Article
Biochemistry & Molecular Biology
Rei-Hsing Hu, Chun-Wen Cheng, Chia-Ta Wu, Jiunn-Liang Ko, Ko-Huang Lue, Yu-Fan Liu
Summary: House dust mites (HDMs) are a significant cause of allergies and asthma. In central Taiwan, approximately 80% of individuals are sensitized to a particular mite species called Dermatophagoides microceras (Der m). This sensitivity is related to the immune response to other mite species. A study used integrated OMICs examination to identify and characterize a specific allergen component, Der m 1. The study found that Der m 1 is a cysteine protease and plays a role in the digestion of fibrinogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Marc Y. Fink, Xiulan Qi, Kari Ann Shirey, Rachel Fanaroff, Svetlana Chapoval, Rose M. Viscardi, Stefanie N. Vogel, Achsah D. Keegan
Summary: Asthma is a chronic respiratory disease associated with airway inflammation and hyperreactivity. The TLR4 gene polymorphisms have been shown to be associated with a decreased risk for asthma, but the interaction with environmental factors is still unclear.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Lin Yang, Yaqi Yang, Qingxiu Xu, Wei Zhang, Qing Jiang, Wenjing Li, Yin Wang, Dongxia Ma, Xiaomin Lin, Baoqing Sun, Rongfei Zhu
Summary: Allergen immunotherapy can induce the production of sIgG4 to house dust mite components, but the increased sIgG4 levels do not correlate with the corresponding sIgE levels or AIT response, thus sIgG4 is not a useful biomarker for evaluating the efficacy of AIT.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Allergy
Mariana Farraia, Ines Paciencia, Francisca Castro Mendes, Joao Cavaleiro Rufo, Mohamed H. Shamji, Ioana Agache, Andre Moreira
Summary: This study compared the cost-effectiveness of allergen immunotherapy (AIT) to standard treatment in children with allergic asthma. The results showed that AIT, especially when administered subcutaneously, was cost effective in reducing asthma exacerbations and medication use.
Article
Construction & Building Technology
Yuexia Sun, Lianwang Cui, Jing Hou, Shugang Luo, Dan Norback, Jan Sundell
Summary: This study investigated the exposure level of house dust mite allergens in northeast China and found that high indoor humidity, low air change rate, indoor dampness, and frequent changing of bedding were significantly associated with high allergen concentration. The study also found that high ventilation rate can reduce house dust mite allergens, possibly due to the decrease in indoor-outdoor humidity gradient.
Article
Allergy
Huey-Jy Huang, Yvonne Resch-Marat, Anne Casset, Margit Weghofer, Petra Zieglmayer, Rene Zieglmayer, Patrick Lemell, Friedrich Horak, Kuan-Wei Chen, Ekaterina Potapova, Paolo M. Matricardi, Gabrielle Pauli, Monika Grote, Rudolf Valenta, Susanne Vrtala
Summary: Der p 37, a new Dp allergen recognized by a third of HDM-allergic patients, may serve as a surrogate marker for severe HDM sensitization and asthma.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
Mirela Curin, Huey-Jy Huang, Tetiana Garmatiuk, Sandra Gutfreund, Yvonne Resch-Marat, Kuan-Wei Chen, Kerstin Fauland, Walter Keller, Petra Zieglmayer, Rene Zieglmayer, Patrick Lemell, Friedrich Horak, Wolfgang Hemmer, Margarete Focke-Tejkl, Sabine Flicker, Susanne Vrtala, Rudolf Valenta
Summary: The study found that Der p 7 has high allergenic activity comparable to other allergens, but did not show any IgE reactivity or allergenic activity in HDM-allergic patients, indicating a lack of sequential IgE epitopes on Der p 7. Through IgE inhibition experiments and molecular modeling, discontinuous, conformational IgE epitopes of Der p 7 were identified.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Andrea N. Loes, Melissa N. Hinman, Dylan R. Farnsworth, Adam C. Miller, Karen Guillemin, Michael J. Harms
Summary: The zebrafish is a powerful model organism for studying the innate immune system. Recent findings suggest that zebrafish share an LPS-sensitive Tlr4/Md-2 complex with mammals, rather than a new invention in tetrapods. The discovery of the Ly96 gene in zebrafish and functional assays further support this hypothesis, indicating a low-sensitivity ancestral Tlr4/Md-2 complex retained by zebrafish.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Rei-Hsing Hu, Chia-Ta Wu, Ting-Shuan Wu, Feng-Yih Yu, Jiunn-Liang Ko, Ko-Huang Lue, Yu-Fan Liu
