Article
Chemistry, Multidisciplinary
Anqi Yang, Xiang Lin, Zijian Liu, Xin Duan, Yurou Yuan, Jiaxuan Zhang, Qilin Liang, Xianglin Ji, Nannan Sun, Huajun Yu, Weiwei He, Lili Zhu, Bingzhe Xu, Xudong Lin
Summary: A novel in vivo screening strategy combining hierarchically structured biohybrid triboelectric nano-generators (HB-TENGs) arrays with computational bioinformatics analysis for high-throughput pharmacological evaluation using Caenorhabditis elegans is described. The animals' behaviors are efficiently converted into friction deformation through HB-TENGs, and the resulting triboelectric signals are extracted for various bioinformation of the screened compounds. The information-rich electrical readouts are sufficient to predict a drug's identity by multiple-Gaussian-kernels-based machine learning methods.
Article
Immunology
Ruan Fourie, Jacobus Albertyn, Olihile Sebolai, Onele Gcilitshana, Carolina H. Pohl
Summary: The interaction between Candida albicans and Pseudomonas aeruginosa affects early biofilm formation and virulence of C. albicans in an infection model. Deletion of SET3 does not affect C. albicans morphology in the presence of P. aeruginosa, but it reduces virulence in a Caenorhabditis elegans infection model.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Louis Camaioni, Dylan Lambert, Boualem Sendid, Muriel Billamboz, Samir Jawhara
Summary: In this study, a screening of compounds from the JUNIA chemical library led to the discovery of 11 hybrid compounds with pyrrolidinone rings and hydrazine moieties that showed excellent antifungal activities against C. albicans. Three of these compounds (Hyd.H, Hyd.OCH3, and Hyd.Cl) exhibited rapid fungicidal activity and significant antifungal activity against fluconazole- or caspofungin-resistant C. albicans strains. These compounds also showed no cytotoxicity to human cancer cell lines and reduced Candida biofilm formation. In a C. albicans infection model with Caenorhabditis elegans, the hydrazine-based compounds significantly reduced nematode mortality.
Article
Multidisciplinary Sciences
Amanda C. Smith, Judy Dinh, Meleah A. Hickman
Summary: While pathogens like Candida albicans can be deadly, they often cause non-lethal infections as well. Various mini-model systems have been developed to study C. albicans pathogenesis and assess its virulence, offering a cost-effective and efficient alternative to traditional murine models.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Immunology
N. Z. Mokoena, H. Steyn, A. Hugo, T. Dix-Peek, C. Dickens, O. M. N. Gcilitshana, O. Sebolai, J. Albertyn, C. H. Pohl
Summary: The intake of omega-3 polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA), has known health benefits due to its anti-inflammatory effects. This study investigated the potential antifungal properties of EPA in vivo using the Caenorhabditis elegans infection model. EPA supplementation altered the lipid composition of C.elegans and delayed killing by C.albicans through inhibition of hyphal formation. EPA also affected gene expression related to biofilm formation in C.albicans and stimulated the immune response of C.elegans, suggesting a link between EPA and host susceptibility to microbial infection in this model.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Immunology
N. Z. Mokoena, H. Steyn, A. Hugo, T. Dix-Peek, C. Dickens, O. M. N. Gcilitshana, O. Sebolai, J. Albertyn, C. H. Pohl
Summary: The intake of omega-3 polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA), has anti-inflammatory and antifungal properties. In this study using the Caenorhabditis elegans infection model, it was found that EPA supplementation changed the lipid composition of the nematodes and delayed the killing of Candida albicans. This was achieved by inhibiting hyphal formation and stimulating the immune response, suggesting a link between EPA and host susceptibility to microbial infection.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Brandon M. Murareanu, Noelle Antao, Winnie Zhao, Aurore Dubuffet, Hicham El Alaoui, Jessica Knox, Damian C. Ekiert, Gira Bhabha, Peter J. Roy, Aaron W. Reinke
Summary: In this study, a high-throughput screening method was developed to identify chemical inhibitors of microsporidia infection. Through experiments on Caenorhabditis elegans, 11 potential microsporidia inhibitors were identified, among which five compounds prevent microsporidia infection by inhibiting spore firing.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Jie Tu, Tianbao Zhu, Qingwen Wang, Wanzhen Yang, Yahui Huang, Defeng Xu, Na Liu, Chunquan Sheng
Summary: Candida auris is a serious public health threat that has caused a global epidemic due to its multi-drug resistance, remarkable transmissibility, and high mortality. A novel benzoanilide antifungal agent, compound A1, was discovered through a comprehensive strategy involving phenotypic screening, hit optimization, antifungal assays, and mechanism exploration. Compound A1 showed potent efficacy against Candida auris infection both in vitro and in vivo. Mechanistic investigation revealed that compound A1 inhibited the biosynthesis of virulence factors and fungal cell walls by blocking glycosylphosphatidylinositol (GPI) and GPI-anchored proteins. Therefore, compound A1 represents a promising lead compound for combating drug-resistant candidiasis.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Infectious Diseases
Louis Camaioni, Bastien Ustyanowski, Mathys Buisine, Dylan Lambert, Boualem Sendid, Muriel Billamboz, Samir Jawhara
Summary: Candida albicans is a pathogenic yeast with increasing resistance to antifungal drugs. This study identified hinokitiol, a natural compound extracted from cypress wood, as showing excellent antifungal activity against C. albicans. Hi not only inhibited candida growth and biofilm formation, but also displayed anti-inflammatory effects against lipopolysaccharide-induced cytokine expression in macrophages.
