Article
Immunology
Marco Antonio Cabrera Gonzalez, Ana Alice Maia Goncalves, Jennifer Ottino, Jaqueline Costa Leite, Lucilene Aparecida Resende, Otoni Alves Melo-Junior, Patricia Silveira, Mariana Santos Cardoso, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno, Renato Lima Santos, Tatiane Furtado de Carvalho, Giani Martins Garcia, Paulo Ricardo de Oliveira Paes, Alexsandro Sobreira Galdino, Miguel Angel Chavez-Fumagalli, Marilia Martins Melo, Denise Silveira-Lemos, Olindo Assis Martins-Filho, Walderez Ornelas Dutra, Vanessa Carla Furtado Mosqueira, Rodolfo Cordeiro Giunchetti
Summary: Visceral leishmaniasis (VL) is a fatal disease caused by Leishmania infantum, and dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) is important in controlling human and CVL infections by reducing L. infantum infection. This study evaluated the potential of two polymeric nanoformulations loaded with Leishmania amazonensis antigens as a VL vaccine using golden hamsters as a model. The results showed that the vaccines had no adverse reactions and significantly reduced the parasitic load in the liver and spleen. These vaccines may be promising candidates for a VL vaccine.
Article
Microbiology
Derya Topuz Ata, Muattaz Hussain, Michael Jones, Jonathan Best, Martin Wiese, Katharine Christine Carter
Summary: In this study, transgenic Leishmania tarentolae expressing ?GCS from three pathogenic species were used to evaluate their protective effect against infection. The live vaccine showed a significant reduction in parasite burdens compared to controls, suggesting that ?GCS could be a candidate vaccine for leishmaniasis.
Review
Immunology
Thalia Pacheco-Fernandez, Greta Volpedo, Sreenivas Gannavaram, Parna Bhattacharya, Ranadhir Dey, Abhay Satoskar, Greg Matlashewski, Hira L. Nakhasi
Summary: Leishmaniasis is a disease caused by parasitic protozoa transmitted by infected sandflies, affecting over 1 billion people worldwide. The successful vaccination procedure known as leishmanization, which has been used in the Middle East for decades, is no longer practiced due to safety and ethical concerns. New genome editing technologies may allow for the development of a second generation leishmanization that could enter human trials.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Rupal Ojha, Kailash Chand, Balachandar Vellingiri, Vijay Kumar Prajapati
Summary: This study aimed to develop a more potent vaccine candidate against visceral leishmaniasis. The recombinant vaccine candidate Leisp was expressed, purified, and characterized. The expression modulation of different cytokines was evaluated in THP1 cell lines, and the impact of the vaccine protein candidates in infected conditions was determined. The results showed that the candidate vaccine could significantly upregulate the expression of pro-inflammatory cytokines, indicating its potential inductive efficacy for inflammation in Leishmania-infected cells.
Article
Immunology
Sabrina de Oliveira Emerick, Thais Vieira de Carvalho, Bianca Meirelles Miranda, Adriana Carneiro da Silva, Thais Viana Fialho Martins, Leandro Licursi de Oliveira, Eduardo de Almeida Marques-da-Silva
Summary: The study evaluated the immunization using different vaccines and adjuvants against L. chagasi infection, and found that the LPG3-SAP vaccine significantly reduced parasite load in both spleen and liver in mice, inducing Th1/Th17 response and IL-10 production.
