Article
Biotechnology & Applied Microbiology
Li Wang, Chenhui Yang, Qianru Shan, Miao Zhao, Juanjuan Yu, Yong -Fang Li
Summary: The study investigated the effects of N6-methyladenosine (m6A) modification on rice growth and development. The researchers analyzed the m6A methylation patterns in rice panicles and flag leaves at different stages and found that the global m6A level varied significantly among tissues. They also discovered that tissue-specific methylated genes showed lower levels of m6A modification and expression, with a preference for m6A deposition in the coding sequence region. The study concluded that m6A modification influences tissue function by regulating gene expression, providing valuable insights into m6A modifications in rice.
Article
Biochemistry & Molecular Biology
Xue-Jiao You, Shan Zhang, Juan-Juan Chen, Feng Tang, Jingang He, Jie Wang, Chu-Bo Qi, Yu-Qi Feng, Bi-Feng Yuan
Summary: This study identified a novel adenosine dual methylation modification, m(1,6)A, in tRNAs of living organisms, prevalent in mammalian cells and tissues. The research also demonstrated that m(1,6)A could be catalyzed by TRMT6/61A and demethylated by ALKBH3.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Genetics & Heredity
Shu Fang, Bo Peng, Yanan Wen, Jingjing Yang, Hao Wang, Ziwei Wang, Kun Qian, Yan Wei, Yifan Jiao, Chunji Gao, Liping Dou
Summary: This study found that abnormal m(6)A modification plays an important role in Adriamycin-resistant AML. The analysis of sequencing data from HL60 and HL60/ADR cell lines revealed an elevated m(6)A level in the resistant cells, which was associated with multiple signaling pathways.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Qiang Luo, Jiezhen Mo, Hao Chen, Zetao Hu, Baihui Wang, Jiabing Wu, Ziyu Liang, Wenhao Xie, Kangxi Du, Maolin Peng, Yingping Li, Tianyang Li, Yangyi Zhang, Xiaoyan Shi, Wen-Hui Shen, Yang Shi, Aiwu Dong, Hailin Wang, Jinbiao Ma
Summary: This study reports the function of Arabidopsis METTL4 as a U2 snRNA methyltransferase that regulates flowering time by catalyzing N-6-2'-O-dimethyladenosine (m(6)Am) methylation in vivo. In vitro experiments show that METTL4 specifically catalyzes N-6-methylation of 2'-O-methyladenosine (Am) within single-stranded RNA. Structural analysis reveals the substrate binding site and catalytic center of METTL4, providing insights into the mechanism of N-6-adenosine methylation.
NATURE COMMUNICATIONS
(2022)
Review
Genetics & Heredity
Sadaruddin Chachar, Jingrong Liu, Pingxian Zhang, Adeel Riaz, Changfei Guan, Shuyuan Liu
Summary: Studies have shown that DNA 6mA modification plays a significant role in plants, particularly activating gene expression in model plants. However, the occurrence and importance of 6mA in non-model crops remain less understood, presenting an area for further research.
FRONTIERS IN GENETICS
(2021)
Article
Medicine, General & Internal
Zhiqiang Qiu, Xuanyang Bai, Xinye Han, Peishen Wang, Xiang Wang, Yuxia Lv, Yihua An
Summary: This study identified 16 significant RNA N6-methyladenosine (m6A) regulators involved in Alzheimer's disease (AD) and developed a nomogram model for predicting the prevalence of AD. The study also revealed distinct m6A patterns and gene patterns in AD, demonstrating their prognostic and risk assessment significance.
Article
Multidisciplinary Sciences
Shengyong Yu, Chunhua Zhou, Jiangping He, Zhaokai Yao, Xingnan Huang, Bowen Rong, Hong Zhu, Shijie Wang, Shuyan Chen, Xialian Wang, Baomei Cai, Guoqing Zhao, Yuhan Chen, Lizhan Xiao, He Liu, Yue Qin, Jing Guo, Haokaifeng Wu, Zhen Zhang, Man Zhang, Xiaoyang Zhao, Fei Lan, Yixuan Wang, Jiekai Chen, Shangtao Cao, Duanqing Pei, Jing Liu
Summary: This study utilizes single-cell RNA sequencing to examine the transition process of mouse stem cells and uncovers an alternative route from primed to naive pluripotency. The researchers discover that the transition goes through a primordial germ cell-like intermediate state. Furthermore, they elucidate the molecular mechanisms involved in this process, including the role of DOT1L, Gata2, and Prdm1/Blimp1.
