Article
Multidisciplinary Sciences
Wan-Chen Li, Chia-Yi Lee, Wei-Hsuan Lan, Tai-Ting Woo, Hou-Cheng Liu, Hsin-Yi Yeh, Hao-Yen Chang, Yu-Chien Chuang, Chiung-Ya Chen, Chi-Ning Chuang, Chia-Ling Chen, Yi-Ping Hsueh, Hung-Wen Li, Peter Chi, Ting-Fang Wang
Summary: Most eukaryotes possess two RecA-like recombinases for interhomolog recombination during meiosis, but some have lost Dmc1. TrRad51 in Trichoderma reesei plays a crucial role in interhomolog recombination and has better mismatch tolerance than ScRad51, evolving ScDmc1-like properties through structural variations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Hideo Tsubouchi
Summary: Homologous recombination (HR) is crucial for meiosis and is induced during meiotic prophase. The Hop2-Mnd1 complex, originally identified in budding yeast, is conserved across species and plays an essential role in meiosis. Accumulating evidence suggests that Hop2-Mnd1 promotes RecA-like recombinases for homology search and strand exchange. This review summarizes studies on the mechanism of the Hop2-Mnd1 complex in promoting HR and beyond.
Article
Biochemistry & Molecular Biology
Kei Yamaya, Bin Wang, Nadin Memar, Arome Solomon Odiba, Alexander Woglar, Anton Gartner, Anne M. Villeneuve
Summary: RAD54 family DNA translocases, together with RAD51 recombinases, play a role in maintaining genome stability. Studying the functions of RAD54 paralogs RAD-54.L and RAD-54.B in Caenorhabditis elegans during meiotic prophase reveals their distinct contributions to the dynamics of RAD-51 association with DNA and meiotic double-strand break repair. RAD-54.L is essential for RAD-51 removal from meiotic double-strand break sites, while RAD-54.B prevents excessive accumulation of RAD-51 on unbroken DNA. This study provides insights into the division of labor among RAD-54 paralogs in promoting efficient homologous recombination and preventing deleterious effects of unproductive RAD-51 association.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Plant Sciences
Jason Sims, Fernando A. Rabanal, Christiane Elgert, Arndt Von Haeseler, Peter Schloegelhofer
Summary: Ribosomal RNA genes are organized in large domains of hundreds of rDNA units, with stable inheritance crucial for survival. During meiosis, rDNA loci are embedded within the nucleolus favoring non-homologous end joining as a repair mechanism, while rare homologous recombination events dynamically change rDNA unit numbers. The sequence heterogeneity and size stability of rDNA clusters in Arabidopsis thaliana show potential functional implications and drive further research into the mechanisms maintaining their metastable state.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shuying Yang, Chao Zhang, Yiwei Cao, Guijie Du, Ding Tang, Yafei Li, Yi Shen, Hengxiu Yu, Zhukuan Cheng
Summary: FIGNL1 functions as an inhibitor for non-homologous chromosome interaction and crossover formation during rice meiosis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xiaofei Liu, Yiwei Cao, Guijie Du, Chao Zhang, Meng Xu, Zhukuan Cheng, Yi Shen, Hengxiu Yu
Summary: The mechanisms of OsRAD51 and OsDMC1 in rice meiosis are still unknown. In this study, mutant plants for Osrad51a1, Osrad51a2, Osdmc1a, and Osdmc1b were obtained through CRISPR/Cas9, and it was found that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility. The study also suggests that OsRAD51 and OsDMC1 may functionally depend on each other and play important roles in meiotic recombination during meiosis in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Haibo Xie, Xiaosi Wang, Minjun Jin, Lanqin Li, Junwen Zhu, Yunsi Kang, Zhe Chen, Yonghua Sun, Chengtian Zhao
Summary: Meiosis is crucial for evolution and genetic diversity, and cilia play a critical role in the meiotic recombination process. Our study reveals the involvement of cilia in meiosis and suggests that extracellular signals may regulate meiotic recombination through this organelle.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yu Jiang, Fei Huang, Lu Chen, Jia-Hui Gu, Yun-Wen Wu, Meng-Yan Jia, Zhen Lin, Yong Zhou, Yan-Chu Li, Chao Yu, Ming-Han Tong, Li Shen, Heng-Yu Fan, Qian-Qian Sha
Summary: The R-loop, a three-strand nucleic acid structure, plays important regulatory roles in the meiosis process, particularly in transcriptional regulation and DNA repair. The study of R-loop in mice revealed its predominant distribution at promoter-related regions, its association with high transcription activity, and its crucial role in fertility and DSB repair. These findings highlight the significance of R-loop in spermatogenesis.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Marina Martinez-Garcia, Charles I. White, F. Chris. H. Franklin, Eugenio Sanchez-Moran
Summary: TOPII plays an essential endogenous role in normal root growth, cell cycle regulation, mitotic DNA repair through homologous recombination, and meiotic DNA repair progression in Arabidopsis thaliana. While it is required for meiotic double-strand break repair, it does not affect crossover formation. It is proposed that TOPII may facilitate mitotic homologous recombination DNA repair by relieving stress necessary for strand invasion and D-loop formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Come Emmenecker, Christine Mezard, Rajeev Kumar
Summary: Homologous recombination plays a crucial role in meiosis for DNA repair, balanced chromosome segregation, and genetic variation. RAD51 and DMC1 are recombinases that form nucleoprotein filaments and are important for meiotic DNA repair and genome stability. This review summarizes the current understanding of their functions and regulation, as well as the models and regulators of homology search and strand exchange conserved in plant meiosis. Manipulation of these repair factors in plant meiosis has the potential to accelerate crop breeding and improve productivity.