Article
Microbiology
Catherine W. Cai, Anne O'Shea, Christopher S. S. Eickhoff, Hongjie Guo, Warren G. G. Lewis, Stephen M. M. Beverley, Daniel F. F. Hoft
Summary: This study demonstrated the successful generation of recombinant Leishmania major parasites expressing the immunogenic T. cruzi trans-sialidase (TS) antigen, which induced T cell and antibody responses in mice and provided protective immunity against T. cruzi infection. The findings suggest the potential of using attenuated Leishmania parasites as a vaccine for Chagas disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Joao Guilherme Lino da Silva, Ana Alice Maia Goncalves, Liliam Teixeira Oliveira, Giani Martins Garcia, Mauricio Azevedo Batista, Ludmila Zanandreis de Mendonca, Kelvinson Fernandes Viana, Rita de Cassia Oliveira Sant'Ana, Otoni Alves de Oliveira Melo Junior, Denise Silveira-Lemos, Walderez Ornelas Dutra, Olindo Assis Martins-Filho, Alexsandro Sobreira Galdino, Sandra Aparecida Lima de Moura, Vanessa Carla Furtado Mosqueira, Rodolfo Cordeiro Giunchetti
Summary: Recent studies suggest that antigens in microparticles can enhance the immunogenicity of antiLeishmania vaccines. This study investigates the in situ effect of chitosan-coated poly(D,L-lactic) acid submicrometric particles (SMP) as adjuvants and analyzes their inflammatory profile and toxicity. The results show that SMPs promote cell recruitment without tissue damage and stimulate early innate immune responses. They also demonstrate reduced NK cell count and impaired T and B cell subsets in mice vaccinated with SMPs encapsulating Leishmania braziliensis antigen.
Article
Immunology
Rohit Sharma, Francys Avendano Rangel, Joao Luis Reis-Cunha, Larissa Pinheiro Marques, Claudio P. Figueira, Pedro B. Borba, Sayonara M. Viana, Tom Beneke, Daniella C. Bartholomeu, Camila I. de Oliveira
Summary: Studies have shown that centrin-deficient L. braziliensis mutant strain LbCen(-/-) exhibits growth arrest at the promastigote stage and impaired survival following macrophage infection. In animal models, LbCen(-/-) fails to induce lesion formation and significantly reduces parasite burden. Centrin-deficient Leishmania may serve as potential candidates for vaccine development against American Tegumentary Leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Deep Bhowmik, Achyut Bhuyan, Seshan Gunalan, Gugan Kothandan, Diwakar Kumar
Summary: In this study, a global multiepitope subunit vaccination against visceral leishmaniasis (VL) was designed using innovative immunoinformatics technique. The vaccine showed promising immunogenicity and has the potential to eradicate the disease in the future.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Sara Aiman, Abbas Ahmad, Azmat Ali Khan, Amer M. Alanazi, Abdus Samad, Syed Luqman Ali, Chunhua Li, Zhiguang Ren, Asifullah Khan, Saadullah Khattak
Summary: In this study, potential vaccine candidate proteins of L. tropica were prioritized using proteomic and vaccinomic approaches. Multi-epitope constructs were designed based on B- and T-cell epitope prediction, and immunogenic adjuvant sequences were incorporated to enhance immunological potential. Molecular docking and simulation analysis revealed a vaccine design with stable interactions with TLR4.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Paul M. Kaye, Greg Matlashewski, Sakshi Mohan, Epke Le Rutte, Dinesh Mondal, Ali Khamesipour, Stefano Malvolti
Summary: Leishmania infections are a global concern, affecting numerous countries and putting 600 million to 1 billion people at risk. Visceral leishmaniasis causes almost 20,000 deaths each year, with children under 5 at the highest risk. Among neglected tropical diseases, leishmaniasis is one of the most significant in terms of mortality and morbidity. With the increasing burden of disease, climate change, urbanization, and drug resistance, the development of leishmaniasis vaccines is imperative.
Article
Multidisciplinary Sciences
Helen Ashwin, Jovana Sadlova, Barbora Vojtkova, Tomas Becvar, Patrick Lypaczewski, Eli Schwartz, Elizabeth Greensted, Katrien Van Bocxlaer, Marion Pasin, Kai S. Lipinski, Vivak Parkash, Greg Matlashewski, Alison M. Layton, Charles J. Lacey, Charles L. Jaffe, Petr Volf, Paul M. Kaye
Summary: Leishmaniasis is considered a vaccine-preventable disease, but costs and uncertainty hinder progress in vaccine development. Controlled human infection models offer a way to speed up vaccine development and gain better understanding of disease pathogenesis.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Mahmoud Nateghi Rostami, Ali Khamesipour
Summary: Leishmaniasis is a vector-borne neglected tropical disease endemic in over 100 countries worldwide, posing a threat to human health. Lack of a comprehensive biomarker as a surrogate marker of protection or exacerbation remains a barrier for vaccine development. Most understanding of the role of different markers of immune response in the disease has been collected from experimental animal models.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2021)
Correction
Multidisciplinary Sciences
Han Boon Ong, Simon Clare, Adam Jonathan Roberts, Mary Edythe Wilson, Gavin James Wright
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Saeedeh Ebrahimi, Mohsen Kalantari, Hamzeh Alipour, Kourosh Azizi, Qasem Asgari, Mohammad Saleh Bahreini
Summary: Cutaneous leishmaniasis is a neglected tropical disease caused by intracellular parasites, and the current treatment approaches are limited by complications. Therefore, finding new medicines to treat leishmaniasis is essential.
