Article
Chemistry, Multidisciplinary
Sara Bertuzzi, Francesca Peccati, Sonia Serna, Raik Artschwager, Simona Notova, Michel Thepaut, Gonzalo Jimenez-Oses, Franck Fieschi, Niels C. Reichardt, Jesus Jimenez-Barbero, Ana Arda
Summary: The molecular recognition features of LSECtin towards asymmetric N-glycans were scrutinized by NMR and compared to glycan microarrays. It was found that both asymmetric LDN3 and LDN6 N-glycans are recognized by LSECtin with similar affinities in solution, contrary to the results obtained on microarrays, where only LDN6 was efficiently recognized by the lectin.
ACS CENTRAL SCIENCE
(2022)
Article
Microbiology
Lillian F. Zhang, Bernd Lepenies, Sayuri Nakamae, Briana M. Young, Renato L. Santos, Manuela Raffatellu, Brian A. Cobb, Hirotaka Hiyoshi, Andreas J. Baeumler
Summary: Salmonella enterica subspecies enterica serovar Typhi is the causative agent of typhoid fever, and the recent emergence of antibiotic-resistant S. Typhi strains highlights the importance of vaccination. The study identified the human C-type lectin receptor DC-SIGN as the receptor for the Vi capsular polysaccharide, which contributes to the pathogenesis of typhoid fever.
Review
Biology
Nader Rahimi
Summary: The interaction between the S protein of SARS-CoV-2 and CD209 family proteins plays a crucial role in the virus's transmission and infection process, potentially leading to dysregulation of the host immune response and affecting various organs, including the cardiovascular system. Understanding the mechanisms behind this interaction could provide insights for therapies and vaccine development.
Article
Chemistry, Multidisciplinary
Robert Wawrzinek, Eike-Christian Wamhoff, Jonathan Lefebre, Mareike Rentzsch, Gunnar Bachem, Gary Domeniconi, Jessica Schulze, Felix F. Fuchsberger, Hengxi Zhang, Carlos Modenutti, Lennart Schnirch, Marcelo A. Marti, Oliver Schwardt, Maria Braeutigam, Monica Guberman, Dirk Hauck, Peter H. Seeberger, Oliver Seitz, Alexander Titz, Beat Ernst, Christoph Rademacher
Summary: Dendritic cells play a crucial role in coordinating immune responses, with new research showing potential for improved targeting of specific dendritic cell subtypes. This discovery has important implications for immunotherapy and anti-infectives.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Medicinal
Jonathan Cramer, Butrint Aliu, Xiaohua Jiang, Timothy Sharpe, Lijuan Pang, Adrian Hadorn, Said Rabbani, Beat Ernst
Summary: Research has shown that mannose-functionalized poly-L-lysine glycoconjugates efficiently inhibit viral attachment and prevent virus binding to DC-SIGN receptors. After treatment, these polymers can lead to prolonged receptor internalization and inhibition of virus binding for up to 6 hours.
Article
Immunology
Camille Niveau, Eleonora Sosa Cuevas, Benoit Roubinet, Mylene Pezet, Michel Thepaut, Stephane Mouret, Julie Charles, Franck Fieschi, Ludovic Landemarre, Laurence Chaperot, Philippe Saas, Caroline Aspord
Summary: Dendritic cell subsets play a crucial role in shaping anti-tumour immunity. The study found that melanoma tumour cells display aberrant glycan motifs which can be sensed by dendritic cells through specific receptors. This interaction may lead to abnormal immune responses. The study suggests that targeting the glycan/receptor axis could be a promising strategy for improving cancer immunotherapy.
Article
Biochemistry & Molecular Biology
Marije E. E. Kuipers, D. Linh Nguyen, Angela van Diepen, Lynn Mes, Erik Bos, Roman I. I. Koning, Esther N. M. Nolte-'t Hoen, Hermelijn H. H. Smits, Cornelis H. H. Hokke
Summary: Adult schistosome worms release extracellular vesicles (EVs) with a specific glycosylation profile that interacts with the host through different receptors compared to the EVs released by the schistosomula stage, indicating their distinct roles in the parasite's life-stage specific interactions with the host.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Yujun Kim, Ji Young Hyun, Injae Shin
Summary: This review discusses the applications of glycan microarrays in studying glycan-protein interactions. It describes the methods for preparing glycan probes and constructing glycan microarrays, and highlights their use in profiling glycan-binding patterns and analyzing substrate specificities of enzymes. It also explores other important uses of glycan microarrays, such as identifying ligands for lectins and functional glycans within cells.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Javier Ramos-Soriano, Javier Rojo
Summary: DC-SIGN, a lectin discovered two decades ago, plays a relevant role in innate immunity and has become a target for developing antiviral drugs due to its participation in the infection process of various pathogens. A variety of carbohydrate multivalent systems using different scaffolds have been described to achieve this goal, with significant contributions from our research group.
