Article
Multidisciplinary Sciences
Diego Valenzuela-Miranda, Ana Teresa Goncalves, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Ivan Liachko, Bradley Nelson, Cristian Gallardo-Escarate
Summary: The sea louse Caligus rogercresseyi has become a major obstacle to the sustainable development of salmon aquaculture in Chile. Recent genome sequencing revealed a large bacterial community associated with the sea louse, raising concerns about potential new sanitary threats. By using chromosome proximity ligation (Hi-C) and long-read sequencing, the researchers successfully reconstructed the genomic profiles of the C. rogercresseyi microbiota. This study identified several pathogenic bacteria, virulence factors, and antibiotic resistance genes, while also suggesting a mutualistic relationship between C. rogercresseyi and its associated microbiota.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Melanie Andrews, Tor Einar Horsberg
Summary: This study investigated the effects of thermal treatment on the copepodid and pre-adult II stages of seven populations of L. salmonis. The results showed that while all stages of the parasite had an immediate reaction to thermal treatment, the majority of them recovered over time. Variations in baseline sensitivity were observed between populations, emphasizing the importance of routine in vitro bioassays to determine treatment efficacy.
Article
Fisheries
Tom Mc Dermott, Jack D'Arcy, Suzanne Kelly, Jamie K. Downes, Bogna Griffin, Robert F. Kerr, Damien O'Keeffe, Micheal O'Ceallachain, Louise Lenighan, Felix Scholz, Neil M. Ruane
Summary: Salmon infestations by the salmon louse and amoebic gill disease are major concerns in the Atlantic salmon aquaculture industry in Ireland. Researchers have found that using nano-filtered hyposaline water has proved to be an effective non-medicinal method to reduce parasite levels.
AQUACULTURE REPORTS
(2021)
Article
Biology
Knut Wiik Vollset, Robert J. Lennox, Helge Skoglund, Orjan Karlsen, Eirik Straume Normann, Tore Wiers, Elisabeth Stoger, Bjorn T. Barlaup
Summary: Parasite spillback from domestic animals can disrupt the balance and harm wild populations. Aquaculture-induced parasite spillback on wild salmon has been contentious. A 19-year study on Atlantic salmon demonstrated that the impact of parasite treatment on marine survival decreased over time, the relationship between salmon lice levels and treatment efficacy was weak, and both treated and untreated salmon had lower return rates with higher salmon lice levels. The study suggests that the impact of salmon lice on wild salmon populations should be better considered when evaluating the effects of aquaculture.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Veterinary Sciences
Manuel Lepe-Lopez, Joaquin Escobar-Dodero, Daniel Rubio, Julio Alvarez, Natalia Zimin-Veselkoff, Fernando O. Mardones
Summary: This study utilized the multivariate spatial scan statistic to identify geographic areas and times of sea lice infestation in farmed salmonids in Chile. The results revealed spatial clusters with different infestation levels and associated factors, highlighting the importance of Integrated Pest Management (IPM). Recommendations for an IPM plan were provided, considering factors such as stock density thresholds and chemical control efficacy to prevent resistance and minimize environmental residues.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Fisheries
C. C. Pert, I. S. Wallace, P. MacDonald, S. C. Ives, A. G. Murray, B. Rabe
Summary: This study describes the development and application of towed sentinel cages (TSCs) to assess infestation pressure along trajectories in a Scottish sea loch. The sea lice infestation rates found from TSCs were comparable to those on fixed sentinel cages. Therefore, TSCs could be used to improve the assessment of exposure risk along wild salmonid smolt migration trajectories.
DISEASES OF AQUATIC ORGANISMS
(2023)
Article
Fisheries
Ian Bricknell, Michael Pietrak
Summary: This study investigates the environment of the digestive tract in fed and starved sea lice, with a focus on digestion and osmoregulation. The findings show that starved sea lice have a digestive tract similar in osmolarity to seawater. Even in lice with blood in their digestive system, the haemoglobin level is surprisingly low. Lice that have been feeding on salmon have a higher osmolarity in their digestive tract compared to starved animals, indicating a higher food content. The study confirms that sea lice are osmoconformers, with the haemolymph closely following the osmolarity of the environment.
