Article
Biochemistry & Molecular Biology
Jingwen Li, Jingfei Chen, Yefei Wang, Lishan Yao
Summary: The hydrogen bond cooperativity in the beta-sheet of GB3 protein is investigated using a NMR hydrogen/deuterium exchange method. It is found that weakening one hydrogen bond perturbs other neighboring hydrogen bonds, with three being cooperative and one being anticooperative. This hydrogen bond cooperativity plays a significant role in the stability and conformational coupling of the beta-sheet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yuichi Sato, Mako Takiguchi, Haruna Tamano, Atsushi Takeda
Summary: The extracellular dynamics of Zn2+ in the hippocampus is influenced by glutamatergic synapse excitation and the presence of the Alzheimer's disease causative peptide Aβ(1-42), leading to cognitive decline. Aging accelerates Aβ(1-42)-induced intracellular Zn2+ toxicity due to an increase in extracellular Zn2+. Metallothioneins can serve as buffers for intracellular Zn2+ dysregulation caused by Aβ(1-42).
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Engineering, Environmental
Edyta Nartowska, Tomasz Kozlowski, Marta Kolankowska
Summary: The study found that the type of exchangeable cations significantly affects the unfrozen water content in montmorillonite, and proposed empirical models that can predict the unfrozen water content after ion exchange.
COLD REGIONS SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Biomedical
Bibin Anand, Qi Wu, Maryam Nakhaei-Nejad, Govindarajan Karthivashan, Lyudmyla Dorosh, Sara Amidian, Abhishek Dahal, Xiuju Li, Maria Stepanova, Holger Wille, Fabrizio Giuliani, Satyabrata Kar
Summary: Native PLGA nanoparticles show therapeutic potential in the treatment of Alzheimer's disease by suppressing aggregation of beta-amyloid peptides, triggering their disassembly, reducing phosphorylation of tau protein, enhancing neuronal viability, and attenuating memory deficits and A beta levels in animal models of AD.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Vivian Lorena Leguizamon Herrera, Alexander K. Buell, Dieter Willbold, Bogdan Barz
Summary: The study elucidates the structural interaction and thermodynamics of binding between three D-peptides and A beta 42 monomers, revealing that the binding is mainly driven by electrostatic and hydrophobic interactions and leads to more compact conformations.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xiang Zhou, Yuanyuan Wang, Wei Zheng, Guangxiu Deng, Fuyi Wang, Lan Jin
Summary: The aggregation of beta-amyloid peptide (Aβ) is one potential cause for Alzheimer's disease, and heparin can either promote or inhibit Aβ aggregation. The sulfation pattern and chain size of heparin determine its binding affinity to Aβ. By studying the glycosaminoglycan (GAG)-peptide complex and conducting an MTT assay, it was found that heparin oligosaccharides with defined sequences represent promising inhibitors of Aβ aggregation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Santiago Ramirez, Suelyn Koerich, Natalia Astudillo, Nicole De Gregorio, Rabab Al-Lahham, Tyler Allison, Natalia Pessoa Rocha, Fei Wang, Claudio Soto
Summary: This study aimed to evaluate the effect of removing Aβ from blood plasma on the accumulation of amyloid plaques in the brain. The results showed a reduction in Aβ levels in the plasma and insoluble brain fractions, as well as a decrease in amyloid plaque burden and changes in plaque size distribution in the cortex and hippocampus. These findings support the importance of targeting Aβ in the periphery and suggest plasma exchange as a potential non-pharmacological strategy for slowing down AD pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Zi-Xuan Tan, Fang Dong, Lin-Yu Wu, Ya-Shuo Feng, Feng Zhang
Summary: Alzheimer's disease is a major burden on global healthcare systems, and effective treatments are needed. Accumulation of neurotoxic beta-amyloid peptide (A beta) is a feature of AD, but medications targeting its reduction have yet to prove effective. Physical exercise has benefits for AD, including reducing A beta deposition to alleviate symptoms.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Angelo Santoro, Manuela Grimaldi, Michela Buonocore, Ilaria Stillitano, Anna Maria D'Ursi
Summary: This study utilized NMR to analyze the conformational transition of A beta(1-42) in 50/50 HFIP/water, revealing unexpected routes in the evolution from helical to beta-sheet structures. Molecular dynamics simulations confirmed that the structural model calculated in this study is a starting point for amyloid fibrils formation.
