Article
Biochemistry & Molecular Biology
Gerd Gade, Heather G. Marco
Summary: The Indian stick insect, Carausius morosus, synthesizes two hypertrehalosemic hormones (HrTHs)-decapeptides in the corpora cardiaca (CC). One of the peptides, Carmo-HrTH-I, is modified by a C-mannosylated tryptophan residue at position 8. The synthetic form of Carmo-HrTH-I has been found to be stable, capable of inducing hyperlipemia and hypertrehalosemia, and resistant to enzymatic breakdown.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kento Mori, Hongkai Sun, Kazuki Miura, Siro Simizu
Summary: DPY19L3 is essential for myogenic differentiation, and its knockout affects cell differentiation, leading to suppression of MEK/ERK and p70S6K signaling pathways.
Article
Biochemistry & Molecular Biology
Hayato Mizuta, Ayane Takakusaki, Takehiro Suzuki, Keisuke Otake, Naoshi Dohmae, Siro Simizu
Summary: C-mannosylation is a unique type of protein glycosylation that affects protein functions, including secretion, intracellular localization, and stability. This study identifies a new C-mannosylated protein, RAMP1, and demonstrates that C-mannosylation enhances protein stability and cell migration activity.
Article
Biochemistry & Molecular Biology
Steven J. Berardinelli, Andrew R. Sillato, Richard C. Grady, Sanjiv Neupane, Atsuko Ito, Robert S. Haltiwanger, Bernadette C. Holdener
Summary: Thrombospondin 1 (THBS1) is a secreted extracellular matrix glycoprotein with diverse functions. Its modular domains interact with various proteins in the extracellular matrix to regulate cellular and physiological processes. The modifications of THBS1, such as O-linked glucose-fucose disaccharide and C-mannose, may affect its trafficking and function.
Review
Biochemistry & Molecular Biology
Samuel L. Crine, K. Ravi Acharya
Summary: C-mannosylation is a rare form of glycosylation that enhances the structural and functional diversity of proteins by forming a carbon-carbon bond with a tryptophan residue. Despite being discovered in 1994, it is still poorly understood and further research is needed to explore its mechanisms and functions.
Article
Biochemistry & Molecular Biology
Hirak Saxena, Nakita Buenbrazo, Won-Yong Song, Connie Li, Denis Brochu, Anna Robotham, Wen Ding, Luc Tessier, Rui Chen, John Kelly, Warren Wakarchuk
Summary: We have investigated the glycoproteome of the Actinobacterial species Cellulomonas fimi ATCC484 and identified 19 cell-associated glycoproteins using ConA lectin chromatography and mass spectrometry. Furthermore, we expressed selected C. fimi proteins in a mannosylation capable host and found that the glycosylation machinery in the host could glycosylate the recombinant proteins matching the native glycosylation in C. fimi. This observation sets the stage for further dissection of the biosynthetic machinery and biological consequences of protein mannosylation.
Article
Biochemistry & Molecular Biology
Satoshi Yoshimoto, Takehiro Suzuki, Naoki Otani, Daisuke Takahashi, Kazunobu Toshima, Naoshi Dohmae, Siro Simizu
Summary: C-mannosylation is a rare type of protein glycosylation that affects protein secretion, intracellular localization, and stability. In this study, the VMO1 protein was found to be C-mannosylated at Trp(105), leading to destabilization and conformational changes in the protein.
