Article
Immunology
Joanne Marie M. Del Rosario, Kelly A. S. da Costa, Benedikt Asbach, Francesca Ferrara, Matteo Ferrari, David A. Wells, Gurdip Singh Mann, Veronica O. Ameh, Claude T. Sabeta, Ashley C. Banyard, Rebecca Kinsley, Simon D. Scott, Ralf Wagner, Jonathan L. Heeney, George W. Carnell, Nigel J. Temperton
Summary: A library of influenza hemagglutinin (HA) pseudotypes was developed for use in influenza neutralization assays, demonstrating high sensitivity and specificity for detecting virus-specific neutralizing antibodies and assessing antibody functionality. These HA pseudotypes can serve as substitutes for wildtype viruses in experiments and are valuable for evaluating cross-subtype immune responses elicited by vaccines.
Article
Education, Scientific Disciplines
Swarnendu Basak, Hae-Ji Kang, Ki-Back Chu, Judy Oh, Fu-Shi Quan
Summary: A simple, rapid, and accurate plaque assay method using immunostaining was established for visualizing plaques in Sf9 cells infected with recombinant baculoviruses. Variations in cell seeding densities led to differences in final plaque quantification, highlighting the importance of standardizing cell concentrations in baculovirus-related studies.
Article
Biotechnology & Applied Microbiology
Carly M. Bliss, Alec W. Freyn, Tom G. Caniels, Victor H. Leyva-Grado, Raffael Nachbagauer, Weina Sun, Gene S. Tan, Virginia L. Gillespie, Meagan McMahon, Florian Krammer, Adrian V. S. Hill, Peter Palese, Lynda Coughlan
Summary: Conventional influenza vaccines fail to provide broad protection against diverse influenza A viruses. However, this study presents a non-replicating adenoviral vaccine that can elicit broad and cross-reactive protection against multiple strains of influenza A in mice.
Article
Virology
Marwa Arbi, Imen Larbi, Jihen Nsiri, Imen El Behi, Ahmed Rejeb, Khaled Miled, Adeljelil Ghram, Mehdi Houimel
Summary: This study identified new antiviral peptides that can inhibit the infectivity of H9N2 virus by screening a random linear hexapeptide library. Sixteen different phage-peptides were found to specifically bind to H9N2 virus, and two selected peptides (LSRMPK and FAPRWR) showed antiviral activity in both in ovo and in vivo experiments. These peptides prevent infection by inhibiting the attachment of the H9N2 virus to the cellular receptor.
Article
Microbiology
Ki-Hye Kim, Zhuo Li, Noopur Bhatnagar, Jeeva Subbiah, Bo Ryoung Park, Chong Hyun Shin, Peter Pushko, Bao-Zhong Wang, Sang-Moo Kang
Summary: The study developed a universal influenza vaccine candidate that can provide broad cross protection against diverse influenza A and B viruses, and is effective in both young and aged mice.
Article
Multidisciplinary Sciences
Chika Kikuchi, Aristotelis Antonopoulos, Shengyang Wang, Tadashi Maemura, Rositsa Karamanska, Chiara Lee, Andrew J. Thompson, Anne Dell, Yoshihiro Kawaoka, Stuart M. Haslam, James C. Paulson
Summary: The evolution of human H3N2 influenza viruses has led to a limited specificity of the hemagglutinin to a subset of glycan receptors, presenting challenges for virus recognition and vaccine production. By studying glyco-engineered cell lines, the authors demonstrate the importance of extended glycan receptors for the growth of recent H3N2 viruses and their relevance to vaccine production.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Ki-Hye Kim, Zhuo Li, Noopur Bhatnagar, Jeeva Subbiah, Bo Ryoung Park, Chong Hyun Shin, Peter Pushko, Bao-Zhong Wang, Sang-Moo Kang
Summary: This study developed a virus-like particle that can induce immune responses against multiple subtypes of influenza viruses. The vaccination provided broad protection against different strains of influenza A and B viruses. The findings suggest the potential of developing a universal vaccine against influenza in both young and aged populations.
