Review
Biochemistry & Molecular Biology
Kasturi Ganguly, Uday Kishore, Taruna Madan
Summary: C-type lectin receptors (CLRs) are crucial in regulating physiological functions, recognizing pathogen-associated patterns, and initiating downstream signaling cascades. MicroRNAs (miRNAs) play a key role in regulating CLR expression, and the balance between miRNAs and CLRs is essential in maintaining cellular homeostasis and immunity. Dysregulations in the miRNA-CLR axes may lead to tumorigenesis or inflammatory diseases.
Article
Polymer Science
Haibo Feng, Xiaonong Yang, Linzi Zhang, Qianqian Liu, Yangyang Feng, Daiyan Wu, Yunjie Liu, Jie Yang
Summary: The MAN-CS-OVA-PLGA-MPs delivery system shows potential for enhancing immune responses by activating T cells, promoting dendritic cell maturation, and improving antigen presentation efficiency. This targeting system not only boosts DC activity, but also significantly impacts cytokine production and specific antibody levels in mice.
Review
Rheumatology
Murad Alahdal, Hui Zhang, Rongxiang Huang, Wei Sun, Zhiqin Deng, Li Duan, Hongwei Ouyang, Daping Wang
Summary: Dendritic cells have been found to play a potential role in the pathogenesis of osteoarthritis, with studies showing increased expression of toll-like receptors and secretion of inflammatory cytokines by DCs derived from OA patients. Strategies involving tolerogenic plasmacytoid DCs injections are suggested for treating OA inflammations.
Article
Nanoscience & Nanotechnology
Marina Bekic, Milos Vasiljevic, Dusica Stojanovic, Vanja Kokol, Dusan Mihajlovic, Dragana Vucevic, Petar Uskokovic, Miodrag Colic, Sergej Tomic
Summary: The purpose of this study was to investigate the immunomodulatory effects of phosphonates conjugated with cellulose nanocrystals (CNCs) in human cells. The results showed that the delivery of 3-AminoPropylphosphonic Acid (ApA) via CNCs induced potent dendritic cell-mediated Th1 polarization, which could have potential application in tumor therapy.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Ummey Jannatun Nahar, Istvan Toth, Mariusz Skwarczynski
Summary: This article discusses the use of adjuvants and vaccine delivery systems to enhance vaccine efficacy. It focuses on the role of mannose receptors in antigen recognition by antigen presenting cells (APCs). The production strategies and effectiveness of mannosylated vaccines are examined, considering factors such as mannose valency, density, and routes of administration. The advantages, disadvantages, and future directions of mannosylation strategy are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biology
Crystal C. C. Uwazie, Tyler U. U. Faircloth, Rhett N. N. Parr, Yenamala U. U. Reddy, Peiman Hematti, Devi Rajan, Raghavan Chinnadurai
Summary: In this study, the researchers investigated the effects of human bone-marrow-derived MSCs on peripheral blood dendritic cell responses. They found that plasmacytoid dendritic cells were not significantly affected by MSCs, but myeloid dendritic cells showed increased maturation. The study also identified the secretory factors associated with MSC-mediated upregulation of myeloid dendritic cell maturation. These findings suggest that circulating dendritic cell subsets may serve as potency biomarkers in MSC therapy.
Article
Pharmacology & Pharmacy
Yunting Zhang, Min Jiang, Guangsheng Du, Xiaofang Zhong, Chunting He, Ming Qin, Yingying Hou, Rong Liu, Xun Sun
Summary: The rise of nanotechnology has brought new possibilities for cancer immunotherapy. However, most nanovaccines made with nanomaterials face challenges such as low drug load, unpredictable metabolism, and potential toxicity, which hinder their clinical applications. In this study, a self-assembled nanovaccine was developed using a modified antigen. It incorporated a mannose monomer that targets dendritic cells and a mevalonate pathway inhibitor called zoledronic acid (Zol) to enhance immune response. The nanovaccine demonstrated successful transport to lymph nodes, high internalization by dendritic cells, increased expression of costimulatory molecules, and effective antigen presentation. These superior properties led to significantly higher levels of serum antibodies, stronger cytotoxic T lymphocyte responses, and increased secretion of IFN-gamma compared to free antigen and adjuvants. In vivo experiments showed that the nanovaccine suppressed tumor growth and prolonged the survival of mice with EG7-OVA tumors. These findings suggest that our self-assembled nanovaccine holds promise for immune potentiation and cancer immunotherapy.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Biochemistry & Molecular Biology
