Review
Biotechnology & Applied Microbiology
Rebecca Powell, Despoina Eleftheriadou, Simon Kellaway, James B. Phillips
Summary: This review discusses the physical processes by which natural biomaterials mimic the function of the extracellular matrix and regulate cellular behavior, as well as highlights representative cases of controllable cell microenvironments developed through the combination of cell biology and tissue engineering principles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Zhonghan Wang, Hanxiang Le, Yanbing Wang, He Liu, Zuhao Li, Xiaoyu Yang, Chenyu Wang, Jianxun Ding, Xuesi Chen
Summary: The development of interdisciplinary biomedical engineering has made significant breakthroughs in the field of cartilage regeneration. However, cartilage defects are more complex under clinical conditions, especially when injuries occur at specific sites or under inflammatory microenvironments. This review summarizes the strategies for cartilage regeneration at particular sites and discusses the challenges and opportunities for clinical transformation.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Shuo Chen, Lijuan Wang, Lei Yang, Abdus Samad Rana, Chuanglong He
Summary: Organoid is an emerging frontier technology in the field of life science, wherein pluripotent stem cells or tissue-derived differentiated/progenitor cells form 3D structures according to their multi-directional differentiation potential and self-assembly ability. This review summarizes the recent development of engineered biomimetic microenvironments for organoids, including the composition of the matrix for organoid culture and strategies for engineering the microenvironment from biophysical, biochemical, and cellular perspectives. The newly developed monitoring technologies are also reviewed, and a brief conclusion and outlook for future research are presented.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Feng Han, Qingchen Meng, En Xie, Kexin Li, Jie Hu, Qianglong Chen, Jiaying Li, Fengxuan Han
Summary: The incidence of tissue and organ damage caused by various diseases is increasing worldwide. Tissue engineering, using biomaterials with specific structures, has the potential to regenerate or replace damaged tissues and organs. Micro/nano materials, with their ability to mimic the microstructure of tissues, have greatly advanced the field of tissue engineering. This review provides an overview of different types of micro/nanomaterials, their preparation methods and their applications in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Yuriy Snyder, Soumen Jana
Summary: Valvular heart diseases are commonly treated using mechanical or bioprosthetic heart valves, but these valves have limitations. Heart valve tissue engineering offers a potential solution by using scaffolds that can grow with patients and function without the need for lifelong anticoagulation medication.
Article
Materials Science, Multidisciplinary
Tristan Mes, Aurelie Serrero, Hannah S. Bauer, Martijn A. J. Cox, Anton W. Bosman, Patricia Y. W. Dankers, E. W. Meijer
Summary: The main objective of Endogenous Tissue Restoration (ETR) is to restore the functional tissues in cardiovascular applications. The development of biocompatible, biodegradable, and tunable biomaterials has led to the progress in laboratory studies and clinical application of ETR. Supramolecular polymers, with the ability to control mechanical properties and biodegradability independently, offer unique advantages for ETR. This paper provides details on the mechanism of ETR and discusses the potential of supramolecular polymers in clinical applications.
Article
Engineering, Biomedical
Robert P. Accolla, Jia-Pu Liang, Taylor R. Lansberry, Irayme Labrada Miravet, Marlon Loaisiga, Baltasar Lopez Sardi, Cherie L. Stabler
Summary: An oxygen-generating composite material called OxySite is developed by encapsulating calcium peroxide (CaO2) within polydimethylsiloxane and formulating it into microbeads for tissue integration. Material parameters are modulated to study oxygen generation kinetics and cellular applications. In vitro and in vivo experiments demonstrate the improved metabolic activity and function of cells when coencapsulated with optimized OxySite microbeads.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Yuriy Snyder, Soumen Jana
Summary: Current clinical solutions for valvular heart diseases have limitations, but tissue engineering approach using heart valve scaffolds can provide a life-long solution.
