Article
Multidisciplinary Sciences
Tianyan Liu, Till Stephan, Peng Chen, Jan Keller-Findeisen, Jingting Chen, Dietmar Riedel, Zhongtian Yang, Stefan Jakobs, Zhixing Chen
Summary: This study reports a mitochondrial inner membrane fluorescent marker, PK Mito Orange (PKMO), which features reduced phototoxicity and high stability, enabling super-resolution recordings of mitochondrial inner membrane dynamics. The PKMO dye is optically orthogonal with green and far-red markers, allowing multiplexed recordings. By using multi-color STED microscopy, interactions between mitochondria and other cellular components can be captured at sub-100 nm resolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Clara-Marie Guerth, Maria Augusta do Rego Barros Fernandes Lima, Victor Macarron Palacios, Angel Rafael Cereceda Delgado, Jasmine Hubrich, Elisa D'Este
Summary: This study quantified the occurrence of neurofilament isoforms at the postsynapse and compared them with markers of synaptic strength and activity. The results showed that different isoforms rarely colocalize with each other within dendritic spines, and NFL is the most abundant isoform. The presence of neurofilaments at the postsynapse correlates with synaptic status, shedding new light on their regulation and possible contribution to synaptopathies.
Article
Multidisciplinary Sciences
Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini
Summary: The feasibility of super-resolution microscopy for live and thick sample imaging is limited. We propose a method that combines ISM and STED techniques to achieve high-resolution imaging without introducing photodamage and background degradation.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Roman Schmidt, Tobias Weihs, Christian A. Wurm, Isabelle Jansen, Jasmin Rehman, Steffen J. Sahl, Stefan W. Hell
Summary: The MINFLUX concept has advanced the resolution of fluorescence microscopy to molecular dimensions, achieving 1-3 nm resolution in three dimensions. This widely applicable technique allows for nanometer-precise and accurate real-time localization of individually activated fluorophores, with potential applications in cellular and neurobiological research.
NATURE COMMUNICATIONS
(2021)
Review
Anatomy & Morphology
Samira Jadavi, Paolo Bianchini, Ornella Cavalleri, Silvia Dante, Claudio Canale, Alberto Diaspro
Summary: Atomic force microscopy (AFM) is a nano-mechanical tool used for biological studies at the molecular scale, measuring surface topography, mechanical, and electrical properties. Integrating AFM with super-resolved fluorescence microscopy addresses the resolution mismatch issue, while also utilizing the label-free imaging capabilities of AFM to complement fluorescence images. Recent research highlights the importance of combining these techniques in biological research for better specificity and resolution.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Multidisciplinary Sciences
Xin Guo, Rui Pu, Zhimin Zhu, Shuqian Qiao, Yusen Liang, Bingru Huang, Haichun Liu, Lucia Labrador-Paez, Uliana Kostiv, Pu Zhao, Qiusheng Wu, Jerker Widengren, Qiuqiang Zhan
Summary: The authors introduce a new nanoscopy technique called stimulated-emission induced excitation depletion (STExD), which uses a single pair of low-power, near-infrared, continuous-wave lasers to inhibit the emission of multichromatic probes. By manipulating the common sensitizer in lanthanide upconversion systems, emission inhibition is achieved for a wide range of emitters, with high depletion efficiency and low saturation intensity.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Koen J. A. Martens, Abbas Jabermoradi, Suyeon Yang, Johannes Hohlbein
Summary: In single-molecule localization microscopy, integrating various PSF modalities into the phasor-based framework enables fast and accurate localization of single-molecule emitters in three dimensions. This approach is crucial for analyzing complex PSFs and achieving high precision in localization.
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucas A. Saavedra, Hector Buena-Maizon, Francisco J. Barrantes
Summary: The study characterized the distribution of nAChRs receptors on the plasma membrane of CHO-K1/A5 cells using two labeling and two super-resolution microscopy strategies. It was found that antibody-induced crosslinking of receptors resulted in nanoclusters with higher densities. STORM and STED provided complementary information on the distribution of nAChRs at different scales.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Leonid Andronov, Rachel Genthial, Didier Hentsch, Bruno P. Klaholz
Summary: The development of an image splitter based multi-color single-molecule localization microscopy method (splitSMLM) in combination with a spectral demixing algorithm improves localization accuracy as exemplified by three-color imaging of nuclear pore complex proteins.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biophysics
Tong Zhu, Guanqing Yang, Xiaolu Liu, Pan Xiang, Zhenghui Yang, Sijing Zhang, Juan Chen, Hong Wang, Senio Campos de Souza, Zhongping Zhang, Ruilong Zhang, Yupeng Tian, Jieying Wu, Xiaohe Tian
Summary: By rationally constructing a Py-BODIPY derivative, researchers successfully targeted the mitochondrial membrane in living cells without disturbing its physiological microenvironments, showing strong internalization capabilities in pre-fixed and stimulus disrupted samples. The probe's cristae specificity and excellent photostability allow for the observation of mitochondrial dynamic nano-structures with unprecedented resolution, demonstrating how mitochondrial 3D ultrastructure evolves under oxidative phosphorylation conditions.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biology
Waja Wegner, Heinz Steffens, Carola Gregor, Fred Wolf, Katrin Willig, Yukiko Goda
Summary: This study investigated the effects of environmental enrichment on the morphology and dynamics of individual synapses. The results showed that environmental enrichment can enhance the uniformity of synaptic strength and lead to a more dynamic topography of the PSD95 nanoorganization.
