Article
Infectious Diseases
Reo Onishi, Katsumi Shigemura, Kayo Osawa, Young-Min Yang, Koki Maeda, Shiuh-Bin Fang, Shian-Ying Sung, Kenichiro Onuma, Atsushi Uda, Takayuki Miyara, Masato Fujisawa
Summary: Imipenemase-6 (IMP-6) type carbapenemase-producing Enterobacteriaceae is dangerous due to its unique lack of susceptibility to antimicrobial agents, specifically being resistant to meropenem but susceptible to imipenem. In addition to carbapenemase, outer membrane porins and efflux pumps also contribute to carbapenem resistance. The presence of broad-spectrum beta-lactamase (ESBL), transmitted via plasmid, further broadens the spectrum of antimicrobial resistance. Decreased porin expression is a possible reason for the non-susceptibility of 50% of IMP-6-producing strains to imipenem.
Article
Engineering, Environmental
Ana Puljko, Milena Milakovic, Stela Krizanovic, Josipa Kosic-Vuksic, Ivana Babic, Ines Petric, Ana Maravic, Marko Jelic, Nikolina Udikovic-Kolic
Summary: Extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Enterobacterales are still present in the environment even after conventional treatment in wastewater treatment plants. Enteric opportunistic pathogens such as Klebsiella pneumoniae and Acinetobacter baumannii are efficiently removed, but Escherichia coli and Enterococcus spp. still remain in relatively high concentrations in the effluent. Improvement in wastewater treatment technologies is necessary to minimize the risk of environmental contamination and public health issues related to ESBL and carbapenemase-producing Enterobacterales.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Letter
Evolutionary Biology
Ward C. Wheeler
Summary: The treatment of inapplicable characters has been a challenging problem for systematists. Researchers have tried different coding scenarios to capture the presence and absence of a feature, as well as its variation. The approach of treating presence/absence as insertion/deletion of a character removes three problems in a simple and straightforward manner.
Review
Microbiology
Karl A. Glen, Iain L. Lamont
Summary: The article summarizes the resistance of Pseudomonas aeruginosa to beta-lactam antibiotics and the associated health and economic impacts, discusses the multifactorial mechanisms of resistance, and suggests strategies to enhance the effectiveness of beta-lactam antibiotics.
Article
Computer Science, Information Systems
Yubo Sun, Yuanxiao Xi, Gennian Ge
Summary: Motivated by applications in modern storage devices, this paper studies the sequence reconstruction problem by determining the maximum intersection size between two error balls and designing reconstruction codes. It focuses on channels with single-burst-insertion/deletion errors and fully determines the maximum intersection size for both fixed-length and variable-length models. Characterizations of sequence pairs with a certain intersection size are used to design reconstruction codes and analyze their lower bounds. The results are extended to the burst-edit channel.
IEEE TRANSACTIONS ON INFORMATION THEORY
(2023)
Article
Microbiology
Suna Sibel Gurpinar, Didem Kart, Mujde Eryilmaz
Summary: This study investigates the effects of antidepressants on the development of antibiotic resistance in clinical Acinetobacter baumannii isolates. The study found that these antidepressants can increase antibiotic resistance and affect gene expression levels.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Nicolas Cabanel, Isabelle Rosinski-Chupin, Adriana Chiarelli, Tatana Botin, Marta Tato, Rafael Canton, Philippe Glaser
Summary: Outbreak of carbapenemase-producing Klebsiella pneumoniae poses a significant threat to hospitals. The molecular characterization of the first outbreak of VIM-1-producing K. pneumoniae in Spain revealed the clonality of isolates and the acquisition of resistance genes and mutations. The spread of the CPKp strain was not observed, but the VIM-1 plasmid was found in various Enterobacterales species, predominantly in Spain. Additionally, transposition of an IS element into the ureC gene was identified as a cause for urease loss in K. pneumoniae isolates.
Article
Microbiology
Yizhi Tang, Yan Lai, Xingyuan Wang, Changwei Lei, Chao Li, Linghan Kong, Yulong Wang, Hongning Wang
Summary: A novel transposon ISChh1-like was identified in Campylobacter, mediating acquisition of multiple antibiotic resistance genes, including the important optrA gene.