PROGRESS IN MATERIALS SCIENCE
(2023)
Review
Automation & Control Systems
Yingge Zhou, Dilshan Sooriyaarachchi, Defu Liu, George Z. Tan
Summary: Advancements in musculoskeletal tissue engineering have been significant, with a focus on creating biomimetic microenvironments resembling native tissues. This paper reviews recent progress in fabrication strategies for biomimetic scaffolds, highlighting the importance of bioinspired materials, biomimetic structures, and biofabrication techniques. The development of hybrid biofabrication technologies for rapid manufacturing of 3D composite constructs is seen as a promising pathway towards efficient bench-to-bedside translation, with future scaffolds expected to possess modulated functions to adapt to dynamic physiological and mechanical environments for long-term regeneration and improved clinical outcomes.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2021)
Review
Engineering, Biomedical
Xu Chen, Qianping Guo, Wei Chen, Wanli Xie, Yunlong Wang, Miao Wang, Tianyan You, Guoqing Pan
Summary: This review highlights the bio-inspired structure colors of photonic crystal (PC) materials, emphasizing their advancement in biosensing, bio-probing, bio-screening, tissue engineering, and more. In addition, it discusses emerging applications based on functional materials and newly developed material engineering technologies, providing insight for developing next generation smart biomedical materials or devices.
ACTA BIOMATERIALIA
(2021)
Review
Biochemical Research Methods
Xinyu Luo, Jiayue Niu, Guanyu Su, Linxi Zhou, Xue Zhang, Ying Liu, Qiang Wang, Ningning Sun
Summary: This article systematically summarizes the use of biomimetic materials in oral medicine, including tooth defects, tooth loss, periodontal diseases, and maxillofacial bone defects. The article reviews various theoretical foundations of biomimetic materials research, summarizes their benefits and limitations, and discusses the challenges and potential of this field.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Review
Materials Science, Paper & Wood
Walid Kamal Abdelbasset, Saud M. Alrawaili, Ahmed M. Osailan, Ahmed Abdelmoniem Ibrahim, Gaber S. Soliman, Ahmed M. Abodonya
Summary: Valvular heart disease presents a clinical challenge, and heart valve tissue engineering offers potential solutions. Polysaccharides as scaffolding biomaterials have widespread applications in HVTE.
Article
Materials Science, Multidisciplinary
Soumen Jana, Federico Franchi, Amir Lerman
Summary: The study developed trilayered microfibrous leaflet substrates using electrospinning to mimic native heart valve leaflets, which were implanted in rats to study their effect on in vivo tissue engineering. The resulting tissue constructs showed well-defined structure and anisotropic tensile properties suitable for withstanding physiological loads, indicating potential for heart valve replacements.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Applied
Payam Baei, Hamed Daemi, Fatemeh Aramesh, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad
Summary: The purpose of cartilage tissue engineering is to create artificial constructs that mimic native tissue in terms of biological functions and mechanical features, with the aim of improving tissue regeneration. Polysaccharides, due to their structural similarity to the cartilage extracellular matrix (ECM), have attracted attention for developing biomimetic materials. The mechanical properties of constructs and the addition of bioactive molecules play crucial roles in cartilage tissue regeneration. This article discusses polysaccharide-based constructs for cartilage regeneration, with a focus on newly developed biomimetic materials, tuning the mechanical properties of constructs, designing carriers loaded with chondroinductive agents, and the development of appropriate bioinks for bioprinting.
CARBOHYDRATE POLYMERS
(2023)
Review
Polymer Science
Yingxuan Zhu, Mengqi Zhang, Qingqing Sun, Xiaofeng Wang, Xiaomeng Li, Qian Li
Summary: Mechanics plays a crucial role in cell function and tissue regeneration, and the mechanical detection of cell mechanics and cellular-scale microenvironments is essential for understanding cell activities affected by physical factors. This review focuses on advanced techniques and applications of cell-scale mechanical detection, including atomic force microscopy, optical tweezer, magnetic tweezer, and traction force microscopy. It also discusses the application of mechanical testing techniques to cell mechanics and tissue engineering scaffolds. The challenges and prospects in this field are highlighted, providing valuable insights into micromechanics in tissue engineering.