Article
Biochemistry & Molecular Biology
Joel S. Bloch, Alan John, Runyu Mao, Somnath Mukherjee, Jeremy Boilevin, Rossitza N. Irobalieva, Tamis Darbre, Nichollas E. Scott, Jean-Louis Reymond, Anthony A. Kossiakoff, Ethan D. Goddard-Borger, Kaspar P. Locher
Summary: C-linked glycosylation is essential for the trafficking, folding, and function of secretory and transmembrane proteins involved in cellular communication processes. Cryogenic-electron microscopy structures of Caenorhabditis elegans CMT reveal how the CMT recognizes the glycosylation sequon and triggers conformational activation of the donor substrate, enabling C-glycosylation of proteins. These findings have implications for understanding human diseases and potential therapeutic targeting of specific CMT paralogs.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Microbiology
Eun Jung Thak, Ye Ji Son, Dong-Jik Lee, Hyunah Kim, Jung Ho Kim, Su-Bin Lee, Yu-Byeong Jang, Yong-Sun Bahn, Connie B. Nichols, J. Andrew Alspaugh, Hyun Ah Kang
Summary: Cryptococcus neoformans assembles two types of O-linked glycans on its surface proteins, the more abundant major O-glycans that do not contain xylose residues and minor O-glycans containing xylose. The Cap6 alpha 1,3-mannosyltransferase plays a role in the synthesis of minor O-glycans, and its deletion affects various pathobiological processes in Cryptococcus neoformans.
Article
Biochemistry & Molecular Biology
Satoshi Yoshimoto, Kazuhiro Katayama, Takehiro Suzuki, Naoshi Dohmae, Siro Simizu
Summary: This study demonstrated that ISM1 is C-mannosylated at Trp(223) and Trp(226) in the TSR domain, and the C-mannosylation status affects its N-glycosylation. In addition, the secretion of ISM1 significantly decreased in cells expressing a C-mannosylation-defective mutant ISM1.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Ryota Kawahara, Tomoko Usami, Satoko Arakawa, Hiroki Kamo, Takehiro Suzuki, Ryosuke Komatsu, Hiroyuki Hara, Yuki Niwa, Erina Shimizu, Naoshi Dohmae, Shigeomi Shimizu, Siro Simizu
Summary: PMEL is a melanocyte-specific glycoprotein involved in melanosome maturation. It undergoes C-mannosylation at multiple tryptophan residues in its core amyloid fragment and N-terminal fragment. The C-mannosylation of PMEL is required for proper melanosome development and fibril formation.
Article
Multidisciplinary Sciences
Yu-Pu Jing, Xinpeng Wen, Lunjie Li, Shanjing Zhang, Ci Zhang, Shutang Zhou
Summary: Using the migratory locust as a model system, the study found that juvenile hormone promotes phosphorylation of Vitellogenin receptor, which is essential for Vitellogenin uptake and recycling. This phosphorylation regulation is evolutionarily conserved in other insects as well.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Xinyuan Cui, Xiaosong Pei, Hao Wang, Ping Feng, Huamin Qin, Shuai Liu, Qiu Yan, Jiwei Liu
Summary: Peritoneal metastasis is a major factor contributing to poor prognosis and high mortality in ovarian cancer patients. This study demonstrates that elevated levels of ALG3 and alpha 1,3-mannosylation are associated with ovarian cancer malignancy and poor patient prognosis. The downregulation of ALG3 inhibits proliferation and peritoneal metastasis of ovarian cancer cells. Additionally, ALG3 enhances uPAR activation and promotes ovarian cancer peritoneal metastasis through the ADAM8/Ras/ERK pathway. Overall, ALG3 could serve as a potential diagnostic biomarker and therapeutic target for ovarian cancer.
Article
Biochemistry & Molecular Biology
Lucile Senicourt, Albane le Maire, Frederic Allemand, JoAo E. Carvalho, Laura Guee, Pierre Germain, Michael Schubert, Pau Bernado, William Bourguet, Nathalie Sibille
Summary: Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) form heterodimers to activate target gene transcription by recruiting co-activator complexes. The nuclear receptor co-activator TIF2 interacts with the ligand-binding domain of NRs to mediate recruitment, showing a largely disordered protein with partially structured regions, including the NR-boxes and their evolutionary conserved flanking regions. NMR and X-ray crystallographic data reveal a multisite binding of the NR-boxes and an active role of their flanking regions in the interaction.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Boning Cao, Zhongqiang Xia, Zhanzhang Hao, Changying Liu, Dingpei Long, Wei Fan, Aichun Zhao
Summary: The study investigated the function of MnNHX6, an endosomal-type NHX in mulberry, and found that the conserved region of its C-terminus plays a crucial role in protein function, stability, and interaction with other proteins. These findings contribute to a better understanding of the role of the C-terminal tail of NHXs in plants and the ion transport mechanism of NHX-like antiporters.