Summary: This study identified and characterized the allergen-like protein Der m 2 in the indoor house dust mite Dermatophagoides microceras (Der m). Der m 2 was found to induce inflammatory responses in cell and mouse models of allergic asthma. Treatment with the fungal immunomodulatory protein FIP-fve alleviated the inflammation caused by Der m 2-induced asthma in mice.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jose Cristian Vidal-Quist, Jozefien Declercq, Stijn Vanhee, Bart N. Lambrecht, Jose Gomez-Rial, Carmen Vidal, Eylem Aydogdu, Stephane Rombauts, Pedro Hernandez-Crespo
Summary: This study evaluated the impact of viral infection on the physiological characteristics and allergenicity of Dermatophagoides pteronyssinus. Transcriptional changes and allergen accumulation were observed, but the impact on allergic asthma and human IgE binding was not significant. Overall, viral infection did not significantly affect the allergenic performance of extracts or their practical use in allergy diagnosis.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thierry Batard, Walter G. Canonica, Oliver Pfaar, Mohamed H. Shamji, Robyn E. O'Hehir, Menno C. van Zelm, Laurent Mascarell
Summary: Allergy to house dust mites (HDM) is a common respiratory disease that affects over 500 million people worldwide. The two main species of HDM, Dermatophagoides pteronyssinus and D. farinae, are major sources of indoor allergens that trigger allergic inflammation. While symptomatic drugs can help alleviate symptoms, allergen immunotherapy is the only curative treatment for IgE-mediated type I respiratory allergies. This article reviews recent advances in various routes of immunotherapy, with a focus on subcutaneous (SCIT) and sublingual (SLIT) immunotherapy, which have transformed allergic treatments by improving symptoms and quality of life for patients.
MOLECULAR IMMUNOLOGY
(2023)
Article
Allergy
Alexander Heldner, Francesca Alessandrini, Dennis Russkamp, Sonja Heine, Benjamin Schnautz, Adam Chaker, Juliet Mwange, Thalia L. Carreno Velazquez, Matthew D. Heath, Murray A. Skinner, Matthias F. Kramer, Ulrich M. Zissler, Carsten B. Schmidt-Weber, Simon Blank
Summary: This study demonstrated that low-dose allergoid AIT had comparable efficacy to suppress allergic airway inflammation and Th2-type cytokine secretion as high-dose extract AIT, but was less effective in inducing a potentially protective IgG1 response. Combining low-dose allergoid AIT with MCT or MCT and dose-adjusted MPL promoted Th1-inducing mechanisms and robust B-cell activation to counterbalance the allergic Th2 immune response. Conclusion: Low allergen doses induce cellular and humoral mechanisms counteracting Th2-driven inflammation by using allergoids and dose-adjusted adjuvants.
Article
Allergy
Magdalena Zemelka-Wiacek, Anna Kosowska, Ewa Winiarska, Ewa Sobanska, Marek Jutel
Summary: This study aimed to demonstrate the reproducibility of defined clinical endpoints after AEC house dust mite (HDM) challenge under optimal conditions. The results showed that in a constant environment, the concentration of HDM remained stable within the targeted values, and the severity of symptoms and other endpoints were reproducible. A concentration of 5000 particles/m³ and a challenge duration of 120 minutes were found to be optimal.
Article
Cell Biology
Karin Pfisterer, Matthias Wielscher, David Samardzic, Pauline Weinzettl, Dorte Symmank, Lisa E. Shaw, Raffaela Campana, Huey-Jy Huang, Matthias Farlik, Christine Bangert, Susanne Vrtala, Rudolf Valenta, Wolfgang Weninger
Summary: The allergen Der p 2 and its peptides can penetrate skin cells, leading to skin inflammation, barrier dysfunction, and hyperproliferation of keratinocytes, which contribute to the initiation and exacerbation of atopic dermatitis (AD).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alessio Borio, Aurora Holgado, Christina Passegger, Herbert Strobl, Rudi Beyaert, Holger Heine, Alla Zamyatina
Summary: The TLR4/MD-2 complex is a crucial receptor in the innate immune system and plays a major role in inflammation, but dysregulation in its signaling can lead to uncontrolled inflammation and disease. Targeting this receptor complex with anti-inflammatory therapies is promising, but the complexity of ligand recognition and species-specificity presents challenges. Researchers have developed glycolipids that mimic lipid A and inhibit TLR4-mediated inflammation, and these glycolipids were evaluated for their activity in human and murine immune cells. A bis-phosphorylated glycolipid was found to have antagonist activity on human TLR4, but acted as a partial agonist on murine TLR4.