Article
Biochemistry & Molecular Biology
John A. A. Miller, Domenica E. E. Drouet, Leonid M. M. Yermakov, Mahmoud S. S. Elbasiouny, Fatima Z. Z. Bensabeur, Michael Bottomley, Keiichiro Susuki
Summary: The calpain-calpastatin system plays an important role in myelination and demyelinating diseases of peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
David Aluja, Sara Delgado-Tomas, Marisol Ruiz-Meana, Jose A. Barrabes, Javier Inserte
Summary: Despite advances in treatment, heart failure remains a major cause of illness and death. Preventing myocardial hypertrophy, which is an independent risk factor for heart failure, is a clinical objective. Recent studies suggest that calpains, a type of protein, play a role in the development of cardiac hypertrophy, and inhibiting calpains may be a potential pharmacological strategy. New compounds are being developed and tested in animal models to evaluate the effectiveness of calpain inhibition in treating pathological cardiac hypertrophy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Hong Nian, Binyun Ma
Summary: The calpain system plays crucial roles in cancer progression by mediating the proteolysis of specific substrates and regulating important signaling molecules. mu-calpain, m-calpain, and calpastatin are the three molecules initially identified as components of the calpain system, with roles in cancer progression through regulation of cellular processes and potential as targets for anti-cancer interventions.
BIOLOGICAL REVIEWS
(2021)
Article
Physiology
Elizabeth A. Schroder, Lin Wang, Yuan Wen, Leigh Ann P. Callahan, Gerald S. Supinski
Summary: This study is the first to investigate the effects of calpastatin overexpression on skeletal muscle specific force in aging rodents. Muscle-specific overexpression of calpastatin, the endogenous inhibitor of calpain, prevented aging-induced reductions in both EDL and diaphragm specific force and significantly increased lifespan. These findings suggest that diaphragm dysfunction in aging may play a crucial role in determining longevity, and targeting the calpain/calpastatin pathway could lead to novel therapies for combating skeletal muscle weakness in the elderly.
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Neurosciences
Ruiyang Zhao, Xiufei Teng, Yanchao Yang
Summary: Hypoxic-ischemic encephalopathy (HIE) is a complex process involving inflammation, oxidative stress, and glucose metabolism, leading to brain damage and impairment. Calpain, a family of Ca2+-dependent cysteine proteases, is involved in cerebral ischemic injury and plays a role in neuronal necrosis and apoptosis pathways. This study reviews the potential role of calpain in treating HIE and provides insights for future research.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irina M. Kotova, Nina S. Pestereva, Dmitrii S. Traktirov, Margarita T. Absalyamova, Marina N. Karpenko
Summary: Calpain and calpastatin are key components of the calcium-dependent proteolytic system. Calpains are regulatory, calcium-dependent, cytoplasmic proteinases, while calpastatin is the endogenous inhibitor of calpains. This review summarizes existing data on cerebral calpain distribution and function throughout mammalian ontogenesis, focusing on recent studies and the involvement of the calpain-calpastatin system in normal CNS development and functioning. The activity and production of calpain and calpastatin in different brain regions during ontogenesis are also discussed, as comparative analysis of these results can reveal brain regions and developmental stages with pronounced calpain system function.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Article
Food Science & Technology
Manting Du, Xin Li, Dequan Zhang, Zheng Li, Chengli Hou, Chi Ren, Yanhong Bai
Summary: The study found that phosphorylation catalyzed by protein kinase A at Ser 649 positively regulates the inhibitory abilities of calpastatin by promoting the interaction between calpastatin and calpain, contributing to enrich the regulatory mechanism of mu-calpain activity during post-mortem meat tenderization.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hayden W. Hyatt, Mustafa Ozdemir, Toshinori Yoshihara, Branden L. Nguyen, Rafael Deminice, Scott K. Powers
Summary: Mechanical ventilation is a life-saving intervention for critically ill patients, but it can lead to ventilator-induced diaphragm dysfunction (VIDD) due to oxidative stress activating major proteolytic systems. Calpain activation plays an essential role in VIDD development, leading to oxidative stress, muscle atrophy, and contractile dysfunction.
Article
Agriculture, Dairy & Animal Science
Annie Basson, Phillip E. Strydom, Este Van Marle-Koster, Edward C. Webb, Lorinda Frylinck
Summary: In the search for tender beef, minimizing environmental variations pre-slaughter to post-ageing is crucial for accurate genetic markers. The ageing process plays a significant role in determining if genetic markers are associated with tenderization. Specifically focusing on candidate genes like calpains is important for enhancing tenderness in beef production.
Review
Biochemistry & Molecular Biology
Piotr Kostusiak, Jan Slosarz, Marcin Golebiewski, Grzegorz Grodkowski, Kamila Puppel
Summary: The SNP form of genes provides valuable information for assessing their suitability as specific markers for desirable traits in beef cattle breeding. Previous research has focused on SNP in myostatin, thyroglobulin, calpain, and calpastatin proteins. The literature review highlights the importance of these genes in beef cattle production and suggests their potential to positively influence productivity and production quality.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Immunology
Shinobu Goto, Yasuhiko Ozaki, Fumiko Ozawa, Eita Mizutani, Tamao Kitaori, Nobuhiro Suzumori, Klas Blomgren, Tadahide Furuno, Mayumi Sugiura-Ogasawara
Summary: The study revealed that activation of mu-calpain is suppressed in FGR placentas, and calpain-6 in human placenta is involved in the pathology of FGR.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Chanporn Chaosap, Panneepa Sivapirunthep, Ronachai Sitthigripong, Piyada Tavitchasri, Sabaiporn Maduae, Tipyaporn Kusee, Jutarat Setakul, Kazeem Adeyemi
Summary: This study investigated the differences in meat quality characteristics among different muscles of Thai native cattle by examining the myosin heavy chain (MyHC) isoforms, suggesting that the variation in meat quality may be partly related to the differences in MyHC isoforms.
Review
Biochemistry & Molecular Biology
Micah W. W. Yoder, Nathan T. T. Wright, Maegen A. A. Borzok
Summary: The intercalated disk in the heart is a structure composed of adherens junctions, desmosomes, and gap junctions, which provide mechanical stability and electrical synchronization. Calpain proteases, regulated by calcium homeostasis, play an important role in regulating the components of the intercalated disk. This review examines the role of calcium in calpain function, how calpains regulate the activity of specific proteins in the intercalated disk, and the molecular mechanisms behind calpain dysregulation and its impact on heart diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Ivan Shapovalov, Danielle Harper, Peter A. Greer
Summary: Pre-clinical and clinical studies have shown that inhibition of calpain-1 and calpain-2 has the potential to attenuate carcinogenesis and block metastasis of aggressive tumors. However, the wide range of substrates and cleavage products, as well as inconsistencies in model systems, highlight the need for a better understanding of physiological substrates and how calpain cleavage affects their functions in cellular processes. The development of isoform specific calpain inhibitors remains an important goal with therapeutic potential in cancer and other diseases.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2022)
Article
Multidisciplinary Sciences
Aditya Naldurtiker, Phaneendra Batchu, Brou Kouakou, Thomas H. Terrill, Arshad Shaik, Govind Kannan
Summary: This study used RNA-seq technology to analyze the transcriptome and meat quality of goats subjected to transportation stress. The results showed that transportation stress increased plasma epinephrine concentrations, decreased muscle glycogen concentrations, and increased calpastatin levels. Additionally, transportation stress affected meat quality characteristics and muscle protein levels.
SCIENTIFIC REPORTS
(2022)