Article
Microbiology
Shuo Yang, Junfeng Shen, Jiliang Deng, Hongxing Li, Jianzhi Zhao, Hongting Tang, Xiaoming Bao
Summary: This study focuses on improving the production of recombinant proteins in yeast cell factories by modifying cell polarization. Overexpression of genes related to cell polarization can enhance the surface display and secretion of recombinant proteins. Combinatorial modification of gene expression can increase protein production in a dose-dependent manner.
Article
Biotechnology & Applied Microbiology
Nanzhu Chen, Shuo Yang, Dawei You, Junfeng Shen, Banlai Ruan, Mei Wu, Jianzhi Zhang, Xiaozhou Luo, Hongting Tang
Summary: This study systematically investigated the effect of engineering cell wall biosynthesis on the activity of cellulolytic enzymes and found that the inactivation of certain genes significantly improved the secretion and surface-display of β-glucosidase. Furthermore, combinatorial modifications of these genes, along with the use of rich medium, greatly increased the activity of the enzymes. Proteomic analysis also revealed the involvement of translation processes in improving enzyme activity through cell wall biosynthesis engineering.
METABOLIC ENGINEERING
(2023)
Article
Biochemical Research Methods
Yubo Fang, Han Xiao
Summary: Efficient secretion of triterpenoids can be achieved by overexpressing YPS3 and SCW10 genes in engineered Saccharomyces cerevisiae strains, resulting in increased production of GA-HLDOA and changes in cell permeability and outer mannan layer.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Engineering, Chemical
Boitumelo F. Mashangoane, Evans N. Chirwa
Summary: Platinum group metals (PGMs) are elements that are concentrated in the earth's core and mantle, with low natural abundances. Due to their limited supply, there is economic value in recovering and reusing PGMs from waste solutions. Conventional methods for removing PGMs from wastewater have drawbacks, leading to a search for new technologies such as biosorption. This research project successfully developed a genetically modified strain of Saccharomyces cerevisiae EBY100 for biosorption of Pd (II) in aqueous solutions, with a maximum adsorption capacity of 125 mg/g.
MINERALS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Hong Le Linh, Nguyen Pham Anh Thu, Tran Thi Xuan Dung, Nguyen Van Hau, Nguyen Hieu Nghia, Dang Thi Phuong Thao
Summary: This study demonstrates a new successful strategy of using yeast cell surface to produce a VP28-based oral vaccine for shrimp aquaculture, leading to improved survival rates and enhanced efficiency of probiotic strains for shrimp farming.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Samuel R. Plante, Kyung-Mee Moon, Pascale Lemieux, Leonard Foster, Christian Landry
Summary: Spores of Saccharomyces cerevisiae have a highly viscous and acidic cytosol, which alters the solubility of proteins and is essential for their germination and growth. The heat shock protein, Hsp42, plays a crucial role in this process by facilitating protein dissolution.
Review
Biotechnology & Applied Microbiology
Chenmeng Zhang, Hongyu Chen, Yiping Zhu, Yu Zhang, Xun Li, Fei Wang
Summary: Microbial cell surface display technology is a powerful platform for engineering proteins/peptides with improved properties. Saccharomyces cerevisiae is an ideal cell factory for this technology due to its large cell size, Generally Regarded As Safe (GRAS) status, and post-translational processing capabilities. This technology has various applications in industrial processes, including biofuels and chemical production, environmental pollution treatment, and immunization processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mi-Jin Kim, Bong Hyun Sung, Hyun-Jin Kim, Jung-Hoon Sohn, Jung-Hoon Bae
Summary: Protein expression with a fusion partner followed by the removal of the fusion partner via in vitro processing with a specific endoprotease is a favored method for efficient production of intact recombinant proteins. This study developed an autolysis-proof Kex2p endoprotease, facilitating the large-scale production of recombinant proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Pavani Vamsi Krishna Nittala, Allison Hohreiter, Emilio Rosas Linhard, Ryan Dohn, Suryakant Mishra, Abhiteja Konda, Ralu Divan, Supratik Guha, Anindita Basu
Summary: The paper presents fabrication methodologies that integrate silicon components into soft microfluidic devices for cell lysis. The integration methodology involves a silicon chip with microstructure arrays embedded in a microfluidic device, which is actuated by piezoelectric force to physically break microbial cell walls. Different silicon microarray geometries, fabrication techniques, integration methods, and efficacy evaluation using synthetic microbeads and yeast species are presented. The proposed integration methodology can serve as an important process step for future hybrid silicon-polymeric devices in cellular processing applications.
