Article
Biochemistry & Molecular Biology
Anish Kumar Mondal, Kusum Lata, Mahendra Singh, Shamaita Chatterjee, Aakanksha Chauhan, Sindhoora Puravankara, Kausik Chattopadhyay
Summary: This article discusses the mechanism of pore formation by pore-forming toxins (PFTs), with a particular focus on the mechanistic details contributed by cryo-EM-based structural studies.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Food Science & Technology
Vincent Deruelle, Alice Berry, Stephanie Bouillot, Viviana Job, Antoine P. Maillard, Sylvie Elsen, Philippe Huber
Summary: ExlA is a highly virulent pore-forming toxin secreted by outlier strains of Pseudomonas aeruginosa, accumulating at specific points of the outer membrane when intracellular levels of c-di-GMP are high. Research shows that ExlA is poorly secreted in liquid culture, but highly detectable in mice infected with an exlA+ strain.
Review
Food Science & Technology
Kazunori Murase
Summary: This review provides an overview of the current knowledge about ClyA, including its encoding gene, transcriptional regulation, protein secretion pathway, and assembly mechanism. It highlights the potential applications of ClyA in biotechnology, such as nanopore-based technologies, vaccine development, and anti-tumor agents.
Review
Chemistry, Multidisciplinary
Dehua Pei
Summary: This paper introduces a previously unrecognized membrane translocation mechanism called the vesicle budding-and-collapse (VBC) mechanism. Through VBC, biomolecules can autonomously translocate across cell membranes. The study found that CPPs and bacterial protein toxins can escape endosomes through the VBC mechanism, and proposed that VBC may be the mechanism that drives the bacterial TAT and eukaryotic UPS systems.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Cristian Rocha-Roa, Juan David Orjuela, Chad Leidy, Pilar Cossio, Camilo Aponte-Santamaria
Summary: The presence of cardiolipin can alter the structure and order of bacterial-like membranes, making them less susceptible to mechanical changes and preventing the formation of membrane pores.
Review
Food Science & Technology
Sylvie Nonin-Lecomte, Laurence Fermon, Brice Felden, Marie-Laure Pinel-Marie
Summary: Bacterial type I toxin-antitoxin systems consist of stable toxic proteins and unstable RNA antitoxins, regulating growth arrest or cell death. These systems, with small hydrophobic peptides, impact intracellular ATP levels and play roles in plasmid maintenance, environmental stress adaptation, or persister cell formation.
Review
Food Science & Technology
Yajuan Li, Yuelong Li, Hylemariam Mihiretie Mengist, Cuixiao Shi, Caiying Zhang, Bo Wang, Tingting Li, Ying Huang, Yuanhong Xu, Tengchuan Jin
Summary: Pore-forming toxins (PFTs) are a major virulence factor of many pathogenic bacteria, with well-characterized structural basis and interactions with host cell membranes. Understanding the mechanisms of PFTs can contribute to the development of novel therapeutic strategies and combat bacterial antibiotic resistance.
Article
Biochemistry & Molecular Biology
Huijie Zhang, Weirong Zeng, Ming-Ming Zhao, Jiali Wang, Qiquan Wang, Ting Chen, Yuyan Zhang, Wenhui Lee, Shenghan Chen, Yun Zhang, Xinqiang Lan, Yang Xiang
Summary: This study reveals the potential anti-bacterial function of the af-PFP protein family. LIN-24, a member of this family, plays an important role in resisting bacterial infection by activating specific immune responses. These findings suggest that the af-PFP family may have an important protective role in combating microbial infections.
Article
Microbiology
Kathleen R. Nicholson, Patricia A. Champion
Summary: This article reviews the mechanisms of protein localization and transport in bacteria, highlighting the conserved themes of regulation and environmental sensing in bacterial secretion systems.
Article
Biotechnology & Applied Microbiology
Tingting Guo, Yue Cui, Lingwen Zhang, Xiaoning Xu, Zhenxiang Xu, Jian Kong
Summary: This study demonstrates that phage-encoded holin can be used to enhance extracellular protein yields in both Gram-negative and -positive bacteria. Overexpression of holin increases the secretion of target proteins and suggests an alternative pathway for protein secretion.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Food Science & Technology
Yu Larpin, Herve Besancon, Victoriia S. Babiychuk, Eduard B. Babiychuk, Rene Koffel
Summary: The research findings demonstrate that small pore-forming toxins, such as lysenin and aerolysin, have different effects on cells, with lysenin causing calcium influx and activating membrane repair mechanisms, while aerolysin does not. Cell resistance is influenced by factors such as binding efficiency, pore localization, and the activation of membrane repair mechanisms.