Article
Fisheries
Jaewoon Jeong, Gregor F. McEwan, Gabriel Arriagada, Cristian Gallardo-Escarate, Crawford W. Revie
Summary: The salmon louse C. rogercresseyi is a common ectoparasite of farmed salmonids in Chile, and further research is required to adequately model its complete population dynamics and support effective control and mitigation on Chilean salmon farms.
JOURNAL OF FISH DISEASES
(2022)
Article
Engineering, Marine
Marta Sole, Marc Lenoir, Jose-Manuel Fortuno, Steffen De Vreese, Mike van der Schaar, Michel Andre
Summary: This study provides the first ultrastructural images of pathological changes in the sensory setae of the first antenna and inner tissues in different stages of Lepeophtheirus salmonis development after sound exposure. The response to sounds and associated injuries in the lice sensory organs could potentially lead to the development of a bioacoustics method for preventing lice infection and treating affected salmons.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2021)
Article
Fisheries
V. Fernandez-Gonzalez, E. M. Ulvan, P. Sanchez-Jerez, O. H. Diserud, K. Toledo-Guedes, N. Casado-Coy, P. Klebert, I. Uglem
Summary: This study aims to evaluate and determine a standardized method to measure sea lice abundance in the salmon farming industry, providing a sampling and analysis strategy for quantifying sea lice larvae. Quantifying sea lice larvae can lead to more accurate predictions of their dispersal and the risk of infestation in salmon farms.
Article
Multidisciplinary Sciences
Sean C. Godwin, Andrew W. Bateman, Anna Kuparinen, Rick Johnson, John Powell, Kelly Speck, Jeffrey A. Hutchings
Summary: Research conducted in the Broughton Archipelago, Canada, reveals a significant decrease in the sensitivity of Pacific salmon lice to emamectin benzoate (EMB) since 2010, resulting in reduced efficacy of EMB treatments. These findings, which were obtained through Indigenous-led agreements and not publicly reported by industry or regulators, suggest that controlling salmon lice outbreaks in Pacific farms will be more challenging in the future.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Jeong Jaewoon, Arriagada Gabriel, Crawford W. Revie
Summary: This study explores the impact of target setting on the reporting of sea louse infestation on salmon farms. The findings suggest that the accuracy of reported data can be influenced by the specification of threshold levels. Researchers and fish health professionals should be aware of potential biases in these data, and regulators should carefully consider the unintended consequences of setting certain threshold levels.
Article
Fisheries
Carter R. VanIderstine, Jordan D. Poley, Shona K. Whyte, Sara L. Purcell, Mark D. Fast
Summary: The study investigated the effects of H2O2 exposure on survival and antioxidant-related gene expression in salmon lice. Results showed significant upregulation of seven genes in males and one gene in females after exposure. Temperature and pretreatment with EMB did not impact survival after exposure to H2O2.
JOURNAL OF FISH DISEASES
(2021)
Editorial Material
Fisheries
Ingrid A. Johnsen, Alison Harvey, Pal Naeverlid Saevik, Anne D. Sandvik, Ola Ugedal, Bjorn Adlandsvik, Vidar Wennevik, Kevin A. Glover, Orjan Karlsen
Summary: A model system was developed to estimate salmon lice-induced mortality, calibrated against empirical observations. Critics claim the model overestimates mortality, but their analysis may have shortcomings. Discrepancy between modelled and observed data does not necessarily indicate flaws, but can provide valuable supplementary information.
ICES JOURNAL OF MARINE SCIENCE
(2021)
Article
Biology
Hulda Maria Hardardottir, Rune Male, Frank Nilsen, Sussie Dalvin
Summary: Chitin synthase is a crucial enzyme involved in the survival and reproduction of aquatic organisms, with different expressions and functions in different tissues. Through RNA interference experiments, it was found that the chitin synthases of L. salmonis have significant impacts on the external cuticle and internal organs of lice at different developmental stages.
