Article
Chemistry, Multidisciplinary
Kathleen A. Trychta, Bing Xie, Ravi Kumar Verma, Min Xu, Lei Shi, Brandon K. Harvey
Summary: Recent research shows that the carboxy tail sequences of ER resident proteins can stably bind to Golgi KDELRs, not limited to the classic KDEL sequence, aiding in predicting whether ER resident proteins will undergo secretion in response to ER calcium depletion.
FRONTIERS IN CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Emily S. Wires, Kathleen A. Trychta, Lacey M. Kennedy, Brandon K. Harvey
Summary: The KDEL receptor retrieval pathway is crucial for maintaining ER resident proteins, but can be disrupted by perturbations like calcium depletion and UPR activation. Recent data shows that KDEL receptors are activated through the IRE1/XBP1 signaling pathway during UPR as an adaptive response to cellular stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Xiangui Meng, Weiquan Li, Hongwei Yuan, Wei Dong, Wen Xiao, Xiaoping Zhang
Summary: In this study, we identified KDELR2 as a potential risk factor with prognostic value in bladder cancer patients. We also found that KDELR2 is a regulator of BCa cell proliferation and tumorigenicity, and its mechanism involves the regulation of KIF20A expression.
BIOLOGICAL PROCEDURES ONLINE
(2022)
Article
Multidisciplinary Sciences
Jonas C. Alvim, Robert M. Bolt, Jing An, Yasuko Kamisugi, Andrew Cuming, Fernanda A. L. Silva-Alvim, Juan O. Concha, Luis L. P. da Silva, Meiyi Hu, Dominique Hirsz, Jurgen Denecke
Summary: In contrast to the current model based on fast recycling of the K/HDEL receptor (ERD2) between the ER and the Golgi apparatus, the authors show evidence that ERD2 is specifically retained in the Golgi apparatus and evades joining its ligands en route to the ER.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Mareile Boschanski, Tobias Krueger, Lennard Karsten, Georg Falck, Sarfaraz Alam, Marcus Gerlach, Benjamin Mueller, Kristian M. Mueller, Norbert Sewald, Thomas Dierks
Summary: This study demonstrates an improved and facile strategy for functionalizing DARPin and scFv425-Fc antibodies targeting EGFR, by conjugating them with MMAE and CF for successful testing in cell culture for receptor binding, internalization, and cytotoxicity.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Plant Sciences
Xifeng Li, Zhe Wang, Yunting Fu, Xi Cheng, Yan Zhang, Baofang Fan, Cheng Zhu, Zhixiang Chen
Summary: UBAC2 is a multifunctional protein in plant cells that regulates the degradation and accumulation of specific proteins, including COPT and POWDERY MILDEW RESISTANCE 4.
Article
Multidisciplinary Sciences
Navit Ogen-Shtern, Chieh Chang, Haddas Saad, Niv Mazkereth, Chaitanya Patel, Marina Shenkman, Gerardo Z. Lederkremer
Summary: Misfolded proteins and components of the ER quality control and ERAD machineries accumulate in the pericentriolar ERQC, which serves as a staging ground for ERAD. Trafficking to the ERQC is reversible, with recycling back to the ER slower than movement in the ER periphery. Vesicular trafficking, rather than diffusion, is involved in this process. COPI inhibition leads to accumulation in the ERQC and increases ERAD, while COPII inhibition has the opposite effect. Targeting of misfolded proteins to ERAD involves COPII-dependent transport to the ERQC and retrieval to the peripheral ER in a COPI-dependent manner.
Article
Multidisciplinary Sciences
Ning Wang, Yoko Shibata, Joao A. Paulo, Steven P. Gygi, Tom A. Rapoport
Summary: Rop1 is the only member of a subfamily of membrane-curvature generating REEPs in fission yeast. Wang et al. demonstrate that Rop1 is crucial for macroautophagy by facilitating autophagosome formation. Rop1 functions in the generation of high membrane curvature and colocalizes with the autophagy component Atg2 at the phagophore rim.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Sanaz Dastghaib, P. Sravan Kumar, Sajjad Aftabi, Gautam Damera, Azadeh Dalvand, Adel Sepanjnia, Mohammad Kiumarsi, Mohamad-Reza Aghanoori, Sukhwinder Singh Sohal, Sudharsana R. Ande, Javad Alizadeh, Pooneh Mokarram, Saeid Ghavami, Pawan Sharma, Amir A. Zeki
Summary: Lung cells are exposed to internal and external stressors, leading to disruption of protein homeostasis and activation of the UPR. Dysregulation of the UPR is associated with disease development and various human conditions. Compounds targeting the UPR pathway show potential for future therapeutic interventions.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qiaoxia Zheng, Yong Chen, Di Chen, Hongyu Zhao, Yun Feng, Quan Meng, Yan Zhao, Hong Zhang
Summary: This study revealed the crucial role of Ca2+ transients in inducing phase separation of FIP200 on the ER and specifying autophagosome initiation sites.
Article
Cell Biology
Rui Yan, Kun Chen, Bowen Wang, Ke Xu
Summary: A highly elongated tubular ER-Golgi intermediate compartment (t-ERGIC) that selectively expedites the ER-to-Golgi transport for soluble cargoes of the receptor SURF4 has been discovered. The t-ERGIC has a distinctive elongated and thinned shape, and is marked by the small GTPases Rabl A/B instead of the canonical ERGIC marker ERGIC-53. It accelerates the trafficking of SURF4-bound cargoes with its large surface-to-volume ratio, high intracellular traveling speeds, and ER-Golgi recycling capabilities.
