Article
Agronomy
Feng-Yan Zhou, Heping Han, Yun-Jing Han, Alex Nyporko, Qin Yu, Hugh J. J. Beckie, Stephen B. B. Powles
Summary: The expression of AKR genes (LrAKR4C10 and LrAKR1) in glyphosate-resistant populations of Lolium rigidum from Western Australia is induced by glyphosate and 2,4-D, resulting in enhanced glyphosate metabolism and moderate resistance to glyphosate.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Kohji Yamamoto, Satoshi Endo
Summary: This study characterized a new AKR, AKR2E8, in silkworms, which showed similar properties to human AKR1B1 and AKR1B10 and was found to play a major role in aldehyde detoxification in the midgut. The findings may aid in developing pest control methods for lepidopterans, such as silkworms, that damage crops.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Food Science & Technology
Yogita Lugani, Munish Puri, Balwinder Singh Sooch
Summary: Xylose reductase is an industrially important food enzyme that plays a crucial role in converting xylose into xylitol, with diverse applications in various industries.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Microbiology
Dorthe Rippert, Federica Linguardo, Andreea Perpelea, Mathias Klein, Elke Nevoigt
Summary: The study identified a specific enzyme in baker's yeast, Gcy1, responsible for the conversion of d-GalUA to l-GalA, shedding light on the process of valorising d-GalUA in Saccharomyces cerevisiae.
Article
Engineering, Environmental
Hao Li, Yu Yang, Yumei Hu, Chun-Chi Chen, Jian-Wen Huang, Jian Min, Longhai Dai, Rey-Ting Guo
Summary: This study presents the three-dimensional structure and glyphosate-binding mode of two aldo-keto reductase enzymes in Echinochloa colona, as well as a possible mechanism of glyphosate degradation mediated by these enzymes. Additionally, a variant of one of the enzymes showed increased glyphosate degradation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Andrea J. Detlefsen, Ryan D. Paulukinas, Trevor M. Penning
Summary: Steroid hormone-transforming enzymes play a crucial role in regulating the functions of steroid hormones. Mutations in these enzymes can cause endocrine disorders and have significant impacts on sexual development and other severe disorders. This review focuses on disease-associated germline coding mutations in the aldo-keto reductase (AKR) superfamily, uncovering the effects of these mutations on androgen metabolism, bile acid deficiency, and other diseases. However, further research is needed to fully understand the relationship between AKR mutations and diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mubashir Aziz, Syeda Abida Ejaz, Nissren Tamam, Farhan Siddique
Summary: This study identifies theobromine and theophylline as strong inhibitors of AKR1B1 and AKR1B10, with potential anti-cancer activity against various tumor proteins. The findings contribute to the development and repurposing of AKR1B1/AKR1B10 inhibitors for the treatment of colon cancer and associated malignancies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Ronghua Chen, Siwei Wang, Yue Sun, Haiqing Li, Shuqing Wan, Fei Lin, Hanhong Xu
Summary: Genes that participate in the degradation or isolation of glyphosate in plants have the potential to confer herbicide tolerance and low glyphosate residue in crops. The aldo-keto reductase (AKR4) gene in Echinochloa colona (EcAKR4) has been identified as a naturally evolved glyphosate-metabolism enzyme. This study compared the glyphosate-degradation ability of AKR4 proteins from maize, soybean, and rice, revealing that ZmAKR4 had the highest activity and OsAKR4-1 and OsAKR4-2 exhibited the highest activity among the AKR4 family in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kohji Yamamoto, Satoshi Endo
Summary: We studied the effects of green leaf volatiles emitted by plants on insects, focusing on AKR2E9, a potential insect-resistance gene in silkworms. We found that AKR2E9 might regulate the detoxification of toxic aldehydes in the midgut and antennae of silkworms.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyu Guan, Lei Yu, Aoxue Wang
Summary: This study identified and analyzed 28 AKR family genes in tomatoes, revealing their potential roles in the resistance and tolerance of tomatoes to biotic and abiotic stresses. The expression patterns of these genes were also monitored in different tissues and in response to drought and salt stresses. Furthermore, the silencing of three candidate genes showed their sensitivity to drought and salt stresses, indicating their involvement in stress responses in tomatoes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Shu-Fang Li, Jian-Yong Xie, Shuai Qiu, Shen-Yuan Xu, Feng Cheng, Ya-Jun Wang, Yu-Guo Zheng
Summary: Enzyme engineering of an aldo-keto reductase (AKR) KmAKR for enhancing its thermostability and catalytic activity was successfully achieved through semirational design, resulting in improved performance for key chiral building blocks synthesis.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Carolina Di Benedetto, Carla Borini Etichetti, Nabila Cocordano, Alejo Cantoia, Evelyn Arel Zalazar, Silvio Bicciato, Mauricio Menacho-Marquez, German Leandro Rosano, Javier Girardini
Summary: Alteration of metabolism in cancer cells is crucial for sustaining aggressive traits. This study reveals that the expression of AKR1B1, controlled by the p53 tumor suppressor, is reduced in breast cancer patients. Furthermore, overexpression of AKR1B1 promotes metastasis and has a significant impact on proteins related to metabolism.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Burak Mugdat Karan, Karis Little, Josy Augustine, Alan W. Stitt, Tim M. Curtis
Summary: Diabetic retinopathy (DR) is a complication of diabetes mellitus that can cause vision loss and blindness. This review article explores the impact of oxidative stress, lipid-derived aldehydes, and advanced lipoxidation end products (ALEs) on the progression of DR. It also highlights the importance of aldehyde metabolism in the retina, particularly the role of aldehyde dehydrogenase (ALDH) and aldo-keto reductase (AKR) enzymes. The review discusses potential therapeutic strategies, such as gene therapy and pharmacological compounds, that can enhance the expression and activity of ALDH and AKR enzymes to protect the retina and preserve vision in diabetic patients.
