Article
Chemistry, Multidisciplinary
Rajan Shrivastava, Subhendu Ghosh
Summary: Ion channels self-organize into clusters on cellular and organelle membranes, mutually modulating their gating behavior. A statistical mechanical model has been proposed to study the collective dynamics in ion channel clusters, successfully describing the multichannel gating behavior and self-organization.
Article
Chemistry, Multidisciplinary
Daniel A. Wirtz, Kevin C. Ludwig, Melina Arts, Carina E. Marx, Sebastian Krannich, Paul Barac, Stefan Kehraus, Michaele Josten, Beate Henrichfreise, Anna Mueller, Gabriele M. Koenig, Aaron J. Peoples, Anthony Nitti, Amy L. Spoering, Losee L. Ling, Kim Lewis, Max Cruesemann, Tanja Schneider
Summary: Hypeptin is a cyclodepsipeptide antibiotic that blocks bacterial cell wall biosynthesis by binding to multiple biosynthesis intermediates, forming a 2:1 complex. It exhibits potent activity against gram-positive pathogens and resistance to it does not readily develop. Detailed analysis of the hypeptin biosynthetic gene cluster supports a model for the synthesis of the octapeptide.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Materials Science, Multidisciplinary
Tun Naw Sut, Bo Kyeong Yoon, Won-Yong Jeon, Joshua A. Jackman, Nam-Joon Cho
Summary: Supported lipid bilayer (SLB) coatings, versatile cell-membrane-mimicking biointerfaces, have been expanded for medical and biotechnology applications, facilitating various studies and applications like biosensors, diagnostics, and antifouling surfaces.
APPLIED MATERIALS TODAY
(2021)
Article
Engineering, Biomedical
Yasin Ozturk, Aliakbar Ebrahimi, Araz Norouz Dizaji, Ozge Kaygusuz, Jayesh Arun Bafna, Mathias Winterhalter, Guven Cankaya, Cihan Darcan, Fatma Dogan Guzel
Summary: The study investigates the reproducible generation of micropores in the range of 50-100 microns in diameter in a Teflon film. The multi-pulse method is more convenient for lipid bilayer formation and has a higher success rate. Experimental results show that the lipid bilayer on Teflon pores can last for more than 33 hours.
BIOMEDICAL MICRODEVICES
(2021)
Article
Biochemistry & Molecular Biology
Diana Priyadarshini, Josip Ivica, Frances Separovic, Maurits R. R. de Planque
Summary: Many antimicrobial peptides (AMPs) are cationic host defence peptides (HDPs) that interact with microbial membranes, potentially serving as therapeutics to combat antibiotic resistance. Studying the effects of four AMPs and a bee toxin on different phospholipid membranes highlights the importance of using appropriate bilayer compositions and peptide concentrations for AMP research. The research confirms existing peptide-bilayer interaction models and emphasizes the significance of understanding AMP behavior in relation to lipid bilayers mimicking bacterial and human cell membranes.
BIOPHYSICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Joyce J. Wong, Zhongwen Chen, Jean K. Chung, Jay T. Groves, Theodore S. Jardetzky
Summary: Paramyxovirus membrane fusion requires an attachment protein binding to a host cell receptor and a fusion protein merging viral and host membranes. The dynamic events of NiV fusion activation involve G and ephrinB2 forming clusters, with ephrinB2 oligomerization being essential for F activation. Single-molecule tracking of F particles revealed an immobilized intermediate upon activation, and no stable F-G protein complexes were found.
Article
Biochemical Research Methods
Simona Notova, Nathan Cannac, Luca Rabagliati, Maeva Touzard, Josselin Mante, Yotam Navon, Liliane Coche-Guerente, Olivier Lerouxel, Laurent Heux, Anne Imberty
Summary: The cell wall is a crucial component of plant cells, providing mechanical resistance and flexibility. In this study, the researchers successfully assembled complete plant cell wall mimics by bridging pectin to the cellulose/hemicellulose network using an engineered chimeric protein. This artificial cell wall serves as a basis for the development of plant cell mechanical models and enhances understanding of the principles underlying various aspects of plant cells and tissues.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tsuyoshi Yoda, Yudai Yamada, Yukiyasu Chounan
Summary: The study demonstrates that thermal stress can induce dynamic shape changes in IVA-containing lipid vesicles, with larger vesicles and increasing IVA concentration resulting in less thermo-responsiveness. These findings suggest that the characteristics of IVA-containing membranes can be utilized for evaluating drink quality and may contribute to advancements in membrane technology and understanding physiological processes such as flavor sensation.
BIOPHYSICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Yiping Feng, Jiayong Lao, Jiale Zou, Zhiyu Zhu, Daguang Li, Guoguang Liu, Liang Mao
Summary: Understanding the interactions between nanomaterials and cell membranes is important for ecotoxicology and human health. In this study, the interaction between graphitic carbon nitride (CN) and red blood cells was investigated. It was found that CN causes rapid cell membrane rupture through a physical interaction mechanism rather than chemical oxidation. Additionally, CN was found to extract phospholipids, particularly phosphatidylcholine, from cell membranes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Mira Holzheimer, Jeffrey Buter, Adriaan J. Minnaard
Summary: The pathogen Mycobacterium tuberculosis has a thick cell envelope rich in Mtb-specific lipids, glycolipids, and glycans, which are directly involved in host-pathogen interactions, intracellular survival, and virulence. Synthesizing these components aids in studying Mtb immunology and has driven advancements in the field of organic synthesis.
Article
Engineering, Mechanical
Choon-Peng Chng, Yoel Sadovsky, K. Jimmy Hsia, Changjin Huang
Summary: The peroxidation of lipids with polyunsaturated fatty acid tails modulates the biophysical properties of bilayer membrane in a site-specific manner, influencing membrane structure and properties. Specifically, peroxidation at different sites leads to distinct changes in the membrane, with peroxidation in the bilayer interior causing membrane disruption and softening, while peroxidation near the membrane-water interface results in a more ordered and stiffer membrane.
EXTREME MECHANICS LETTERS
(2021)
Article
Chemistry, Physical
Chad I. Drexler, Jenee D. Cyran, Lauren J. Webb
Summary: The cell-penetrating peptide NAF-1 shows promise in selectively penetrating and destroying cancer cells. Its membrane penetration and translocation are influenced by the lipid composition of the model lipid bilayer vesicles, particularly the presence of phosphatidic acid.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Lukas Martin Wingen, Christina Braun, Marvin Rausch, Harald Gross, Tanja Schneider, Dirk Menche
Summary: This article reports detailed information on the design, strategies, and tactics for developing a novel synthetic sequence for farnesyl lipid I and II analogs. The modular route involves a three coupling strategy, including an efficient solid phase synthesis of the elaborate peptide fragment, and is efficiently applied for the convergent synthesis of 3-lipid I and II. Furthermore, the generality of this route is demonstrated by synthesizing 3-lipid I congeners specific to S. aureus and E. faecalis. All 3-lipid I and II building blocks are obtained in high purity with high spectroscopic resolution.
Article
Biochemistry & Molecular Biology
James C. S. Ho, Sartaj Ahmad Mir, Michele Cavalera, Parisa Esmaeili, Tuan Hiep Tran, Zandra Chew Yann, Thi Hien Tran, Arunima Chaudhuri, Anne K. Bendt, Markus R. Wenk, Catharina Svanborg
Summary: This study found that bladder cancer tissue has a higher abundance of unsaturated lipids, which can enhance the tumor cell response to alpha1-oleate, compared to healthy tissue. This highlights the functional importance of membrane lipid composition in tumor cell reactivity.
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Microbiology
Dominic Stephenson, Audrey Perry, Andrew Nelson, Ali E. Robb, Matthew F. Thomas, Stephen J. Bourke, John D. Perry, Amanda L. Jones
Summary: Among six different decontamination strategies, treatment with NALC-NaOH resulted in an average reduction in colony count of 87% for MABSC, while 4% NaOH caused a 98.3% average reduction. All treatments including NaOH showed significantly lower colony counts compared to direct culture. The viability of MAC was less affected by most decontamination treatments.
