Article
Biochemical Research Methods
Diego Cuerda-Gil, Yu-Hung Hung, Kaushik Panda, R. Keith Slotkin
Summary: The sorting of RNA transcripts into different fates is mediated by their interaction with RNA-binding proteins, but the mechanism by which hundreds of RNA-binding proteins sort RNAs into distinct pathways is largely unknown. In this study, the researchers developed a protein-RNA tethering system that can be used in plants to engineer the fate of RNAs, determine the function of proteins when recruited to an RNA, and discover new interactions with RNA-binding proteins.
Article
Biochemistry & Molecular Biology
Vanya D. Barzilova, Josephine Drury, Bryony Rogers, Emily Thomas, Fareen Ahmed, Alice Bradfield, Hannan Al-Lamee, Dharani K. Hapangama
Summary: This study assessed the expression of nucleolin (NCL) in endometrial cancer (EC) and found that it is associated with tumor grading and prognosis. The study also identified dysregulated NCL-associated genes in EC. These findings suggest that NCL could be a potential target for diagnosis, risk stratification, and management of EC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Brisamar Estebanez, David Jimenez-Pavon, Chun-Jung Huang, Maria J. Cuevas, Javier Gonzalez-Gallego
Summary: This review systematically investigates the effects of exercise on the release and cargo of exosomes, showing that exercise promotes exosome release without modifying their size and leads to an increase in specific exosome markers. Various exosome-carried microRNAs involved in regulating skeletal muscle tissue proliferation and differentiation are released in response to exercise. However, methodological factors such as exosome isolation and characterization, as well as timing of sample recovery post-exercise, can impact the analysis and interpretation of findings.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jani Silva, Valeria Tavares, Ana Afonso, Juliana Garcia, Fatima Cerqueira, Rui Medeiros
Summary: Prostate cancer (PCa) is a common malignancy and advanced PCa leads to metastatic castration-resistant prostate cancer (mCRPC). This study evaluated the prognostic potential of miRNAs in liquid biopsies of mCRPC patients treated with ARAT agents. Low expression levels of miR-16-5p and miR-145-5p were associated with lower PFS in patients treated with abiraterone acetate. Low miR-20a-5p levels were associated with worse OS in patients with Gleason scores of <8.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Mahta Moraghebi, Reza Maleki, Mohsen Ahmadi, Ahmad Agha Negahi, Hossein Abbasi, Pegah Mousavi
Summary: The study found that SNPs in microRNA affect both miRNA function and miRNA expression, expanding molecular insight into how SNPs in different parts of miRNA, including the regulatory (promoter), the precursor (pre-miRNA), functional regions (seed region of mature miRNA), and RBP-binding motifs, may be correlated to Alzheimer's disease.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Joana M. O. Santos, Sara Peixoto da Silva, Margarida M. S. M. Bastos, Paula A. Oliveira, Rui M. Gil da Costa, Rui Medeiros
Summary: This study found that miR-223-3p, let-7b-5p, miR-21a-5p, miR-150-5p, and miR-155-5p are involved in muscle wasting in cancer cachexia, possibly through regulating the MAPK cascades.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Fouzieh Salimi, Gholamreza Asadikaram, Moslem Abolhassani, Yasin Pourfarjam, Hamid Zeynali Nejad, Mojtaba Abbasi-Jorjandi, Mojgan Sanjari
Summary: This study aimed to investigate the role of organochlorine pesticides (OCPs) in oxidative stress (OS) and the development of thyroid tumors. The results showed decreased enzyme activities and total antioxidant capacity, as well as increased levels of oxidative stress biomarkers in patients with thyroid tumors. Therefore, OCPs may contribute to the development of thyroid tumors through the generation of oxidative stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Khalid Zaman, Fazal Rahim, Muhammad Taha, Muhammad Sajid, Shawkat Hayat, Muhammad Nawaz, Mohammed Salahuddin, Naveed Iqbal, Naqeeb Ullah Khan, Syed Adnan Ali Shah, Rai Khalid Farooq, Ali Bahadar, Abdul Wadood, Khalid Mohammed Khan
Summary: This study synthesized quinoline analogs as urease inhibitors, which showed greater potency compared to the standard drug, thiourea. Some analogs were further tested for their cytotoxicity against C. elegans, with results showing minimal cytotoxic effects at higher concentrations. Protein-ligand binding interactions for the most potent analogs were confirmed through molecular docking study.