Article
Fisheries
Janet Velazquez, Alianet Rodriguez, Hasel Aragon, Arlette Haidar, Marcos Gonzalez, Rodolfo Valdes, Hilda Elsa Garay, David Diago Abreu, Yassel Ramos, Ania Cabrales, Antonio Morales, Osmany Gonzalez, Fidel Herrera, Mario Pablo Estrada, Yamila Carpio
Summary: In this study, a monoclonal antibody against tilapia IgM heavy chain was successfully produced and characterized using a peptide-based strategy. The antibody showed specificity and efficacy in studying the humoral immune response in fish species, demonstrating its utility as a molecular tool for fish immunology research.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pilar Mur, Lorena Magraner-Pardo, Sandra Garcia-Mulero, Anna Diez-Villanueva, Jesus del Valle, Elsa Ezquerro, Conxi Lazaro, Gabriel Capella, Victor Moreno, Rebeca Sanz-Pamplona, Tirso Pons, Laura Valle
Summary: Germline variants affecting the proofreading activity of POLE and POLD1 can predispose to colorectal adenomas and carcinomas. Disruptive variants and missense variants outside the exonuclease domain (ED) of these polymerases generally do not predispose to cancer, but specific variants may indirectly affect proofreading activity. The variant POLD1 c.883G>A; p.(Val295Met) located upstream of the ED does not alter proofreading activity or associate with cancer.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Fisheries
Alianet Rodriguez, Janet Velazquez, Luis Gonzalez, Tania Rodriguez-Ramos, Brian Dixon, Fidel Herrera Miyares, Antonio Morales, Osmany Gonzalez, Mario Pablo Estrada, Yamila Carpio
Summary: PACAP-38 from African catfish enhances antibacterial activity and modulates gene expression of immune-related molecules, suggesting its potential as an antimicrobial agent in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Cell Biology
Agnes Futterer, Amaia Talavera-Gutierrez, Tirso Pons, Jesus de Celis, Julio Gutierrez, Veronica Dominguez Plaza, Carlos Martinez-A
Summary: The truncation of the DIDO gene affects RNA splicing and transcription termination in ESC and MEF, altering gene expression involved in differentiation and reprogramming. DIDO3 interacts with the helicase DHX9, leading to increased nuclear R-loop content and DNA replication stress. These defects result in failure of ESC differentiation and MEF reprogramming.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Lorena Magraner-Pardo, Roman A. Laskowski, Tirso Pons, Janet M. Thornton
Summary: Comparing germline and somatic variants in DDR genes reveals that somatic variants in metastatic cases are more likely to be located in Pfam domains and protein interaction interfaces, providing insight into the recurrent affected regions in DDRs.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Lorena Magraner-Pardo, Dino Gobelli, Miguel A. de la Fuente, Tirso Pons, Maria Simarro
Summary: The FASTK family of proteins play a crucial role in post-transcriptional regulation of mitochondrial gene expression. Genetic alterations are commonly observed in FASTK and FASTKD3, mainly consisting of amplifications, while FASTKD1 and FASTKD5 are frequently mutated. Upregulation of mRNA levels of all members of FASTK family is seen in esophageal, stomach, liver and lung cancers. The interaction of FASTK proteins with cancer signaling pathways highlights their potential as diagnostic and therapeutic targets for certain types of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Pedro L. Ramos-Gonzalez, Tirso Pons, Camila Chabi-Jesus, Gabriella Dias Arena, Juliana Freitas-Astua
Summary: The study delves into the relatively unknown biochemical properties of the proteins P15 encoded by cileviruses and their potential importance for viruses within the genus. The research suggests that ORFs p15 may be under purifying selection and exhibit biased codon usage in some viral strains, possibly indicating adaptation to plant hosts. Furthermore, despite high sequence divergence, proteins P15 show a conserved motif and a common predicted 3D structure, hinting at elements of common ancestry and conserved functionality across members of the family.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Guillem Paniagua, Harrys K. C. Jacob, Oksana Brehey, Sara Garcia-Alonso, Carmen G. Lechuga, Tirso Pons, Monica Musteanu, Carmen Guerra, Matthias Drosten, Mariano Barbacid
Summary: KSR1 and KSR2 play important roles in the MAPK pathway, and overexpression of KSR1 or KSR2 can independently activate the pathway and induce cell proliferation. KSR1 requires dimerization with members of the RAF family to stimulate cell proliferation and decreases dependence on KRAS oncogenic signaling.
