Article
Multidisciplinary Sciences
Yicong Wu, Xiaofei Han, Yijun Su, Melissa Glidewell, Jonathan S. Daniels, Jiamin Liu, Titas Sengupta, Ivan Rey-Suarez, Robert Fischer, Akshay Patel, Christian Combs, Junhui Sun, Xufeng Wu, Ryan Christensen, Corey Smith, Lingyu Bao, Yilun Sun, Leighton H. Duncan, Jiji Chen, Yves Pommier, Yun-Bo Shi, Elizabeth Murphy, Sougata Roy, Arpita Upadhyaya, Daniel Colon-Ramos, Patrick La Riviere, Hari Shroff
Summary: Researchers have enhanced the performance of confocal microscopy using four approaches: developing compact line scanners, combining multi-view imaging, adapting techniques from structured illumination microscopy, and utilizing deep learning. They successfully improved imaging resolution, reduced phototoxicity, and demonstrated these advancements on over 20 different samples.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Optics
Mingwei Tang, Yubing Han, Danchen Jia, Qing Yang, Ji-Xin Cheng
Summary: Far-field chemical microscopy, with molecular electronic or vibrational fingerprint information, offers a new perspective for studying three-dimensional biological, material, and chemical systems. Despite the limitation of optics, recent advances in super-resolution techniques have overcome this hurdle, pushing the boundaries of far-field chemical microscopy in terms of spatial resolution. This technique finds applications in various fields including biomedical research, material characterization, environmental study, cultural heritage conservation, and integrated chip inspection.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biochemical Research Methods
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk, Cara R. Schiavon, Seungyoon B. Yu, Tong Zhang, Melissa Wu, Kyle Kastner, Alaa Abdel Latif, Zijun Lin, Andrew Shaw, Yoshiyuki Kubota, John Mendenhall, Zhao Zhang, Gulcin Pekkurnaz, Kristen Harris, Jeremy Howard, Uri Manor
Summary: Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
News Item
Multidisciplinary Sciences
Ewen Callaway
Summary: The technique surpasses the resolving power of multi-million-dollar machines.
Article
Biochemistry & Molecular Biology
Ivona Kubalova, Alzbeta Nemeckova, Klaus Weisshart, Eva Hribova, Veit Schubert
Summary: Fluorescence light microscopy is important for studying cellular structures, but limited by light diffraction; super-resolution techniques like SIM are used to overcome this limitation; however, STED and SMLM, which offer higher resolution, are not widely applied in plant cell research due to specific sample preparation requirements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Susanne Dechantsreiter, Ashley R. Ambrose, Jonathan D. Worboys, Joey M. E. Lim, Sylvia Liu, Rajesh Shah, M. Angeles Montero, Anne Marie Quinn, Tracy Hussell, Gillian M. Tannahill, Daniel M. Davis
Summary: Using super-resolution microscopy, this study compared extracellular vesicles (EVs) secreted by different human monocyte-derived macrophages (MDMs). The researchers found variations in EV size and content between different MDM phenotypes. The study also identified differential expression of a specific protein, HLA-DR, on EVs secreted by different MDM phenotypes, including lung macrophages isolated from cancer patients. This quantitative analysis of EV heterogeneity can provide insights into macrophage biology and has implications for single-cell profiling.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biotechnology & Applied Microbiology
H. J. Geertsema, G. Aimola, V Fabricius, J. P. Fuerste, B. B. Kaufer, H. Ewers
Summary: Left-handed DNA probes improve super-resolution DNA-PAINT imaging in the nucleus by reducing background signal, maintaining the same specificity and multiplexing capabilities as right-handed DNA probes.
NATURE BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Weisong Zhao, Shiqun Zhao, Liuju Li, Xiaoshuai Huang, Shijia Xing, Yulin Zhang, Guohua Qiu, Zhenqian Han, Yingxu Shang, De-En Sun, Chunyan Shan, Runlong Wu, Lusheng Gu, Shuwen Zhang, Riwang Chen, Jian Xiao, Yanquan Mo, Jianyong Wang, Wei Ji, Xing Chen, Baoquan Ding, Yanmei Liu, Heng Mao, Bao-Liang Song, Jiubin Tan, Jian Liu, Haoyu Li, Liangyi Chen
Summary: Sparse structured illumination microscopy (Sparse-SIM) achieves nearly twofold resolution enhancement by utilizing sparse deconvolution algorithm, enabling the resolution of intricate biological structures such as small fusion pores, nuclear pores, and relative movements of inner and outer mitochondrial membranes.
