Article
Medicine, Research & Experimental
Maciej Korczak, Piotr Roszkowski, Weronika Skowronska, Klaudia M. Zoldak, Dominik Popowski, Sebastian Granica, Jakub P. Piwowarski
Summary: Urolithin A is a promising health-boosting agent that promotes mitophagy, improves skeletal muscle function, and suppresses the inflammatory response. By synthesizing new derivatives of urolithin conjugated with nonsteroidal anti-inflammatory drugs, its activity can be enhanced and intestinal metabolism can be improved.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Agriculture, Multidisciplinary
Yunika Mayangsari, Natsumi Sugimachi, Wenxi Xu, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: This study demonstrated that 3,5,7,3',4'- pentamethoxyflavone (PMF) can enhance the intestinal tight junction (TJ) barrier integrity by regulating the transcription of TJ proteins, such as occludin, claudin-3, and claudin-4.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Dan Su, Jiawen Lu, Chunchao Nie, Ziyan Guo, Chang Li, Qiang Yu, Jianhua Xie, Yi Chen
Summary: This study aimed to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability, decreased TEER values, and increased LY permeabilization, LDH release, and ROS levels. Furthermore, AA and OTA treatment led to increased levels of IL-1 beta, IL-6, and TNF-alpha, while IL-10 levels decreased. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of tight junction proteins.
Article
Entomology
Kyong Kim, Eun-Young Park, Dong-Jae Baek, Se-Eun Jang, Yoon-Sin Oh
Summary: Increased tight junction permeability and overproduction of proinflammatory cytokines are crucial in inflammatory bowel disease (IBD). This study evaluated the anti-inflammatory effects of Gryllus bimaculatus extract (AE-GBE) against lipopolysaccharide (LPS)-treated Caco-2 cells, showing that AE-GBE can protect epithelial cells by increasing tight junction proteins and reducing inflammatory mediators.
Article
Polymer Science
Abdul Rahim Chethikkattuveli Salih, Kinam Hyun, Arun Asif, Afaque Manzoor Soomro, Hafiz Muhammad Umer Farooqi, Young Su Kim, Kyung Hwan Kim, Jae Wook Lee, Dongeun Huh, Kyung Hyun Choi
Summary: Cellular microenvironment is crucial for tissue growth patterns and can be influenced by ECM. Hepatic microphysiological systems emulate human liver physiology for drug screening, and standardized ECM coatings on microfluidic devices may enhance organ-specific emulation. Optimizing surface coatings of different ECM types can improve tissue development, with image processing and TEER sensors assisting in tissue analysis.
Article
Cell Biology
Smita Kapoor, Yogendra S. Padwad
Summary: Ulcerative colitis is an inflammatory bowel disease associated with an increased risk of colorectal cancer. This study investigated the anti-inflammatory potential of phloretin, a compound found in fruits like apple, pear, and strawberries, using an in vitro gut inflammation model. The findings suggest that phloretin has anti-inflammatory effects and may be beneficial in preventing colitis and colorectal cancer.
CELLULAR IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Ying-Kai Hung, Shang-Tse Ho, Ching-Yun Kuo, Ming-Ju Chen
Summary: The study demonstrated that velvet antler extracts could enhance the integrity of colonic epithelial cell barriers and promote repair through specific mechanisms. Future animal studies are needed for further confirmation.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Xiaoyu Bao, Jianping Wu
Summary: This study demonstrates the ability of IRW to prevent LPS-induced intestinal barrier dysfunction and inhibit inflammatory responses. IRW mitigates the LPS-induced reduction of intestinal barrier function by restoring the expression and cell surface distribution of the tight junction protein occludin. Furthermore, IRW shows LPS-neutralizing activity and inhibits the activation of NF-KB and MAPK signaling pathways.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Lijiao Kan, Edoardo Capuano, Vincenzo Fogliano, Ruud Verkerk, Jurriaan J. Mes, Monic M. M. Tomassen, Teresa Oliviero
Summary: The study showed that tea polyphenols have inhibitory effects on alpha-glucosidase activity in both rat intestinal enzymes and human Caco-2 cells, but the specific effects varied depending on the type of tea extract and the enzyme being targeted.
