Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Aldo Meizoso-Huesca, Luke Pearce, Christopher J. Barclay, Bradley S. Launikonis
Summary: This study reveals the role of RyR1 Ca2+ leak in generating heat at SR Ca2+ pump in resting muscle. The RyR1 Ca2+ leak increases cytosolic [Ca2+] near SR Ca2+ pump, amplifying thermogenesis. The gene-dose-dependent increase in RyR1 leak leads to progressive rise in leak-dependent heat. Moreover, fibers from mice exhibit greater basal RyR Ca2+ leak and heat generated by SR Ca2+ pump compared to toads.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Crystal Seng, Luke Pearce, Aldo Meizoso-Huesca, Daniel P. Singh, Robyn M. Murphy, Cedric R. Lamboley, Bradley S. Launikonis
Summary: Ca2+ plays an important role in the functional and developmental regulation of mitochondria, regulating ATP resynthesis rate, the expression of PGC1a, and the generation of reactive oxygen species in skeletal muscle. Understanding the triggers for Ca2+ redistribution between intracellular compartments is crucial for determining mitochondrial Ca2+ load.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Andrej Pavlovic, Jana Jaksova, Zuzana Kucerova, Martina Spundova, Marek Rac, Pavel Roudnicky, Axel Mithofer
Summary: The general volatile anesthetic diethyl ether not only blocks sensation and responsive behavior in animals and plants, but also affects gene expression and downstream consequences in the plant Arabidopsis thaliana. The anesthesia causes reprogramming of gene expression, with thousands of genes being upregulated or downregulated. On the protein level, there are also significant changes in protein expression. The most impacted processes include chlorophyll/tetrapyrrole biosynthesis, photosynthesis, and the response to heat stress. The anesthesia also leads to increased cytoplasmic calcium level and cell membrane permeability for ions.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Pharmacology & Pharmacy
Yuxin Du, Laurie J. Demillard, Jun Ren
Summary: This article summarizes the normal regulation of SR Ca2+ in cardiomyocytes, the mechanisms of how Ca2+ triggers arrhythmias, the involvement of SR gene mutations in inherited arrhythmias, and the possible arrhythmogenic effects of these mutations.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Environmental Sciences
Jinshui Yang, Wenli Li, Chao Xing, Guanlan Xing, Yinxue Guo, Hongli Yuan
Summary: Microalgae, as the largest CO2 fixer and O2 producer, play an important role in human life and production. Heat stress has a significant impact on the growth and metabolism of microalgae. This study revealed that Ca2+ is involved in the regulation of lipid synthesis in microalgae under heat stress.
ENVIRONMENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Guizhen Fan, Mariah R. Baker, Lara E. Terry, Vikas Arige, Muyuan Chen, Alexander B. Seryshev, Matthew L. Baker, Steven J. Ludtke, David Yule, Irina I. Serysheva
Summary: In this study, the cryo-EM structures of IP(3)R1 were determined, providing atomic details of IP3, Ca2+ and ATP binding. The authors identified the molecular motions of key domains and sidechains that regulate ligand binding and gating behavior of IP3R channels.