PLANT REPRODUCTION
(2022)
Article
Cell Biology
Kaixian Liu, Emily M. Grasso, Stephen Pu, Mengyang Zou, Shixin Liu, David Eliezer, Scott Keeney
Summary: This study reports the structure and dynamic DNA binding properties of the Rec114 and Mei4 protein complex, which initiates DNA double-strand breaks during meiotic recombination. The complex can bridge multiple DNA duplexes and generate force to condense DNA through long-range interactions. These findings provide insight into the conserved networks of protein-protein and protein-DNA interactions that promote condensate formation and meiotic DSBs.
GENES & DEVELOPMENT
(2023)
Article
Plant Sciences
Ayako Nishizawa-Yokoi, Ritsuko Motoyama, Tsuyoshi Tanaka, Akiko Mori, Keiko Iida, Seiichi Toki
Summary: Land plants have developed defense systems, including DNA damage response (DDR) and DNA repair systems, to protect themselves. In rice, the transcription factor OsSOG1 plays a crucial role in controlling DDR and DNA repair and has differences compared to Arabidopsis.
Review
Cell Biology
Chengzi Huang, Ting Guo, Yingying Qin
Summary: POI is the early depletion of ovarian function before 40 years of age due to various causes, including genetic factors and unknown reasons. Advances in next-generation sequencing have expanded the genetic spectrum of POI, particularly in identifying genes related to meiosis and DNA repair. Understanding the genetic architecture of POI can help predict risks, protect ovarian function, and intervene early for affected women.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Xiang Li, Mingsen Yu, Pablo Bolanos-Villegas, Jun Zhang, Di'an Ni, Hong Ma, Yingxiang Wang
Summary: The study revealed the crucial role of FANCM in shaping the distribution of meiotic Class-I crossovers in plants, with evolutionary divergent roles observed between Arabidopsis and lettuce in meiotic bivalent formation.
Article
Plant Sciences
Pablo Parra-Nunez, Nadia Fernandez-Jimenez, Miguel Pachon-Penalba, Eugenio Sanchez-Moran, Monica Pradillo, Juan Luis Santos
Summary: Mutations affecting crossover frequency and distribution during meiosis can result in aneuploid gametes and sterility. The cytogenetic consequences of colchicine-induced autotetraploids from Arabidopsis mutants with altered crossover frequency were analyzed, revealing the potential of these mutants for studying key proteins in plant meiosis.