Article
Immunology
Amanda C. Smith, Levi T. Morran, Meleah A. Hickman
Summary: The ability to generate genetic variation facilitates rapid adaptation in Candida albicans. Innate immune components in the host, including antimicrobial peptides and reactive oxygen species, induce host-associated genomic changes in C. albicans, promoting rapid evolution.
INFECTION AND IMMUNITY
(2022)
Article
Infectious Diseases
Bonang M. Mochochoko, Obinna T. Ezeokoli, Olihile Sebolai, Jacobus Albertyn, Carolina H. Pohl
Summary: Components of the iron reductive pathway of Candida albicans play crucial roles in biofilm formation, prostaglandin E-2 (PGE(2)) production, and virulence. Deletion of specific genes can lead to alterations in these biological functions.
Article
Pharmacology & Pharmacy
Huijuan Yao, Ajing Xu, Jingxian Liu, Fang Wang, Huimin Yao, Jihui Chen
Summary: This study aimed to evaluate the in vivo antimicrobial activity of specific drugs against Carbapenem-resistant Klebsiella pneumoniae (CRKP) using a Caenorhabditis elegans (C. elegans) model system. The results showed that the C. elegans model system was consistent with the conventional methods and demonstrated potential advantages in a clinical setting.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Patricia K. Dranchak, Erin Oliphant, Bryan Queme, Laurence Lamy, Yuhong Wang, Ruili Huang, Menghang Xia, Dingyin Tao, James Inglese
Summary: This article introduces a method of high-throughput screening using C. elegans, where phenotypic changes in GFP-expressing organisms guide subsequent analyses. This approach will facilitate the analysis of C. elegans orthologous phenotypes of human pathologies and enable drug library profiling for a range of therapeutic indications.
DISEASE MODELS & MECHANISMS
(2023)
Article
Environmental Sciences
Jiaying Wu, Yue Gao, Jing Xi, Xinyue You, Xiaohong Zhang, Xinyu Zhang, Yiyi Cao, Peichuan Liu, Xiang Chen, Yang Luan
Summary: In this study, an automated and highly-integrated platform was developed for high-throughput and in situ toxicity testing using Caenorhabditis elegans. The platform combines various modules and devices to achieve efficient worm dispensing, long-term maintenance, toxicity monitoring, imaging, and behavioral analysis. It also enables remote operation and phenotype calculation through connection to the web.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Microbiology
Nicole Robbins, Leah E. Cowen
Summary: Fungi have a significant impact on human health, causing numerous infections and deaths worldwide each year. The emergence of drug-resistant fungal pathogens and the lack of effective treatment options exacerbate the public health burden. However, scientific advances in chemistry, genetics, and biochemistry methodologies have enabled the discovery of novel antifungal compounds and exploration of potential therapeutic approaches.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Article
Emergency Medicine
Adam C. Levine, Pranav Prathap Shetty, Ryan Burbach, Sambhavi Cheemalapati, Justin Glavis-Bloom, Tess Wiskel, J. Kota T. Kesselly
ANNALS OF EMERGENCY MEDICINE
(2015)
Article
Nutrition & Dietetics
Payal Modi, Sabiha Nasrin, Meagan Hawes, Justin Glavis-Bloom, Nur H. Alam, M. Iqbal Hossain, Adam C. Levine
