期刊
FEBS JOURNAL
卷 275, 期 22, 页码 5657-5666出版社
WILEY
DOI: 10.1111/j.1742-4658.2008.06691.x
关键词
acoustic standing wave forces; antigen-specific binding; microfluidic chip; phage display; selection
资金
- BioInvent International AB
- Swedish Research Council
- Crafoord Foundation
- Carl Trygger Foundation,
- Create Health
- Royal Physiographic Society
- ELFA Foundation
Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders.
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