Article
Biochemistry & Molecular Biology
Hongtao Du, Jinzhi Song, Fang Ma, Hongxin Gao, Xinyan Zhao, Renjun Mao, Xiaolong He, Yan Yan
Summary: This study synthesised and characterised a series of novel harmine derivatives as potential ligands for AD treatment. Compounds 13 and 17d showed exceptional neuroprotective effects and potent inhibition against AChE and A beta aggregation. Kinetic studies and molecular modelling confirmed the interaction of compound 13 with both CAS and PAS of AChE.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Shanshan Zhang, Sajid Asghar, Chenqi Zhu, Junxiu Ye, Ling Lin, Liu Xu, Ziyi Hu, Zhipeng Chen, Feng Shao, Yanyu Xiao
Summary: This study presents a multi-functional delivery system (APND-3) designed to address the issues of poor drug delivery to the brain in Alzheimer's disease (AD) treatment. The system, based on a novel peptide (MOP) with self-assembling properties, significantly enhances the delivery of MOP to the brain, reducing A beta deposition and improving neurological outcomes in AD model mice.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Clinical Neurology
Shuta Ozawa, Yukiko Hori, Yusuke Shimizu, Atsuhiko Taniguchi, Takanobu Suzuki, Wenbo Wang, Yung Wen Chiu, Reiko Koike, Satoshi Yokoshima, Tohru Fukuyama, Sho Takatori, Youhei Sohma, Motomu Kanai, Taisuke Tomita
Summary: The study demonstrated that photo-oxygenation facilitates the clearance of aggregated amyloid-beta from the brains of living Alzheimer's disease model mice, and enhances the microglial degradation of amyloid-beta peptide, suggesting a novel strategy for Alzheimer's disease treatment that may be compatible with immunotherapy.
Article
Pharmacology & Pharmacy
Jing Liu, Ye Lin, Yang Yang, Yane Guo, Yanchang Shang, Bo Zhou, Tianlong Liu, Jiao Fan, Chao Wei
Summary: This study demonstrates that chronic Z-GS treatment can improve cognitive and neuropathological impairments in transgenic mouse models of Alzheimer's disease (AD). Z-GS treatment can improve learning and memory deficits, reduce amyloid-beta levels and plaque burden, alleviate neuroinflammation and synaptic defects. Furthermore, Z-GS treatment inhibits the TLR4/NF-kappa B signaling pathway and regulates amyloid precursor protein processing.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bartlomiej Stanczykiewicz, Tomasz M. Goszczynski, Pawel Migdal, Marta Piksa, Krzysztof Pawlik, Jakub Gburek, Krzysztof Golab, Boguslawa Konopska, Agnieszka Zablocka
Summary: The research aims to determine the inhibitory activity of ovocystatin, a cysteine protease inhibitor isolated from egg white, on A beta 42 fibril formation in vitro. The results show that ovocystatin possesses A beta 42 anti-aggregation activity and inhibits A beta 42 oligomer toxicity in PC12 cells. This research may contribute to the development of potential substances to prevent or delay beta-amyloid aggregation, a major cause of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyu Zhang, Xiancheng Zhang, Manli Zhong, Pu Zhao, Chuang Guo, You Li, He Xu, Tao Wang, Huiling Gao
Summary: A novel Cu(II)-binding peptide identified through phage display technology showed potential in reducing Cu-induced Aβ aggregation by regulating metal ion distribution and inhibiting Aβ production through suppressing BACE1 expression and improving cell oxidative damage. This peptide also demonstrated low toxicity in cell culture experiments, suggesting a potential therapeutic approach to prevent or treat AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Jin Zhang, Afshan Begum, Kristoffer Brannstrom, Christin Grundstrom, Irina Iakovleva, Anders Olofsson, A. Elisabeth Sauer-Eriksson, Patrik L. Andersson
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2016)
Article
Multidisciplinary Sciences
Michael Hall, Christin Grundstrom, Afshan Begum, Mikael J. Lindberg, Uwe H. Sauer, Fredrik Almqvist, Jorgen Johansson, A. Elisabeth Sauer-Eriksson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Michael Kovermann, Christin Grundstrom, A. Elisabeth Sauer-Eriksson, Uwe H. Sauer, Magnus Wolf-Watz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Chemistry, Medicinal
Martina Kulen, Marie Lindgren, Sabine Hansen, Andrew G. Cairns, Christin Grundstrom, Afshan Begum, Ingeborg van der Lingen, Kristoffer Brannstrom, Michael Hall, Uwe H. Sauer, Jorgen Johansson, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Engineering, Environmental
Jin Zhang, Christin Grundstrom, Kristoffer Brannstrom, Irina Iakovleva, Mikael Lindberg, Anders Olofsson, Patrik L. Andersson, A. Elisabeth Sauer-Eriksson
