Article
Engineering, Environmental
Siti Shilatul Najwa Sharuddin, Siti Rozaimah Sheikh Abdullah, Hassimi Abu Hasan, Ahmad Razi Othman, Nur 'Izzati Ismail
Summary: This study isolated multifunctional rhizobacteria from different sources, such as rhizosphere soils and crude oil sludge, and found that these bacteria can efficiently degrade hydrocarbons while producing biosurfactants. The presence of biosurfactants was confirmed by SEM images, showing the formation of exopolymers that enhance the degradation process.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Multidisciplinary Sciences
Phillip Nussbaum, Maren Gerstner, Marie Dingethal, Celine Erb, Sonja-Verena Albers
Summary: Cell division in most bacteria and many archaea relies on the proteins FtsZ and SepF, but a homolog of SepF in archaea, such as Haloferax volcanii, also plays a crucial role in cell division by interacting with FtsZ1 and FtsZ2. The archaeal SepF lacks a conserved glycine residue important for polymerization in bacteria, suggesting a different mechanism of action in archaea.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Norio Saito, Sekito Itoyama, Rieko Takahashi, Yutaka Takahashi, Yukishige Kondo
Summary: This study investigated hybrid surfactants containing alkyl and fluoroalkyl chains, finding them to be superhydrophobic and capable of switching surface activity with an external light source. Experiments showed that compositions with octyl-fluorocarbon chains exhibited higher surface tension variation, indicating promising applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Microbiology
Yan Liao, Verena Vogel, Sabine Hauber, Juergen Bartel, Omer S. Alkhnbashi, Sandra Maass, Thandi S. Schwarz, Rolf Backofen, Doerte Becher, Iain G. Duggin, Anita Marchfelder
Summary: The transcription factor CdrS plays a central role in coordinating metabolism and cell division in the archaeon Haloferax volcanii. It regulates the expression of genes associated with cell division, protein degradation, and metabolism. Additionally, CdrS has multiple DNA-binding sites and is involved in the regulation of essential cell division genes, suggesting its sophisticated role in coordinating cellular pathways.
Article
Biotechnology & Applied Microbiology
Manuel Alejandro Lizardi-Jimenez, Ricardo Hernandez-Martinez
Summary: Our study demonstrates that considering the mass transfer of oil is more important than that of oxygen in multiphase systems. The transfer coefficient of oxygen is crucial in the design of aerobic bioreactors, but the oil transfer is often overlooked in systems with non-soluble substrates. Emulsification and surface tension can significantly affect the size of oil droplets and interfacial transfer, playing an important role in the degradation of non-soluble substrates.
Article
Microbiology
Megha Patro, Iain G. Duggin, Sonja-Verena Albers, Solenne Ithurbide
Summary: This study investigates the impact of auxotrophic mutations and the presence of plasmids on the cell shape of Haloferax volcanii. The results show that most auxotrophic strains have variations in cell shape parameters and the presence of plasmids also impacts these parameters. The Delta hdrB strains and hdrB selection markers have the most influence on H. volcanii cell shape.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Theopi Rados, Katherine Andre, Micaela Cerletti, Alex Bisson
Summary: This study developed a new xylose-inducible promoter, Pxyl, and characterized its function using msfGFP as a reporter gene. The results showed that the Pxyl promoter exhibited higher levels of gene expression in the presence of xylose compared to the previously described inducible promoter, PtnaA. Additionally, the study validated the inducibility of Pxyl by reproducing morphological phenotypes through the overexpression of FtsZ1 and FtsZ2 genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Daniel Wasser, Andreas Borst, Mathias Hammelmann, Katharina Ludt, Jorg Soppa
Summary: Research has shown that unselected intermolecular gene conversion is highly efficient in archaea, with longer conversion tracts compared to bacteria. Gene conversion frequency is independent of genome differences, and even a single nucleotide difference can trigger conversion.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Ambika Dattani, Itai Sharon, Ella Shtifman-Segal, Shachar Robinzon, Uri Gophna, Thorsten Allers, Neta Altman-Price
Summary: Polyploidy is common in haloarchaea, but it is generally considered as an evolutionary trap that leads to the decline or even extinction of a species. In most reported cases, polyploid organisms have an identical genetic state. However, our study found that Haloferax volcanii, a halophilic archaeon, maintains homoploidy, while its close relative H. mediterranei maintains heteroploidy and shows a low rate of homologous recombination. We suggest that H. volcanii escapes Muller's ratchet through an effective chromosome-equalizing gene-conversion mechanism facilitated by highly active homologous recombination, while H. mediterranei must have a different mechanism to elude the ratchet.