Article
Veterinary Sciences
Rosanna Marsella, Kim Ahrens, Rachel Wilkes
Summary: Skin barrier dysfunction is important in atopic dermatitis and may be related to differences in cell growth behavior rather than tight junction protein differences. Atopic keratinocytes have lower TEER than normal keratinocytes, indicating higher epithelial permeability.
VETERINARY SCIENCES
(2022)
Article
Food Science & Technology
Melissa Fanzaga, Carlotta Bollati, Giulia Ranaldi, Sabrina Sucato, Silvia Fustinoni, Gabriella Roda, Carmen Lammi
Summary: In recent years, there has been a growing interest in exploring alternative delivery systems for iron supplements to improve efficacy and reduce side effects. This study demonstrates the advantages of the Ferro Supremo formulation in improving iron absorption compared to standard FeSO4. The results show that Ferro Supremo can enter, accumulate, and be transported by intestinal cells more efficiently than FeSO4, making it a valuable choice for improving iron deficiency.
Article
Biochemistry & Molecular Biology
Jun Sung Park, Kyonghwan Choe, Amjad Khan, Myeung Hoon Jo, Hyun Young Park, Min Hwa Kang, Tae Ju Park, Myeong Ok Kim
Summary: The aim of this study was to establish a functional in vitro co-cultured BBB model to investigate BBB-related physiological conditions. A co-cultured model consisting of brain-derived endothelial and astrocyte cells was successfully established on transwell membranes. The co-cultured model showed effective barrier properties and enhanced expression of tight junction proteins. Under disease conditions, the co-cultured model mimicked BBB damages. This in vitro BBB model can be a useful tool for studying BBB-related pathological and physiological processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Thanyaporn Srimahaeak, Fernanda Bianchi, Ondrej Chlumsky, Nadja Larsen, Lene Jespersen
Summary: The study found that citrus pectins with high and moderate degrees of esterification can enhance adhesion of L. fermentum PCC, while those with low DE can reduce it. Combining with pectins significantly increased TEER, indicating a synergistic action of bacteria and pectins that contributes to the health of the epithelial barrier.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biochemistry & Molecular Biology
Aili Li, Chao Zhang, Houyu Chi, Xueting Han, Yiming Ma, Jie Zheng, Chuan Liu, Chun Li
Summary: 2'-fucosyllactose (2'-FL) enhances the adhesion ability of Lactobacillus rhamnosus KLDS 8001 (KLDS 8001) to Caco-2 cells and improves its anti-adhesive effect against pathogenic bacteria. It also reduces lipopolysaccharide (LPS)-induced damage, promotes repair, and upregulates the expression of adhesion related proteins.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Jae Hoon Lee, Sera Kim, Min-Cheol Kang, Yong Gi Chun, Bum-Keun Kim, Kyung-Mo Song
Summary: This study confirms the protective effect of four nut oils on the damaged intestinal epithelial barrier. Nut oils improve barrier function, inhibit inflammatory response and cytokine production, and enhance the expression and distribution of tight junction proteins in damaged cells.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemical Research Methods
Bas W. M. van Balkom, Hendrik Gremmels, Bernd Giebel, Sai Kiang Lim
Article
Peripheral Vascular Disease
