Article
Parasitology
Davi Alvarenga Lima, Leilane Oliveira Goncalves, Joao Luis Reis-Cunha, Paul Anderson Souza Guimaraes, Jeronimo Conceicao Ruiz, Daniel Barbosa Liarte, Silvane Maria Fonseca Murta
Summary: This study performed comparative transcriptomic analysis of wildtype and benznidazole-resistant T. cruzi populations, revealing metabolic pathways related to clinical drug resistance and identifying promising molecular targets for the development of new drugs for Chagas disease.
PARASITES & VECTORS
(2023)
Article
Immunology
Juliana Magalhaes Chaves Barbosa, Yasmin Pedra-Rezende, Luiza Dantas Pereira, Tatiana Galvao de Melo, Helene Santos Barbosa, Joseli Lannes-Vieira, Solange Lisboa de Castro, Anissa Daliry, Kelly Salomao
Summary: The combination treatment of Bz + AMD can attenuate the damage caused by T. cruzi infection in cardiac cells and have a certain control effect on parasite replication.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cassiano Cunha de Souza, Jose Aleixo de Azevedo-Franca, Emile Barrias, Stephany C. F. Cavalcante, Eduardo Guimaraes Vieira, Ana Maria Da Costa Ferreira, Wanderley de Souza, Maribel Navarro
Summary: Four novel metal-BZN complexes were synthesized and characterized in this study. The IC50 values of these complexes in inhibiting parasite proliferation stages are five to ten times lower than benznidazole itself. The cytotoxicity in human cells is lower for these complexes compared to BZN, indicating higher selectivity.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Microbiology
Suzana Marques de Jesus, Leonardo Pinto, Fernanda de Lima Moreira, Glauco Henrique Balthazar Nardotto, Rodrigo Cristofoletti, Luisa Perin, Katia da Silva Fonseca, Pauliana Barbedo, Lorena Cera Bandeira, Paula Melo de Abreu Vieira, Claudia Martins Carneiro
Summary: Chronic infection with Trypanosoma cruzi alters the pharmacokinetics and tissue distribution of benznidazole in mice, potentially impacting the therapeutic dosing regimen. This study suggests that chronic Chagas disease patients may require adjustments in benznidazole pharmacokinetics and dosing due to changes in drug exposure and tissue distribution.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Juan M. Bustamante, Brooke E. White, Gregory K. Wilkerson, Carolyn L. Hodo, Lisa D. Auckland, Wei Wang, Stephanie McCain, Sarah A. Hamer, Ashley B. Saunders, Rick L. Tarleton
Summary: This study demonstrates that higher dose, intermittent administration of benznidazole can effectively treat Trypanosoma cruzi infection. Administration twice a week, for more than 4 months, provides the best chance for parasitological cure.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Caue Benito Scarim, Francisco Olmo, Elizabeth Igne Ferreira, Chung Man Chin, John M. Kelly, Amanda Fortes Francisco
Summary: The research indicates that NFOH has stronger anti-Trypanosoma cruzi activity, works through a inhibitory mechanism, and is more effective in treating chronic infections compared to the acute stage, sharing similar characteristics with BZN.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Katia da Silva Fonseca, Luisa Perin, Nivia Carolina Nogueira de Paiva, Beatriz Cristiane da Silva, Thays Helena Chaves Duarte, Flavia de Souza Marques, Guilherme de Paula Costa, Israel Molina, Rodrigo Correa-Oliveira, Paula Melo de Abreu Vieira, Claudia Martins Carneiro
Summary: The study found that different treatment regimens have varying efficacy on mice infected with different strains, leading to differences in treatment outcomes and side effects. Improving treatment adherence may help enhance the effectiveness of Chagas disease treatment.