Review
Food Science & Technology
Drusilla L. Burns
Summary: Bordetella pertussis relies on a two-step secretion process to produce and secrete pertussis toxin, involving transport across both the inner and outer membranes of the bacterial cell. The specialized Ptl transporter is essential for the outer membrane transport of PT, distinguishing it from canonical type IV substrate transport.
Article
Multidisciplinary Sciences
Israel Castillo-Juarez, Blanca Esther Blancas-Luciano, Rodolfo Garcia-Contreras, Ana Maria Fernandez-Presas
Summary: Antimicrobial peptides (AMPs) have broad antimicrobial activity and can inhibit microbial growth. In addition to their bactericidal effects, AMPs also have anti-virulence activity against pathogenic bacteria, including those in biofilms. Understanding the mechanisms behind AMPs' anti-virulence properties is important for developing alternative therapeutic strategies to combat antibiotic resistance.
Article
Biochemistry & Molecular Biology
Anish Kumar Mondal, Paras Verma, Nayanika Sengupta, Somnath Dutta, Shashi Bhushan Pandit, Kausik Chattopadhyay
Summary: This study demonstrates the crucial role of a key tyrosine residue (Y321) in the hinge region of the pore-forming motif of Vibrio cholerae cytolysin in regulating membrane binding, structural rearrangements, oligomerization, and pore-formation, providing insights into the molecular mechanism of beta-barrel pore-forming toxins.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Magdalena Kulma, Gregor Anderluh
Summary: Pore-forming proteins (PFPs) alter membrane permeability by forming transmembrane pores, destroying target cells and potentially manipulating cellular functions. Recent evidence suggests that PFPs also induce extensive membrane reorganization, including lateral rearrangement and deformation, which can disrupt cell function and lead to death. These modifications are also part of a membrane repair system that protects cells from the lethal effects of pore formation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Microbiology
Julian Trouillon, Michel Ragno, Victor Simon, Ina Attree, Sylvie Elsen
Summary: Transcription factors (TFs) play a crucial role in bacterial response to new environmental conditions. A study on a family of eight TFs in Pseudomonas aeruginosa revealed XRE-cupin TFs act as inhibitors of neighboring genes, forming functional units for condition-specific metabolic pathways. Growth phenotypes of isogenic mutants highlighted new roles for PauR and PA0535 in polyamines and arginine metabolism. Phylogenetic analysis across bacterial species showed wide diversity in metabolic regulatory modules.
Article
Biochemistry & Molecular Biology
Oriane Moyne, Florence Castelli, Dominique J. Bicout, Julien Boccard, Boubou Camara, Benoit Cournoyer, Eric Faudry, Samuel Terrier, Dalil Hannani, Sarah Huot-Marchand, Claire Leger, Max Maurin, Tuan-Dung Ngo, Caroline Plazy, Robert A. Quinn, Ina Attree, Francois Fenaille, Bertrand Toussaint, Audrey Le Gouellec
Summary: The study used metabolomics analysis to explore the adaptive evolution of Pseudomonas aeruginosa in chronically infected cystic fibrosis patients, revealing significant associations between metabotypes, antibiotic resistance, virulence phenotypes, and clinical exacerbations. This identified promising biomarkers and therapeutic targets for difficult-to-treat P.a infections.
Article
Biochemistry & Molecular Biology
Samira Zouhir, Carlos Contreras-Martel, Daniel Maragno Trindade, Ina Attree, Andrea Dessen, Pauline Macheboeuf
Summary: The study found that in Pseudomonas aeruginosa, MagC may act as a link between MagD macroglobulin and peptidoglycan, providing stability and regulation for the entire complex.
