Review
Engineering, Biomedical
Lillian Yuxian Lim, Shirley Suet Lee Ding, Padmalosini Muthukumaran, Swee Hin Teoh, Yexin Koh, Adrian Kee Keong Teo
Summary: Pancreas or islet transplantation is the reliable treatment for Type I diabetes patients, but the shortage of viable pancreata limits its use. Organ decellularization and recellularization offer a potential alternative source of donor organs. Studies on decellularization and recellularization of rodent, porcine, and human pancreata show promise for generating usable decellularized pancreas scaffolds. This review discusses the latest advances in pancreas decellularization, recellularization, and revascularization, as well as clinical considerations and future work needed for clinical translation of bioengineered pancreata.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Alberto Bruning Guimaraes, Aristides Tadeu Correia, Ronaldo Soares da Silva, Elizabete Silva dos Santos, Natalia de Souza Xavier Costa, Marisa Dolhnikoff, Marina Maizato, Idagene Aparecida Cestari, Paulo Manuel Pego-Fernandes, Paulo Francisco Guerreiro Cardoso
Summary: Tracheal replacement with bioengineered substitutes has been developed for long-segment tracheal diseases. The study investigated the effect of different storage protocols on the biomechanical properties of decellularized tracheal scaffolds. The results showed that the storage protocols did not significantly affect the collagen content and biomechanical properties of the scaffolds.
BIOENGINEERING-BASEL
(2023)
Review
Oncology
Jianghao Ren, Yuanyuan Xu, Guo Zhiyi, Ting Ren, Jiangbin Ren, Kan Wang, Yiqing Luo, Mingyang Zhu, Qiang Tan
Summary: There have been significant advancements in medical techniques, but the treatment of tracheal defects still faces challenges and lacks consensus. Surgical outcomes vary in individuals, leading to an ambiguous future for tracheal surgery. Bioengineering is seen as an alternative for tracheal defect treatment, but is limited by material shortages and ethical controversies.
Review
Biotechnology & Applied Microbiology
Astgik Petrosyan, Paulo. N. N. Martins, Kim Solez, Basak. E. E. Uygun, Vijay. S. S. Gorantla, Giuseppe Orlando
Summary: Insights into the use of cellular therapeutics, extracellular vesicles (EVs), and tissue engineering strategies for regenerative medicine applications are continually emerging. These approaches have shown strong potential in modulating inflammatory immune responses, promoting regeneration in diseased organs, and treating inflammatory diseases like COVID-19. The field of stem cell therapies, in particular, has seen significant advancements in understanding stem cell heterogeneity and dosage specificity, leading to expanded clinical applications. Standardizing manufacturing protocols for tissue-engineered grafts is a crucial step towards whole organ engineering. Despite challenges, the prospects for personalized regenerative and transplant medicine are promising.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Hiroo Sasaki, Hidetaka Suga, Kazuhito Takeuchi, Yuichi Nagata, Hideyuki Harada, Tatsuma Kondo, Eiji Ito, Sachi Maeda, Mayu Sakakibara, Mika Soen, Tsutomu Miwata, Tomoyoshi Asano, Hajime Ozaki, Shiori Taga, Atsushi Kuwahara, Tokushige Nakano, Hiroshi Arima, Ryuta Saito
Summary: We have successfully developed pituitary organoids (POs) derived from human embryonic stem cells (hESC). Transplantation of these POs into hypopituitary severe combined immunodeficient (SCID) mice was able to effectively restore ACTH secretion, thus providing a potential treatment for hypopituitarism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Surgery
Hisanobu Higashi, Hiroshi Yagi, Kohei Kuroda, Kazuki Tajima, Hideaki Kojima, Kotaro Nishi, Toshinori Morisaku, Kazuya Hirukawa, Kazumasa Fukuda, Kentaro Matsubara, Minoru Kitago, Masahiro Shinoda, Hideaki Obara, Shungo Adachi, Kumiko Nishimura, Tohru Natsume, Masatoshi Tomi, Alejandro Soto-Gutierrez, Yuko Kitagawa
Summary: This study engineered a human-scale transplantable liver graft using a porcine liver-derived scaffold and evaluated its performance for 28 days using a preclinical large animal model. The results showed improvement in liver function after transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Engineering, Biomedical
Sanaz Behtaj, Fereshteh Karamali, Samaneh Najafian, Elahe Masaeli, Mohammad-Hossein Nasr Esfahani, Maksym Rybachuk
Summary: Stem cell-based retinal ganglion cells (RGCs) replacement therapy shows potential for restoring vision in glaucoma by replacing degenerated RGCs and facilitating axonal regeneration. However, generating clinically safe and functional RGCs that can integrate into hosts' retinas remains a challenge. The use of PGS/PCL scaffold in differentiation of hESCs into RGC-like cells promotes cell differentiation and neurite growth, contributing to potential future clinical therapy for glaucoma treatment.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Zhihao Wang, Fei Sun, Yi Lu, Boyou Zhang, Guozhong Zhang, Hongcan Shi
Summary: By utilizing vacuum assistance and optimizing the concentration of DNase I, this study successfully developed a rapid method for preparing decellularized tracheal scaffolds, which demonstrated significant improvements in cell compatibility and in vivo experiments.