Article
Immunology
Eleni Vatzia, Katherine Feest, Adam McNee, Tanuja Manjegowda, B. Veronica Carr, Basudev Paudyal, Tiphany Chrun, Emmanuel A. A. Maze, Amy Mccarron, Susan Morris, Helen E. E. Everett, Ronan MacLoughlin, Francisco J. J. Salguero, Teresa Lambe, Sarah C. C. Gilbert, Elma Tchilian
Summary: The study found that immunization with chimpanzee adenovirus and modified vaccinia Ankara vaccines expressing conserved influenza virus proteins can provide enhanced immune responses against H3N2 influenza and reduce viral shedding and lung pathology. The results are significant for the development of a broadly protective influenza vaccine and will contribute to future vaccine and clinical trial design.
Article
Chemistry, Analytical
Jiang Qian, Matthew P. Donohue, Thomas Bowen, Ying Zhang
Summary: The potency of inactivated influenzaviral vaccines is currently assessed using the SRID method. However, the time delay in producing the necessary reagents for SRID can be problematic. The LTD-IDMS method, which does not require antisera or reference antigens, has shown comparable results to SRID. In this study, a streamlined improvement to the LTD-IDMS method is demonstrated, saving time and labor in the sample preparation process.
ANALYTICAL CHEMISTRY
(2023)
Review
Immunology
Arun B. Arunachalam, Penny Post, Deborah Rudin
Summary: The RIV4 influenza vaccine, produced through recombinant technology, has distinct post-translational modifications and structural features that result in high levels of protective antibodies. Compared to traditional influenza vaccines, RIV4 has advantages in reducing reliance on virus and process impurities, and improving the breadth of immune responses.
Article
Nanoscience & Nanotechnology
Meital Eckshtain-Levi, Cole J. Batty, Liubov M. Lifshits, Brandon McCammitt, Kathryn M. Moore, Eva A. Amouzougan, Rebeca T. Stiepel, Eliza Duggan, Ted M. Ross, Eric M. Bachelder, Kristy M. Ainslie
Summary: This study demonstrates the potential of a zinc-carnosine metal-organic coordination polymer as a platform for recombinant protein-based vaccines. Compared to traditional zeolitic imidazolate frameworks, zinc-carnosine showed lower cytotoxicity and enhanced innate immune activation and antibody production.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Meital Eckshtain-Levi, Cole J. Batty, Liubov M. Lifshits, Brandon McCammitt, Kathryn M. Moore, Eva A. Amouzougan, Rebeca T. Stiepel, Eliza Duggan, Ted M. Ross, Eric M. Bachelder, Kristy M. Ainslie
Summary: A zinc-carnosine metal-organic coordination polymer was developed as a vaccine platform, which exhibited low cytotoxicity and enhanced innate immune activation. In a mouse model, antigen formulated with zinc-carnosine induced significantly higher levels of specific antibodies and skewed the immune response towards Th1. Furthermore, the antigen formulated with zinc-carnosine increased peptide presentation to T cells and induced cytokine production.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Immunology
Andrew Chak-Yiu Lee, Anna Jinxia Zhang, Can Li, Yanxia Chen, Feifei Liu, Yan Zhao, Hin Chu, Carol Ho-Yan Fong, Pui Wang, Siu-Ying Lau, Kelvin Kai-Wang To, Honglin Chen, Kwok-Yung Yuen
Summary: Intradermal vaccination with DelNS1-LAIV provides potent and long-lasting protection against lethal virus challenge in mice, reducing viral load, epithelial cell death, and inflammatory responses. Activation of antigen-presenting cells and strong immunogenicity suggest that antibody responses play a key role in the protection induced by intradermal DelNS1-LAIV, offering an alternative route for LAIV administration.
Article
Pharmacology & Pharmacy
Yichen Wang, Bo Jin, Bao Li, Yucen Luo, Mengrui Ma, Yongfeng Chen, Hui Liu, Huichao Xie, Tianzhi Yang, Xiaoyun Zhao, Pingtian Ding
Summary: The difficulty faced in siRNA therapeutic application is the lack of safe and effective delivery vectors. Virosomes, composed of lipid membrane and membrane protein, have the potential to deliver protein and nucleic acid drugs. However, traditional virosome preparation methods are limited. This study proposes synthesizing influenza virus hemagglutinin HA2 virosomes using cell-free protein synthesis, paving the way for constructing a novel nano vector with high delivery efficiency and biosafety for siRNA delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Multidisciplinary Sciences
Heni Muflihah, Manuela Florido, Leon C. W. Lin, Yingju Xia, James A. Triccas, John Stambas, Warwick J. Britton
Summary: Pulmonary immunization with recombinant influenza A viruses (rIAVs) expressing M. tuberculosis CD4(+) T cell epitope can enhance protection against lung TB infection. However, sequential pulmonary immunization with rIAVs after BCG did not significantly increase protection against M. tuberculosis infection, despite increasing specific memory T cell responses in the lung.