Article
Biophysics
Roland Benz, Claudio Piselli, Cezarela Hoxha, Cornelia Koy, Michael O. Glocker, Michel R. Popoff
Summary: Clostridium perfringens produces a variety of toxins, with one virulent toxin cCPB2 forming highly active cation-selective channels, leading to necrotizing enterocolitis. Further research is exploring the biological activity and phylogenetic relationship of cCPB2 and other toxins.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Article
Chemistry, Physical
Vinaya Kumar Golla, Claudio Piselli, Ulrich Kleinekathoefer, Roland Benz
Summary: This study investigates the permeation of fosfomycin through OprP and OprO channels in Pseudomonas aeruginosa bacteria using electrophysiology and molecular dynamics simulations. The results show that fosfomycin has higher binding affinity to OprP and OprO compared to fosmidomycin, and that arginine residues and lysine residue clusters play an important role in fosfomycin permeation. This study provides molecular insights on improving antibiotic permeation into Gram-negative bacteria, particularly resistant P. aeruginosa strains.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Eszter E. Najbauer, Kumar Tekwani Movellan, Karin Giller, Roland Benz, Stefan Becker, Christian Griesinger, Loren B. Andreas
Summary: In this study, the structure of VDAC1 was successfully determined in a near-native lipid bilayer environment using proton-detected fast magic-angle spinning NMR spectroscopy. Dynamics were found to play a crucial role in the gating behavior of the protein, and cholesterol was shown to stabilize the channel structure.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Letter
Microbiology
Iain C. Sutcliffe, David R. Arahal, Markus Goeker, Aharon Oren
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Biophysics
Claudio Piselli, Lorraine Benier, Cornelia Koy, Michael O. Glocker, Roland Benz
Summary: The cell wall of Rhodococcus corynebacteroides and Rhodococcus ruber contains cell wall channels formed by polypeptide subunits, which may belong to a large protein family (PF09203) that forms MspA-like cell wall channels. These proteins are able to form channels in lipid bilayers.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Letter
Microbiology
Aharon Oren, Markus Goeker, Richard L. Hahnke, Edward R. B. Moore, Iain C. Sutcliffe
NATURE MICROBIOLOGY
(2022)
Article
Microbiology
Vartul Sangal, Michael Goodfellow, Amanda L. Jones, Iain C. Sutcliffe
Summary: Opinion 106 of the Judicial Commission clarifies the nomenclature of certain bacterial taxa and proposes the reclassification of four rhodococcal species into a new genus called Prescottella.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Claudio Piselli, Vinaya Kumar Golla, Roland Benz, Ulrich Kleinekathoefer
Summary: This study focuses on the characterization of the lysine cluster in OprO, a porin protein in the outer membrane of Pseudomonas aeruginosa. The importance of lysine residues in ion conductance and phosphate ion flow was demonstrated through experiments and simulations. The results suggest that an improved understanding of lysine residues and ion mobility could lead to the development of more effective antibiotics for P. aeruginosa.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Chemistry, Medicinal
Abhishek Acharya, Ishan Ghai, Claudio Piselli, Jigneshkumar Dahyabhai Prajapati, Roland Benz, Mathias Winterhalter, Ulrich Kleinekathoefer
Summary: In this study, the molecular mechanism of an antibiotic permeating through a bacterial channel is revealed, with a focus on the role of conformational dynamics of the constriction loop. The dynamics of the L3 loop, specifically the F118 to S125 segment, at the constriction regions of the OmpF porin is investigated using temperature accelerated sliced sampling approach. The importance of hydrogen-bond stabilization in the conformational dynamics of the L3 loop is demonstrated through single channel electrophysiology experiments and applied-field simulations. The findings suggest that conformational dynamics of the constriction loop may play a general role in antibiotic permeation through porins.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Review
Oncology
Samaneh Eickelschulte, Anja Lisa Riediger, Arlou Kristina Angeles, Florian Janke, Stefan Duensing, Holger Sueltmann, Magdalena Goertz
Summary: Prostate cancer, a heterogeneous disease, lacks accurate diagnostic biomarkers such as prostate-specific antigen. Novel tissue and liquid biopsy biomarkers have emerged, providing potential complementary options for the diagnosis of heterogeneous tumors. Current liquid biopsy approaches offer characteristics of tumors, but the spatial heterogeneity of tumors highlights the need for additional diagnostic and prognostic options.
Article
Biochemistry & Molecular Biology
Delsi Altenhofen, Jenny Minh-An Khuong, Tanja Kuhn, Sandra Lebek, Sarah Goerigk, Katharina Kaiser, Christian Binsch, Kerstin Griess, Birgit Knebel, Bengt-Frederik Belgardt, Sandra Cames, Samaneh Eickelschulte, Torben Stermann, Axel Rasche, Ralf Herwig, Juergen Weiss, Heike Vogel, Annette Schuermann, Alexandra Chadt, Hadi Al-Hasani
Summary: By using a combined approach, including positional cloning, gene expression analyses, and in silico predictions, we identified the novel T2D-associated gene Nbg15 and its potential causative genes Cbx6, Fam135b, and Kdelr3, which are involved in the dysfunction of pancreatic beta cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Endocrinology & Metabolism
A. D. Bedou, T. Stermann, S. Eickelschulte, A. Chadt, H. Al-Hasani
Article
Infectious Diseases
M. Palmer, I. Sutcliffe, S. N. Venter, B. P. Hedlund
Summary: The International Code of Nomenclature of Prokaryotes (ICNP) currently requires pure cultures as the only acceptable nomenclatural type for species. However, this requirement is rigid and limits the ability to name a large proportion of microbial biodiversity. The availability of genome sequence data allows for a new category of nomenclatural type that includes both cultivated and uncultivated microorganisms, providing a unified nomenclature for archaea and bacteria.
NEW MICROBES AND NEW INFECTIONS
(2022)