CHEMICAL COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Albertus Viljoen, Alain Vercellone, Myriam Chimen, Gerald Gaibelet, Serge Mazeres, Jerome Nigou, Yves F. Dufrene
Summary: The pathogenic bacterium Mycobacterium tuberculosis evades the immune system by binding to the C-type lectin DC-SIGN on dendritic cells. Our multidisciplinary study combining atomic force microscopy, Forster resonance energy transfer, and bioassays unravels the molecular mechanism behind the selective recognition of this receptor. We found that the distribution of DC-SIGN ligands differs between MyMTBC species, with dense nanodomains observed on M. bovis BCG. Upon bacteria-host cell adhesion, ligand nanodomains induce the recruitment and clustering of DC-SIGN, highlighting the importance of ligand clustering in pathogen recognition.
Article
Cell Biology
Yinan Zhang, Zhen Feng, Yue Xu, Sufen Jiang, Qianshi Zhang, Zhenyu Zhang, Keyong Wang, Xiaomeng Li, Lijie Xu, Menglang Yuan, Zihao Chen, Jingyi Cui, Han Wu, Yina Gao, Wei Wei, Bo Wang, Yunfei Zuo, Shuangyi Ren
Summary: LSECtin promotes lymphatic metastasis in gastric cancer by upregulating FN1 and CHD4 expression through the circFBXL4/miR-146a-5p/STAT1 axis.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Meina Gao, Hui Li, Chenghao Ye, Kaixian Chen, Hualiang Jiang, Kunqian Yu
Summary: The study identified the crucial mechanism of DC-SIGN binding to the SARS-CoV-2 Spike protein, providing a novel approach for exploring COVID-19 treatments, and predicted potential glycan epitopes. Additionally, molecular dynamics simulations revealed specific binding pockets for oligosaccharides.
Article
Biochemistry & Molecular Biology
Mike Aoun, Xiaojie Cai, Bingze Xu, Gonzalo Fernandez Lahore, Michael Yi Bonner, Yibo He, Liselotte Backdahl, Rikard Holmdahl
Summary: This study reveals the additive effect of Clec4b and Ncf1 on arthritis by regulating neutrophil function. It also shows that Clec4b-specific stimulation triggers neutrophils into oxidative burst. These findings highlight the importance of Clec4b as a regulator of neutrophils and demonstrate how epistatic interactions affect the susceptibility to, and severity of, autoimmune arthritis.
Article
Chemistry, Multidisciplinary
Xuefeng Cao, Shuaishuai Wang, Madhusudhan Reddy Gadi, Ding Liu, Peng G. Wang, Xiu-Feng Wan, Jian Zhang, Xi Chen, Lauren E. Pepi, Parastoo Azadi, Lei Li
Summary: This article describes the systematic synthesis of bisected N-glycans and their application in constructing a glycan microarray for studying their recognition by various glycan-binding proteins.
