Article
Biochemistry & Molecular Biology
Yung-Te Hou, Chia-Chun Wu, Wen-Ting Wang, Wen-Tse Yang, Ying-Hsiu Liao, Chien-Yu Chen
Summary: In this study, RNA-Seq was used to monitor the maturity and dynamic characteristics of in vitro hepatocyte cultures. The results showed that RNA-Seq can be used to infer the success of in vitro hepatocyte cultures and provide a more comprehensive list of factors related to hepatocyte differentiation. This monitoring system has high potential in medical applications and may also be a novel method for diagnosing liver-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Anirban Roy, Runu Chakraborty
Summary: This study developed a nanoformulation using catechin and polylactide-co-glycolide to improve the treatment of gastric ulcers. The results showed that nanocatechin had better bioavailability and could reduce gastric damage at a significantly lower dose (2.5 mg/kg) by protecting against reactive oxygen species, restoring mitochondrial integrity, and downregulating MMP-9 and other inflammatory mediators.
Article
Multidisciplinary Sciences
Isabella B. Metelmann, Sebastian Kraemer, Matthias Steinert, Stefan Langer, Peggy Stock, Olga Kurow
Summary: This study developed a 3D organotypic model of the pleural mesothelium and demonstrated its similarity to human pleura tissue in terms of morphology and microenvironment. The permeability test of the model indicated its ability to mimic physiological conditions in vivo.
Review
Cell Biology
Ye Xie, Jia Yao, Weilin Jin, Longfei Ren, Xun Li
Summary: The review suggests that hepatocyte-like cells (HLCs), derived from stem cells or modified adult cells, have advantages in cell source and in vitro expansion ability. However, most induced HLCs are still immature and exhibit heterogeneous differentiation, limiting their clinical application. Various methods have been developed to promote the maturation of HLCs for in vivo transplantation and in vitro model establishment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Rishika Agarwal, Tanja Dittmar, Hans-Dietmar Beer, Michael Kunz, Simon Mueller, Elisabeth A. A. Kappos, Emmanuel Contassot, Alexander A. Navarini
Summary: This study presents a method for generating human epidermis organotypic cultures (HEOCs) from primary keratinocytes and an immortalized keratinocyte cell line (KerTr). The HEOCs were characterized and shown to express markers associated with proliferation and differentiation. The ability to generate HEOCs reproducibly on a large scale makes them a valuable model for drug screening and studying epidermal pathologies.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Evgeniya Shcherbinina, Tatiana Abakumova, Daniil Bobrovskiy, Ilia Kurochkin, Ksenia Deinichenko, Elena Stekolshchikova, Nickolay Anikanov, Rustam Ziganshin, Pavel Melnikov, Ekaterina Khrameeva, Maria Logacheva, Timofei Zatsepin, Olga Sergeeva
Summary: Long non-coding RNAs play an important role in glucose and lipid metabolism. The murine lncRNA Falcor/LL35, a functional analog of human DEANR1/linc00261, is involved in the regulation of glycolysis and lipid biosynthesis. The depletion of LL35 affects the Notch and NF-kappa B signaling pathways, leading to decreased proliferation and migration of hepatocytes.
Article
Chemistry, Analytical
Robert Knitsch, Mohammad AlWahsh, Hannes Raschke, Joerg Lambert, Roland Hergenroeder
Summary: Three-dimensional cell cultures are becoming increasingly important in biochemical research, requiring adaptations of established analytical techniques for more accurate representation of tissue features. This study presents an NMR-based approach to obtain dynamically radial metabolite profiles for cell spheroids, one of the most frequently used 3D models, using spatially selective NMR pulse sequences and a commercially available cryogenic NMR probe.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Svetlana Rodimova, Vadim Elagin, Maria Karabut, Irina Koryakina, Alexander Timin, Vladimir Zagainov, Mikhail Zyuzin, Elena Zagaynova, Daria Kuznetsova
Summary: Finding new criteria, testing drugs for hepatotoxicity, and studying the mechanisms of hepatotoxicity at a molecular level are challenging and require adequate research models and tools. Microfluidic chips offer a promising in vitro model, but more complex models are needed for comprehensive information. FLIM is a new label-free approach for studying liver pathology.
