期刊
FASEB JOURNAL
卷 24, 期 6, 页码 2116-2125出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.09-146167
关键词
inflammation; polymorphonuclear leukocytes; PARs; primary granules
资金
- Canadian Institutes of Health Research (CIHR) [MOP-64315]
- U.S. National Institutes of Health [R01 AR052614]
- Fonds de la Recherche en Sante du Quebec (FRSQ)
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR052614] Funding Source: NIH RePORTER
We shed new light on the expression and function of the proteinase-activated receptor (PAR) family, associated with inflammation and hyperalgesia, in human granulocytes. Resting cells expressed constitutive levels of PAR-2 and PAR-3 mRNA but not PAR-1 or PAR-4. Based on flow cytometry, stimulation with opsonized bacteria (Bop) specifically up-regulated cell surface expression of PAR-2 in a concentration-dependent and time-dependent manner, independent of transcription or de novo protein synthesis. Primary granules were identified as a source of preformed PAR-2 that can readily be mobilized at the surface on fusion with the plasma membrane. Cellular response to PAR-2 activation, measured as changes in intracellular calcium concentration, was enhanced in PAR-2 up-regulated cells. Increase of cell-surface PAR-2 and of cell responsiveness were dependent specifically on the engagement of immunoglobulin (Ig)-binding receptors. Together, our results reveal that mobilization of intracellular granules, in response to Ig-receptor activation, up-regulates PAR-2 surface expression and makes neutrophils more responsive to proteinase activity. This enhanced response to PAR-2 activation indicates that molecular communication between pain and inflammation may be more important than previously believed.-St-Onge, M., Lagarde, S., Laflamme, C., Rollet-Labelle, E., Marois, L., Naccache, P. H., Pouliot, M. Proteinase-activated receptor-2 upregulation by Fc gamma-receptor activation in human neutrophils. FASEB J. 24, 2116-2125 (2010). www.fasebj.org
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