NATURE COMMUNICATIONS
(2022)
Article
Biology
S. J. Richardson, C. G. Thekkedam, M. G. Casarotto, N. A. Beard, A. F. Dulhunty
Summary: Cardiac ryanodine receptors (RyR2) play a crucial role in releasing intracellular calcium essential for cardiac myocyte contraction. The regulation of ion channel opening by intracellular factors, including FK506 binding proteins FKBP12 and FKBP12.6, and their impact on RyR2 activity and cardiac contraction are still debated. This study demonstrates that FKBP12 activates RyR2 with high affinity and inhibits RyR2 with lower affinity, suggesting a model of negative cooperativity in FKBP12 binding. The dissociation of FKBP12/12.6 could dynamically affect RyR2 activity, indicating the significant role of FKBP12/12.6 in Ca2+ signaling and cardiac function.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Chris Lindsay, Maria Musgaard, Angela J. Russell, Rebecca Sitsapesan
Summary: This study found that all the statins examined could activate RyR1, and cerivastatin was the most potent in activating RyR1, supporting the hypothesis that RyR1 activation is implicated in statin-induced myopathy. Several statin analogues were identified that retained HMG-CoA reductase inhibition but lacked the ability to modulate RyR1.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Neurosciences
Binwen Liu, Xurui Zhu, Qingyun Zhou, Yan Su, Yue Qian, Zhengliang Ma, Xiaoping Gu, Tianjiao Xia
Summary: This study found that 6-hour isoflurane anesthesia induced cognitive dysfunction and increased RyR3 mRNA levels in the hippocampus. Caffeine, a RyR receptor agonist, corrected the disrupted expression of some cognitive-related proteins after anesthesia. Intraperitoneal injection of caffeine improved cognitive function after isoflurane anesthesia in mice.
BRAIN RESEARCH BULLETIN
(2023)
Article
Cell Biology
Daiki Watanabe, Masanobu Wada
Summary: The study found that the increased Ca2+ leakage after muscle contractions is mainly caused by the orthograde signal of DHPRs to RyRs. By experimentally measuring SR Ca2+ leakage in the skinned fibers under continuously depolarized conditions, it was shown that the level of Ca2+ leakage reached a similar level to that in stimulated fibers.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Michio Matsunobe, Norio Motohashi, Eito Aoki, Tsukasa Tominari, Masaki Inada, Yoshitsugu Aoki
Summary: Caveolin-3 plays a role in muscle cells and its overexpression is associated with muscle diseases. Suppression of Cav3 expression impairs myoblast differentiation and leads to increased intracellular Ca2+ and CaMKII levels. Inhibition of the Ryanodine receptor can rescue the impaired myoblast differentiation caused by Cav3 knockdown.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Agronomy
Wenjuan Mei, Yayun Zuo, Ting Su, Jing Yuan, Yidong Wu, Yihua Yang
Summary: A study identified a population of Spodoptera litura in Fujian province, China that showed resistance to diamide insecticides. Genetic analysis revealed that this resistance is associated with a ryanodine receptor mutation called I4728M. This finding is important for developing resistance monitoring tools and guiding resistance management practices.
PEST MANAGEMENT SCIENCE
(2023)
Article
Cardiac & Cardiovascular Systems
Chidinma A. Okolo, Ei-Phyo Khaing, Valeria Mereacre, Rachel S. Wallace, Michelle L. Munro, Jeffrey R. Erickson, Peter P. Jones
Summary: This study found that RyR2 can be directly regulated by O-GlcNAcylation and requires the presence of serine-2808. Under hyperglycemic conditions, O-GlcNAcylation is associated with an increase in Ca2+ leak through RyR2, and this increase persists after CaMKII inhibition.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Immunology
Jie Liao, Shaoshao Zhang, Shuaitao Yang, Yang Lu, Kai Lu, Yuwei Wu, Qiongfeng Wu, Ning Zhao, Qian Dong, Lei Chen, Yimei Du
Summary: IL-6 neutralization improved atrial inflammation and fibrosis, as well as atrial fibrillation susceptibility in ST rats, which is associated with Ca2+ handling abnormalities and RyR2 dysfunction.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Claudia Manini, Jose Lopez
Summary: Cancer is a complex ecosystem with intrinsic mechanisms that are not visible under the microscope. Pathologists provide necessary information for personalized treatments. This paper discusses the paradox of using static snapshots to make decisions for dynamic diseases, using clear cell renal cell carcinoma as an example. The aim is to raise awareness among pathologists and cancer-related medical specialists about the evolutionary features of the disease.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Biology
Annick Laruelle, Andre Rocha, Claudia Manini, Jose I. Lopez, Elena Inarra
Summary: In this study, interactions between cancer cells were explored using the hawk-dove game. The heterogeneity of tumors was analyzed by considering populations with 2 or 3 types of cells. The results showed that the best-off cell in the 2-type population game had a higher payoff than the best-off cell in the 3-type population game. The study highlights the importance of identifying intratumor heterogeneity in routine practice and suggests that therapeutic strategies preserving heterogeneity may slow down cancer growth.