NATURE BIOTECHNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Miranda Parisi, Massimiliano Lucidi, Paolo Visca, Gabriella Cincotti
Summary: This article reviews various super-resolution microscopy techniques utilized by microbiologists to investigate bacterial cell morphology and functions. The achieved resolution is discussed, with a focus on fluorophore selection and other critical imaging requirements. Recent applications are described, which have provided novel insights into bacterial cellular structures. The pivotal role of super-resolution microscopy in addressing antimicrobial resistance is emphasized.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Review
Immunology
Yuan-hui Li, Yu Zhang, Gang Pan, Li-xin Xiang, Ding-cun Luo, Jian-zhong Shao
Summary: This review discusses the basic biology of Ly6C(hi) and Ly6C(lo) macrophages, including their origin, differentiation, and phenotypic switching, and their diverse functions in homeostasis and disease. Understanding these cells may provide therapeutic approaches for disease treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Jiwoong Kwon, Mohamed Saleh Elgawish, Sang-Hee Shim
Summary: Photobleaching is a major limitation in super-resolution microscopy, but it can be overcome by developing new probes, chemical environments, and utilizing the on-off transitions of fluorescence. These efforts have the potential to enhance resolution and extend the observation time in super-resolution microscopy.
Article
Biochemistry & Molecular Biology
Marina Martinez-Carmona, Fernando Lucas-Ruiz, Alejandro Gallego-Ortega, Caridad Galindo-Romero, Maria Norte-Munoz, Maria Jose Gonzalez-Riquelme, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: Ly6c can serve as a specific marker of mouse vasculature, particularly of the retinal vascular plexuses. In injured retinas, Ly6c expression increases, correlating with vascular formation and classical monocyte/macrophage activation. This study provides a new tool for qualitative and quantitative assessment of vascular changes in health and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Zhaoshuai Gao, Pei Wu, Lixin Yin, Bin Kang, Hong-Yuan Chen, Jing-Juan Xu
Summary: This study utilized the nonlinear plasmonic scattering response to achieve single-wavelength super-resolution imaging, successfully implementing this method on a custom-built system to achieve higher resolution than traditional methods.
CHEMICAL COMMUNICATIONS
(2021)
Review
Cardiac & Cardiovascular Systems
Floriana Maria Farina, Christian Weber, Donato Santovito
Summary: Most of our genome is transcribed into ncRNAs, including miRNAs, lncRNAs, circRNAs, etc., which play important roles in regulating gene expression at various levels. They are involved in chromatin remodeling, transcriptional regulation, mRNA splicing, stability, and decay, and ultimately affect physiological and pathological mechanisms in vascular biology and atherosclerosis. Recent research has revealed unexpected findings, expanding the functional repertoire of ncRNAs beyond gene regulation and highlighting an additional layer of biological control. This review discusses emerging aspects of ncRNA biology, their implications for vascular biology and atherosclerosis, and possible translational applications.
Article
Hematology
Myrthe E. Reiche, Kikkie Poels, Laura A. Bosmans, Winnie G. Vos, Claudia M. van Tiel, Marion J. J. Gijbels, Suzanne A. B. M. Aarts, Myrthe den Toom, Linda Beckers, Christian Weber, Dorothee Atzler, Patrick C. N. Rensen, Sander Kooijman, Esther Lutgens
Summary: The CD40-CD40L dyad has an important role in chronic inflammatory diseases associated with aging. This study found that CD40 is also present on adipocytes. Deficiency of adipocyte CD40 leads to a decrease in bone marrow hematopoietic stem cells, increased T-cell activation, and elevated corticosterone levels, particularly with age. However, adipocyte CD40 deficiency also reduces atherosclerosis burden and ameliorates diet-induced obesity.