G3-GENES GENOMES GENETICS
(2023)
Article
Cell Biology
Andriko von Kugelgen, Vikram Alva, Tanmay A. M. Bharat
Summary: The S-layer is an ordered proteinaceous structure covering prokaryotic cells, with SLPs being the most abundant macromolecules in prokaryotes and playing critical roles in cellular physiology. Research reveals that the S-layer is mainly composed of a hexagonal array of tightly interacting immunoglobulin-like domains, while cellular tomography shows the nearly continuous presence of the S-layer on the cell surface.
Article
Genetics & Heredity
Friedhelm Pfeiffer, Mike Dyall-Smith
Summary: Annotation ambiguities and errors are common challenges in genomics. This study identified incorrectly annotated proteins in Haloferax volcanii and proposed alternative, possibly correct, functions as well as likely invalid functions. By compiling and summarizing these issues, the study aims to improve the evaluation of protein function assignments and provide a framework for experimental validation or discovery of unexpected functions in the scientific community.
Review
Microbiology
Rosana E. De Castro, Maria I. Gimenez, Micaela Cerletti, Roberto A. Paggi, Mariana I. Costa
Summary: This article discusses the role of archaeal membrane proteases in cell physiology and their importance in adapting to hostile environments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Polymer Science
Enrique Sanchez-Leon, Elisa Huang-Lin, Ricardo Amils, Concepcion Abrusci
Summary: The Bacillus amyloliquefaciens RT7 strain was isolated from an extreme acidic environment and evaluated for its biodegradation capabilities using different carbon sources. The exopolysaccharide (EPSRT7) produced during the degradation of glucose-Tween 80 was isolated and characterized. EPSRT7 exhibited good emulsifying activity and antioxidant capacity, making it promising for bioremediation processes and other industrial applications.
Article
Chemistry, Applied
Shunji Sakai, Nobuaki Ikeda
Summary: A new method was proposed to accurately predict the emulsifying activity of egg yolk by measuring and analyzing the interfacial tension between egg yolk and soybean oil under different treatment conditions. The method can identify the most optimum pasteurization condition for egg yolk at an industrial scale by calculating the correlation between different physical properties and the emulsifying activity.
FOOD HYDROCOLLOIDS
(2022)
Article
Genetics & Heredity
Alexei Cooper, Andrea M. Makkay, R. Thane Papke
Summary: Deletion mutants of all eight tubulin-like genes in Haloferax volcanii significantly impact cell morphology, suggesting that these genes indeed have a function.