Article
Cell & Tissue Engineering
Maria J. J. Hagelaars, Laura Rijns, Patricia Y. W. Dankers, Sandra Loerakker, Carlijn V. C. Bouten
Summary: This study reviews the role of microenvironment in the development of renal tubules and provides insights on how this knowledge can be used in biomaterial-based tubular engineering using computational models. Understanding the complex chemical, physical, and mechanical interactions between cells and their microenvironment is crucial for guiding renal tubulogenesis. The study highlights the importance of a reciprocal interaction between understanding and engineering to effectively regenerate kidney tissue function.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Materials Science, Biomaterials
Elana M. Meijer, Suzanne E. Koch, Christian G. M. van Dijk, Renee G. C. Maas, Ihsan Chrifi, Wojciech Szymczyk, Paul J. Besseling, Lisa Pomp, Vera J. C. H. Koomen, Jan Willem Buikema, Carlijn V. C. Bouten, Marianne C. Verhaar, Anthal I. P. M. Smits, Caroline Cheng
Summary: This study reports the creation of a perfused human macrovessel model using human induced pluripotent stem cell (hiPSC)-derived vascular organoid cells on an electrospun polycaprolactone-bisurea (PCL-BU) 3D scaffold. The cells harvested from the vascular organoids can be cryopreserved and expanded without loss of cell purity and proliferative capacity. The cells show shear stress response and establish a functional barrier that self-restores after a thrombin challenge. The static bioreactor culture results in a biomimetic vascular bi-layer hierarchy under laminar flow.
Article
Biology
Laura C. Van Eyndhoven, Vincent P. G. Verberne, Carlijn V. C. Bouten, Abhyudai Singh, Jurjen Tel
Summary: Using a murine fibroblast reporter model, we investigated the impact of various stochastic and deterministic host-intrinsic factors on early IFN-I responses. We demonstrated the influence of epigenetic drugs on the percentage of responding cells and uncovered transient heritability driving responder fates through mathematical modeling. Additionally, we found that cell density plays a crucial role in dictating responsiveness, similar to quorum sensing, providing insights into cellular decision-making during early IFN-I responses.
Review
Cardiac & Cardiovascular Systems
Dewy C. van der Valk, Aleksandra Fomina, Marcelle Uiterwijk, Carlijn R. Hooijmans, Anat Akiva, Jolanda Kluin, Carlijn V. C. Bouten, Anthal I. P. M. Smits
Summary: This review systematically analyzes the occurrence of calcification in tissue-engineered heart valves and highlights the need for improved analysis and reporting standards. It also emphasizes the importance of further research to understand the risk of calcification compared to current options.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Laura Rijns, Maria J. J. Hagelaars, Joost J. B. van der Tol, Sandra Loerakker, Carlijn V. C. Bouten, Patricia Y. W. Dankers
Summary: Epithelial cysts and organoids are multicellular hollow structures formed by correctly polarized epithelial cells. Control over the effective ligand concentration in synthetic dynamic supramolecular hydrogels can regulate epithelial polarity in 2D and 3D cultures. The results provide insights on engineering synthetic biomaterials for cell and organoid culture.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Dylan Mostert, Ignasi Jorba, Bart G. W. Groenen, Robert Passier, Marie-Jose T. H. Goumans, Huibert A. van Boxtel, Nicholas A. Kurniawan, Carlijn V. C. Bouten, Leda Klouda
Summary: Environmental stiffness is crucial for cell function. Methacrylated human recombinant collagen peptide (RCPhC1-MA) hydrogels are evaluated as a matrix to control 3D microenvironmental stiffness and monitor cardiac cell response. RCPhC1-MA hydrogels can form reproducible stiffness and allow real-time monitoring of extracellular matrix production and cardiomyocyte contractility in the presence of cardiac fibroblasts.
Article
Engineering, Biomedical
Paul J. J. Besseling, Merle M. M. Krebber, Joost O. O. Fledderus, Martin Teraa, Krista den Ouden, Melanie van de Kaa, Petra M. M. de Bree, Aurelie Serrero, Carlijn V. C. Bouten, Patricia Y. W. Dankers, Martijn A. J. Cox, Marianne C. C. Verhaar
Summary: This study aimed to investigate the effects of chronic kidney disease (CKD) on in vivo scaffold breakdown and tissue formation. The results showed that although CKD led to increased vascular calcification, disease-specific graft design may not be necessary for use in CKD patients on dialysis.
APL BIOENGINEERING
(2023)
Article
Engineering, Biomedical
Tom C. L. Bracco Gartner, Ye Wang, Laurynas Leiteris, Iris van Adrichem, Judith Marsman, Marie Jose Goumans, Carlijn V. C. Bouten, Joost P. G. Sluijter, Jaap M. J. den Toonder, Willem J. L. Suyker, Jesper Hjortnaes
Summary: Cardiac fibroblasts in the ever-beating human heart remain quiescent due to the antifibrotic effect of cyclic strain conditions revealed by a novel platform for studying cardiac fibrosis-on-a-chip. This study provides insights into the mechanosensitive pathways and genes involved in the fibrogenic process, which can contribute to the development of new therapies against cardiac fibrosis.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Ethics
Anne-Floor J. de Kanter, Karin R. Jongsma, Carlijn V. C. Bouten, Annelien L. Bredenoord
Summary: Innovations in the field of Regenerative Medicine may soon allow for the replacement and re-growth of healthy tissues after injury or disease. One promising innovation is a regenerative valve implant, made from 'smart' and 'lifelike' materials, that can stimulate the re-growth of a healthy heart valve. However, the ethical implications and conceptual understanding of these materials are still unclear.