Article
Microbiology
Linda Dengler, Julia Meier, Andreas Klingl, Laura Nissl, Annett Bellack, Dina Grohmann, Reinhard Rachel, Harald Huber
Summary: A novel interdomain consortium consisting of a methanogenic Archaeon and a sulfate-reducing bacterium was discovered in an oil well in Cahuita National Park, Costa Rica. Both organisms can be cultured separately or together. The methanogenic cells produce CH4 exclusively from H2/CO2, while the sulfate-reducing partner can utilize various electron donors and acceptors. The two strains represent new species named Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
ARCHIVES OF MICROBIOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Christian Weber, Andreas J. R. Habenicht, Philipp von Hundelshausen
Summary: This review summarizes recent progress in the therapeutic targeting of inflammatory components in atherosclerosis. Novel strategies, such as interference with the CD40L-CD40 dyad and selective targeting of TRAFs, show potential in reducing atherosclerosis and plaque instability without immune side effects. Additionally, modulation of the chemokine system and disruption of the adventitial neuroimmune cardiovascular interfaces offer promising avenues for intervention beyond anti-inflammatory approaches.
EUROPEAN HEART JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Christine Krammer, Bishan Yang, Sabrina Reichl, Simon Besson-Girard, Hao Ji, Verena Bolini, Corinna Schulte, Heidi Noels, Kai Schlepckow, Georg Jocher, Georg Werner, Michael Willem, Omar El Bounkari, Aphrodite Kapurniotu, Ozgun Gokce, Christian Weber, Sarajo Mohanta, Jurgen Bernhagen
Summary: Atherosclerosis is a chronic inflammatory condition of our arteries and the underlying pathology of heart attacks and strokes. The role of the inflammatory cytokine MIF in the progression of atherosclerosis and its relationship with age and atherogenic cytokines are not fully understood.
Meeting Abstract
Biochemistry & Molecular Biology
Lea Strohm, Steffen Daub, Matthias Oelze, Marin Kuntic, Henning Ubbens, Veronique Klein, Imo E. Hoefer, Alex Von Kriegsheim, Hartmut Kleinert, Christian Weber, Dorothee Atzler, Philipp Wild, Thomas Muenzel, Christoph Knosalla, Esther Lutgens, Andreas Daiber
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Payel Sen, Bachuki Shashikadze, Florian Flenkenthaler, Esther Van de Kamp, Siyu Tian, Chen Meng, Michael Gigl, Thomas Froehlich, Daphne Merkus
Summary: Pulmonary vein stenosis (PVS) causes pulmonary hypertension (PH) by affecting flow and pressure in the pulmonary vasculature, leading to endothelial dysfunction and metabolic changes. Targeted therapy is recommended to relieve pressure and reverse flow-related changes in this type of PH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Bachuki Shashikadze, Sophie Franzmeier, Isabel Hofmann, Martin Kraetzl, Florian Flenkenthaler, Andreas Blutke, Thomas Froehlich, Eckhard Wolf, Arne Hinrichs
Summary: This study investigated the histomorphological and molecular alterations associated with growth hormone receptor deficiency (GHRD). The results showed that GHRD pigs had reduced body weights, decreased pituitary volumes, and reduced volume density of GH-storing granules in somatotroph cells. Proteomic analysis revealed changes in protein expression related to protein production, transport, and endoplasmic reticulum (ER) stress. These findings provide insights into the molecular and histological changes caused by GHRD.
JOURNAL OF NEUROENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Ken Ikeuchi, Nives Ivic, Robert Buschauer, Jingdong Cheng, Thomas Froehlich, Yoshitaka Matsuo, Otto Berninghausen, Toshifumi Inada, Thomas Becker, Roland Beckmann
Summary: This study reveals the impact of Otu2-driven deubiquitylation of ribosomal protein eS7 on translational efficiency and provides insights into its role in translation reset. The authors found that eS7 in actively translating 80S ribosomes is monoubiquitinated by the E3 ligase Not4 and deubiquitinated by Otu2 upon ribosomal subunit recycling. Cryo-electron microscopy analysis showed that Otu2 mainly binds to the 40S subunit surface at sites not occupied by other factors.
