期刊
EXPRESS POLYMER LETTERS
卷 8, 期 8, 页码 565-573出版社
BUDAPEST UNIV TECHNOL & ECON
DOI: 10.3144/expresspolymlett.2014.60
关键词
polymer membranes; electrospun; glucose biosensor; immobilization; graphene
资金
- National Science Council of Taiwan, ROC [NSC 101-2221-E-035-013, NSC 102-2221-E-011 -160]
In this study, poly(vinyl alcohol) (PVA)/Glucose oxidase (GOx)/graphene biocomposite membranes were prepared using an electrospinning technique and used for enzyme immobilization. The PVA/GOx/graphene membrane's morphology was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while its electrochemical sensitivity was studied by chronoamperometry. Kinetic parameters were determined to clarify the role of graphene in enzyme immobilization, while a spectrophotometric assay was used to quantify the active enzyme. The results indicated that the presence of graphene helps to stabilize the enzyme's conformation, facilitate the catalytic reaction, and increase the survivability of the enzyme.
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