Review
Engineering, Biomedical
Motaharesadat Hosseini, Karl R. Koehler, Abbas Shafiee
Summary: Current models for generating human skin organs lack appendages, posing a challenge for engineering skin with all components. Researchers are striving to regenerate skin organs with appendages using bioengineering approaches.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Kenny Man, Neil M. Eisenstein, David A. Hoey, Sophie C. Cox
Summary: In the past decade, extracellular vesicles (EVs) have emerged as key regulators of bone development, homeostasis and repair. Due to their innate biocompatibility, low immunogenicity, and high physiochemical stability, these nanoparticles have garnered interest as potential nanoscale therapeutics. However, there are challenges in the EV supply chain that hinder their clinical translation. This review explores bioengineering strategies to maximize the therapeutic potential of EVs for bone repair.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Cell Biology
Lingfei Zhao, Chenxia Hu, Fei Han, Dajin Chen, Yanhong Ma, Fanghao Cai, Jianghua Chen
Summary: The advancement of organ preservation techniques aims not only to increase the availability of organs for solid organ transplantation, but also to simulate an ex vivo physiological microenvironment. The combination of mesenchymal stromal cells with machine perfusion has shown promising results compared to traditional preservation methods. This strategy helps improve organ function, utilize previously deemed untransplantable organs, and address the issue of organ shortage in the field of solid organ transplantation.
CELL AND TISSUE RESEARCH
(2021)
Review
Cell & Tissue Engineering
Umber Cheema
Summary: Tissue engineering is a multidisciplinary approach to building 3D human tissues in the laboratory. Despite three decades of advancements in medical sciences, there is limited use of tissue-engineered organs in humans. This position paper outlines the challenges and advancements in engineering specific tissues and organs, as well as the most successful technologies in tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Aparna Murali, Giriraj Lokhande, Kaivalya A. Deo, Anna Brokesh, Akhilesh K. Gaharwar
Summary: Two-dimensional nanomaterials show unique physical, chemical, and biological properties, allowing for enhanced interactions with biomolecules and cells, and have broad potential in biomedical applications.
Article
Emergency Medicine
Courtney Premer, Ivonne H. Schulman, Jennifer S. Jackson
Summary: MSCs show promising potential in treating acute diseases, with clinical trials demonstrating safety but inconsistent efficacy data, necessitating further research.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2021)
Review
Anatomy & Morphology
Fatemeh Khazaei, Leila Rezakhani, Morteza Alizadeh, Elahe Mahdavian, Mozafar Khazaei
Summary: Exosomes are extracellular vesicles derived from endosomes that have gained attention for their potential use in regenerative therapies and tissue repair. However, their poor bioavailability and lack of controlled-release mechanisms have been addressed by scaffolding them with biomaterials to create Exosomes-loaded scaffolds (ELS). These biomaterial-based scaffolds can be designed and functionalized to improve the bioavailability, biocompatibility, and controlled release of Exosomes.