Article
Microbiology
Ruikang Liu, Jeffrey L. Americo, Patricia L. Earl, Jack Villani, Catherine A. Cotter, Bernard Moss
Summary: The difference in pathogenicity between the pathogenic isolate clone 3 (CL3) and the second-generation smallpox vaccine ACAM2000 is associated with truncation of the interferon alpha/beta (IFN-alpha/beta) decoy receptor. Viruses expressing the full-length decoy receptor are more virulent in mouse models.
Article
Microbiology
Ruikang Liu, Jeffrey L. Americo, Patricia L. Earl, Jack Villani, Catherine A. Cotter, Bernard Moss
Summary: The more pathogenic Clone 3 (CL3) virus compared to ACAM2000 is attributed to the presence of a full-length IFN-alpha/beta decoy receptor in CL3 and a truncation of the receptor in ACAM2000. Experimental evidence shows that viruses expressing the full-length decoy receptor cause more severe diseases in mice.
Review
Biochemistry & Molecular Biology
Beatriz Perdiguero, Patricia Perez, Laura Marcos-Villar, Guillermo Albericio, David Astorgano, Enrique Alvarez, Laura Sin, Carmen Elena Gomez, Juan Garcia-Arriaza, Mariano Esteban
Summary: This review explores the history and biology of poxviruses, focusing on their application as vaccines against diseases such as smallpox, monkeypox, and other viral diseases. It discusses the current challenges and future prospects for improving the immunogenicity and efficacy of poxvirus-based vaccines.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Immunology
Huy Quang Quach, Krista M. Goergen, Diane E. Grill, Iana H. Haralambieva, Inna G. Ovsyannikova, Gregory A. Poland, Richard B. Kennedy
Summary: This study identified shared gene expression pathways in response to influenza and vaccinia virus stimulation in different immune cells. The immune response signaling pathways identified can be targeted for the development of broad therapeutics and vaccines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Sushmita Kumari, Jayeshbhai Chaudhari, Qinfeng Huang, Phillip Gauger, Marcelo Nunes De Almeida, Yuying Liang, Hinh Ly, Hiep L. X. Vu
Summary: This study evaluated the feasibility of using rPICV as a viral vector to deliver HA antigen and protect pigs against IAV-S challenge. The results demonstrated that the rPICV-H3 vectored vaccine elicited protective immunity against IAV-S.
Article
Immunology
Tetsuya Saito, Yukiya Sako, Fumi Sato-Kaneko, Tadashi Hosoya, Shiyin Yao, Fitzgerald S. Lao, Jonathan Shpigelman, Karen Messer, Minya Pu, Nikunj M. Shukla, Michael Chan, Paul J. Chu, Howard B. Cottam, Tomoko Hayashi, Dennis A. Carson, Maripat Corr
Summary: A potential adjuvant molecule (2D216) was found to enhance TLR4 agonist-mediated immune responses in vitro. In murine vaccination studies, the combination of 2D216 with MPLA acted as a potent co-adjuvant, increasing antigen-specific IgG levels and protecting against lethal influenza virus challenge. The co-adjuvant MPLA/2D216 produced Th1 dominant immune responses and showed excellent safety with minimal local reactogenicity and no systemic inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Nadia Vaez G. Cruz, Matheus Nobrega Luques, Terezinha Marta Pereira P. Castineiras, Jr Orlando Costa Ferreira, Regina Helena S. Peralta, Luciana J. da Costa, Clarissa R. Damaso
Summary: Researchers analyzed the complete genome sequences of three VACV-Wyeth clonal isolates obtained from Brazil in the 1970s, finding high similarity with Dryvax clones. Recombination events were detected between Wyeth A111 and Acam2000 clones, suggesting similar patterns in other regions of the genomes. A small-scale serological survey revealed the presence of anti-VACV IgG antibodies in individuals born before the end of smallpox vaccination in Brazil, demonstrating the usefulness of the VACV-Wyeth strain in future serological studies.
Article
Engineering, Biomedical
Yufeng Song, Wandi Zhu, Ye Wang, Lei Deng, Yao Ma, Chunhong Dong, Gilbert X. Gonzalez, Joo Kim, Lai Wei, Sang -Moo Kang, Bao-Zhong Wang
Summary: Influenza B vaccine efficacy is limited by antigenicity changes, but this study developed double-layered protein nanoparticles incorporating structure-stabilized antigens, effectively inducing broad immune responses and providing cross-immune protection against multiple influenza B virus strains.
Article
Biochemistry & Molecular Biology
Suyeon Kim, Aleksandra Nowakowska, Young Bong Kim, Ha Youn Shin
Summary: This study established cell lines deficient in both type I and type II interferon responses using CRISPR-Cas9 system, which promoted virus replication and will be useful in viral studies and the development of novel vaccines and therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Shelby Landreth, Susan Detmer, Volker Gerdts, Yan Zhou
Summary: The study designed a bivalent live attenuated influenza virus vaccine using Canadian swIAV isolates, which showed strong potential to protect swine against the predominant influenza subtypes in North America.
VETERINARY MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Lynda Coughlan, Eric J. Kremer, Dmitry M. Shayakhmetov
Summary: Zoonotic viruses pose a continuous threat, and investing in vaccine development is crucial. Replication-defective adenoviral vectors have shown potential as vaccine platforms. This review discusses the progress in using Ad-based vaccines against emerging viruses and summarizes their global safety profile during the SARS-CoV-2 pandemic.
Review
Immunology
Claudia Maria Trombetta, Otfried Kistner, Emanuele Montomoli, Simonetta Viviani, Serena Marchi
Summary: Influenza is a vaccine preventable disease and vaccination is the most effective method of controlling the morbidity and mortality of seasonal influenza, especially among risk groups. The effectiveness of current influenza vaccines is suboptimal, but they are still effective against morbidity and mortality in all age and risk groups, particularly in young children and older adults.
Article
Immunology
Narendar Kumar, Fatima Ahmed, Muhammad Sauban Raza, Pushp Lata Rajpoot, Wajiha Rehman, Shoaib Alam Khatri, Mustapha Mohammed, Shaib Muhammad, Rabbiya Ahmad
Summary: This study aimed to explore knowledge, attitude, perceptions, and willingness regarding vaccination among university students in Pakistan. The majority of the respondents had average knowledge and neutral attitudes towards monkeypox. The study revealed a significant association between knowledge of monkeypox and the type of academic degree, study discipline, and region of respondents. The findings emphasize the need to raise public awareness and educate students on monkeypox virus to improve adherence to preventative recommendations.
Article
Biochemistry & Molecular Biology
Wahiba Ezzemani, Ahd Ouladlahsen, Haya Altawalah, Rachid Saile, M'hammed Sarih, Anass Kettani, Sayeh Ezzikouri
Summary: The researchers used in silico structural biology and advanced immunoinformatics to design a multi-epitope subunit vaccine against monkeypox virus (MPV) infection. The modified vaccine showed strong interaction with Toll-like receptor 9 (TLR9) and demonstrated stability through molecular dynamics simulation and normal mode analysis. While additional in vitro and in vivo studies are needed, the promising data suggest the effectiveness of this vaccine against MPV.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Immunology
Rina Fajri Nuwarda, Abdulsalam Abdullah Alharbi, Veysel Kayser
Summary: Influenza is a major public health concern, and vaccines are an effective method for prevention and control. New vaccine platforms and advancements in vaccine manufacturing processes are being explored to improve effectiveness and stability.