Article
Immunology
Tomonori Kaifu, Rikio Yabe, Takumi Maruhashi, Soo-Hyun Chung, Hiroaki Tateno, Noriyuki Fujikado, Jun Hirabayashi, Yoichiro Iwakura
Summary: The inhibitory C-type lectin receptor DCIR interacts with asialo-biantennary N-glycans to negatively regulate osteoclastogenesis and antigen presentation by dendritic cells, which has important implications for autoimmune and bone metabolic diseases.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Cell Biology
Naohiro Seo, Junko Nakamura, Tsuguhiro Kaneda, Hiroaki Tateno, Asako Shimoda, Takanori Ichiki, Koichi Furukawa, Jun Hirabayashi, Kazunari Akiyoshi, Hiroshi Shiku
Summary: The study proposes an effective method for preparing high-performance exosomes (EXOs) using an anion-exchange method. This method allows for the large-scale separation of bioactive EXOs and MV-like EVs, providing valuable insights for research on reliable bioactivity and drug discovery of extracellular vesicles.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Review
Cell Biology
Lalhaba Oinam, Hiroaki Tateno
Summary: Glycans are essential building blocks of life located on the outer surface of cells. A new technology called scGR-seq enables the simultaneous analysis of glycans and RNA in single cells, allowing for a deeper understanding of glycans' role in cellular communication. This technology has the potential to greatly advance glycobiology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Yu Yang, Yoshimasa Akashi, Osamu Shimomura, Hiroaki Tateno, Sayoko Saito, Keiko Hiemori, Yoshihiro Miyazaki, Tomoaki Furuta, Daichi Kitaguchi, Yukihito Kuroda, Louphrasitthiphol Pakavarin, Tatsuya Oda
Summary: Specific glycan profiles in SRC cells were reported, showing reduced alpha 2-6-linked sialic acids. Additionally, a targeted therapy using the rBC2LCN lectin might be applicable as an alternative treatment option for patients with SRC.
Article
Biology
Masamichi Nagae, Tetsuya Hirata, Hiroaki Tateno, Sushil K. Mishra, Noriyoshi Manabe, Naoko Osada, Yuko Tokoro, Yoshiki Yamaguchi, Robert J. Doerksen, Toshiyuki Shimizu, Yasuhiko Kizuka
Summary: X-ray crystallography, NMR, and computer modeling were used to investigate the structure and catalytic mechanism of GnTIVa, an enzyme involved in the synthesis of GlcNAc branches. The enzyme contains a C-terminal lectin domain that regulates its activity. The lectin domain was found to interact selectively with N-glycans having a beta 1-4 GlcNAc branch. Our findings provide insights into the biosynthesis of branching structures in N-glycans.
COMMUNICATIONS BIOLOGY
(2022)
Article
Urology & Nephrology
Masayuki Tasaki, Hiroaki Tateno, Takashi Sato, Hisashi Narimatsu, Kazuhide Saito, Yuki Nakagawa, Toshinari Aoki, Masami Kamimura, Takashi Ushiki, Kota Takahashi, Yoshihiko Tomita
Summary: The study investigated the reactivity of anti-ABO antibodies detected after ABO-incompatible kidney transplantation with stable graft function to the renal graft endothelial cells, and found that the antibody reactivity was low.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kazuhiko Yamasaki, Naruhiko Adachi, Mya Myat Ngwe Tun, Akihito Ikeda, Toshio Moriya, Masato Kawasaki, Tomoko Yamasaki, Tomomi Kubota, Izuru Nagashima, Hiroki Shimizu, Hiroaki Tateno, Kouichi Morita
Summary: The N-glycosylation sites on the spike protein of SARS-CoV-2 are relatively invariant and play a crucial role in infection. Core-fucosylated N-glycans have a high affinity for the spike protein and can inhibit viral infection across different variants. Cryogenic electron microscopy revealed that the multivalence of core-fucosylated N-glycans leads to the aggregation of the spike protein. This study highlights the potential for developing specific binders targeted at the spike protein.
Article
Chemistry, Multidisciplinary
Takahiro Kosaka, Satoshi Yamaguchi, Shin Izuta, Shinya Yamahira, Yoshikazu Shibasaki, Hiroaki Tateno, Akimitsu Okamoto
Summary: Methods to construct single-cell pairs of heterogeneous cells are important for analyzing intercellular communications and have potential in cell biological and medical applications. Photoactivatable substrate surfaces offer a promising tool for achieving single-cell pairing. By using a bioorthogonal photoreactive moiety and a non-cell adhesive hydrophilic polymer, we developed a photoresponsive material that enables light-induced anchoring of various cell types. This material-coated surface allows the construction of cancer cell and natural-killer (NK) cell pairs, facilitating the investigation of cell communication at a single-cell level.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Oncology
Yukihito Kuroda, Tatsuya Oda, Osamu Shimomura, Shinji Hashimoto, Yoshimasa Akashi, Yoshihiro Miyazaki, Kinji Furuya, Tomoaki Furuta, Hiromitsu Nakahashi, Pakavarin Louphrasitthiphol, Bryan J. Mathis, Takahito Nakajima, Hiroaki Tateno
Summary: Pancreatic ductal adenocarcinoma (PDAC) is resistant to current treatments but lectin-based phototherapy (Lec-PT) targeting cell surface glycans shows promise as a new therapy. The study combines a PDAC targeting lectin with a photoabsorber to create a near-infrared light-activated therapy. In vitro and in vivo experiments demonstrate cytotoxicity and tumor growth inhibition upon exposure to near-infrared light.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Yukihito Kuroda, Tatsuya Oda, Osamu Shimomura, Pakavarin Louphrasitthiphol, Bryan J. Mathis, Hiroaki Tateno, Kentaro Hatano
Summary: By targeting cell surface glycans, a novel class of tumor-specific probes for positron emission tomography (PET) has been developed to improve the early detection of pancreatic ductal adenocarcinoma (PDAC). The PDAC-targeting ability of [F-18]FB-rBC2LCN allows for high-contrast PET imaging of tumors in a mouse model, providing an accurate and sensitive tool for early-stage pancreatic cancer detection.