NATURE COMMUNICATIONS
(2022)
Review
Medicine, Research & Experimental
Chang Liu, Zhe Yang, Rong Li, Yanju Wu, Ming Chi, Shuting Gao, Xun Sun, Xin Meng, Biao Wang
Summary: m6A modification, a common internal modification on RNA molecules, has been linked to various human diseases, making it a research hotspot. This modification plays a regulatory role in immune responses, affecting disease development.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Hong Seo Choi, Hyun Min Lee, Min Kyu Kim, Chun Jeih Ryu
Summary: This study investigates the difference between two states of human pluripotent stem cells and identifies heat shock protein 60 (HSP60) as a key regulator in maintaining pluripotency gene expression. The results show that HSP60 is downregulated during cell differentiation and its depletion leads to decreased expression of pluripotency genes.
Article
Microbiology
Miaoge Xue, Yuexiu Zhang, Haitao Wang, Elizabeth L. Kairis, Mijia Lu, Sadeem Ahmad, Zayed Attia, Olivia Harder, Zijie Zhang, Jiangbo Wei, Phylip Chen, Youling Gao, Mark E. Peeples, Amit Sharma, Prosper Boyaka, Chuan He, Sun Hur, Stefan Niewiesk, Jianrong Li
Summary: The study indicates that RSV lacking m(6)A methylation induces higher type I interferon response and is more immunogenic than wild-type RSV. This suggests that inhibition of RSV RNA m(6)A methylation can enhance innate immune responses and subsequently promote adaptive immunity against the virus.
Review
Oncology
Hongyuan Wan, Yanyan Feng, Junjie Wu, Lijie Zhu, Yuanyuan Mi
Summary: This article summarizes the recent findings on m(6)A regulation and its functions in prostate cancer, highlighting the presence of functional proteins within m(6)A in prostate cancer cells. The potential clinical value of m(6)A modification as an early diagnostic marker and treatment evaluation indicator in prostate cancer was also discussed.
MOLECULAR MEDICINE REPORTS
(2022)
Review
Pharmacology & Pharmacy
Yu Xia, Yanyan Zhang, Jie Huang, Bing Chen, Yanjiao Jiang, Zengxian Sun, Yun Liu
Summary: This article reviews the latest research advances in the field of m6A RNA methylation in pulmonary hypertension and explores its regulatory role in the development and progression of pulmonary hypertension.
Review
Cell Biology
Nan Wang, Fei Yao, Die Liu, Haibo Jiang, Xiaobo Xia, Siqi Xiong
Summary: N-6-methyladenosine (m(6)A) is the most abundant transcriptome modification in eukaryotic messenger RNA, playing crucial roles in regulating RNA functions and expressions, impacting physiological processes, tumorigenesis, viral infection, and ocular diseases such as uveal melanoma and diabetic retinopathy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Environmental Sciences
Yujie Wei, Xiaona Guo, Lifeng Li, Wenhua Xue, Longhao Wang, Chengxin Chen, Shilong Sun, Yaqi Yang, Wu Yao, Wei Wang, Jie Zhao, Xiaoran Duan
Summary: Polycyclic aromatic hydrocarbons (PAHs) are environmental toxicants that can cause a range of toxic reactions in humans. Long-term exposure to PAHs is associated with an increased incidence of cancer. The N6-methyladenosine (m6A) modification affects RNA metabolism and is linked to the development of various diseases. Abnormal expression of m6A-binding proteins is implicated in the malignant proliferation and metastasis of cancers. Environmental toxicants, including PAHs, can impact m6A methylation and protein expression, thereby promoting the occurrence and development of cancers. m6A may serve as a biomarker for early environmental exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Cell Biology
Le Zhang, Xin Zhao, Jing Wang, Yanwu Jin, Moxuan Gong, Yuyang Ye, Peilong Li
