Article
Pharmacology & Pharmacy
Juan Ignacio Elio Mariangelo, Carlos Alfredo Valverde, Leticia Vittone, Matilde Said, Cecilia Mundina-Weilenmann
Summary: This study found that blocking ER calcium efflux via the translocon can suppress ER stress and improve the mechanical performance and diastolic calcium handling of stunned myocardium. Therefore, modulation of translocon permeability may be a therapeutic approach to treat the functional abnormalities caused by ischemia-reperfusion injury.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Tresa M. Elias, Edward B. B. Brown Jr, Edward B. B. Brown III
Summary: Multi-photon fluorescence recovery after photobleaching (MPFRAP) is a nonlinear microscopy technique used to measure the diffusion coefficient of fluorescently tagged molecules in solution. This study proposes an MPFRAP fitting model that accounts for shear stress in laminar flow, making it a more applicable technique for in vitro and in vivo studies involving diffusion.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Cell Biology
Michelle Joy-Immediato, Manuel J. Ramirez, Mauricio Cerda, Yusuke Toyama, Andrea Ravasio, Pakorn Kanchanawong, Cristina Bertocchi
Summary: Cadherin-mediated adhesions are adhesive complexes that connect neighboring cells in a tissue. The extension of the ER to these adhesions is tension dependent and cadherin-type specific. The structural organization of the ER/microtubule network can affect the localization of ER-bound PTP1B at these adhesions, potentially modulating the mechanotransduction of adherens junctions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Hua Wei, Xiaochan Tang, Qingxian Chen, Tao Yue, Baoli Dong
Summary: In this study, an ER-targeting fluorescent probe was developed to detect viscosity changes in the endoplasmic reticulum (ER) during ferroptosis. The results showed that erastin-induced ferroptosis led to an increase in ER viscosity, which could be inhibited by Fer-1 and VE.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Hui Wang, Xiaoting Zhang, Mingyan Dong, Wei Zhang, Wen Zhang, Ping Li, Bo Tang
Summary: Rheumatoid arthritis is a chronic inflammatory disease that causes extreme joint pain, and current therapies cannot provide relief for many patients. The development of novel therapeutic drugs is a major challenge for RA treatment. Research shows that endoplasmic reticulum stress is involved in RA pathogenesis, and ER pH could potentially become a new evaluation parameter.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Maxim M. Perfilov, Elvira R. Zaitseva, Alexander Yu Smirnov, Andrey A. Mikhaylov, Nadezhda S. Baleeva, Ivan N. Myasnyanko, Alexander S. Mishin, Mikhail S. Baranov
Summary: This paper presents a new series of arylidene-imidazolones with a structural motif similar to the Green Fluorescent Protein chromophore, showing significant solvent-dependent variation in fluorescence quantum yield. Structure-activity patterns identified in this study allowed for the creation of a range of fluorogenic dyes with different spectral maxima positions and Stokes shifts. The developed dyes are cell-permeable and can selectively stain the endoplasmic reticulum in living eukaryotic cells.