Review
Biochemistry & Molecular Biology
Yannick Y. Corsellis, Marc M. Krasovec, Lea L. Sylvi, Philippe P. Cuny, Cecile C. Militon
Article
Ecology
F. Gilbert, S. Hulth, V. Grossi, R. C. Aller
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2016)
Article
Genetics & Heredity
Cecile Militon, Alexandre Atkinson, Valerie Michotey, Celine Jeziorski, Cristiana Cravo-Laureau, Robert Duran, Patricia Bonin, Philippe Cuny
Article
Geochemistry & Geophysics
Arnauld Vincon-Laugier, Vincent Grossi, Muriel Pacton, Gilles Escarguel, Cristiana Cravo-Laureau
ORGANIC GEOCHEMISTRY
(2016)
Article
Geography, Physical
J. Plancq, E. Mattioli, B. Pittet, F. Baudin, L. V. Duarte, M. Boussaha, V. Grossi
PALAEOGEOGRAPHY PALAEOCLIMATOLOGY PALAEOECOLOGY
(2016)
Article
Microbiology
Arnauld Vincon-Laugier, Cristiana Cravo-Laureau, Isabelle Mitteau, Vincent Grossi
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Environmental Sciences
J. Sturla Lompre, M. Nievas, M. Franco, V Grossi, A. Ferrando, C. Militon, F. Gilbert, P. Cuny, G. Stora, M. Sepulveda, J. Esteves, M. Commendatore
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2018)
Article
Geosciences, Multidisciplinary
Matthew Makou, Timothy Eglinton, Cameron McIntyre, Daniel Montlucon, Ingrid Antheaume, Vincent Grossi
GEOPHYSICAL RESEARCH LETTERS
(2018)
Article
Geochemistry & Geophysics
Arnauld Vincon-Laugier, Cristiana Cravo-Laureau, Vincent Grossi
ORGANIC GEOCHEMISTRY
(2018)
Article
Geology
Camille Thomas, Vincent Grossi, Ingrid Antheaume, Daniel Ariztegui
Article
Ecology
C. Bernard, A. Escalas, N. Villeriot, H. Agogue, M. Hugoni, C. Duval, C. Carre, P. Got, G. Sarazin, D. Jezequel, C. Leboulanger, V Grossi, M. Ader, M. Troussellier
Article
Forestry
Claire Michelet, Daniela Zeppilli, Cedric Hubas, Elisa Baldrighi, Philippe Cuny, Guillaume Dirberg, Cecile Militon, Romain Walcker, Dominique Lamy, Ronan Jezequel, Justine Receveur, Franck Gilbert, Amonda El Houssainy, Aurelie Dufour, Lars-Eric Heimburger-Boavida, Isabelle Bihannic, Lea Sylvi, Baptiste Vivier, Emma Michaud
Summary: Bioindicators assess the mangroves ecological state according to the pressures, with benthic meiofauna diversity and structure responding to sediment variables and contaminant concentrations. Different meiofauna species showed varying responses to the environmental conditions, highlighting their sensitivity to ecological changes. Further research on different scales and levels of deterioration is needed to utilize meiofauna as reliable bioindicators for mangrove ecosystems.
Article
Environmental Sciences
Maud Fiard, Philippe Cuny, Lea Sylvi, Cedric Hubas, Ronan Jezequel, Dominique Lamy, Romain Walcker, Amonda El Houssainy, Lars-Eric Heimburger-Boavida, Tony Robinet, Isabelle Bihannic, Franck Gilbert, Emma Michaud, Guillaume Dirberg, Cecile Militon
Summary: The study characterized changes in the structure and diversity of microbial communities of mangroves along the urban-to-rural gradient of the Cayenne estuary, which experience low human impact. Environmental determinants like granulometry, dieldrin concentration, pH, and total carbon content were found to significantly correlate to the microbial communities in these mangroves. Different types of weak anthropogenic pressure, such as organic matter enrichment, dieldrin contamination, and naphthalene contamination, were identified to impact the microbial populations and assemblages in the mangroves.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Ecology
Agustina Ferrando, Julieta Sturla Lompre, Emilia Gonzalez, Marcos Franco, Marta Commendatore, Marina Nievas, Cecile Militon, Georges Stora, Jose Luis Esteves, Philippe Cuny, Franck Gilbert
Summary: This study evaluated the seasonal variation of the macrobenthic communities in Caleta Valdes (CV) and validated an ex situ experimental protocol. The study found that polychaete and crustacean species dominated the macrobenthic community, with higher abundances in spring and summer. Both in situ and ex situ measurements yielded similar results, confirming the effectiveness of the ex situ experimental procedure. These findings provide important baseline information for monitoring potential pollution effects in Patagonian coastal systems.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)