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Irina Iakovleva, Afshan Begum, Kristoffer Brannstrom, Alexandra Wijsekera, Lina Nilsson, Jin Zhang, Patrik L. Andersson, A. Elisabeth Sauer-Eriksson, Anders Olofsson
Article
Multidisciplinary Sciences
Lina Nilsson, Andreas Larsson, Afshan Begum, Irina Iakovleva, Marcus Carlsson, Kristoffer Brannstrom, A. Elisabeth Sauer-Eriksson, Anders Olofsson
Article
Cell Biology
Emilia Krypotou, Mariela Scortti, Christin Grundstrom, Melanie Oelker, Ben F. Luisi, A. Elisabeth Sauer-Eriksson, Jose Vazquez-Boland
Article
Biochemistry & Molecular Biology
Per Rogne, David Andersson, Christin Grundstrom, Elisabeth Sauer-Eriksson, Anna Linusson, Magnus Wolf-Watz
Article
Microbiology
Sabine Hansen, Michael Hall, Christin Grundstrom, Kristoffer Brannstrom, A. Elisabeth Sauer-Eriksson, Jorgen Johansson
JOURNAL OF BACTERIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Per Rogne, Beata Dulko-Smith, Jack Goodman, Marie Rosselin, Christin Grundstrom, Christian Hedberg, Kwangho Nam, A. Elisabeth Sauer-Eriksson, Magnus Wolf-Watz
Article
Multidisciplinary Sciences
Irina Iakovleva, Michael Hall, Melanie Oelker, Linda Sandblad, Intissar Anan, A. Elisabeth Sauer-Eriksson
Summary: The study used cryo-EM to analyze the structure of vitreous body ATTR fibrils from an ATTR patient's eye, revealing differences from previously studied heart fibrils with the same mutation, indicating variations in ATTR protein structure and assembly in type A fibrils among patients with the same mutation. By analyzing amino acid interfaces, a mechanism for the structural conversion of TTR into fibrillar form was proposed.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Thao Thanh Tran, Carmen D. Mathmann, Marcela Gatica-Andrades, Rachel F. Rollo, Melanie Oelker, Johanna K. Ljungberg, Tam T. K. Nguyen, Alina Zamoshnikova, Lalith K. Kummari, Orry J. K. Wyer, Katharine M. Irvine, Javier Melo-Bolivar, Annette Gross, Darren Brown, Jeffrey Y. W. Mak, David P. Fairlie, Karl A. Hansford, Matthew A. Cooper, Rabina Giri, Veronika Schreiber, Shannon R. Joseph, Fiona Simpson, Timothy C. Barnett, Joergen Johansson, Wendy Dankers, James Harris, Timothy J. Wells, Ronan Kapetanovic, Matthew J. Sweet, Eleanor A. Latomanski, Hayley J. Newton, Romain J. R. Guerillot, Abderrahman Hachani, Timothy P. Stinear, Sze Ying Ong, Yogeswari Chandran, Elizabeth L. Hartland, Bostjan Kobe, Jennifer L. Stow, A. Elisabeth Sauer-Eriksson, Jakob Begun, Jessica C. Kling, Antje Blumenthal
Summary: Listeria monocytogenes is a pathogen that escapes from its entry vacuole to replicate and spread in host cells. In this study, the inhibition of a key regulator of Listeria virulence resulted in the trapping of bacteria inside their entry vacuoles and extensive replication in spacious vacuoles. However, the majority of infected host immune cells were able to clear the bacteria, indicating that intra-vacuolar existence is not necessarily tied to bacterial persistence.
Article
Multidisciplinary Sciences
Apoorv Verma, Emma Aberg-Zingmark, Tobias Sparrman, Ameeq Ul Mushtaq, Per Rogne, Christin Grundstrom, Ronnie Berntsson, Uwe H. Sauer, Lars Backman, Kwangho Nam, Elisabeth Sauer-Eriksson, Magnus Wolf-Watz
Summary: Enzymatic catalysis relies on selectivity, active site architecture, and dynamics, which were investigated in this study using NMR, crystallography, and MD simulations on the phosphotransferase adenylate kinase (AK) from Odinarchaeota (OdinAK). OdinAK, a hyperthermophilic trimer, was found to use all NTPs for phosphorylation reaction, unlike other AK family members. Crystallographic structures revealed a common NTP-binding motif, while 19F NMR experiments showed a conserved and rate-limiting dynamic signature. The trimeric form of OdinAK lacked a critical catalytic residue in its active site, making it atypical. The findings provide insights into the evolution of enzymatic substrate specificity and cold adaptation through comparisons with human monomeric homologs.
Article
Biochemistry & Molecular Biology
Sonja Tischlik, Melanie Oelker, Per Rogne, A. Elisabeth Sauer-Eriksson, Malte Drescher, Magnus Wolf-Watz
Summary: This article investigates the role of adenylate kinases in cellular energy homeostasis and their interaction with diadenosine tetraphosphate (AP4A). It reveals that adenylate kinase dynamically interconverts between open and closed states in the presence of AP4A and has hydrolytic activity towards AP4A.