Article
Cell & Tissue Engineering
Tianwei Huang, Yuya Sato, Akiko Kuramochi, Yoshio Ohba, Masayuki Sano, Makoto Miyagishi, Hiroaki Tateno, Renu Wadhwa, Kazunori Kawasaki, Takeyuki Uchida, Kristina N. Ekdahl, Bo Nilsson, Ung-il Chung, Yuji Teramura
Summary: This study aimed to improve intracellular delivery to endothelial cells by modifying the exosome surface with Tat peptide (YGRKKRRQRRR)-PEG-lipids. Tat-conjugated PEG-lipids with different spacer molecular weights and lipid types were incorporated into exosomes using fluorescein isothiocyanate labeling to optimize the number of Tat-PEG-lipids immobilized on the exosome surface. The exosomes modified with Tat-PEG-lipids showed high detection inside human umbilical vein endothelial cells (HUVECs), indicating improved intracellular delivery.
REGENERATIVE THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Shuuji Mawaribuchi, Osamu Shimomura, Tatsuya Oda, Keiko Hiemori, Kayoko Shimizu, Kenya Yamase, Mutsuhiro Date, Hiroaki Tateno
Summary: Early detection for improving the survival of patients with pancreatic ductal adenocarcinoma (PDAC) is urgently needed. We identified SERPINA3 as a glycoprotein (BC2-S3) secreted from PDAC cells into the blood, which is reactive to rBC2LCN lectin. BC2-S3 exhibited higher levels in PDAC patients and showed a significantly higher detection rate for early stage PDAC than CA19-9.
Meeting Abstract
Oncology
Yukihito Kuroda, Osamu Shimomura, Hiromitsu Nakahashi, Yoshihiro Miyazaki, Takahito Nakajima, Hiroaki Tateno, Tatsuya Oda
Meeting Abstract
Urology & Nephrology
Masayuki Tasaki, Hiroaki Tateno, Takashi Sato, Azusa Tomioka, Hiroyuki Kaji, Hisashi Narimatsu, Kazuhide Saito, Toshinari Aoki, Masami Kamimura, Takashi Ushiki, Yutaka Yoshida, Kota Takahashi, Yoshihiko Tomita
JOURNAL OF UROLOGY
(2022)
Article
Surgery
Masayuki Tasaki, Hiroaki Tateno, Takashi Sato, Azusa Tomioka, Hiroyuki Kaji, Hisashi Narimatsu, Kazuhide Saito, Yuki Nakagawa, Toshinari Aoki, Masami Kamimura, Takashi Ushiki, Manabu Okada, Yuko Miwa, Kiyohiko Hotta, Yutaka Yoshida, Kota Takahashi, Yoshihiko Tomita
Summary: In this study, a new method to measure antibody titer using a microarray of recombinant CD31 linked to ABO antigens was developed. The results showed that this method had higher sensitivity and specificity in predicting antibody-mediated rejection in kidney transplantation compared to traditional isohemagglutinin assays.
TRANSPLANT INTERNATIONAL
(2022)
Meeting Abstract
Oncology
Yukihito Kuroda, Osamu Shimomura, Hiromitsu Nakahashi, Tomoaki Furuta, Yoshihiro Miyazaki, Takahito Nakajima, Hiroaki Tateno, Tatsuya Oda