期刊
FASEB JOURNAL
卷 22, 期 5, 页码 1540-1551出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.07-9694com
关键词
transient receptor potential; TRPM2; TRPM7; TRPM8; nudix
The mammalian TRPM gene family can be subdivided into distinct categories of cation channels that are either highly permeable for Ca(2+) (TRPM3/6/7), nonselective (TRPM2/8), or even Ca(2+) impermeable (TRPM4/5). TRPM6/7 are fused to alpha-kinase domains, whereas TRPM2 is linked to an ADPribose phosphohydrolase (Nudix domain). At a molecular level, the evolutionary steps that gave rise to the structural and functional TRPM channel diversity remain elusive. Here, we provide phylogenetic evidence that Nudix-linked channels represent an ancestral type of TRPMs that is present in various phyla, ranging from protists to humans. Surprisingly, the pore-forming segments of invertebrate TRPM2-like proteins display high sequence similarity to those of Ca(2+)-selective TRPMs, while human TPPM2 is characterized by a loss of several conserved residues. Using the patch-clamp technique, Ca(2+) imaging, and site-directed mutagenesis, we demonstrate that restoration of only two ancient pore residues in human TRPM2 (Q981E/P983Y) significantly increased (similar to 4-fold) its permeability for Ca(2+) Conversely, introduction of a modern sequence motif into mouse TRPM7 (EI047Q/YI049P) resulted in the loss of Ca(2+) permeation and a linear TRPM2-like current-voltage relationship. Overall, our findings provide an integrative view on the evolution of the domain architecture and the structural basis of the distinct ion permeation profiles of TRPM channels.
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