Article
Biotechnology & Applied Microbiology
Ruixuan Bai, Baoling Chen, Liangyu Zheng
Summary: In this study, iolS from Bacillus subtilis 168 was identified as a potential biocatalyst for the asymmetric reduction of alpha-keto amides to yield chiral alpha-hydroxy amides. Through semi-rational engineering, stereocomplementary variants with high enantioselectivity were obtained, providing an effective strategy for obtaining alpha-hydroxy amides with high optical purity and offering insights into altering the stereoselectivity of AKRs.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Francesc Xavier Ruiz, Xavier Pares, Jaume Farres
Summary: Human aldo-keto reductase 1B10 (AKR1B10) is overexpressed in many cancer types and has been identified as an interesting drug target. Differences in inhibitor binding between AKR1B10 and aldose reductase (AR) have been observed, highlighting specific features that a selective AKR1B10 inhibitor should possess.
Article
Food Science & Technology
Azadeh Beizaei, Sara L. O' Kane, Abolfazl Kamkar, Alil Misaghi, Gary Henehan, Dolores J. Cahill
Article
Biochemical Research Methods
P. Kumar, B. Ryan, G. T. M. Henehan
PROTEIN EXPRESSION AND PURIFICATION
(2017)
Article
Biochemistry & Molecular Biology
Kumari Alka, Henry. J. Windle, Denise Cornally, Barry. J. Ryan, Gary T. M. Henehan
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Kumari Alka, J. Oliver Dolly, Barry J. Ryan, Gary T. M. Henehan
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Dharmendra S. Dheeman, Sanjay Antony-Babu, Jesus M. Frias, Gary T. M. Henehan
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2011)
Article
Audiology & Speech-Language Pathology
Aoife C. Kelly, Sara M. Boyd, Gary T. M. Henehan, Gordon Chambers
Review
Biotechnology & Applied Microbiology
Priyanka Priyanka, Yeqi Tan, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
BIOTECHNOLOGY LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Padraig Shanahan, Jeffrey O'Sullivan, Keith F. Tipton, Gemma K. Kinsella, Barry J. Ryan, Gary T. M. Henehan
JOURNAL OF FOOD BIOCHEMISTRY
(2019)
Article
Biochemical Research Methods
Priyanka Priyanka, Gemma Kinsella, Gary T. Henehan, Barry J. Ryan
PROTEIN EXPRESSION AND PURIFICATION
(2019)
Article
Biochemistry & Molecular Biology
Yeqi Tan, Gary T. Henehan, Gemma K. Kinsella, Barry J. Ryan
Summary: An extracellular lipase from Amycolatopsis mediteranei (AML) was cloned and studied for potential applications in process biotechnology, with its 3D structure elucidated by comparative modelling. AML was found to be a cutinase with specificity for medium chain fatty acyl moieties and capable of rapidly hydrolyzing certain aliphatic plastics under mild conditions. The findings suggest that minor changes in the active site region of AML can significantly affect substrate preference, expanding the potential applications for bioremediation of plastics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Wiem Haj Ahmed, Nathalie Boulet, Anais Briot, Barry J. Ryan, Gemma K. Kinsella, Jeffrey O'Sullivan, Francisco Les, Josep Mercader-Barcelo, Gary T. M. Henehan, Christian Carpene
Summary: Caffeine has direct inhibitory effects on glucose transport and lipogenesis in adipocytes. The findings suggest the potential of caffeine in the treatment or prevention of obesity complications.
Article
Biotechnology & Applied Microbiology
Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
Summary: This study identified a strain of Listeria monocytogenes that secretes solvent tolerant lipase activity. The organism showed resistance to solvents commonly used in biotechnological applications. However, practical applications would require the use of non-pathogenic or attenuated strains of Listeria.
BIOTECHNOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Albert Uhoraningoga, Gemma K. Kinsella, Jesus M. Frias, Gary T. Henehan, Barry J. Ryan
BIOENGINEERING-BASEL
(2019)
Article
Education & Educational Research
Aoife C. Kelly, Sara M. Boyd, Gary T. M. Henehan
HEALTH EDUCATION JOURNAL
(2015)