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Joori Park, Jeeyoon Chang, Hyun Jung Hwang, Kwon Jeong, Hyuk-Joon Lee, Hongseok Ha, Yeonkyoung Park, Chunghun Lim, Jae-Sung Woo, Yoon Ki Kim
Summary: This study identified a surveillance pathway in which pioneer translation ensures proper targeting of endoplasmic reticulum and mitochondrial proteins, preventing abnormal protein folding and cytosolic stress response.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Hematology
Monika I. Linder, Yoko Mizoguchi, Sebastian Hesse, Gergely Csaba, Megumi Tatematsu, Marcin Lyszkiewicz, Natalia Zietara, Tim Jeske, Maximilian Hastreiter, Meino Rohlfs, Yanshan Liu, Piotr Grabowski, Kaarin Ahomaa, Daniela Maier-Begandt, Marko Schwestka, Vahid Pazhakh, Abdulsalam I. Isiaku, Brenda Briones Miranda, Piers Blombery, Megumu K. Saito, Ejona Rusha, Zahra Alizadeh, Zahra Pourpak, Masao Kobayashi, Nima Rezaei, Ekrem Unal, Fabian Hauck, Micha Drukker, Barbara Walzog, Juri Rappsilber, Ralf Zimmer, Graham J. Lieschke, Christoph Klein
Summary: This study discovered two novel genetic defects in SRPRA and SRP19 genes, which are involved in the differentiation of neutrophil granulocytes. Proteome analysis of neutrophil granulocytes from patients with variants in SRP genes revealed global and specific proteome aberrations. In vitro and in vivo experiments confirmed the crucial role of SRP-dependent protein processing, intracellular trafficking, and homeostasis in neutrophil granulocyte differentiation.
Article
Biochemistry & Molecular Biology
Xiuming Li, Wenzhen Luo, Wen Zhou, Xiaopeng Yin, Xuemei Wang, Xiujin Li, Chenchen Jiang, Qingqing Zhang, Xiaojing Kang, Aihong Zhang, Yi Zhang, Congming Lu
Summary: The study revealed the importance of PPR proteins in stabilizing RNA transcripts by binding to them and inhibiting RNA endonuclease activity, thereby modulating gene expression levels in chloroplasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Bjoern Walter, Athina Hristou, Marc M. Nowaczyk, Danja Schuenemann
BIOCHEMICAL JOURNAL
(2015)
Article
Biochemistry & Molecular Biology
Beatrix Duenschede, Chantal Traeger, Christine Vera Schoeder, Dominik Ziehe, Bjoern Walter, Silke Funke, Eckhard Hofmann, Danja Schuenemann
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Plant Sciences
Bjoern Walter, Thomas Pieta, Danja Schuenemann
Frontiers in Plant Science
(2015)
Review
Plant Sciences
Dominik Ziehe, Beatrix Duenschede, Danja Schuenemann
PHOTOSYNTHESIS RESEARCH
(2018)
Article
Plant Sciences
Anja Schneider, Iris Steinberger, Henning Strissel, Hans-Henning Kunz, Nikolay Manavski, Joerg Meurer, Gabi Burkhard, Sabine Jarzombski, Danja Schuenemann, Stefan Geimer, Ulf-Ingo Fluegge, Dario Leister
Article
Multidisciplinary Sciences
Anna Schoenberg, Elena Bergner, Stefan Helm, Birgit Agne, Beatrix Duenschede, Danja Schuenemann, Mike Schutkowski, Sacha Baginsky
Article
Multidisciplinary Sciences
Dominik Ziehe, Beatrix Diinschede, Mira Zenker, Silke Funke, Marc M. Nowaczyk, Danja Schunemann
Article
Multidisciplinary Sciences
Jose G. Garcia-Cerdan, Ariel L. Furst, Kent L. McDonald, Danja Schuenemann, Matthew B. Francis, Krishna K. Niyogi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Athina Hristou, Ines Gerlach, Dominique S. Stolle, Jennifer Neumann, Annika Bischoff, Beatrix Duenschede, Marc M. Nowaczyk, Reimo Zoschke, Danja Schuenemann
Article
Microbiology
Beate Sauerbrei, Jan Arends, Danja Schunemann, Franz Narberhaus
JOURNAL OF BACTERIOLOGY
(2020)
Article
Multidisciplinary Sciences
Thilo Ruehle, Marcel Dann, Bennet Reiter, Danja Schuenemann, Belen Naranjo, Jan-Ferdinand Penzler, Tatjana Kleine, Dario Leister
Summary: The study reveals that PGRL1 and PGRL2 modulate PGR5 activity and stability, affecting the mediation process of cyclic electron flow (CEF).
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Bernd Ackermann, Beatrix Duenschede, Bjoern Pietzenuk, Bo Hojen Justesen, Ute Kraemer, Eckhard Hofmann, Thomas Guenther Pomorski, Danja Schuenemann
Summary: Members of the Oxa1/YidC/Alb3 protein family are involved in the insertion, folding, and assembly of membrane proteins in mitochondria, bacteria, and chloroplasts. The thylakoid membrane protein Alb3 interacts with ribosomes during posttranslational insertion of specific proteins, while Alb4, a homolog located in the same membrane, does not show ribosome binding. Alb3 contacts ribosomes via its C-terminal region and at least one additional binding site within its hydrophobic core.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jakob-Maximilian Keller, Maureen Julia Frieboes, Ludwig Joedecke, Sandrine Kappel, Natalia Wulff, Tobias Rindfleisch, Omar Sandoval-Ibanez, Ines Gerlach, Wolfram Thiele, Ralph Bock, Juergen Eirich, Iris Finkemeier, Danja Schuenemann, Reimo Zoschke, Mark Aurel Schoettler, Ute Armbruster
Summary: This study demonstrates the role of the DECREASED ELECTRON TRANSPORT AT PSII (DEAP2) assembly factor in the early assembly of photosystem II (PSII) in plants. It shows that DEAP2 works together with the PHOTOSYNTHESIS AFFECTED MUTANT 68 (PAM68) factor to facilitate the formation of the RC47 intermediate. The loss of both DEAP2 and PAM68 leads to a loss of functional PSII and the inability of plants to grow photoautotrophically.
Review
Biochemistry & Molecular Biology
Dominik Ziehe, Beatrix Duenschede, Danja Schuenemann
BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Magnus Alm Rosenblad, Chantal Traeger, Danja Schuenemann
PLANT SIGNALING & BEHAVIOR
(2013)