DEVELOPMENTAL CELL
(2022)
Article
Biotechnology & Applied Microbiology
Tianfang Shi, Jungang Zhou, Aijuan Xue, Hong Lu, Yungang He, Yao Yu
Summary: This study characterized the ER stress response in K. marxianus for the first time and identified ESRTs that can be manipulated to improve the production of heterologous proteins. The overexpression of genes related to carbohydrate metabolism and ERAD can enhance the secretion of heterologous proteins, while the deletion of certain genes can lead to significant improvements in protein secretion. This research provides insights into optimizing protein production by understanding and modulating the ER stress response in K. marxianus.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Cell Biology
F. C. Palazzo, R. Sitia, T. Tempio
Summary: In multicellular organisms, cells communicate through cognate interactions between membrane and secretory proteins. The folding of these proteins is assisted by chaperones and enzymes in the early secretory compartment (ESC), preventing their transport beyond the Golgi. Cells can selectively secrete certain ESC residents through differential intracellular distribution and release mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Timothy J. Hawkins, Michaela Kopischke, Patrick J. Duckney, Katarzyna Rybak, David A. Mentlak, Johan T. M. Kroon, Mai Thu Bui, A. Christine Richardson, Mary Casey, Agnieszka Alexander, Geert De Jaeger, Monika Kalde, Ian Moore, Yasin Dagdas, Patrick J. Hussey, Silke Robatzek
Summary: This study reveals that NET4A and NET4B members of the NET family play an essential role in the reorganization of guard cell actin during stomatal closure, mediating the molecular link between actin filaments and the tonoplast through interaction with RABG3b.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Diego Tapia, Viviana A. Cavieres, Patricia V. Burgos, Jorge Cancino
Summary: The conventional secretory pathway and autophagy are two interconnected cellular processes that are essential for maintaining cellular homeostasis. The secretory pathway synthesizes and delivers proteins to different locations, while autophagy engulfs damaged organelles and cytosolic constituents. These processes are mutually regulated, but the mechanisms are not well understood.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biophysics
Alexander Harder, Ann-Kristin Moeller, Fabian Milz, Phillipp Neuhaus, Volker Walhorn, Thomas Dierks, Dario Anselmetti
BIOPHYSICAL JOURNAL
(2015)
Editorial Material
Biochemistry & Molecular Biology
Jianhe Peng, Sarfaraz Alam, Karthikeyan Radhakrishnan, Malaiyalam Mariappan, Markus Georg Rudolph, Caroline May, Thomas Dierks, Kurt von Figura, Bernhard Schmidt
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Oncology
Susmita Mondal, Debarshi Roy, Juliana Camacho-Pereira, Ashwani Khurana, Eduardo Chini, Lifeng Yang, Joelle Baddour, Katherine Stilles, Seth Padmabandu, Sam Leung, Steve Kalloger, Blake Gilks, Val Lowe, Thomas Dierks, Edward Hammond, Keith Dredge, Deepak Nagrath, Viji Shridhar
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Cell Biology
Heike Wolf, Markus Damme, Stijn Stroobants, Rudi D'Hooge, Hans Christian Beck, Irm Hermans-Borgmeyer, Renate Luellmann-Rauch, Thomas Dierks, Torben Luebke
DISEASE MODELS & MECHANISMS
(2016)
Article
Endocrinology & Metabolism
Ilona Jaszczuk, Lars Schlotawa, Thomas Dierks, Andreas Ohlenbusch, Dominique Koppenhoefer, Mariusz Babicz, Monika Lejman, Karthikeyan Radhakrishnan, Agnieszka Lugowska
MOLECULAR GENETICS AND METABOLISM
(2017)
Article
Biochemistry & Molecular Biology
Bjoern Kowalewski, Peter Heimann, Theresa Ortkras, Renate Luellmann-Rauch, Tomo Sawada, Steven U. Walkley, Thomas Dierks, Markus Damme
HUMAN MOLECULAR GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Elena Marie Wiegmann, Eva Westendorf, Ina Kalus, Thomas H. Pringle, Torben Luebke, Thomas Dierks
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Eva C. Ennemann, Karthikeyan Radhakrishnan, Malaiyalam Mariappan, Michaela Wachs, Thomas H. Pringle, Bernhard Schmidt, Thomas Dierks
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
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Biochemistry & Molecular Biology
Bjoern Kowalewski, Torben Luebke, Katrin Kollmann, Thomas Braulke, Thomas Reinheckel, Thomas Dierks, Markus Damme
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Juliane Poppe, Eberhard Warkentin, Ulrike Demmer, Bjoern Kowalewski, Thomas Dierks, Klaus Schneider, Ulrich Ermler
JOURNAL OF INORGANIC BIOCHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Myungsik Yoo, Muntasir Khaled, Kurt M. Gibbs, Jonghun Kim, Bjoern Kowalewski, Thomas Dierks, Melitta Schachner
Article
Multidisciplinary Sciences
Ina Kalus, Susanne Rohn, Tania M. Puvirajesinghe, Scott E. Guimond, Pieter J. Eyckerman-Koelln, Gerdy ten Dam, Toin H. van Kuppevelt, Jeremy E. Turnbull, Thomas Dierks
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Multidisciplinary Sciences
Debarshi Roy, Susmita Mondal, Ashwani Khurana, Deok-Beom Jung, Robert Hoffmann, Xiaoping He, Eleftheria Kalogera, Thomas Dierks, Edward Hammond, Keith Dredge, Viji Shridhar
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Omkar P. Dhamale, Roger Lawrence, Elena M. Wiegmann, Bhahwal A. Shah, Kanar Al-Mafraji, William C. Lamanna, Torben Luebke, Thomas Dierks, Geert-Jan Boons, Jeffrey D. Esko
ACS CHEMICAL BIOLOGY
(2017)
Article
Ophthalmology
Katharina Kruszewski, Renate Luellmann-Rauch, Thomas Dierks, Udo Bartsch, Markus Damme
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)