Article
Microbiology
Juliana Magalhaes Chaves Barbosa, Yasmin Pedra Rezende, Tatiana Galvao de Melo, Gabriel de Oliveira, Cynthia Machado Cascabulho, Evelyn Nunes Goulart da Silva Pereira, Anissa Daliry, Kelly Salomao Salem
Summary: Chagas disease, caused by Trypanosoma cruzi, affects millions of people in Latin America. The current treatment options have limitations, and combined therapy with amiodarone and benznidazole shows promise in reducing parasitemia and improving cardiac damage in acutely infected mice.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Alina E. Perrone, Mariana Pinillo, Marcela S. Rial, Marisa Fernandez, Natalia Milduberger, Carolina Gonzalez, Patricia L. Bustos, Laura E. Fichera, Susana A. Laucella, Maria Cecilia Albareda, Jacqueline Bua
Summary: This study found that Trypanosoma cruzi, the causative agent of Chagas disease, secretes a 19kDa cyclophilin called TcCyP19, which is recognized by sera from infected mice and patients. The levels of anti-TcCyP19 antibodies significantly decreased in treated mice in the acute and chronic phase of infection, as well as in chronically infected individuals after 12 months of treatment. These findings suggest that TcCyP19 could serve as an early biomarker for the efficiency of trypanocidal treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Julian Ernesto Nicolas Gulin, Margarita Maria Catalina Bisio, Daniela Rocco, Jaime Altcheh, Maria Elisa Solana, Facundo Garcia-Bournissen
Summary: This study evaluates the efficacy of Miltefosine (MLT) as a monodrug and combined with benznidazole (BZ) for treating Trypanosoma cruzi infection. MLT showed promising results in inhibiting the parasite in both in vitro and in vivo models, with improved efficacy when combined with BZ. This study provides support for the potential use of MLT in Chagas disease treatment and the exploration of combination therapies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Angel M. Padilla, Wei Wang, Tsutomu Akama, David S. Carter, Eric Easom, Yvonne Freund, Jason S. Halladay, Yang Liu, Sarah A. Hamer, Carolyn L. Hodo, Gregory K. Wilkerson, Dylan Orr, Brooke White, Arlene George, Huifeng Shen, Yiru Jin, Michael Zhuo Wang, Susanna Tse, Robert T. Jacobs, Rick L. Tarleton
Summary: A newly discovered benzoxaborole prodrug, AN15368, has been found to effectively cure Trypanosoma cruzi infection in mice and naturally infected non-human primates, making it a promising candidate for the prevention and treatment of Chagas disease.
NATURE MICROBIOLOGY
(2022)
Article
Immunology
Priscila Silva Grijo Farani, Khodeza Begum, Glaucia Vilar-Pereira, Isabela Resende Pereira, Igor C. Almeida, Sourav Roy, Joseli Lannes-Vieira, Otacilio Cruz Moreira
Summary: Research explored the immune response-related gene expression changes in heart tissues of C57BL/6 mice chronically infected with T. cruzi and treated with benznidazole (Bz) or Bz+pentoxifylline (PTX). The study found that treatment could mitigate the Th1-driven response in cardiac remodeling processes of CCC patients by restoring the expression of genes related to inflammatory response, cellular development, growth, proliferation, and tissue development pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Antonia Carla de Jesus Oliveira, Emilliany Barbara Silva, Thaisa Cardoso de Oliveira, Fabio de Oliveira Silva Ribeiro, Daniella Nadvorny, Johny Wysllas de Freitas Oliveira, Ana Borrego-Sanchez, Klinger Antonio da Franca Rodrigues, Marcelo Sousa Silva, Pedro Jose Rolim-Neto, Cesar Viseras, Edson C. Silva-Filho, Durcilene Alves da Silva, Luise Lopes Chaves, Monica Felts de La Roca Soares, Jose Lamartine Soares-Sobrinho
Summary: Nanotechnology has led to new and creative applications of nano-medicine, and polymeric nanoparticles have become increasingly important in pharmaceutical applications. This study investigated the use of cashew phthalate gum (PCG) as a particle-forming polymer, which showed pH-sensitive behavior and control over drug release. The developed nanoparticles carrying benznidazole exhibited enhanced biological activity, reduced cytotoxicity, high anti-T. cruzi performance, and pH-responsive release.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Parasitology
Ruben Martin-Escolano, Maria Jose Rosales, Clotilde Marin
Summary: The T. cruzi Arequipa strain is characterized and evaluated as a model for drug discovery in Chagas disease. The study provides insights into the infection characteristics and benznidazole susceptibility of this strain, suggesting its potential for screening new compounds with anti-parasitic properties.