Hendrik Gremmels, Femke C. C. van Rhijn-Brouwer, Diana A. Papazova, Joost O. Fledderus, Martin Teraa, Marianne C. Verhaar
Article
Multidisciplinary Sciences
Hendrik Gremmels, Martin Teraa, Saskia C. A. de Jager, Gerard Pasterkamp, Gert J. de Borst, Marianne C. Verhaar
SCIENTIFIC REPORTS
(2019)
Article
Surgery
Joep G. J. Wijnand, Ian D. van Koeverden, Martin Teraa, Marlon I. Spreen, Willem P. T. M. Mali, Hans van Overhagen, Gerard Pasterkamp, Gert J. de Borst, Michael S. Conte, Hendrik Gremmels, Marianne C. Verhaar
JOURNAL OF VASCULAR SURGERY
(2020)
Article
Peripheral Vascular Disease
Judith Kooiman, Fieke Terstappen, Lilian van Wagensveld, Arie Franx, Kimberley E. Wever, Tessa J. Roseboom, Jaap A. Joles, Hendrik Gremmels, A. Titia Lely
Article
Cell & Tissue Engineering
Femke C. C. van Rhijn-Brouwer, Bas W. M. van Balkom, Diana A. Papazova, Dienty H. M. Hazenbrink, Anke J. Meijer, Isaac Brete, Vidalmar Briceno, Arjan D. van Zuilen, Raechel J. Toorop, Joost O. Fledderus, Hendrik Gremmels, Marianne C. Verhaar
STEM CELLS INTERNATIONAL
(2019)
Article
Surgery
Maarten C. Verwer, Joep G. J. Wijnand, Martin Teraa, Hendrik Gremmels, Jessica P. Simons, Michael S. Conte, Marianne C. Verhaar, Gert J. de Borst
JOURNAL OF VASCULAR SURGERY
(2020)
Letter
Infectious Diseases
Steven Thijsen, Michiel Heron, Hendrik Gremmels, Robert van der Kieft, Chantal Reusken, Kristin Kremer, Gijs Limonard, Ailko Bossink
JOURNAL OF INFECTION
(2020)
Review
Nutrition & Dietetics
Fieke Terstappen, Angela J. C. Tol, Hendrik Gremmels, Kimberley E. Wever, Nina D. Paauw, Jaap A. Joles, Eline M. van der Beek, A. Titia Lely
Article
Polymer Science
Paul J. Besseling, Tristan Mes, Anton W. Bosman, Joris W. Peeters, Henk M. Janssen, Maarten H. Bakker, Joost O. Fledderus, Martin Teraa, Marianne C. Verhaar, Hendrik Gremmels, Patricia Y. W. Dankers
Summary: Supramolecular biomaterials based on ureido-pyrimidinone (UPy) moieties are versatile polymer materials that can be tailored to various applications with good biocompatibility and solubility, showing potential for use in regenerative medicine and biomedical fields.
JOURNAL OF POLYMER SCIENCE
(2021)
Meeting Abstract
Obstetrics & Gynecology
Judith Kooiman, Fieke Terstappen, Lilian van Wagensveld, Arie Franx, Kimberley E. Wever, Tessa J. Roseboom, Jaap A. Joles, Hendrik Gremmels, Titia A. Lely
REPRODUCTIVE SCIENCES
(2020)
Article
Chemistry, Medicinal
Hamed Janbazacyabar, Jeroen van Bergenhenegouwen, Kim A. T. Verheijden, Thea Leusink-Muis, Ardy van Helvoort, Johan Garssen, Gert Folkerts, Saskia Braber
Article
Transplantation
Hendrik Gremmels, Olivier G. de Jong, Raechel J. Toorop, Laura Michielsen, Arjan D. van Zuilen, Alexander V. Vlassov, Marianne C. Verhaar, Bas W. M. van Balkom
TRANSPLANTATION DIRECT
(2019)
Meeting Abstract
Obstetrics & Gynecology
Fieke Terstappen, Nina D. Paauw, Wessel Ganzevoort, Jaap A. Joles, Hendrik Gremmels, Anna T. Lely
REPRODUCTIVE SCIENCES
(2018)
Article
Endocrinology & Metabolism
Nordin M. J. Hanssen, Martin Teraa, Jean L. J. M. Scheijen, Marjo Van de Waarenburg, Hendrik Gremmels, Coen D. A. Stehouwer, Marianne C. Verhaar, Casper G. Schalkwijk
Summary: The study revealed that plasma MGO levels are associated with adverse outcomes in SLI, showing a strong correlation with mortality and amputation rates in patients.