Review
Biochemistry & Molecular Biology
Masako Koyama, Hitoshi Kurumizaka
JOURNAL OF BIOCHEMISTRY
(2018)
Article
Andrology
K. Shiraishi, A. Shindo, A. Harada, H. Kurumizaka, H. Kimura, Y. Ohkawa, H. Matsuyama
Article
Cell Biology
Atsuhiko Fukuto, Masae Ikura, Tsuyoshi Ikura, Jiying Sun, Yasunori Horikoshi, Hiroki Shima, Kazuhiko Igarashi, Masayuki Kusakabe, Masahiko Harata, Naoki Horikoshi, Hitoshi Kurumizaka, Yoshiaki Kiuchi, Satoshi Tashiro
Article
Biochemistry & Molecular Biology
Akihisa Osakabe, Zdravko J. Lorkovic, Wataru Kobayashi, Hiroaki Tachiwana, Ramesh Yelagandula, Hitoshi Kurumizaka, Frederic Berger
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Akihito Harada, Kazumitsu Maehara, Yusuke Ono, Hiroyuki Taguchi, Kiyoshi Yoshioka, Yasuo Kitajima, Yan Xie, Yuko Sato, Takeshi Iwasaki, Jumpei Nogami, Seiji Okada, Tetsuro Komatsu, Yuichiro Semba, Tatsuya Takemoto, Hiroshi Kimura, Hitoshi Kurumizaka, Yasuyuki Ohkawa
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Yoshimasa Takizawa, Hiroki Tanaka, Shinichi Machida, Masako Koyama, Kazumitsu Maehara, Yasuyuki Ohkawa, Paul A. Wade, Matthias Wolf, Hitoshi Kurumizaka
Article
Biochemical Research Methods
Yasuhiro Arimura, Tomonori Kono, Kuniki Kino, Hitoshi Kurumizaka
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Kana Tanabe, Jiaan Liu, Daiki Kato, Hitoshi Kurumizaka, Kenzo Yamatsugu, Motomu Kanai, Shigehiro A. Kawashima
SCIENTIFIC REPORTS
(2018)
Correction
Biochemistry & Molecular Biology
Wakana Iwasaki, Yuta Miya, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroaki Tachiwana, Takehiko Shibata, Wataru Kagawa, Hitoshi Kurumizaka
Article
Cell Biology
Akihito Harada, Kazumitsu Maehara, Tetsuya Handa, Yasuhiro Arimura, Jumpei Nogami, Yoko Hayashi-Takanaka, Katsuhiko Shirahige, Hitoshi Kurumizaka, Hiroshi Kimura, Yasuyuki Ohkawa
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Tomoya Kujirai, Haruhiko Ehara, Yuka Fujino, Mikako Shirouzu, Shun-ichi Sekine, Hitoshi Kurumizaka
Article
Multidisciplinary Sciences
Lot Snijders Blok, Justine Rousseau, Joanna Twist, Sophie Ehresmann, Motoki Takaku, Hanka Venselaar, Lance H. Rodan, Catherine B. Nowak, Jessica Douglas, Kathryn J. Swoboda, Marcie A. Steeves, Inderneel Sahai, Connie T. R. M. Stumpel, Alexander P. A. Stegmann, Patricia Wheeler, Marcia Willing, Elise Fiala, Aaina Kochhar, William T. Gibson, Ana S. A. Cohen, Ruky Agbahovbe, A. Micheil Innes, P. Y. Billie Au, Julia Rankin, Ilse J. Anderson, Steven A. Skinner, Raymond J. Louie, Hannah E. Warren, Alexandra Afenjar, Boris Keren, Caroline Nava, Julien Buratti, Arnaud Isapof, Diana Rodriguez, Raymond Lewandowski, Jennifer Propst, Ton van Essen, Murim Choi, Sangmoon Lee, Jong H. Chae, Susan Price, Rhonda E. Schnur, Ganka Douglas, Ingrid M. Wentzensen, Christiane Zweier, Andre Reis, Martin G. Bialer, Christine Moore, Marije Koopmans, Eva H. Brilstra, Glen R. Monroe, Koen L. van Gassen, Ellen van Binsbergen, Ruth Newbury-Ecob, Lucy Bownass, Ingrid Bader, Johannes A. Mayr, Saskia B. Wortmann, Kathy J. Jakielski, Edythe A. Strand, Katja Kloth, Tatjana Bierhals, John D. Roberts, Robert M. Petrovich, Shinichi Machida, Hitoshi Kurumizaka, Stefan Lelieveld, Rolph Pfundt, Sandra Jansen, Pelagia Deriziotis, Laurence Faivre, Julien Thevenon, Mirna Assoum, Lawrence Shriberg, Tjitske Kleefstra, Han G. Brunner, Paul A. Wade, Simon E. Fisher, Philippe M. Campeau
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Tomoya Kujirai, Christian Zierhut, Yoshimasa Takizawa, Ryan Kim, Lumi Negishi, Nobuki Uruma, Seiya Hirai, Hironori Funabiki, Hitoshi Kurumizaka
Review
Cell Biology
Tomoya Kujirai, Haruhiko Ehara, Shun-ichi Sekine, Hitoshi Kurumizaka
Summary: In eukaryotes, genomic DNA is packaged into chromatin and the nucleosome acts as a barrier to transcription. The RNA polymerase II elongation complex disassembles the nucleosome during transcription to allow passage. After transcription, the nucleosome is rebuilt by transcription-coupled nucleosome reassembly, which plays a crucial role in preserving epigenetic information. The histone chaperone FACT is involved in nucleosome disassembly, maintenance, and reassembly during transcription in chromatin. Recent structural studies have provided insights into the structural transitions of the nucleosome during transcription.
Article
Multidisciplinary Sciences
Masahiro Nishimura, Yoshimasa Takizawa, Kayo Nozawa, Hitoshi Kurumizaka
Summary: The study reveals the structure of p53 binding to a nucleosome, providing valuable insights into the mechanism by which p53 binds to its target DNA and changes the chromatin structure for gene activation.