Article
Parasitology
Estefania Prochetto, Ivan Bontempi, Luz Rodeles, Gabriel Cabrera, Miguel Vicco, Paula Cacik, Maria Florencia Pacini, Monica Perez Gianeselli, Ana Rosa Perez, Ivan Marcipar
Summary: The study demonstrates the potential role of a new therapeutic vaccine in the treatment of chronic Chagas disease. The results show that the vaccine can reduce the progression of heart disease and decrease heart parasite load.
Article
Chemistry, Applied
Policarpo Ademar Sales Junior, Carlos Leomar Zani, Ezequias Pessoa de Siqueira, Markus Kohlhoff, Fatima Regina Marques, Alisson Samuel Portes Caldeira, Betania Barros Cota, Daniela Nabak Bueno Maia, Luiza Guimaraes Tunes, Silvane Maria Fonseca Murta, Tania Maria Almeida Alves
Summary: Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, lacks effective treatment options, highlighting the need for new compounds for chemotherapy. Two new trixikingolides isolated from the plant Trixis vauthieri show promising in vitro activity against T. cruzi, indicating potential as a more potent alternative to existing drugs. Further testing on infected mice is the next step to determine their effectiveness.
NATURAL PRODUCT RESEARCH
(2021)
Article
Mycology
Mayara B. Ogaki, Daniela R. Teixeira, Rosemary Vieira, Juan M. Lirio, Joao P. S. Felizardo, Rodrigo C. Abuchacra, Renan P. Cardoso, Carlos L. Zani, Tania M. A. Alves, Policarpo A. S. Junior, Silvane M. F. Murta, Emerson C. Barbosa, Jaquelline G. Oliveira, Isabela P. Ceravolo, Patricia O. Pereira, Carlos A. Rosa, Luiz H. Rosa
Article
Chemistry, Medicinal
Camila Capelini, Vitoria R. F. Camara, Jose D. Figueroa Villar, Juliana M. C. Barbosa, Kelly Salomao, Solange L. de Castro, Policarpo A. S. Junior, Silvane M. F. Murta, Thais B. Couto, Maria C. S. Lourenco, James L. Wardell, John N. Low, Edson F. da Silva, Samir A. Carvalho
Summary: In this study, seventeen coumarinic N-acylhydrazonic derivatives were synthesized and evaluated for their antitrypanosomal and antimycobacterial activities. The compounds showed moderate activity against T. cruzi and meaningful activity against resistant strains of M. tuberculosis. These compounds acting as multitarget could be potential leads for the development of new trypanocidal and bactericidal agents.
MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Gabriela Maciel Diogo, Josimara Souza Andrade, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Viviane Martins Rebello Dos Santos, Jason Guy Taylor
Article
Biochemistry & Molecular Biology
Graciele Cunha Alves de Menezes, Barbara Alves Porto, Soraya Sander Amorim, Carlos Leomar Zani, Tania Maria de Almeida Alves, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Jefferson Cardia Simoes, Betania Barros Cota, Carlos Augusto Rosa, Luiz Henrique Rosa
Article
Biochemistry & Molecular Biology
Mayara B. Ogaki, Rosemary Vieira, Marcelo C. Muniz, Carlos L. Zani, Tania M. A. Alves, Policarpo A. S. Junior, Silvane M. F. Murta, Emerson C. Barbosa, Jaquelline G. Oliveira, Isabela P. Ceravolo, Patricia O. Pereira, Carlos A. Rosa, Luiz H. Rosa
Article
Immunology
Elvira Cynthia Alves Horacio, Jessica Hickson, Silvane Maria Fonseca Murta, Jeronimo Conceicao Ruiz, Laila Alves Nahum
Summary: Neglected Tropical Diseases, including Leishmaniasis, present challenges to traditional research methods due to their complexity. Systems biology offers a promising approach to understanding drug resistance in Leishmania and developing new treatment strategies. Antioxidant defense enzymes and other biomarkers are potential targets for drug development against leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Camila Capelini, Katia R. de Souza, Juliana M. C. Barbosa, Kelly Salomao, Policarpo A. Sales Junior, Silvane M. F. Murta, Solange M. S. V. Wardell, James L. Wardell, Edson F. da Silva, Samir A. Carvalho
Summary: In this study, a new series of phenoxyacetohydrazones derivatives were designed, synthesized, and evaluated for their antitrypanosomal and cytotoxic activities. Compound 11 showed activity against trypomastigotes equivalent to benznidazole, while compound 19 exhibited significantly higher activity against infected cultures compared to the standard drug.