BIOORGANIC CHEMISTRY
(2021)
Review
Gastroenterology & Hepatology
Barbora Waclawikova, Agnese Codutti, Karen Alim, Sahar El Aidy
Summary: The human gastrointestinal tract is populated by trillions of microbes that play a significant role in regulating intestinal physiology, including gut motility. Understanding the inter-regulatory mechanisms between the microbiota and bowel function is crucial for addressing gut dysmotility and associated diseases.
Article
Biochemistry & Molecular Biology
Fernando Calzada, Elihu Bautista, Sergio Hidalgo-Figueroa, Normand Garcia-Hernandez, Elizabeth Barbosa, Claudia Velazquez, Rosa Maria Ordonez-Razo, Angel Giovanni Arietta-Garcia
Summary: The study demonstrated that IA exhibits high antilymphoma activity and lethality through BSL, with strong interactions in molecular docking studies. Additionally, IA showed low acute toxicity in mice.
Article
Optics
Marlon Rodrigues Garcia, Victor Sanchez, Thereza Cury Fortunato, Michelle Barreto Requena, Clovis Grecco, Jose Dirceu Vollet-Filho, Layla Pires, Lilian Tan Moriyama, Sebastiao Pratavieira
Summary: Photodynamic therapy is an effective treatment for various lesions. This study compared a theoretical model with a rat liver model to estimate the necrosis depths, volumes, and the concentrations of photosensitizers, oxygen, and reactive oxygen species (ROS) in the treated area. The study found that the ROS concentration threshold for Photogem in rat liver is about 1 mM. This proposed method can be used to estimate parameters for any photosensitizer or tissue, including layered tissues.
Article
Biochemistry & Molecular Biology
Sukhleen Kour, Tyler Fortuna, Eric N. Anderson, Darilang Mawrie, Jessica Bilstein, Ramakrishnan Sivasubramanian, Caroline Ward, Rishit Roy, Dhivyaa Rajasundaram, Jared Sterneckert, Udai Bhan Pandey
Summary: This research discovers that Drosha and two Drosha-dependent microRNAs have regulatory effects on the expression of FUS and prevent its cytoplasmic segregation. Depletion of Drosha can alleviate FUS-mediated degeneration and motor defects. miR-378i and miR-6832-5p differentially regulate the expression, solubility, and cytoplasmic aggregation of FUS.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nourhan Hisham Shady, Heba Ali Hassan, Mahmoud A. Elrehany, Mohamed Salah Kamel, Entesar Ali Saber, Sherif A. Maher, Fatma A. Abo-Elsoud, Ahmed M. Sayed, Usama Ramadan Abdelmohsen, Shereen S. Gaber
Summary: The study showed that the aqueous extract of Hyphaene thebaica fruits significantly reduced blood glucose levels, increased insulin expression, and decreased inflammatory mediator expression in diabetic rats. Histopathological examination revealed that the aqueous extract reversed beta-cell necrosis and restored its normal morphology.
Article
Biochemistry & Molecular Biology
Akira Kubota, Masashi Hirano, Yuka Yoshinouchi, Xing Chen, Michiko Nakamura, Yumi Wakayama, Jae Seung Lee, Haruhiko Nakata, Hisato Iwata, Yusuke K. Kawai
Summary: This study evaluated the estrogen-like effects of bisphenol A (BPA) and its analogs (BPs) using zebrafish. Most BPs concentration-dependently induced the expression of CYP19A1b, which was suppressed by a nonselective estrogen receptor antagonist. The EC50 values and relative potencies of BPs in inducing CYP19A1b expression were estimated, and some BPs showed greater estrogenic potency than BPA. Molecular docking simulations revealed lower interaction energies of certain BPs with zebrafish estrogen receptor subtypes compared to BPA, which correlated with their in vivo potencies.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Chemistry, Physical
Kirti Singh, Virendra Bahadur Yadav, Umakant Yadav, Gopal Nath, Anchal Srivastava, Paolo Zamboni, Pranali Kerkar, Preeti Suman Saxena, Ajay Vikram Singh
Summary: This study synthesized silver nanoparticles using green synthesis and developed a silver nanoparticle acticoat by incorporating them into carrageenan for wound healing. The acticoat showed excellent antibacterial properties and promoted wound healing and collagen deposition. The in vivo studies demonstrated its potential application in burn, accidental, and diabetic wound infections.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)