Article
Chemistry, Multidisciplinary
Roland Frach, Patrick Kibies, Saraphina Boettcher, Tim Pongratz, Steven Strohfeldt, Simon Kurrmann, Joerg Koehler, Martin Hofmann, Werner Kremer, Hans Robert Kalbitzer, Oliver Reiser, Dominik Horinek, Stefan M. Kast
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Biochemistry & Molecular Biology
Paulo H. C. Godoi, Rachel P. Wilkie-Grantham, Asami Hishiki, Renata Sano, Yasuko Matsuzawa, Hiroko Yanagi, Claudia E. Munte, Ya Chen, Yong Yao, Francesca M. Marassi, Hans R. Kalbitzer, Shu-ichi Matsuzawa, John C. Reed
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Michael Spoerner, Matthias Karl, Pedro Lopes, Marcus Hoering, Karoline Loeffel, Andrea Nuehs, Joseph Adelsberger, Werner Kremer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Biochemistry & Molecular Biology
Markus Beck Erlach, Joerg Koehler, Edson Crusca, Werner Kremer, Claudia E. Munte, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2016)
Article
Multidisciplinary Sciences
Sunilkumar Puthenpurackal Narayanan, Divya Gopalakrishnan Nair, Daniel Schaal, Marisa Barbosa de Aguiar, Sabine Wenzel, Werner Kremer, Stephan Schwarzinger, Hans Robert Kalbitzer
SCIENTIFIC REPORTS
(2016)
Article
Chemistry, Multidisciplinary
Markus Beck Erlach, Hans Robert Kalbitzer, Roland Winter, Werner Kremer
Article
Biochemistry & Molecular Biology
Tobias Harsch, Philipp Schneider, Barbel Kieninger, Harald Donaubauer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Biochemistry & Molecular Biology
Markus Beck Erlach, Joerg Koehler, Edson Crusca, Claudia E. Munte, Masatsune Kainosho, Werner Kremer, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Chemistry, Multidisciplinary
Italo A. Cavini, Claudia E. Munte, Markus Beck Erlach, Thomas van Groen, Inga Kadish, Tao Zhang, Tamar Ziehm, Luitgard Nagel-Steger, Janine Kutzsche, Werner Kremer, Dieter Willbold, Hans Robert Kalbitzer
CHEMICAL COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Daniela Baumstark, Werner Kremer, Alfred Boettcher, Christina Schreier, Paul Sander, Gerd Schmitz, Renate Kirchhoefer, Fritz Huber, Hans Robert Kalbitzer
JOURNAL OF LIPID RESEARCH
(2019)
Article
Biophysics
Raphael Kreitner, Claudia E. Munte, Katrin Singer, Karl O. Stetter, Gudrun Horn, Werner Kremer, Hans Robert Kalbitzer
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biochemistry & Molecular Biology
Markus Beck Erlach, Joerg Koehler, Claudia E. Munte, Werner Kremer, Edson Crusca, Masatsune Kainosho, Hans Robert Kalbitzer
JOURNAL OF BIOMOLECULAR NMR
(2020)
Article
Chemistry, Multidisciplinary
Eric Girard, Pedro Lopes, Michael Spoerner, Anne-Claire Dhaussy, Thierry Prange, Hans Robert Kalbitzer, Nathalie Colloc'h
Summary: In this study, the researchers used high pressure crystallography to investigate the allosteric transitions of Ras protein. They found that pressure can shift the equilibria between different conformational states, providing insight into the structural plasticity involved in Ras protein's allosteric equilibria.
Article
Biochemistry & Molecular Biology
Konstanze von Koenig, Norman Kachel, Hans Robert Kalbitzer, Werner Kremer
Article
Biochemistry & Molecular Biology
Matthias Karl, Michael Spoerner, Thuy-Vy Pham, Sunilkumar Puthenpurackal Narayanan, Werner Kremer, Hans Robert Kalbitzer
BIOPHYSICAL CHEMISTRY
(2017)