Article
Clinical Neurology
Gareth Morris, Massimo Avoli, Christophe Bernard, Kate Connor, Marco de Curtis, Chris G. Dulla, John G. R. Jefferys, Caterina Psarropoulou, Kevin J. Staley, Mark O. Cunningham
Summary: In vitro preparations offer valuable tools for studying and modeling seizures and epilepsy, as well as screening anti-seizure compounds. However, these models are simplifications of reality and have limitations. Therefore, a combination of in vitro models, in vivo studies, chronic epilepsy animal models, and human cell/tissue-derived preparations is recommended to maximize their potential value.
Review
Multidisciplinary Sciences
Maria Jimenez Ramos, Lucia Bandiera, Filippo Menolascina, Jonathan Andrew Fallowfield
Summary: Non-alcoholic fatty liver disease (NAFLD) is a global healthcare challenge, affecting one in four adults, and predicted to have increasing death rates. Non-alcoholic steatohepatitis (NASH), a progressive form of NAFLD, can lead to fibrosis, cirrhosis, and hepatocellular carcinoma. However, there are currently no licensed medical treatments for NAFLD-NASH. The development of clinically relevant in vitro models and the application of emerging technologies offer promising opportunities for understanding NAFLD biology and identifying potential treatments.
Article
Multidisciplinary Sciences
Moke Liu, Luna Liu, Honglin Guo, Xiude Fan, Tianbao Liu, Chao Xu, Zhao He, Yongfeng Song, Ling Gao, Shanshan Shao, Jiajun Zhao, Peng Lu
Summary: In this study, a mouse model carrying the HNF1a P291fsinsC mutation was established, and it was found that this mutation led to liver steatosis. Further analysis revealed changes in innate immune response and lipid metabolism pathways. The study also showed that HNF1a inhibited the expression of CFD in hepatocytes, and that CFD regulated hepatocyte lipid deposition.
Article
Neurosciences
Fatme Seval Ismail, Pedro M. Faustmann, Marie-Luise Kuemmel, Eckart Foerster, Timo Jendrik Faustmann, Franco Corvace
Summary: This study aimed to investigate the effects of the new generation antiseizure medication brivaracetam (BRV) on astrocytes and microglia in an inflammation model. The results showed that BRV can modulate the activation state of microglia under inflammatory conditions, but has limited effects on astroglial connexin 43 (Cx43) expression and gap-junctional coupling.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Nicole Verdile, Federica Camin, Radmila Pavlovic, Rolando Pasquariello, Milda Stuknyte, Ivano De Noni, Tiziana A. L. Brevini, Fulvio Gandolfi
Summary: A culture platform that efficiently promotes cell differentiation into mature enterocytes was developed and compared with three other options. The presence of fibroblasts in the platform was found to be particularly effective in enhancing the differentiation of epithelial cells and closely recreating the complexity of the intestinal mucosa.
Article
Cell Biology
Vanessa Etschmaier, Muammer Uecal, Birgit Lohberger, Markus Absenger-Novak, Dagmar Kolb, Annelie Weinberg, Ute Schaefer
Summary: An ex vivo rat femur slice model was used to investigate the mechanisms of growth impairment following trauma-related bone fractures. The study showed disrupted endochondral ossification, altered expression patterns of specific genes, and increased chondrocyte metabolic activity in the injured area. The presence of stem cells, fibroblasts, and chondrocytes was also observed at the injury site. These findings suggest that the ex vivo organotypic GP injury model is valuable for studying GP regeneration post-trauma and other tissue engineering and disease studies.
Article
Biotechnology & Applied Microbiology
Morten Seirup, Srikumar Sengupta, Scott Swanson, Brian E. McIntosh, Mike Collins, Li-Fang Chu, Zhang Cheng, David U. Gorkin, Bret Duffin, Jennifer M. Bolin, Cara Argus, Ron Stewart, James A. Thomson
Summary: In this study, the molecular changes associated with dedifferentiation of hepatocytes in culture were investigated using lineage tracing and comprehensive profiling. The findings revealed three distinct phases of dedifferentiation and drastic changes in chromatin accessibility during the early stages of culture. Furthermore, several candidate transcription factors potentially involved in the dedifferentiation process were identified through motif analysis of open chromatin regions.