JOURNAL OF NUTRITION
(2015)
Review
Dermatology
Justin Glavis-Bloom, Scott Vasher, Meghan Marmor, Antonella B. Fine, Philip A. Chan, Karen T. Tashima, John R. Lonks, Erna M. Kojic
Article
Immunology
Michael A. Smit, Ian C. Michelow, Justin Glavis-Bloom, Vanessa Wolfman, Adam C. Levine
CLINICAL INFECTIOUS DISEASES
(2017)
Article
Medicine, Research & Experimental
Adam C. Levine, Carrie Teicher, Adam R. Aluisio, Tess Wiskel, Pola Valles, Miguel Trelles, Justin Glavis-Bloom, Rebecca F. Grais
Article
Public, Environmental & Occupational Health
Adam C. Levine, Justin Glavis-Bloom, Payal Modi, Sabiha Nasrin, Bita Atika, Soham Rege, Sarah Robertson, Christopher H. Schmid, Nur H. Alam
LANCET GLOBAL HEALTH
(2016)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Justin Glavis-Bloom, Ruth Rizzo, Amit Sura
JOURNAL OF THE AMERICAN COLLEGE OF RADIOLOGY
(2017)
Article
Multidisciplinary Sciences
Payal Modi, Justin Glavis-Bloom, Sabiha Nasrin, Allysia Guy, Erika P. Chowa, Nathan Dvor, Daniel A. Dworkis, Michael Oh, David M. Silvestri, Stephen Strasberg, Soham Rege, Vicki E. Noble, Nur H. Alam, Adam C. Levine
Article
Computer Science, Information Systems
Michelle Bardis, Roozbeh Houshyar, Chanon Chantaduly, Alexander Ushinsky, Justin Glavis-Bloom, Madeleine Shaver, Daniel Chow, Edward Uchio, Peter Chang
Article
Multidisciplinary Sciences
Daniel S. Chow, Justin Glavis-Bloom, Jennifer E. Soun, Brent Weinberg, Theresa Berens Loveless, Xiaohui Xie, Simukayi Mutasa, Edwin Monuki, Jung In Park, Daniela Bota, Jie Wu, Leslie Thompson, Bernadette Boden-Albala, Saahir Khan, Alpesh N. Amin, Peter D. Chang
Article
Radiology, Nuclear Medicine & Medical Imaging
Sriram Rao, Justin Glavis-Bloom, David Kakish, Karen Tran-Harding, Daniel S. Chow, Michael Nguyentat, Eric O. Yeates, Jeffry Nahmias, Roozbeh Houshyar
Summary: The COVID-19 pandemic has caused disruptions in healthcare staffing and operations. This study found that after the easing of stay-at-home orders, there was an increase in trauma volumes and the number of trauma computed tomography (CT) exams, but with longer turnaround times.
EMERGENCY RADIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
John Mongan, Marc D. Kohli, Roozbeh Houshyar, Peter D. Chang, Justin Glavis-Bloom, Andrew G. Taylor
Summary: This study developed an algorithm that can accurately detect IVC filters on radiographs without human assistance, which can be used to screen for patients needing IVC filter retrieval.
ABDOMINAL RADIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Justin Glavis-Bloom, Unikora Yang, Daniel Nahl, Fariba Goodarzian, Amit Sura
CURRENT PROBLEMS IN DIAGNOSTIC RADIOLOGY
(2020)
Article
Public, Environmental & Occupational Health
Adam C. Levine, Justin Glavis-Bloom, Payal Modi, Sabiha Nasrin, Soham Rege, Chieh Chu, Christopher H. Schmid, Nur H. Alam
GLOBAL HEALTH-SCIENCE AND PRACTICE
(2015)
Article
Public, Environmental & Occupational Health
Catherine Mugeni, Adam C. Levine, Richard M. Munyaneza, Epiphanie Mulindahabi, Hannah C. Cockrell, Justin Glavis-Bloom, Cameron T. Nutt, Claire M. Wagner, Erick Gaju, Alphonse Rukundo, Jean Pierre Habimana, Corine Karema, Fidele Ngabo, Agnes Binagwaho
GLOBAL HEALTH-SCIENCE AND PRACTICE
(2014)