BULLETIN OF MATHEMATICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jose L. Lopez, Joel L. Ramirez, Tuan Anh Phu, Phat Duong, Laura Bouchareychas, Christina R. Kuhrau, Pei-Yu Lin, Walter L. Eckalbar, Andrea J. Barczak, Joshua D. Rudolph, Lenka Maliskova, Michael S. Conte, Shant M. Vartanian, Robert L. Raffai, Adam Z. Oskowitz
Summary: This study aimed to identify novel biomarkers for abdominal aortic aneurysms (AAAs) by characterizing the expression profile of microRNAs (miRNA) in circulating exosomes. The study found that miR-122-5p levels were significantly reduced in the exosomes of AAA patients. This suggests that miR-122-5p could potentially be used as a diagnostic biomarker and have implications in AAA pathogenesis.
Article
Toxicology
Dong-Suk Kim, Isaac N. Pessah, Cristina M. Santana, Benton S. Purnell, Rui Li, Gordon F. Buchanan, Wilson K. Rumbeiha
Summary: Acute exposure to high concentrations of H2S can cause sudden death and neurological disorders. H2S exposure suppresses electrocerebral activity and disrupts breathing. Inhibition of certain Ca2+ channels and activation of TRP channels can prevent H2S-induced neurological damage.
TOXICOLOGICAL SCIENCES
(2023)
Correction
Multidisciplinary Sciences
Caroline E. Nunes-Xavier, Maite Emaldi, Janire Mingo, Tove Oyjord, Gunhild M. Maelandsmo, Oystein Fodstad, Peio Errarte, Gorka Larrinaga, Roberto Llarena, Jose I. Lopez, Rafael Pulido
SCIENTIFIC REPORTS
(2023)
Editorial Material
Oncology
Claudia Manini, Estibaliz Lopez-Fernandez, Jose I. Lopez, Javier C. Angulo
Article
Cardiac & Cardiovascular Systems
Fergie J. Losiniecki, Jose Lopez, Majd Jazaerly, Kristina Menchaca, Vivek Kothari, Brendon Cornett, Christopher N. Ochner, Robert Chait
Summary: This study aims to evaluate the predictive value of serum troponin I level on in-hospital mortality in COVID-19 patients during the first 24 hours of admission. The study found that a negative troponin I level during the first 24 hours of admission was associated with lower rates of in-hospital mortality and cardiac complications.
IJC HEART & VASCULATURE
(2023)
Article
Engineering, Biomedical
Massimo Salvi, Claudia Manini, Jose I. Lopez, Dario Fenoglio, Filippo Molinari
Summary: This study introduces a novel approach that combines diagnosis-specific immunohistochemical (IHC) staining and deep learning techniques to provide reliable stratification of prostate glands. The system achieved noteworthy results in specific-class gland quantification on whole slides, with a mean Dice Score Coefficient of 90.36% and a mean absolute error of 1.64%. The findings demonstrate the potential of this system as a valuable support tool for pathologists, reducing workload and decreasing diagnostic inter-observer variability.
COMPUTERIZED MEDICAL IMAGING AND GRAPHICS
(2023)
Article
Engineering, Biomedical
Jose Lopez, Justin Mark, Waseem Wahood, Nader Lamaa, Mauricio Danckers
Summary: Our study showed a significant decrease in in-hospital mortality rates among LVAD patients from 2017 to 2019, but no significant change in stroke rate trends. LVAD placement was associated with increased risks of stroke and in-hospital mortality.