Article
Biochemistry & Molecular Biology
Maxim M. Perfilov, Elvira R. Zaitseva, Nadezhda S. Baleeva, Vadim S. Kublitski, Alexander Yu. Smirnov, Yulia A. Bogdanova, Svetlana A. Krasnova, Ivan N. Myasnyanko, Alexander S. Mishin, Mikhail S. Baranov
Summary: In this work, we demonstrated that the addition of a trifluoromethyl group to the meta-position of arylidene imidazolones (GFP chromophore core) significantly enhances their fluorescence intensity in nonpolar and aprotic solvents. The solvent-dependent modulation of fluorescence intensity allows these compounds to be used as fluorescent polarity sensors. Specifically, we identified a compound suitable for selective labeling of the endoplasmic reticulum in living cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Marine & Freshwater Biology
Nan Zhang, Kang Li, Bin-Bin Xie, Xiu-Lan Chen, Bai-Cheng Zhou, Hai-Nan Su, Yu-Zhong Zhang
Summary: The study found that fluorescence recovery of PBS in four different phenotypes and PBS compositions of cyanobacteria was observed in wholly photobleached cells, where the contribution of PBS diffusion to the fluorescence recovery was not possible. Additionally, fluorescence recovered in isolated PBSs and PBS-thylakoid membranes after photobleaching further demonstrated the intrinsic photoprocess nature of fluorescence recovery.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Lizhou Yue, Huawei Huang, Wenhui Song, Weiying Lin
Summary: In this study, a near-infrared fluorescent probe targeted to the endoplasmic reticulum (ER) was developed for highly selective and sensitive detection of sulfur dioxide (SO2). The probe successfully allowed imaging of SO2 in living systems and revealed the role of SO2 in ER-associated diseases.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Arshag D. Mooradian, Michael J. Haas
Summary: Despite advances in cardiovascular disease prevention, there is still a significant risk of adverse cardiovascular events. Reducing cellular stresses, particularly inflammatory stress and endoplasmic reticulum stress, may further reduce cardiovascular disease risk.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Minjie Zhang, Xin Jin, Meng Gao, Yunjiao Zhang, Ben Zhong Tang
Summary: A sensitive self-reporting immunogenic cell death (ICD) inducer targeting endoplasmic reticulum (ER) stress is developed. The inducer can rapidly generate reactive oxygen species (ROS) and unfolded protein response process, leading to the release of damage-associated molecular patterns and efficient dendritic cell maturation. The fluorescence signal of the inducer allows real-time monitoring of ER accumulation and stress induction.
ADVANCED MATERIALS
(2022)
Article
Environmental Sciences
Junjun Peng, Xueyan Dai, Huiqin Fan, Chenghong Xing, Yu Zhuang, Xiaona Gao, Huabin Cao, Guoliang Hu, Fan Yang
Summary: Copper (Cu) at high levels can cause harm to the host physiology, specifically resulting in nephrotoxicity, although the exact mechanism remains unclear. This study explored the relationship between mitochondria-associated ER membranes (MAM) and Cu-induced kidney mitochondrial dysfunction and ER stress. The findings revealed that excessive Cu disrupted MAM integrity, leading to MAM dysfunction, mitochondrial dysfunction, and ER stress, contributing to Cu-induced nephrotoxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Cell Biology
Jennifer Flournoy, Shahad Ashkanani, Yun Chen
Summary: Biophysical and biochemical cues cooperate to regulate angiogenesis, guiding its process during development, wound healing, and tumor progression. The mechanical microenvironment, including stiffness, ligand availability, and topography, plays a crucial role in modulating angiogenesis-related signaling pathways. Additionally, mechanical perturbations such as stretching and fluid shearing can acutely alter angiogenesis-related signal transduction, leading to gene expression changes. Tissue engineering-based approaches are also discussed for studying angiogenesis, along with proposed future directions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinxuan Li, Qianqian Xiao, Qianqian Tan, Junpeng Chen, Lingyu Sun, Xiao Chen, Ziyu Chu, Hongxia Wu, Zhenzhao Zhang, Hongmei Li, Xiaomin Zhao, Xiao Zhang
Summary: MORN proteins play a crucial role in the cytoskeletal structure of eukaryotes and are essential for the close arrangement of the endoplasmic reticulum and plasma membrane. Through adjacent protein labeling techniques, we identified a network of interactions involving TgMORN2 and endoplasmic reticulum stress-related proteins. The pathogenicity of the KO-TgMORN2 strain was significantly reduced under tunicamycin-induced ER stress. Overall, TgMORN2 functions in ER stress and provides a basis for further research on the function of MORN proteins in Toxoplasma gondii.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sylwia Rzeszotek, Agnieszka Kolasa, Anna Pilutin, Kamila Misiakiewicz-Has, Katarzyna Sielatycka, Barbara Wiszniewska
Summary: Finasteride causes androgen imbalance, leading to ERet stress and liver disorders. UPR and ERAD are two pathways involved in cellular protein quality control in ERet. Paternal androgen imbalance may affect liver function in offspring through ERet stress, and oxidative stress is also a factor affecting ERet function.