期刊
FEBS LETTERS
卷 582, 期 15, 页码 2155-2160出版社
WILEY
DOI: 10.1016/j.febslet.2008.05.033
关键词
hepatitis C virus; NS5B; L type pyruvate kinase; M2 type pyruvate kinase
Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.
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