Article
Multidisciplinary Sciences
Vincent Deruelle, Stephanie Bouillot, Viviana Job, Emmanuel Taillebourg, Marie-Odile Fauvarque, Ina Attree, Philippe Huber
Summary: The phospholipase ExoU from Pseudomonas aeruginosa acts on plasma membrane lipids in infected cells, causing membrane rupture and host cell necrosis. Once injected into the host cytoplasm, ExoU requires a host chaperone found on secretory vesicles to reach the plasma membrane and exert its phospholipase activity.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Alexandre Martins, Carlos Contreras-Martel, Manon Janet-Maitre, Mayara M. Miyachiro, Leandro F. Estrozi, Daniel Maragno Trindade, Caique C. Malospirito, Fernanda Rodrigues-Costa, Lionel Imbert, Viviana Job, Guy Schoehn, Ina Attree, Andrea Dessen
Summary: The elongasome, or Rod system, is a protein complex that controls cell wall formation in rod-shaped bacteria. MreC, a membrane-associated component of the elongasome, interacts with the cytoskeletal element MreB and regulates cell wall biosynthesis enzymes through self-association, contributing to the regulation of elongasome activity.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Julian Trouillon, Kook Han, Ina Attree, Stephen Lory
Summary: This study identified Hfq-interacting RNAs in different strains of Pseudomonas aeruginosa using RIP-seq technology. The results showed that most interactions are not conserved among different strains. Strain-specific Hfq targets were identified, including previously undescribed sRNAs.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Viviana Job, Laura Gomez-Valero, Adele Renier, Christophe Rusniok, Stephanie Bouillot, Viviane Chenal-Francisque, Erwan Gueguen, Annie Adrait, Mylene Robert-Genthon, Katy Jeannot, Peter Panchev, Sylvie Elsen, Marie-Odile Fauvarque, Yohann Coute, Carmen Buchrieser, Ina Attree
Summary: This study demonstrates the role of TPS toxins in bacteria-insect interactions in environmental Pseudomonas, and suggests that differences in CTDs may determine their specificity and mode of action towards eukaryotic cells.
Article
Microbiology
Manon Janet-Maitre, Stephane Pont, Frerich M. Masson, Serena Sleiman, Julian W. Trouillon, Mylene Robert-Genthon, Benoit Gallet, Chantal Dumestre-Perard, Sylvie Elsen, Christine Moriscot, Bart W. Bardoel, Suzan H. M. Rooijakkers, Francois Cretin, Ina Attree
Summary: This study found that multiple factors, including bacterial surface-attached molecules and adaptation to stress, as well as intracellular polyphosphates and biotin, significantly affect Pseudomonas aeruginosa's ability to withstand membrane attack complex (MAC)-dependent killing in human plasma. These findings highlight the importance of understanding the complex interaction between bacterial pathogens and the human immune system for developing effective antibacterial strategies.
Article
Multidisciplinary Sciences
Karina T. Shirakawaa, Fernanda Angelica Salaa, Mayara M. Miyachiroc, Viviana Jobc, Daniel Maragno Trindadea, Andrea Dessena, Christoph Mayer, Eberhard Karls
Summary: The biosynthesis of peptidoglycan (PG) in bacteria involves a multimembered complex of Mur enzymes. Genomic analysis of over 140 bacterial genomes revealed various forms of Mur enzyme fusions among different species, with Proteobacteria carrying the highest number. The crystal structure of the MurE-MurF chimera from Bordetella pertussis showed a head-to-tail elongated architecture, supported by an interconnecting hydrophobic patch. Fluorescence polarization assays demonstrated the interaction of MurE-MurF with other Mur ligases, supporting the existence of a Mur complex in the cytoplasm.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Y. T. Candace Ho, Joe A. Kaczmarski, Julien Tailhades, Thierry Izore, David L. Steer, Ralf B. Schittenhelm, Manuela Tosin, Colin J. Jackson, Max J. Cryle
Summary: Nonribosomal peptide synthetases play a significant role in producing essential peptide natural products, with carrier proteins as their core component. By replacing the CP substrate thioesters with stabilized ester analogues, active condensation domain complexes are formed, while amide stabilization leads to non-functional complexes.
CHEMICAL COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Sarah Bonhomme, Andrea Dessen, Pauline Macheboeuf
Summary: NRPSs are multienzymes that produce complex natural metabolites with applications in medicine and agriculture. They consist of multiple catalytic domains and non-catalytic carrier protein domains, allowing flexibility in catalyzing and transporting products. Recent studies providing structural views of multi-domain NRPSs have enhanced understanding of their catalytic cycle.
Article
Chemistry, Multidisciplinary
Jennifer A. E. Payne, Ketav Kulkarni, Thierry Izore, Alex J. Fulcher, Anton Y. Peleg, Marie-Isabel Aguilar, Max J. Cryle, Mark P. Del Borgo
Summary: A new antimicrobial material was designed, synthesised and characterised by the self-assembly of two distinct beta-peptide monomers, which showed inhibitory effects on drug-resistant bacteria like MRSA. This study provides insights into the design of peptide-based supramolecular assemblies with antibacterial activity.
NANOSCALE ADVANCES
(2021)