Article
Biotechnology & Applied Microbiology
Pamela B. Besada-Lombana, Wilfred Chen, Nancy A. Da Silva
Summary: The study implemented a GPCR-based extracellular glucose sensor to enable enzyme expression and secretion based on extracellular glucose levels. By modifying the promoter and optimizing the display system, the expression and cell-surface display of a beta-glucosidase were achieved, with an increase in enzymatic activity when cellobiose was added.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin, Jung-Hoon Sohn
Summary: The extracellular conversion of xylose to xylulose before uptake has been found to be effective in xylose fermentation or glucose/xylose co-fermentation, reducing the competition between glucose and xylose for cellular uptake. The engineered XI secretion system developed in this study may enable the simultaneous utilization of C5/C6 sugars from sustainable lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Yeping Lin, Yunzi Feng, Lin Zheng, Mouming Zhao, Mingtao Huang
Summary: This study focuses on the yeast Saccharomyces cerevisiae and its potential for increased protein production through genetic engineering. By targeting genes related to protein synthesis and using transcriptome analysis and physiological characterizations, key regulatory and metabolic network changes were identified. It was found that manipulating genes relevant to stress response in protein synthesis significantly increased protein secretion. The genetic modifications identified in this study provide valuable targets for constructing efficient yeast cell factories for protein production.
METABOLIC ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Quanli Liu, Yi Liu, Gang Li, Otto Savolainen, Yun Chen, Jens Nielsen
Summary: Isoflavonoids are industrially important plant natural products, but their low abundance and structural complexity limit their availability. By engineering Saccharomyces cerevisiae metabolism to efficiently produce daidzein, a core chemical scaffold for isoflavonoid biosynthesis, researchers demonstrate its application for producing bioactive glucosides from glucose.
NATURE COMMUNICATIONS
(2021)
Article
Food Science & Technology
Yue Gu, Jianjun Tian, Yue Zhang, Jing Wu, Yinfeng He
Summary: This study explored the effects of Saccharomyces cerevisiae cell-free supernatant (CFS) on lactic acid bacteria (LAB), and found that the CFS inhibited LAB growth, acid production, biofilm formation, and influenced the LAB's quorum sensing and protein expression.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Shou-Chen Lo, Chia-Yin Yang, Dony Chacko Mathew, Chieh-Chen Huang
Summary: The study found that Kluyveromyces marxianus grown with lactate as the sole carbon source showed optimal growth and released protein under different pH conditions. Lactate induced the autolysis of K. marxianus, leading to the release of proteins and peptides. These findings are crucial for future probiotic and kefir studies involving K. marxianus.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Clara Bouyx, Marion Schiavone, Marie-Ange Teste, Etienne Dague, Nathalie Sieczkowski, Anne Julien, Jean Marie Francois
Summary: The study reveals that flocculins encoded by FLO11 in Saccharomyces cerevisiae behave like adhesins in Candida albicans, and the presence of amyloid-forming sequences is crucial for nanodomain formation in the Flo11 protein.
Article
Plant Sciences
Anis Meschichi, Mathieu Ingouff, Claire Picart, Marie Mirouze, Sophie Desset, Franck Gallardo, Kerstin Bystricky, Nathalie Picault, Stefanie Rosa, Frederic Pontvianne
Summary: This study utilized the ANCHOR system to track gene positioning and chromatin mobility in plant cells, providing a novel approach for understanding the role of genes in plant cells.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jingjing Li, Dongge Xu, Lingling Wang, Mengyu Zhang, Guohai Zhang, Erguang Li, Susu He
Summary: Glycyrrhizic acid exhibits antiviral activity against SARS-CoV-2 by targeting the virus for infection, as shown through inhibition of S protein binding and cell entry. The study also suggests that the antiviral effect of glycyrrhizic acid is linked to its impact on virus cell binding.
Article
Multidisciplinary Sciences
Guilherme M. Oliveira, Attila Oravecz, Dominique Kobi, Manon Maroquenne, Kerstin Bystricky, Tom Sexton, Nacho Molina
Summary: The study introduces a new computational method, GP-FBM, to extract chromatin diffusion parameters and shows that there is no significant difference in diffusion properties between mitotic and interphase chromatin in mouse embryonic stem cells. The research demonstrates heterogeneity in local chromatin dynamics and includes the development of a user-friendly graphical interface, GP-Tool, for easier utilization by the research community.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Lingling Wang, Dongge Xu, Qun Huang, Guang Yang, Mengyu Zhang, Jingai Bi, Jinjun Shan, Erguang Li, Susu He
Summary: The study revealed a potential interplay between tonsil microbiota and HAdV reactivation, showing that different distribution patterns of microorganisms in adenoviral DNA-positive and -negative samples might contribute to the reactivation of the virus. Short-chain fatty acids, primary metabolites of microbiota on tonsils, were found to induce HAdV reactivation in tonsil lymphocytes. This viral-bacterial interaction may lead to recurrent viral infections in pediatric patients.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Ziyi Liu, Zhiqiang Wang, Xiaoyu Lu, Kai Peng, Sheng Chen, Susu He, Ruichao Li
Summary: The study found that cointegrate plasmids are structurally unstable and may undergo fragment deletion and gene insertion during transmission. These plasmids impose a fitness cost on bacterial hosts and enhance biofilm formation ability, but they may exhibit higher stability in antibiotic environments.
