期刊
EXPERIMENTAL PARASITOLOGY
卷 127, 期 2, 页码 351-356出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.08.019
关键词
Next generation sequencing; Teladorsagia circumcincta; Gene expression; Ivermectin; Anthelmintic resistance
类别
资金
- Joint Levy Boards
- Scottish Government Rural and Environment Research and Analysis Directorate (RERAD)
- Quality Meat Scotland
- English Beef and Lamb Executive
- Hybu Cig Cymru - Meat Promotion Wales [7382]
The emergence and spread of anthelmintic resistance in parasitic nematodes is a serious threat to the sustainability of the livestock industry. Resistance has a genetic component but the underlying mechanisms and the means by which resistant parasites survive anthelmintic treatment are still poorly understood. Differential gene expression may be implicated, especially in multi-drug resistant parasites. In this study, we investigated the transcriptomic response of a triple drug-resistant isolate of Teladorsagia circumcincta to ivermectin exposure in vitro, using Roche 454 sequencing. The study generated similar to 100,000 new EST sequences, similar to 50,000 each from the ivermectin-exposed and -unexposed pools of parasites. Bioinformatic analysis of the expression profiles revealed statistically significant differences in the mean expression levels of four KEGG orthologous groups, namely 'translation', 'amino acid metabolism', 'carbohydrate metabolism' and 'xenobiotic degradation and metabolism'. Notably, candidate resistance genes such as p-glycoproteins and cytochrome P450s were poorly represented in both datasets. Clusters of sequences, containing both exposed and unexposed ESTs, also revealed statistically significant differences. Four clusters were identified as cytochrome c oxidase subunits, two of these clusters had a statistically significant increase in the number of exposed ESTs compared to unexposed ESTs. Four clusters were identified as vitellogenin; three of these clusters had a statistically significant decrease in number of exposed ESTs compared to unexposed ESTs. (C) 2010 Elsevier Inc. All rights reserved.
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