SCIENCE AND ENGINEERING ETHICS
(2023)
Article
Multidisciplinary Sciences
Janine Grolleman, Nicole C. A. van Engeland, Minahil Raza, Sepinoud Azimi, Vito Conte, Cecilia M. Sahlgren, Carlijn V. C. Bouten
Summary: Recent experimental evidence suggests that vimentin, an intermediate filament protein, plays a role in regulating cellular mechanical homeostasis. Vimentin-expressing cells adapt their cellular morphology and mechanics to changes in microenvironment stiffness, while vimentin-depleted cells lose this ability on short timescales but regain it on longer timescales. Additionally, vimentin-depleted cells compensate for the loss of vimentin by increasing collagen matrix synthesis and crosslinking.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Maaike Bril, Aref Saberi, Ignasi Jorba, Mark C. van Turnhout, Cecilia M. Sahlgren, Carlijn V. C. Bouten, Albert P. H. J. Schenning, Nicholas A. Kurniawan
Summary: This study presents a dynamic, cell-compatible, and reconfigurable hydrogel-based platform that allows reversible micrometer-scale changes in surface topography of the cellular environment using blue light stimulation. By investigating fibroblast response to controlled geometry actuations, the study reveals that fibroblasts reorganize their nucleus and focal adhesions in response to recurring topographical changes. This dynamic conditioning is associated with long-term maintenance of focal adhesions and epigenetic modifications.
Article
Cell Biology
Dylan Mostert, Janine Grolleman, Mark C. van Turnhout, Bart G. W. Groenen, Vito Conte, Cecilia M. Sahlgren, Nicholas A. Kurniawan, Carlijn V. C. Bouten
Summary: Ventral actin stress fibers (SFs) are a subset of actin SFs that begin and terminate at focal adhesion (FA) complexes. A computational toolbox called SFAlab is introduced to quantify and provide spatial information about ventral SFs in cells, as well as to analyze nuclei, cells, and FA. SFAlab has been validated for accurate ventral SF detection and is robust against user subjectivity and experimental artifacts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Imke Jansen, Hanneke Crielaard, Tamar Wissing, Carlijn Bouten, Frank Gijsen, Ali C. Akyildiz, Eric Farrell, Kim van der Heiden
Summary: The presence of microcalcifications on the surface of atherosclerotic plaques may increase the risk of plaque rupture. In this study, a novel tissue-engineered model was developed to mimic atherosclerotic caps with microcalcifications and assess their impact on cap mechanics. The results showed that caps with microcalcifications had lower stiffness and ultimate tensile stress, indicating an increased risk of rupture in atherosclerotic plaques with microcalcifications.
APL BIOENGINEERING
(2023)
Review
Cardiac & Cardiovascular Systems
Valentine C. Vetter, Carlijn V. C. Bouten, Atze van der Pol
Summary: This review focuses on the use of hydrogels for supporting post-myocardial infarcted tissue. It provides insights into crucial design considerations and their implications for future clinical use.
CURRENT HEART FAILURE REPORTS
(2023)
Article
Biology
Annemijn Vis, Bente J. de Kort, Wojciech Szymczyk, Jan Willem van Rijswijk, Sylvia Dekker, Rob Driessen, Niels Wijkstra, Paul F. Grundeman, Hans W. M. Niessen, Henk M. Janssen, Serge H. M. Sontjens, Patricia Y. W. Dankers, Anthal I. P. M. Smits, Carlijn V. C. Bouten, Jolanda Kluin
Summary: Pliable microfibrous, bioresorbable elastomeric heart valve prostheses were studied for sustainable heart valve replacement. In an in vivo study in sheep, these cell-free implants demonstrated good functionality and tissue formation, although some variability and defects were observed. Further research is needed to determine the optimal heart valve prosthesis.
COMMUNICATIONS BIOLOGY
(2023)
