期刊
EXTREMOPHILES
卷 18, 期 1, 页码 99-110出版社
SPRINGER JAPAN KK
DOI: 10.1007/s00792-013-0601-1
关键词
Archaeal; Ferredoxin; Ferredoxin: NADP(+) oxidoreductase; Thioredoxin reductase; Sulfolobus
资金
- Japan Society for the Promotion of Science [120600000293]
- Grants-in-Aid for Scientific Research [24580136] Funding Source: KAKEN
The putative gene (st2133) for ferredoxin:NADP(+) oxidoreductase (FNR) from Sulfolobus tokodaii, a thermoacidophilic crenarchaeon, was heterologously expressed. About 90 % of the purified product was a homodimer containing 0.46 mol FAD/mol subunit, and showing NADPH:DCPIP oxidoreductase activity, V (max) being 1.38 and 21.8 U/mg (70 A degrees C) in the absence and presence of 1 mM FMN. NADPH was a much better electron donor than NADH with various electron acceptors, such as oxygen, hydrogen peroxide, DCPIP, cytochrome c, and dithiobisnitrobenzoate. Most of the reactions were activated by 15- to 140-fold on addition of FMN, while FAD was 5-10 times less effective. Ferredoxin (Fd) from S. tokodaii served as an electron carrier in both Fd-dependent NADPH formation and NADPH-dependent Fd reduction. ST2133 belongs to the thioredoxin reductase-like protein family, which is slightly distantly related to FNR family proteins from bacteria, plants and man. This is the first report on FNR from a crenarchaeon, providing a clue to the recycling of Fd during archaeal metabolism.
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