期刊
FEBS JOURNAL
卷 279, 期 4, 页码 563-571出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1742-4658.2011.08445.x
关键词
drug discovery; fragment-based ligand discovery; leukaemia; PPI stabilization; protein-protein interactions; X-ray crystallography
资金
- IMPRS
- BMBF [GO-Bio 0313873]
- BayerCrop Science
- Bayer Healthcare
- MerckSerono
- MSD
Myeloid leukaemia factor 1 (MLF1) binds to 14-3-3 adapter proteins by a sequence surrounding Ser34 with the functional consequences of this interaction largely unknown. We present here the high-resolution crystal structure of this binding motif [MLF1(2942)pSer34] in complex with 14-3-3e and analyse the interaction with isothermal titration calorimetry. Fragment-based ligand discovery employing crystals of the binary 14-3-3e/MLF1(2942)pSer34 complex was used to identify a molecule that binds to the interface rim of the two proteins, potentially representing the starting point for the development of a small molecule that stabilizes the MLF1/14-3-3 proteinprotein interaction. Such a compound might be used as a chemical biology tool to further analyse the 14-3-3/MLF1 interaction without the use of genetic methods.
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