Review
Immunology
Fang He, Sonja Leyrer, Jimmy Kwang
EXPERT REVIEW OF VACCINES
(2016)
Article
Virology
Yunrui Tan, Qingyong Ng, Qiang Jia, Jimmy Kwang, Fang He
JOURNAL OF VIROLOGY
(2015)
Article
Multidisciplinary Sciences
Subaschandrabose Rajesh Kumar, Mookkan Prabakaran, Kattur Venkatachalam Ashok Raj, Fang He, Jimmy Kwang
Article
Multidisciplinary Sciences
Prashant Rai, Marcus Parrish, Ian Jun Jie Tay, Na Li, Shelley Ackerman, Fang He, Jimmy Kwang, Vincent T. Chow, Bevin P. Engelward
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Review
Virology
Qingyong Ng, Fang He, Jimmy Kwang
Article
Pharmacology & Pharmacy
Fang He, Mookkan Prabakaran, Subaschandrabose Rajesh Kumar, Yunrui Tan, Jimmy Kwang
ANTIVIRAL RESEARCH
(2014)
Article
Multidisciplinary Sciences
Mookkan Prabakaran, Sonja Leyrer, Fang He, Sebastian Auer, Subaschandrabose R. Kumar, Kathrin Kindsmueller, Nutan Mytle, Joerg Schneider, Stephen Lockhart, Jimmy Kwang
Article
Multidisciplinary Sciences
Prashant Rai, Fang He, Jimmy Kwang, Bevin P. Engelward, Vincent T. K. Chow
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Qiang Jia, Qingyong Ng, Wenjie Chin, Tao Meng, Vincent Tak Kwong Chow, Cheng-I Wang, Jimmy Kwang, Fang He
SCIENTIFIC REPORTS
(2017)
Article
Virology
Ze-Hui Liu, Kai-Xia Lei, Guang-Wei Han, Hui-Ling Xu, Fang He
Article
Immunology
Li-Na Tao, Ze-Hui Liu, Hui-Ling Xu, Ying Lu, Min Liao, Fang He
Article
Immunology
Huiling Xu, Zehui Liu, Suya Zheng, Guangwei Han, Fang He
Article
Immunology
Ze-Hui Liu, Hui-Ling Xu, Guang-Wei Han, Li-Na Tao, Ying Lu, Su-Ya Zheng, Wei-Huan Fang, Fang He
Summary: This study developed a novel vaccine strategy to display CSFV E2 glycoprotein on self-assembling NPs, resulting in significant immunogenic effects in pigs, including enhanced antigen uptake and up-regulated production of immunostimulatory cytokines in antigen presenting cells (APCs).
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Rong Chen, Xiao Han, Hankun Xu, Jidong Xu, Tong Cao, Ying Shan, Fang He, Weihuan Fang, Xiaoliang Li
Summary: The expression of histone deacetylase 1 (HDAC1) is reduced during classical swine fever virus (CSFV) infection, and HDAC1 acts as a negative regulator of CSFV replication by activating specific immune pathways. The N-protein of CSFV interacts with transcriptional regulator Sp1 and promotes its degradation, leading to the downregulation of HDAC1 expression. This study provides insights into the mechanisms by which CSFV evades host innate immune responses.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhuofan Deng, Shengkun Zhang, Meiqi Sun, Haotian Yang, Ying Lu, Maopeng Wang, Weihuan Fang, Fushan Shi, Fang He
Summary: In this study, a phage display VHH library was constructed to select nanoantibodies against porcine CD163 receptor. Seven CD163-specific nanoantibodies were identified and showed strong affinity to CD163 receptor and excellent antiviral activity. The nanoantibodies mainly exerted antiviral functions by interfering with virus attachment stage and decreasing the transcription of CD163. The findings provide insights into the biofunction of CD163 in antiviral infection and the development of broad-spectrum strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