MOLECULAR ONCOLOGY
(2022)
Article
Immunology
Ileanet Avalos, Thailin Lao, Elsa Maria Rodriguez, Yasser Zamora, Alianet Rodriguez, Ailyn Ramon, Gilda Lemos, Ania Cabrales, Monica Bequet-Romero, Dionne Casillas, Ivan Andujar, Luis Ariel Espinosa, Luis Javier Gonzalez, Yanitza Alvarez, Yamila Carpio, Mario Pablo Estrada
Summary: COVID-19 is a highly contagious respiratory viral disease caused by the novel coronavirus SARS-CoV-2. Researchers have developed a recombinant protein by fusing the receptor-binding domain (RBD) of the viral protein spike (S) with the extracellular domain of CD154, an immune system modulator molecule. This recombinant protein has shown promising immunogenicity in mice and non-human primates, inducing a specific and high IgG response and neutralizing the virus. It has the potential to enhance the current vaccines against COVID-19.
Article
Oncology
Jesus Reyes-Gonzalez, Francisco Barajas-Olmos, Humberto Garcia-Ortiz, Lorena Magraner-Pardo, Tirso Pons, Sergio Moreno, Lucinda Aguirre-Cruz, Andy Reyes-Abrahantes, Angelica Martinez-Hernandez, Cecilia Contreras-Cubas, Jorge Barrios-Payan, Henry J. Ruiz-Garcia, Rogelio Hernandez-Pando, Alfredo Quinones-Hinojosa, Lorena Orozco, Maria del Carmen Abrahantes-Perez
Summary: This study investigates the treatment and prognosis of glioblastoma using a multi-omics approach. It identifies a molecular signature related to brain radiation toxicity and determines the genes and proteins associated with prognosis in glioblastoma patients. The study also uncovers potential novel therapeutic targets and explores drug repositioning possibilities.
Article
Biochemistry & Molecular Biology
Yasel Guerra, Vinicio Armijos-Jaramillo, Tirso Pons, Eduardo Tejera, Colin Berry
Summary: The Kunitz-Soybean Trypsin Inhibitor (Kunitz-STI) family is a large family of proteins with most of its members being protease inhibitors. This study analyzed the crystallographic structures of Kunitz-STI inhibitors to characterize the structural features and locations of serine protease-binding loops. The findings suggest a relationship between the conformation of these loops, the inhibition mechanism, and the plant source of the inhibitors. This research challenges the perception that all Kunitz-STI inhibitors are canonical and will aid in the identification and design of multifunctional proteins.
Article
Genetics & Heredity
Pilar Mur, Julen Viana-Errasti, Sandra Garcia-Mulero, Lorena Magraner-Pardo, Ines G. Munoz, Tirso Pons, Gabriel Capella, Marta Pineda, Lidia Feliubadalo, Laura Valle
Summary: Recommendations have been developed for the classification of non-disruptive variants located in the exonuclease domain of POLE and POLD1, based on the ACMG/AMP criteria. A total of 128 exonuclease domain variants reported in the literature and/or public databases were classified, with 17 variants classified as pathogenic or likely pathogenic, and 17 as benign or likely benign. These recommendations aim to improve diagnostic performance and prevent variant misclassifications in the clinical and scientific communities.
Article
Fisheries
Janet Velazquez, Lynn Cruz, Maylin Perez-Bernal, Onel Valdivia, Arlette Haidar, Alianet Rodriguez, Fidel Herrera, Osmany Gonzalez, Antonio Morales, Lisbet Ulloa, Reinaldo Blanco, Joel Perez, Dayami Dorta, Yaramis Luna, Hilda Elisa Garay, David Diago Abreu, Yassel Ramos, Vladimir Besada, Yeosvany Cabrera, Mario Pablo Estrada, Yamila Carpio
Summary: In this study, a monoclonal antibody (mAb) specific to Nile tilapia IgT was generated and confirmed for its specificity. The mAb showed immunoreactivity against recombinant IgT fragment and native IgT in skin mucus. The percentage of IgT+ B cell populations differed between juveniles and adults and after immunostimulation. This mAb serves as an important immunological tool for studying the biological function and structural characteristics of tilapia IgT.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Pilar Mur, Elsa Ezquerro, Sandra Garcia-Mulero, Lorena Magraner-Pardo, Marta Pineda, Lidia Feliubadalo, Gabriel Capella, Rebeca Sanz, Conxi Lazaro, Tirso Pons, Laura Valle
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Elsa Ezquerro, Pilar Mur, Sandra Garcia-Mulero, Lorena Magraner-Pardo, Rebeca Sanz-Pamplona, Tirso Pons, Gabriel Capella, Laura Valle
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