NATURE COMMUNICATIONS
(2023)
Article
Environmental Sciences
Federica Lamberto, Bachuki Shashikadze, Radwa Elkhateib, Salvo Danilo Lombardo, Alex Horanszky, Andrea Balogh, Kornel Kistamas, Melinda Zana, Joerg Menche, Thomas Froehlich, Andras Dinnyes
Summary: This study investigated the effects of environmentally relevant doses of Bisphenol A (BPA) on developing cardiomyocytes using a human induced pluripotent stem cell (hiPSC)-derived model. The results showed that long-term treatment with low doses of BPA interferes with hiPSC-CMs functionality and alters the surrounding cellular environment, leading to potential diseases. This study contributes to the understanding of BPA effects on developing human foetal cardiomyocytes and provides a suitable model for environmental chemical hazard and risk assessment.
ENVIRONMENTAL POLLUTION
(2023)
Review
Biochemistry & Molecular Biology
Xavier Blanchet, Christian Weber, Philipp von Hundelshausen
Summary: Chemoattractant cytokines or chemokines are involved in gradient formation and cell recruitment. They interact with their receptors, glycosaminoglycans, and other soluble effector proteins to form complexes. Chemokine dimerization changes their structure and confers additional biological activities. This review provides an overview of chemokine functionality and their interactions with other proteins, as well as potential therapeutic tools targeting chemokine multimeric structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Michael Stirm, Bachuki Shashikadze, Andreas Blutke, Elisabeth Kemter, Andreas Lange, Jan B. Stoeckl, Florian Jaudas, Laeticia Laane, Mayuko Kurome, Barbara Kessler, Valeri Zakhartchenko, Andrea Baehr, Nikolai Klymiuk, Hiroshi Nagashima, Maggie C. Walter, Wolfgang Wurst, Christian Kupatt, Thomas Froehlich, Eckhard Wolf
Summary: Skipping DMD exon 51 can restore dystrophin expression and improve cardiac function in DMD patients and animal models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Developmental Biology
Pia Sessenhausen, Karolina M. Caban, Nicole Kreitmair, Mirko Peitzsch, Jan B. Stoeckl, Marie C. Meinsohn, David Pepin, Bastian Popper, Thomas Froehlich, Artur Mayerhofer
Summary: The nicotinic acetylcholine receptor alpha 7 (nAChRa7), encoded by Chrna7, is expressed in various ovarian cells and plays a role in the local regulation of the ovary.
Article
Multidisciplinary Sciences
Julian U. G. Wagner, Lukas S. Tombor, Pedro Felipe Malacarne, Lisa-Maria Kettenhausen, Josefine Panthel, Haris Kujundzic, Nivethitha Manickam, Katja Schmitz, Maria Cipca, Kathrin A. Stilz, Ariane Fischer, Marion Muhly-Reinholz, Wesley T. Abplanalp, David John, Sarajo K. Mohanta, Christian Weber, Andreas J. R. Habenicht, Giulia K. Buchmann, Stephan Angendohr, Ehsan Amin, Katharina Scherschel, Nikolaj Kloecker, Malte Kelm, Dominik Schuettler, Sebastian Clauss, Stefan Guenther, Thomas Boettger, Thomas Braun, Christian Baer, Minh-Duc Pham, Jaya Krishnan, Susanne Hille, Oliver J. Mueller, Tarik Bozoglu, Christian Kupatt, Eleonora Nardini, Selma Osmanagic-Myers, Christian Meyer, Andreas M. Zeiher, Ralf P. Brandes, Guillermo Luxan, Stefanie Dimmeler
Summary: Aging reduces nerve density in the heart and dysregulates vascular-derived neuroregulatory genes, leading to impaired cardiovascular health.
Article
Developmental Biology
Katja Eubler, Karolina M. Caban, Gregory A. Dissen, Ulrike Berg, Dieter Berg, Carola Herrmann, Nicole Kreitmair, Astrid Tiefenbacher, Thomas Frohlich, Artur Mayerhofer
Summary: This study discovered the expression of TRPV2 in the ovary and its involvement in inflammatory and immunological processes. The TRPV2 agonist CBD was found to induce Ca2+ fluxes and the production of inflammatory factors, as well as interfere with progesterone synthesis and alter the proteome and secretome.
MOLECULAR HUMAN REPRODUCTION
(2023)