Summary: In this study, the role of METTL3-regulated N6-methyladenosine in modulating primiR-150 maturation during neuropathic pain progression was demonstrated. METTL3 was found to promote m6A methylation and inhibit NP, while its downregulation exacerbated the severity of NP. The METTL3/miR-150/BDNF pathway may serve as a promising therapeutic target for NP patients.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Li Chen, Ying-Chun Ouyang, Jian Li, Jing-Yi Qiao, Lin -Jian Gu, Zhen-Bo Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun
Summary: The study indicates that Septin 4 plays a crucial role in regulating the G2/M transition in mouse oocytes by controlling the accumulation of CCNB1. Depletion of Septin 4 leads to GV arrest and alters the expression level of CDC20, while there is no significant change in the expression level of CDH1.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiaqi Zhang, Wenbo Liu, Guangyue Li, Chengpeng Xu, Xiaoqing Nie, Dandan Qin, Qizhi Wang, Xukun Lu, Jianqiao Liu, Lei Li
Summary: Alternative splicing is an important mechanism for regulating organogenesis and fertility. BCAS2 plays a crucial role in mouse oocytes, participating in oocyte development through regulating splicing and influencing follicular development.
Article
Biochemistry & Molecular Biology
Wei Yue, Yue Wang, Tie-Gang Meng, Hong-Yong Zhang, Xin-Ran Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: This study investigates the role of outer kinetochore protein KNL1 in mouse oocytes and reveals its critical function in ensuring timely and accurate chromosome segregation during oocyte meiotic maturation.
Article
Developmental Biology
Ming-Zhe Dong, Ying-Chun Ouyang, Shi-Cai Gao, Xue-Shan Ma, Yi Hou, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Summary: PPP4C regulates the activity of PLK1 in early embryonic cells, which plays a crucial role in DNA damage repair.
Article
Biochemistry & Molecular Biology
Yujun Quan, Meijiao Wang, Chengpeng Xu, Xiaoxiao Wang, Yu Wu, Dandan Qin, Yuxuan Lin, Xukun Lu, Falong Lu, Lei Li
Summary: Mammalian early epiblasts undergo pluripotency transitions from naive to formative states, involving extensive transcriptome changes. The deadenylase Cnot8 of Ccr4-Not complex is found to play crucial roles in this transition. Knock out of Cnot8 leads to embryonic lethality in mice, but Cnot8 knockout embryonic stem cells (ESCs) can be established. Compared to normal ESCs, Cnot8 knockout cells exhibit highly expressed genes during their differentiation into the formative state.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Feng-Ze Yan, Ying-Chun Ouyang, Tie-Gang Meng, Hong-Yong Zhang, Wei Yue, Xin-Ran Zhang, Yue Xue, Zhen-Bo Wang, Qing-Yuan Sun
Summary: The study found that AZD1208 treatment has toxic effects on mouse oocytes, causing mitochondrial dysfunction and delayed spindle assembly.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Jian Li, Hao-Ya Chang, Zi-Yun Yi, Chun-Hui Zhang, Qing-Yuan Sun, Wei-Ping Qian
Summary: Studies on mammalian oocytes have shown that CDK2 activity plays a crucial role in the proper dynamics of spindle and chromosomes, with CDK2 inhibition leading to continuous SAC activation and subsequent inactivation of APC/C activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Yue Xue, Tie-Gang Meng, Ying-Chun Ouyang, Sai-Li Liu, Jia-Ni Guo, Zhen-Bo Wang, Heide Schatten, Chun-Ying Song, Xing-Ping Guo, Qing-Yuan Sun
Summary: This study found that Miro1 plays a crucial role in the maturation process of mouse oocytes by regulating mitochondrial homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Li Chen, Ying-Chun Ouyang, Lin-Jian Gu, Jia-Ni Guo, Zhi-Ming Han, Zhen-Bo Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun
Summary: This study demonstrates the critical role of Septin 9 in mouse oocyte meiotic cell cycle progression. Septin 9 regulates the kinetochore-microtubule connection and SAC protein localization on kinetochores, affecting the MI-AI transition as well as APC/C-CDC20 activity and CCNB1 degradation.