Review
Microbiology
Clara Bouyx, Marion Schiavone, Jean Marie Francois
Summary: The yeast Saccharomyces cerevisiae demonstrates remarkable adaptability to fluctuating or hostile environments through specialized surface proteins called flocculins, with FLO11 encoding a unique flocculin variant characterized by distinct domain sequence and genetic regulation. This plasticity allows yeast cells to swiftly adapt to new ecological niches and hostile environments, making FLO11 a valuable model for studying cell adhesion and biofilm formation mechanisms.
Article
Microbiology
Melissa Tan, Yanis Caro, Alain Shum Cheong Sing, Heloise Reiss, Jean-Marie Francois, Thomas Petit
Summary: Yeast volatile organic compounds (VOCs) have potential as natural aromas and can replace artificial flavors, with unconventional yeast strains showing promise in producing alternative VOCs. A Saprochaete suaveolens strain isolated from dragon fruit in Reunion Island demonstrated enhanced production of alpha-unsaturated esters through UV mutagenesis, marking a significant advancement in VOC enhancement in microbial strains.
Article
Biochemistry & Molecular Biology
Dan Zhang, Yuanhui Zhao, Lingling Wang, Xiaoxin You, Jingjing Li, Guohai Zhang, Yayi Hou, Hongwei Wang, Susu He, Erguang Li
Summary: This study found that Axl acts as a suppressor of the antiviral response during RSV infection by negatively regulating IFN signaling.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Ludmila Recoules, Alexandre Heurteau, Flavien Raynal, Nezih Karasu, Fatima Moutahir, Fabienne Bejjani, Isabelle Jariel-Encontre, Olivier Cuvier, Thomas Sexton, Anne-Claire Lavigne, Kerstin Bystricky
Summary: The histone variant macroH2A1.1 plays a role in cancer development and metastasis by selectively binding to active promoters and enhancers, as well as facultative heterochromatin. Its knock down results in the deregulation of expression of hundreds of highly active genes. MacroH2A1.1 acts through two distinct molecular mechanisms, mitigating excessive transcription and stimulating expression of RNA polymerase II-paused genes. It serves as a transcriptional modulator and potentially assists the conversion of promoter-locked Pol II into a productive, elongating Pol II.
JOURNAL OF CELL SCIENCE
(2022)
Article
Genetics & Heredity
Clotilde laussel, Veronique Albanese, Francisco Javier Garcia-Rodriguez, Alberto Ballin, Quentin Defenouillere, Sebastien Leon
Summary: 2-deoxyglucose triggers endocytosis of plasma membrane proteins in a Rod1-dependent manner, with Rod1 stabilizing glucose transporters at the plasma membrane to facilitate glucose uptake and counteract the metabolic blockade caused by 2DG.
Article
Microbiology
Ceren Alkim, Daniele Farias, Julie Fredonnet, Helene Serrano-Bataille, Pauline Herviou, Marc Picot, Nawel Slama, Sebastien Dejean, Nicolas Morin, Brice Enjalbert, Jean M. Francois
Summary: L-homoserine plays a crucial role in the carbon and nitrogen metabolism of E. coli, but it cannot be used as a nitrogen source for growth. Through laboratory evolution, a strain was obtained that could grow with L-homoserine as a nitrogen source and was not sensitive to its growth inhibition. Sequencing revealed only a few genomic modifications in the evolved strain, providing insights into the mechanisms of L-homoserine's dual effects and toxicity in E. coli.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Dan Zhang, Yuanhui Zhao, Xiaoxin You, Susu He, Erguang Li
Summary: RSV infection is a common pulmonary infection in infants and the elderly, but no specific therapeutics are available. In this study, Axl kinase inhibitors were found to reduce RSV infection and promote the expression of antiviral genes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Mengyu Zhang, Weikang Sun, Xiaoxin You, Dongge Xu, Lingling Wang, Jingping Yang, Erguang Li, Susu He
Summary: This study analyzed publicly available datasets and found that EBV-associated gastric cancer (EBVaGC) has a repression of L1 transcription compared with EBV-negative gastric cancer (EBVnGC). Retrotransposition-associated young and full-length L1s (FL-L1s) were found to be the most repressed L1s in EBVaGC. Increased deposition of H3K9me3 on FL-L1s was observed, potentially attributed to increased TASOR expression and the interaction between viral DNA and the TASOR enhancer. This study uncovers a regulation mechanism of L1 expression by chromatin topology remodeling associated with viral-host genome interaction in EBVaGC.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Jean Marie Francois
Summary: Methionine, an essential sulphur-containing amino acid, is predominantly produced chemically from fossil resources, causing environmental problems and potential oil scarcity. This review discusses the current state of biobased alternatives for sustainable production of methionine using renewable carbon feedstock. Three bio-based processes, including purely fermentative process, mixed process, and hybrid process, are highlighted. The industrial potentials and remaining technical and economic challenges of these bioprocesses are extensively examined. The review concludes by emphasizing the benefits of these bioprocesses in achieving the green transition and enhancing the local resources in the model of Bioeconomy.
BIOTECHNOLOGY ADVANCES
(2023)