MEDICINAL CHEMISTRY RESEARCH
(2021)
Article
Parasitology
Ana Maria Murta Santi, Paula Alves Silva, Isabella Fernandes Martins Santos, Silvane Maria Fonseca Murta
Summary: FeSOD-A is essential in L. infantum and plays a crucial role in defense against oxidative stress and infectivity. FeSOD-A-deficient parasites deregulate metabolic pathways related to antimony and miltefosine resistance.
PARASITES & VECTORS
(2021)
Article
Chemistry, Medicinal
Jessica V. Faria, Fernanda P. Z. Passos, Paulo H. A. da Costa, Andressa P. de Oliveira, Yasmin O. D. da Cruz, Frederico S. Castelo-Branco, Maria C. S. Lourenco, Silvane M. F. Murta, Policarpo A. S. Junior, Alice M. R. Bernardino, Monica M. Bastos, Nubia Boechat
Summary: This study designed and synthesized two new series of compounds and evaluated their activities against Chagas disease and tuberculosis. The results showed that one of the compounds had important activity against both diseases. This study reinforces the importance of this compound in antimicrobial activity and provides a starting point for the development of new drugs.
MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Ana Maria Murta Santi, Juliana Martins Ribeiro, Joao Luis Reis-Cunha, Gabriela de Assis Burle-Caldas, Isabella Fernandes Martins Santos, Paula Alves Silva, Daniela de Melo Resende, Daniella Castanheira Bartholomeu, Santuza Maria Ribeiro Teixeira, Silvane Maria Fonseca Murta
Summary: In this study, the deletion of PGFS in T. cruzi did not affect the parasites' susceptibility to drugs but resulted in changes in oxidative stress tolerance and infectivity.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Eduardo Barbieri, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Barbara Silva
Summary: Pyrrole compounds were synthesized using the Knorr method and various derivatives were obtained by transesterification reactions. The composition of the alpha group in relation to the nitrogen heteroatom of the pyrrole nuclei was varied through acid hydrolysis, decarboxylation, electrophilic aromatic substitution, and reaction with thiosemicarbazide. Four synthesized compounds showed anti-Trypanosoma cruzi activity with IC50 values ranging from 14.1 to >50 μM.
REVISTA VIRTUAL DE QUIMICA
(2022)
Article
Parasitology
Ana Maria Murta Santi, Silvane Maria Fonseca Murta
Summary: Chagas disease and leishmaniasis are neglected tropical diseases caused by parasitic protozoan Trypanosoma cruzi and Leishmania spp. There is currently no human vaccine available and treatment options are limited. This study focuses on the antioxidant defense mechanisms and the role of specific enzymes in oxidative stress defense and drug resistance in T. cruzi and Leishmania spp.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2022)
Article
Chemistry, Multidisciplinary
Lucas R. D. Sousa, Maria Luiza S. Azevedo, Dayana F. Rocha, Angela L. Andrade, Tatiane R. Amparo, Orlando D. H. dos Santos, Janaina B. Seibert, Luciano R. Pereira, Paula M. A. Vieira, Claudia M. Carneiro, Ana Paula M. Barboza, Bernardo R. A. Neves, Policarpo A. Sales Jr, Silvane M. F. Murta, Katia M. Novack, Viviane M. R. dos Santos
Summary: This study modified the PEG 4000 polymer and incorporated benznidazole, confirmed by NMR analysis, observed film morphology by AFM, and increased system stability with acetylation of PEG. The encapsulation of benznidazole in microparticles enhanced its dissolution profile, leading to higher trypanocidal effect.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Josimara Souza Andrade, Leonardo Gomes de Abreu, Policarpo Ademar Sales Junior, Silvane Maria Fonseca Murta, Jason Guy Taylor
Summary: The synthesis and evaluation of flavones as trypanocidal agents was conducted, with some compounds showing modest activity against T cruzi, particularly those with nitro or methoxy substituents. Structural modifications at position 3 of the dihydropyrone ring resulted in the most active flavone, indicating potential for promising new compounds with trypanocidal properties.
REVISTA VIRTUAL DE QUIMICA
(2021)