MICROBIAL PATHOGENESIS
(2021)
Article
Pharmacology & Pharmacy
Jafar Mosafer, Amir-Hossein Sabbaghi, Ali Badiee, Solmaz Dehghan, Mohsen Tafaghodi
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Review
Cell Biology
Anis Askarizadeh, Alexandra E. Butler, Ali Badiee, Amirhossein Sahebkar
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Saeedeh Rastakhiz, Mona Yazdani, Sheida Shariat, Atefeh Arab, Amir Abbas Momtazi-Borojeni, Nastaran Barati, Mercedeh Mansourian, Mohamdreza Amin, Azam Abbasi, Zahra Saberi, Seyed Amir Jalali, Ali Badiee, Mahmoud Reza Jaafari
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Davood Mansury, Kiarash Ghazvini, Saeid Amel Jamehdar, Ali Badiee, Mohsen Tafaghodi, Amin Reza Nikpoor, Yousef Amini, Mahmoud Reza Jaafari
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Niloofar Farzad, Nastaran Barati, Amir Abbas Momtazi-Borojeni, Mona Yazdani, Atefeh Arab, Atefeh Razazan, Sheida Shariat, Mercedeh Mansourian, Azam Abbasi, Zahra Saberi, Ali Badiee, Seyed Amir Jalali, Mahmoud Reza Jaafari
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Amir Abbas Momtazi-Borojeni, Mahmoud Reza Jaafari, Ali Badiee, Amirhossein Sahebkar
Article
Chemistry, Multidisciplinary
Parvin Zamani, Jamshid Gholizadeh Navashenaq, Amin Reza Nikpoor, Mahdi Hatamipour, Reza Kazemi Oskuee, Ali Badiee, Mahmoud Reza Jaafari
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Parasitology
Zahra Kavian, Seyedeh Hoda Alavizadeh, Shiva Golmohamadzadeh, Ali Badiee, Ali Khamesipour, Mahmoud Reza Jaafari
Article
Pharmacology & Pharmacy
Amin Reza Nikpoor, Mahmoud Reza Jaafari, Parvin Zamani, Manouchehr Teymouri, Hamed Gouklani, Ehsan Saburi, Shahrzad Amiri Darban, Ali Badiee, Ali Bahramifar, Mahdi Fasihi-Ramandi, Ramezan Ali Taheri
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Ehsan Kaffash, Ali Badiee, Abbas Akhgari, Negar Akhavan Rezayat, Mohammadreza Abbaspour, Farinaz Saremnejad
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Meeting Abstract
Immunology
S. Nikoofal-Sahlabadi, Matbou M. Riahi, K. Sadri, A. Badiee, A. R. Nikpoor, M. R. Jaafari
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Meeting Abstract
Immunology
A. R. Nikpoor, M. R. Jaafari, P. Zamani, M. Teymouri, H. Gouklani, E. Saburi, Amiri S. Darban, A. Badiee, A. Bahramifar, M. Fasihi-Ramandi, R. A. Taheri
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Davood Mansury, Kiarash Ghazvini, Saeid Amel Jamehdar, Ali Badiee, Mohsen Tafaghodi, Amin Reza Nikpooe, Yousef Amini, Mahmoud Reza Jaafari
REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2019)
Article
Medicine, Research & Experimental
Sara Shokooh Saremi, Maryam Shahryari, Razieh Ghoorchian, Hamed Eshaghian, Seyed Amir Jalali, Amin Reza Nikpoor, Mahmoud Reza Jafari, Ali Badiee
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2018)
Article
Parasitology
Mansure Hojatizade, Ali Badiee, Ali Khamesipour, Mahmoud Reza Jaafari
IRANIAN JOURNAL OF PARASITOLOGY
(2019)