期刊
EXTREMOPHILES
卷 17, 期 2, 页码 265-275出版社
SPRINGER JAPAN KK
DOI: 10.1007/s00792-013-0514-z
关键词
Haloarchaea; Cell sorting; Genome amplification; Biogeography; Nanohaloarchaea; Prokaryotic speciation
资金
- National Science Foundation [0919290, 080024]
- U.S.-Israel Binational Science Foundation [2007043]
- NASA Astrobiology: Exobiology and Evolutionary Biology Program [NNX12AD70G]
- Direct For Biological Sciences
- Division Of Environmental Biology [919290, 0830024, 0841933] Funding Source: National Science Foundation
Biogeography of microbial populations remains to be poorly understood, and a novel technique of single cell sorting promises a new level of resolution for microbial diversity studies. Using single cell sorting, we compared saturated NaCl brine environments (32-35 %) of the South Bay Salt Works in Chula Vista in California (USA) and Santa Pola saltern near Alicante (Spain). Although some overlap in community composition was detected, both samples were significantly different and included previously undiscovered 16S rRNA sequences. The community from Chula Vista saltern had a large bacterial fraction, which consisted of diverse Bacteroidetes and Proteobacteria. In contrast, Archaea dominated Santa Pola's community and its bacterial fraction consisted of the previously known Salinibacter lineages. The recently reported group of halophilic Archaea, Nanohaloarchaea, was detected at both sites. We demonstrate that cell sorting is a useful technique for analysis of halophilic microbial communities, and is capable of identifying yet unknown or divergent lineages. Furthermore, we argue that observed differences in community composition reflect restricted dispersal between sites, a likely mechanism for diversification of halophilic microorganisms.
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