Shengwei Wang, Juncheng Chen, Chao Feng, Haibo Lan, Jucai Xu, Ruili Yang, Congfa Li, Wu Li
Summary: An active polysaccharide (LP) from longan was purified and characterized. LP consists of galactose and glucose in a molar ratio of 1.5:98.5, with a molecular weight of 4.67 x 107 g/mol. The main backbone of LP is T-alpha-D-Glcp-[(1-* 6)-alpha-D-Glcp-(1-* 6)-alpha-D-Glcp]n. After simulated gastrointestinal digestion, the molecular weight distribution, monosaccharide composition, and major glycosidic bonds of LP were not significantly changed. LP and digested LP (DLP) reduced phagocytosis and promoted IL-10 and IL-12 secretion of dendritic cells. Additionally, there was no significant difference in the effects of LP and DLP on activating dendritic cells. This study helps to illuminate the potential mode of immunomodulatory action of longan polysaccharides in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Abigail K. Grootveld, Wunna Kyaw, Veera Panova, Angelica W. Y. Lau, Emily Ashwin, Guillaume Seuzaret, Rama Dhenni, Nayan Deger Bhattacharyya, Weng Hua Khoo, Mate Biro, Tanmay Mitra, Michael Meyer-Hermann, Patrick Bertolino, Masato Tanaka, David A. Hume, Peter I. Croucher, Robert Brink, Akira Nguyen, Oliver Bannard, Tri Giang Phan
Summary: Germinal centers (GCs) in lymphoid follicles during antibody responses are areas of extensive cell death. Tingible body macrophages (TBMs) derived from lymph node-resident, CD169-lineage, CSF1R-blockade-resistant precursor. Non-migratory TBMs use cytoplasmic processes to capture migrating dead cell fragments using a lazysearch strategy. Follicular macrophages can mature into TBMs in the absence of GCs and activated by the nearby apoptotic cells. Single-cell transcriptomics analysis identified upregulated genes related to apoptotic cell clearance in TBMs, which prevent autoimmune diseases.
Article
Immunology
Anno Saris, Juulke Steuten, David P. Schrijver, Gijs van Schijndel, Jaap Jan Zwaginga, S. Marieke van Ham, Anja ten Brinke
Summary: Platelets have the ability to inhibit the pro-inflammatory properties of dendritic cells (DCs) and even induce an anti-inflammatory DC phenotype, with decreased T cell priming capacity by the DC. They do this through the secretion of soluble factors that inhibit DC activation and cytokine production, ultimately affecting T cell responses. These findings shed light on the potential role of platelets in immune modulation, particularly in the context of platelet transfusions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Szabolcs Muzsai, Ore-Matan Maryanovsky, Roland Ander, Gabor Koncz, Anett Mazlo, Attila Bacsi, Marta Toth
Summary: The cell-free supernatant (CFS) derived from Lactobacillus casei (L. casei), a member of human microbiota, enhances the sensitivity of mesenchymal-stromal-cell-like (MSCI) cells to viral stimuli and induces the development of dendritic cells (DCs) with anti-inflammatory and antiviral properties through pretreated MSCl cells. The presence of L. casei-derived extracellular vesicles in CFS during pretreatment is crucial for the production of INF & beta; and CXCL10 by MSCl cells upon viral stimulation. Moreover, L. casei CFS and/or poly (I:C)-conditioned MSCI cells alter the differentiation process and functional activities of freshly isolated monocytes and developing DCs.
Article
Immunology
Benjamin Hamid, Friederike Ebner, Lalita Bechtold, Arkadi Kundik, Sebastian Rausch, Susanne Hartmann
Summary: The study found that the relative frequencies of DC subsets in porcine blood differ, with A. suum E/S showing differential modulation on different DC subsets, suppressing pDC and cDC1 while upregulating cDC2.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jose L. Sanchez-Trincado, Hector F. Pelaez-Prestel, Esther M. Lafuente, Pedro A. Reche