VETERINARY MICROBIOLOGY
(2021)
Review
Multidisciplinary Sciences
Hannah R. Noel, Jessica R. Petrey, Lauren D. Palmer
Summary: Pathogenic Acinetobacter species, especially Acinetobacter baumannii, are a major cause of healthcare-associated infections worldwide. These infections are of great concern due to their high levels of multidrug resistance and extensive drug resistance. Genome sequencing and analysis have revealed that bacterial antibiotic resistance is often acquired and spread through the movement of mobile genetic elements.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Microbiology
Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien
Summary: Our study aimed to investigate the effects of deleting tolC on the adhesion and invasion of HT-29 epithelial cells and THP-1 macrophages by S. Typhi in vitro. Our results showed that the tolC mutant strain had significantly reduced adhesion and invasion rates compared to the wild-type strain in both cell types. Additionally, we observed downregulation of SPI-1 gene expression in the tolC mutant.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Lili Chu, Shengqiang Ye, Jiaying Wang, Duo Peng, Xiaoyan Wang, Yunguo Qian, Dabing Zhang
Summary: Duck adenovirus 3 (DuAdV-3; strain HB) isolated in this study is a mutant with several insertion and deletion events in its genome, demonstrating genetic variation among DuAdV-3 isolates.
ARCHIVES OF VIROLOGY
(2022)
Article
Microbiology
Stephen Mark Edward Fordham, Anna Mantzouratou, Elizabeth Sheridan
Summary: Colistin is a last resort antibiotic for treating carbapenemase producing Klebsiella pneumoniae. The insertion of IS elements into the mgrB gene is a mechanism that causes colistin resistance. Plasmids carrying IS elements are common in K. pneumoniae, with ISKpn25 being mostly carried by IncFIB(pQil) plasmids, while ISKpn26 and ISKpn14 are often associated with IncFII(pHN7A8) plasmids. ISKpn25 is identified in 26 out of 34 countries that contain any of the IS elements, while ISKpn26, ISKpn14, and IS903B are most frequently found in China. Plasmids carrying ISKpn25, ISKpn14, and ISKpn26 have a higher count of carbapenemase genes compared to IS903B-carrying plasmids. IS903B-bearing plasmids have a higher count of environmental source isolations compared to ISKpn25, ISKpn14, and ISKpn26 bearing plasmids. The presence of carbapenemase in combination with IS elements could lead to an extensively drug-resistant profile in K. pneumoniae, limiting treatment options.
Article
Biotechnology & Applied Microbiology
Reo Onishi, Katsumi Shigemura, Kayo Osawa, Young-Min Yang, Koki Maeda, Hiroshi Tanimoto, Mitsuki Kado, Shiuh-Bin Fang, Masato Fujisawa
Summary: This study analyzed the molecular mechanisms of carbapenem resistance in CRKP collected from medical institutions in Hyogo Prefecture, Japan. The main causes of resistance were the production of carbapenemase (IMP-6) in carbapenemase-producing strains and the mutation of ompK35 in non-carbapenemase-producing strains.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Infectious Diseases
Yee Qing Lee, Sasheela Sri La Sri Ponnampalavanar, Chun Wie Chong, Rina Karunakaran, Kumutha Malar Vellasamy, Kartini Abdul Jabar, Zhi Xian Kong, Min Yi Lau, Cindy Shuan Ju Teh
Summary: This study evaluated the clinical and molecular profiles of non-carbapenemase-producing carbapenem-resistant Klebsiella pneumoniae (NC-CRKP) isolated from patients in a Malaysian hospital. The findings showed that some strains of NC-CRKP achieved carbapenem resistance through a combination of porin loss and non-carbapenemase resistance mechanisms.
Article
Biology
Jose F. Delgado-Blas, Cristina M. Ovejero, Sophia David, Natalia Montero, William Calero-Caceres, M. Pilar Garcillan-Barcia, Fernando de la Cruz, Maite Muniesa, David M. Aanensen, Bruno Gonzalez-Zorn
Summary: The study found that bacteria in wastewater are more resistant to antibiotics and primarily adapt through a diverse set of plasmids, whereas bacteria in rivers mainly adapt through chromosomal changes.
COMMUNICATIONS BIOLOGY
(2021)