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
(2023)
Article
Multidisciplinary Sciences
Leire Torices, Caroline Nunes-Xavier, Jose I. Lopez, Rafael Pulido
Summary: PTEN is a tumor suppressor gene frequently mutated in human tumors, and its mutations are the diagnostic marker for PTEN Hamartoma Tumor Syndrome (PHTS), a disorder characterized by multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. This study generated and characterized anti-PTEN monoclonal antibodies recognizing the PTEN C2-domain, which can be used to study the expression and function of PTEN isoforms and mutations associated with disease. These antibodies are suitable for investigating the pathogenicity of PTEN C-terminal truncations that have lost the PTEN C-terminal epitopes but retain stability and function.
Article
Oncology
Matthew R. Orton, Evan J. Hann, Simon J. Doran, Scott T. C. Shepherd, Derfel E. Ap Dafydd, Charlotte E. Spencer, Jose I. Lopez, Victor Albarran-Artahona, Francesca Comito, Hannah Warren, Joshua Shur, Christina Messiou, James Larkin, Samra Turajlic, TRACERx Renal Consortium, Dow-Mu Koh
Summary: The aim of this study is to evaluate the performance of radiomics predictions in ccRCC patients and demonstrate the impact of novel feature selection strategies and sub-segmentations on model interpretability. The proposed pipeline leads to more interpretable models without compromising prediction performance compared to the conventional pipeline.
Article
Geriatrics & Gerontology
Teresa Flores Ruano, Emiel O. Hoogendijk, Luis Romero Rizos, Gabriel Ariza Zafra, Matilde Leon Ortiz, Carmen Luengo Marquez, Elena Martin Senbastia, Jose Luis Navarro Lopez, Miguel Fernandez Sanchez, Rafael Garcia Molina, Almudena Avendano Cespedes, Victoria Sanchez-Flor Alfaro, Cristina Gomez Ballesteros, Rita Lopez Bru, Elsa Dent, Pedro Abizanda
Summary: This study examined the association between resting metabolic rate (RMR) and incident disability and mobility decline in older adults over a 10-year period. The results showed that higher RMR was associated with a lower risk of disability and mobility decline, especially in frail individuals.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2023)
Review
Communication
Jose Luis Portela Lopez, Carlos Rodriguez Monroy
Summary: The advancements in technology have led to innovations in the organizational field, specifically in terms of product and service innovation. These innovations focus on understanding the real needs of consumers and the impact of their mental processes on their behavior. The integration of neuroeconomics and neuroscience provides a novel approach to analyze consumer behavior and the decision-making process.
REVISTA LATINA DE COMUNICACION SOCIAL
(2023)
Article
Medicine, General & Internal
Claudia Manini, Claudia Provenza, Leire Andres, Igone Imaz, Rosa Guarch, Raffaelle Nunziata, Jose I. Lopez
Summary: Tumor-to-tumor metastasis is a rare event that requires accurate identification by pathologists. It can be straightforward to identify when the tumors have different histologies, but can be challenging when two carcinomas with similar cytoarchitecture are present, one inside the other under the microscope. In this study, we report four cases involving clear cell renal cell carcinoma and highlight the difficulties in recognizing some of them. An appropriate clinical-pathological correlation, including a review of the patient's history and radiological exams, would greatly aid in routine identification of tumor-to-tumor metastases.
CLINICS AND PRACTICE
(2023)
Article
Medicine, General & Internal
Claudia Manini, Estibaliz Lopez-Fernandez, Nicola Cruciano, Alessandro Comandone, Jose I. Lopez
Summary: The correct diagnosis of mesothelial proliferations is a classic challenge for pathologists, and it holds significant clinical implications. These proliferations are frequently associated with recurrent hydrocele, and their morphological characteristics can resemble malignancy, leading to potential misdiagnosis.
CLINICS AND PRACTICE
(2023)