期刊
EXPERIMENTAL PARASITOLOGY
卷 125, 期 3, 页码 251-255出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.01.024
关键词
Acetylcholinesterase; Butyrylcholinesterase; Protozoa; Rat; Trypanosomosis
类别
The aim of this study was to evaluate cholinesterase activity during the early acute phase of Trypanosoma evansi infection in rats. Fifteen male Wistar rats were randomly distributed into three groups (n = 5 animals per group): two trypanosome-infected groups (T3 and T5) and uninfected controls (C). The animals were inoculated intraperitoneally with 10(6) trypanosomes. The blood was collected by cardiac puncture on the 3rd (T3) or 5th day post-infection (T5 and C). Cerebrum and cerebellum were removed for the evaluation of acetylcholinesterase (AChE) activity. AChE activity was also evaluated in whole blood and butyrylcholinesterase activity (BUChE) in plasma samples. Parasitemia were progressive increase and parasites were observed in the peripheral blood of all infected animals one day post-inoculation. AChE activity was not altered in cerebrum and cerebellum tissues. AChE activity in blood significantly decreased in the T3 and T5 groups (26.63 and 25.86 mU/l mol Hb) compared with the control (37.84 mU/l mol Hb). In addition BUChE activity in plasma was lower in the T3 (7.01 mu mol BTC hydrolyzed/h/mL) than the T5 and C groups (9.84 and 12.00 mu mol BTC hydrolyzed/h/mL). This study therefore, shows that reductions in the activity of cholinesterase occur in acute infection by T. evansi in rats and this demonstrates an important change occurring in animals infected by the protozoan and may indicate a potential role the enzymes play in the mechanism of disease. (C) 2010 Elsevier Inc. All rights reserved.
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