Article
Chemistry, Applied
Shinichiro Suto, Ikuko Kakizaki, Yota Tatara, Masahiko Endo
Summary: The structure of hyaluronan is crucial for binding to HABP, with particular emphasis on the non-reducing terminal structure. Replacement of internal disaccharide units is possible for chains decasaccharide or larger.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Shuko Terazawa, Mariko Takada, Yoriko Sato, Hiroaki Nakajima, Genji Imokawa
Summary: This study found that UVA radiation decreases the secretion of HA by HDFs due to the regulation of HAS2 and HYBID expression levels, mainly attributed to the inactivated signaling of the STAT3 axis and the activated signaling of the p38/ATF2 axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Giulia Giubertoni, Alberto Perez de Alba Ortiz, Fouzia Bano, Xing Zhang, Robert J. Linhardt, Dixy E. Green, Paul L. DeAngelis, Gijsje H. Koenderink, Ralf P. Richter, Bernd Ensing, Huib J. Bakker
Summary: The study reveals that calcium ions specifically bind to hyaluronan, weakening its intramolecular hydrogen bond network and increasing the flexibility of the polymer chain. Furthermore, the binding of calcium saturates at a certain fraction, reducing the probability of subsequent binding at neighboring sites.
Article
Biochemistry & Molecular Biology
Sheiny Tjia-Fleck, Bradley M. Readnour, Yetunde A. Ayinuola, Francis J. Castellino
Summary: Cellular plasminogen receptors are important for recruiting and activating plasmin, and protecting it from inactivation. The enolase enzyme in Streptococcus pyogenes can function as a plasminogen receptor, and its structure and binding sites were investigated.
Article
Multidisciplinary Sciences
Li-Jin Chan, Anugraha Gandhirajan, Lenore L. Carias, Melanie H. Dietrich, Oscar Vadas, Remy Visentin, Camila T. Franca, Sebastien Menant, Dominique Soldati-Favre, Ivo Mueller, Christopher L. King, Wai-Hong Tham
Summary: The study shows that antibodies against Plasmodium vivax RBP2b can provide protection against infection and disease, and inhibit invasion of reticulocytes. Researchers have identified different mechanisms by which human antibodies can inhibit the invasion ability of P. vivax RBP2b.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xing Fan, Lipeng Xu, Jianhao Zhang, Yidan Wang, Zirui Wu, Wenjing Sun, Xin Yao, Xu Wang, Shanshan Guan, Yaming Shan
Summary: This study explores the potential of single-chain variable fragments (scFvs) as immunotherapeutic agents for Alzheimer's disease (AD). By designing and constructing scFvs targeting amyloid-beta-42 protein (A beta 42), namely scFv-C, scFv-S, and scFv-12B4, the researchers evaluated their thermal stabilities and binding ability to A beta 42. They found that scFv-C could recognize A beta 42 monomer/oligomer and promote disaggregation of A beta 42 fiber. Molecular dynamics analysis revealed the interactions between scFv-C and A beta 42, suggesting the possibility of inhibiting further aggregation. These scFvs, particularly scFv-C, show promising biological activity and potential for clinical application in AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura-Someya, Mikako Shirouzu, Masayoshi Fukasawa
Summary: The study demonstrates that the conversion of monoclonal antibodies to monovalent antigen-binding fragments and single-chain variable fragment antibodies maintains high binding specificity and affinity, and also shows inhibition of HCV infection. These functional monovalent OCLN-binding probes have potential as drug candidates and tools for studies of OCLN in biology and pharmaceuticals.
Article
Polymer Science
Lu Qian, Kai Zhang, Xin Guo, Junyu Zhou, Miao Yu
Summary: The study found that there was almost no difference in the single-chain elasticity of gelatin in nonane and DI water. When a single gelatin chain is pulled into loose nonane, dehydration does not occur due to strong binding water interactions. Gelatin chains can only interact with water molecules at high temperatures.
Letter
Medical Laboratory Technology
Batia Kaplan, Joannes F. M. Jacobs
Summary: The study found that the interaction between Freelite and FLC dimers is better than with FLC monomers, while N Latex FLC mainly recognizes FLC monomers and has poor reactivity with FLC dimers. This difference may explain discrepancies in FLC quantification between Freelite and other immunochemical assays like N-Latex FLC.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Ana Freitas, Lucilia Domingues, Tatiana Q. Aguiar
Summary: This review highlights the recent advances in tag-mediated protein purification and immobilization, emphasizing their importance in developing efficient single-step purification and immobilization strategies. The review also summarizes the latest progress in creating protein-engineered materials using innovative tag combinations and identifies future opportunities.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Materials Science, Biomaterials
Francisco J. Reche-Perez, Simona Plesselova, Eduardo De los Reyes-Berbel, Mariano Ortega-Munoz, Francisco J. Lopez-Jaramillo, Fernando Hernandez-Mateo, Francisco Santoyo-Gonzalez, Rafael Salto-Gonzalez, Maria D. Giron-Gonzalez
Summary: The use of monoclonal antibody fragments like ScFv for targeted delivery of antitumor therapeutics to cancer cells is promising. A non-covalent approach using MBP-ScFv fusion protein as a binding adapter allows for flexible interchange of cargos and ScFv coding sequences, providing targeted delivery solutions without additional optimization. The modularity of the platform makes it versatile for various applications in diagnosis, imaging, and drug delivery to tumors.
BIOMATERIALS SCIENCE
(2021)
Article
Chemistry, Analytical
Thuy Tran, Erik Martinsson, Sergio Vargas, Ingemar Lundstrom, Carl-Fredrik Mandenius, Daniel Aili
Summary: The production of therapeutic monoclonal antibodies is a complex process that requires extensive analytical characterization. Aggregation of antibodies is common and problematic, as it can affect the product quality and immunogenicity. Rapid detection of aggregates is challenging with current analytical techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Arthur J. Schick, Li Yi, Philippe Lam, Preston Pallante, Nathaniel Swanson, Jacqueline Y. Tyler
Summary: Sterile filtration is a critical step in the manufacturing process of biological therapeutics, but protein adsorption to filter surfaces can lead to issues. By adjusting the Debye screening length parameters of the solution, the loss of high molecular weight species during filtration can be influenced.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yang Xu, Fanen Yuan, Qian Sun, Linyao Zhao, Yu Hong, Shiao Tong, Yangzhi Qi, Liguo Ye, Ping Hu, Zhang Ye, Si Zhang, Baohui Liu, Qianxue Chen
Summary: RNA-binding protein CSTF2 is upregulated in human glioblastoma (GBM) and promotes GBM cell survival by binding to the mRNA of the BAD protein to shorten its 3' UTR, resulting in the destabilization of BAD mRNA and inhibition of BAD-mediated apoptosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Jiahao Xia, Jiacheng Zuo, Hongbin Li
Summary: In this study using single molecule force spectroscopy, it was found that the small globular protein GB1 exhibits molecular homogeneity in its mechanical unfolding reaction, with ensemble averages and time averages of physical quantities showing no differences among individual molecules. This suggests that the ergodic hypothesis holds true for GB1 on the time scale of the force spectroscopy experiments.