Article
Engineering, Electrical & Electronic
Taiji Dong, Bingkun Gao, Bing Yan, Yekun Zhou, Yunkai Wang, Weicheng Wang, Kaichuan Xu, Chunlei Jiang, Zengbo Wang
Summary: In the field of biomedicine, the development of a tapered fiber optic biosensor (TFOB) allows for nondestructive and label-free detection of individual living cells. Through theoretical and simulation analysis, the fiber tip of the TFOB is found to be a highly sensitive sensing element that translates the intensity change of the evanescent field into changes in output power. Experimental results demonstrate the TFOB's ability to detect low-amplitude vibration signals and measure respiratory vibrations of pasta yeast cells.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Antony Vincy, Yohan Gaikwad, Harshita Agarwal, Neha Jain, Raviraj Vankayala
Summary: In this study, a paper-based colorimetric dipstick sensor based on the principle of Prussian blue synthesis was developed for highly sensitive and rapid detection of bacterial contamination. The sensor is easy to fabricate and can detect bacteria up to 10(1) CFU/mL within minutes.
Article
Multidisciplinary Sciences
Jan Oscar Cross-Zamirski, Elizabeth Mouchet, Guy Williams, Carola-Bibiane Schonlieb, Riku Turkki, Yinhai Wang
Summary: Cell Painting is a detection method used in drug discovery to predict bioactivity, assess toxicity, and understand the mechanisms of chemical and genetic disruptions. This study investigates label-free Cell Painting and finds that it can predict five fluorescent channels from brightfield input. Deep learning models were trained and validated, achieving high correlation and accuracy in predicting image features. The label-free Cell Painting method also shows potential for toxicity analysis and repurposing imaging channels for targeted biological research.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Tommaso Vannocci, Luca Quaroni, Antonio de Riso, Giulia Milordini, Magda Wolna, Gianfelice Cinque, Annalisa Pastore
Summary: In this study, infrared microscopy was used to monitor the metabolic turnover of individual mammalian cells in real time, revealing major biochemical differences between adherent cells and suspended cells. Spectroscopic variations and spectral oscillations in the infrared domain were observed when using synchrotron infrared radiation, suggesting a potential application as a reporter of cellular conditions. These findings establish IR microscopy as a valuable label-free method for studying real-time metabolism in cells with distinct morphological states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Ke Shi, Na Na, Jin Ouyang
Summary: We developed a label- and enzyme-free single molecule fluorescence counting strategy for detecting HIV DNA fragments. This method showed high sensitivity and selectivity, making it a promising tool for HIV DNA analysis in clinical applications.
Article
Multidisciplinary Sciences
Shuyao Gu, Rachel M. Lee, Zackery Benson, Chenyi Ling, Michele Vitolo, Stuart S. Martin, Joe Chalfoun, Wolfgang Losert
Summary: Collective cell migration, a crucial factor in biological functions such as cancer metastasis, involves diverse cell behaviors. Researchers have developed an AI-based pipeline to segment and track cell nuclei, enabling quantification and analysis of collective motion. This approach, which does not require additional training data, has the potential to provide new insights into existing libraries of collective motion images and may serve as an indicator of metastatic potential.
Article
Fisheries
Javier Mayor, Alberto Cuesta, Cristobal Espinosa-Ruiz, M. Angeles Esteban
Summary: This study investigated the effects of Astaxanthin (AX) on various fish pathogenic bacteria and fish cell lines. The results showed that AX had different effects on different bacteria and cell lines, providing valuable insights into the mechanism of action of AX in aquaculture.
AQUACULTURE REPORTS
(2023)
Article
Biochemical Research Methods
Shivanshu Mishra, Pharyanshu Kachhawa, Amber Kumar Jain, Rajiv Ranjan Thakur, Nidhi Chaturvedi
Summary: This work demonstrates the rapid, label-free, and specific detection of the HER2 antigen using a gallium nitride high electron mobility transistor (GaN-HEMT). The sensor is functionalized with a thiol-based chemistry to immobilize the HER2 antibody. Experimental results confirm the successful functionalization and detection of the HER2 antigen in a broad concentration range. The study also investigates the long-term viability and reusability of the sensor. A three-binding-site model for HER2 detection using GaN-HEMT is developed for the first time.
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Robert Strutt, Felix Sheffield, Nathan E. Barlow, Anthony J. Flemming, John D. Harling, Robert Law, Nicholas J. Brooks, Laura M. C. Barter, Oscar Ces
Summary: We report a new technology platform, UV-DIB, that allows label-free measurement of small molecule translocation rates. By coupling droplet interface bilayer technology with fiber optics for ultraviolet spectroscopy analysis, we are able to study diffusion behavior at lipid interfaces. Our system quantifies passive diffusion rates and provides precise results by investigating the effects of lipid composition and permeant structure on permeability.
Article
Chemistry, Analytical
Junhyun Park, Hyogu Han, Chihyun Park, Jun Ki Ahn
Summary: In this study, a personal glucose pyrophosphatase (PPase) detection method is demonstrated using cascade enzymatic reaction (CER) promoted by hexokinase and pyruvate kinase. Compared with previous methods, this method eliminates the need for expensive equipment and complex procedures, and allows quantitative evaluation of PPase activity within a dynamic linear range.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Maria Carcelen, Veronica Vidal, Alfredo Franco, Marcos Gomez, Fernando Moreno, Jose L. Fernandez-Luna
Summary: This article introduces a simple, fast and label-free method based on a gold nanohole array biosensor to detect the spectral response and changes in cell-matrix adhesion of cancer cells. The experimental results show that this biosensor can effectively distinguish different cell populations in the cell cycle and detect changes in cell-matrix interaction in real time.
Article
Immunology
Seong-Eun Kim, Suji Yun, Junsang Doh
Summary: This study found that the extracellular matrix on the surface of solid tumor cells affects the cytotoxicity of immune cells. Solid tumor cells with high adhesion are more resistant to immune cell-mediated killing, while solid tumor cells with low adhesion are more easily killed by immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Aleksandr Egunov, Zehua Dou, Dmitriy D. Karnaushenko, Franziska Hebenstreit, Nicole Kretschmann, Katja Akgun, Tjalf Ziemssen, Daniil Karnaushenko, Mariana Medina-Sanchez, Oliver G. Schmidt
Summary: An innovative rolled-up impedimetric microfluidic sensor, named sensor-in-a-tube, is introduced for simultaneous analysis of single human monocytes CD14+ and their extracellular medium during lipopolysaccharide (LPS)-induced activation. The platform utilizes electrical impedance spectroscopy to detect the correspondence between cell activation stages and the electrical properties of the cell surrounding medium without the need for surface functionalization or labeling. The sensor-in-a-tube platform is demonstrated as a sensitive and reliable tool for precise single cell analysis towards immune-deficient diseases diagnosis.