Summary: The oral epithelial cells (OECs) have the ability to suppress immune responses by reducing the expression and release of specific molecules in dendritic cells (DCs) and impairing T cell activation. This suppression is mediated through the regulation of IFN gamma and TNF alpha production and is independent of specific interactions between CD40/CD40L, CD58/CD2, and PD-L1/PD-1.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Simone M. Hayen, Andre C. Knulst, Johan Garssen, Henny G. Otten, Linette E. M. Willemsen
Summary: This study investigated the effect of FF on DC maturation and allergen presentation, showing that FF can increase the secretion of IL-10 and IP-10 in mature DCs, promoting T cell polarization and proliferation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alberto N. Peon, Luis I. Terrazas
CURRENT MEDICINAL CHEMISTRY
(2016)
Article
Oncology
Gabriela Figueroa-Gonzalez, Veronica Garcia-Castillo, Jossimar Coronel-Hernandez, Eduardo Lopez-Urrutia, Sonia Leon-Cabrera, Luis E. Arias-Romero, L. I. Terrazas, Miriam Rodriguez-Sosa, Alma Delia Campos-Parra, Eduardo Zuniga-Calzada, Cesar Lopez-Camarillo, Fermin Morales-Gonzalez, Nadia J. Jacobo-Herrera, Carlos Perez-Plasencia
Article
Immunology
Marcela Alcantara-Hernandez, Rebecca Leylek, Lisa E. Wagar, Edgar G. Engleman, Tibor Keler, M. Peter Marinkovich, Mark M. Davis, Garry P. Nolan, Juliana Idoyaga
Article
Cell Biology
Arlett Espinoza-Jimenez, Roberto De Haro, Luis I. Terrazas
MEDIATORS OF INFLAMMATION
(2017)
Article
Cell Biology
Alberto N. Peon, Yadira Ledesma-Soto, Jonadab E. Olguin, Marcel Bautista-Donis, Edda Sciutto, Luis I. Terrazas
MEDIATORS OF INFLAMMATION
(2017)
Article
Oncology
Eduardo Lopez-Urrutia, Jossimar Coronel-Hernandez, Veronica Garcia-Castillo, Carlos Contreras-Romero, Antonio Martinez-Gutierrez, Diana Estrada-Galicia, Luis Ignacio Terrazas, Cesar Lopez-Camarillo, Hector Maldonado-Martinez, Nadia Jacobo-Herrera, Carlos Perez-Plasencia
Article
Oncology
Sonia A. Leon-Cabrera, Emmanuel Molina-Guzman, Yael G. Delgado-Ramirez, Armando Vazquez-Sandoval, Yadira Ledesma-Soto, Carlos G. Perez-Plasencia, Yolanda I. Chirino, Norma L. Delgado-Buenrostro, Miriam Rodriguez-Sosa, Felipe Vaca-Paniagua, Federico Avila-Moreno, Emma B. Gutierrez-Cirlos, Luis E. Arias-Romero, Luis I. Terrazas
CANCER IMMUNOLOGY RESEARCH
(2017)
Article
Oncology
Jonadab E. Olguin, Itzel Medina-Andrade, Emmanuel Molina, Armando Vazquez, Thalia Pacheco-Fernandez, Rafael Saavedra, Carlos Perez-Plasencia, Yolanda I. Chirino, Felipe Vaca-Paniagua, Luis E. Arias-Romero, Emma B. Gutierrez-Cirlos, Sonia A. Leon-Cabrera, Miriam Rodriguez-Sosa, Luis I. Terrazas
Article
Oncology
Felipe Galvez-Cancino, Ernesto Lopez, Evelyn Menares, Ximena Diaz, Camila Flores, Pablo Caceres, Sofia Hidalgo, Ornella Chovar, Marcela Alcantara-Hernandez, Vincenzo Borgna, Manuel Varas-Godoy, Flavio Salazar-Onfray, Juliana Idoyaga, Alvaro Lladser
Article
Multidisciplinary Sciences
C. E. Matisz, B. Faz-Lopez, E. Thomson, A. Al Rajabi, F. Lopes, L. I. Terrazas, A. Wang, K. A. Sharkey, D. M. McKay
SCIENTIFIC REPORTS
(2017)
Article
Neurosciences
Creed M. Stary, Lijun Xu, Ludmilla A. Voloboueva, Marcela Alcantara-Hernandez, Oiva J. Arvola, Juliana Idoyaga, Rona G. Giffard
FRONTIERS IN NEUROSCIENCE
(2019)
Article
Cell Biology
Rebecca Leylek, Marcela Alcantara-Hernandez, Zachary Lanzar, Anja Ludtke, Oriana A. Perez, Boris Reizis, Juliana Idoyaga
Article
Cell Biology
Rebecca Leylek, Marcela Alcantara-Hernandez, Jeffrey M. Granja, Michael Chavez, Kimberly Perez, Oscar R. Diaz, Rui Li, Ansuman T. Satpathy, Howard Y. Chang, Juliana Idoyaga
Article
Materials Science, Biomaterials
Gillie A. Roth, Olivia M. Saouaf, Anton A. A. Smith, Emily C. Gale, Marcela Alcantara Hernandez, Juliana Idoyaga, Eric A. Appel
Summary: The sustained release of vaccine cargo has shown to improve humoral immune responses to challenging pathogens like influenza. This study introduces an injectable polymer-nanoparticle hydrogel system that prolongs the co-delivery of antigen and adjuvant, resulting in increased potency and durability of influenza subunit vaccines.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biochemical Research Methods
Marcela Alcantara-Hernandez, Juliana Idoyaga
Summary: This study presents a detailed protocol for characterizing human dendritic cell populations using mass cytometry, including single-cell suspension preparation, staining, data analysis, etc., which can be completed in 2-3 days and paused conveniently. The emphasis is on enriching minor populations of immune cells using negative selection approaches.