CELL PROLIFERATION
(2023)
Article
Cell Biology
Chao Li, Yong-Peng Tan, Xue-Shan Ma, Zhen-Bo Wang, Tie-Gang Meng, Qing-Yuan Sun
Summary: This study investigated the roles of CDT1 and MCM2 in oocyte meiotic maturation and early embryonic development. The results showed that CDT1 is the major regulator of DNA replication, while MCM2 has no effect on oocyte meiotic maturation. The degradation of CDT1 is essential for preventing DNA re-replication in zygotes.
CELL PROLIFERATION
(2023)
Article
Multidisciplinary Sciences
Cheng-Jie Zhou, Xing-Yue Wang, Yan-Hua Dong, Dong-Hui Wang, Zhe Han, Xiao-Jie Zhang, Qing-Yuan Sun, John Carroll, Cheng-Guang Liang
Summary: DRP1 is recruited to the kinetochore by CENP-F after nuclear envelope breakdown in mouse oocytes. Loss of DRP1 during different stages of the cell cycle results in premature chromosome segregation and abnormal degradation of cyclinB1 and securin. Additionally, DRP1 binding can restrain the E3 ligase activity of APC/C.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Tie-Gang Meng, Jia-Ni Guo, Liu Zhu, Yike Yin, Feng Wang, Zhi-Ming Han, Lei Lei, Xue-Shan Ma, Yue Xue, Wei Yue, Xiao-Qing Nie, Zheng-Hui Zhao, Hong-Yong Zhang, Si-Min Sun, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhenyu Ju, Xiang-Hong Ou, Zhen-Bo Wang, Catherine C. L. Wong, Zhonghan Li, Qing-Yuan Sun
Summary: The study reveals that NLRP14 is crucial for maintaining calcium oscillations and early embryonic development. Disruption of NLRP14 leads to impaired cytoplasmic function and calcium homeostasis due to altered mitochondrial distribution, morphology, and activity. The interaction between NLRP14 and the mitochondrial Na+/Ca2+ exchanger (NCLX) is essential for regulating calcium homeostasis and embryonic development.
Article
Oncology
Ang Li, Fei Li, Wei Song, Zi-Li Lei, Qian-Qian Sha, Shao-Yuan Liu, Chang-Yin Zhou, Xue Zhang, Xiao-Zhen Li, Heide Schatten, Teng Zhang, Qing-Yuan Sun, Xiang-Hong Ou
Summary: This study investigated the relationship between gut microbiota and oocyte quality decline caused by circadian rhythm disruption. The results showed that the decreased absorption capacity of vitamin D in the intestine, as a result of reduced levels of bile acids such as lithocholic acid (LCA) and increased abundance of Turicibacter, led to decreased oocyte quality and embryonic development. The concentrations of anti-Mullerian hormone (AMH) hormone in blood and melatonin in follicular fluid were also reduced, contributing to the decline in oocyte quality and early embryonic development. Injection of vitamin D3 (VD3) or specific metabolites was able to rescue the oocyte quality and embryonic development. Overall, this study highlights the importance of gut microbiota regulation by microbial metabolites in improving ovary function.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Developmental Biology
Di Gao, Xin Wang, Ye-Lian Yan, Chao Li, Yong-Peng Tan, Qiu-Chen Liu, Meng-Ya Zhang, Jian Zhang, Qing-Yuan Sun, Zu-Bing Cao, Yun-Hai Zhang
Summary: circKDM5B regulates porcine early embryo development by mediating miR-128. Knockdown of circKDM5B leads to reduced blastocyst formation, decreased cell numbers, impaired tight junction assembly, and disturbed paracellular sealing within the TE epithelium. Injection of miR-128 inhibitor rescues the early development of circKDM5B knockdown embryos.
MOLECULAR HUMAN REPRODUCTION
(2023)