Article
Neurosciences
Stefano Calovi, Federico N. Soria, Jan Tonnesen
Summary: STED microscopy is a super-resolution technique that allows imaging at higher spatial resolution than the diffraction limit of light, particularly well-suited for imaging inside live brain tissue. Over the past 20 years, STED microscopy has undergone extensive developments and facilitated remarkable neurophysiological discoveries.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Optics
Pierre Jouchet, Clement Cabriel, Nicolas Bourg, Marion Bardou, Christian Pous, Emmanuel Fort, Sandrine Leveque-Fort
Summary: The modulated localization microscopy achieves high molecular localization precision through an illumination modulation method, improving axial localization precision while maintaining fast acquisition speed. Compared to existing super-resolution fluorescence microscopy, this method offers unique advantages.
Review
Biochemistry & Molecular Biology
Jon Ander Nieto-Garai, June Olazar-Intxausti, Itxaso Anso, Maier Lorizate, Oihana Terrones, Francesc-Xabier Contreras
Summary: Interorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. The study of MCS is important due to its implication in various biological functions. Super-resolution fluorescence microscopy (SRFM) has emerged as a powerful tool for studying MCS. This review explores the characteristics and biological functions of MCS, summarizes different approaches for studying them, and focuses on the use of SRFM techniques in MCS research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physics, Applied
Julie Angibaud, Patrice Mascalchi, Christel Poujol, U. Valentin Nagerl
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2020)
Article
Biochemical Research Methods
Florian Levet, Jan Tonnesen, U. Valentin Nagerl, Jean-Baptiste Sibarita
Review
Neurosciences
Teresa Sierra-Arregui, Javier Llorente, Paula Gimenez Minguez, Jan Tonnesen, Olga Penagarikano
Article
Neurosciences
Christian Henneberger, Lucie Bard, Aude Panatier, James P. Reynolds, Olga Kopach, Nikolay I. Medvedev, Daniel Minge, Michel K. Herde, Stefanie Anders, Igor Kraev, Janosch P. Heller, Sylvain Rama, Kaiyu Zheng, Thomas P. Jensen, Inmaculada Sanchez-Romero, Colin J. Jackson, Harald Janovjak, Ole Petter Ottersen, Erlend Arnulf Nagelhus, Stephane H. R. Oliet, Michael G. Stewart, U. Valentin Nagerl, Dmitri A. Rusakov
Article
Neurosciences
Cordelia Imig, Francisco Jose Lopez-Murcia, Lydia Maus, Ines Hojas Garcia-Plaza, Lena Suenke Mortensen, Manuela Schwark, Valentin Schwarze, Julie Angibaud, U. Valentin Naegerl, Holger Taschenberger, Nils Brose, Benjamin H. Cooper
Editorial Material
Cell Biology
Julien Cassereau, Vincent Courdavault, U. Valentin Nagerl
Summary: Fluorescent proteins have become essential tools in biological research for labeling and sensing cells and proteins. Campbell et al. engineered a new GFP variant with enhanced brightness and stability, enabling advanced cellular bioimaging applications in neuroscience and beyond.
TRENDS IN CELL BIOLOGY
(2021)
Article
Neurosciences
Misa Arizono, V. V. G. Krishna Inavalli, Stephane Bancelin, Monica Fernandez-Monreal, U. Valentin Nagerl
Summary: The extracellular space in the brain, controlled by astrocytes, is crucial for maintaining proper brain homeostasis and neuronal communication. Understanding the microanatomical relationships between astrocytes, neuropil, and the ECS could provide insights into neuron-glia communication in various physiological and pathological conditions. Using advanced imaging techniques, researchers were able to visualize the dynamic interactions between astrocyte microstructures and cellular components in live brain tissue, shedding light on potential implications for conditions such as LTP induction, epileptic seizures, and acute ischemic stroke.
News Item
Medicine, Research & Experimental
Misa Arizono, Agata Idziak, U. Valentin Nagerl
M S-MEDECINE SCIENCES
(2021)
Article
Neurosciences
Stefano Calovi, Federico N. Soria, Jan Tonnesen
Summary: STED microscopy is a super-resolution technique that allows imaging at higher spatial resolution than the diffraction limit of light, particularly well-suited for imaging inside live brain tissue. Over the past 20 years, STED microscopy has undergone extensive developments and facilitated remarkable neurophysiological discoveries.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Medicine, Research & Experimental
Lucia Mora-Jimenez, Miguel Valencia, Rocio Sanchez-Carpintero, Jan Tonnesen, Saja Fadila, Moran Rubinstein, Manuela Gonzalez-Aparicio, Maria Bunuales, Eva Fernandez-Pierola, Maria Jesus Nicolas, Elena Puerta, Cristina Miguelez, Paula Gimenez Minguez, Sara Lumbreras, Gloria Gonzalez-Aseguinolaza, Ana Ricobaraza, Ruben Hernandez-Alcoceba
Summary: Dravet syndrome, a genetic encephalopathy characterized by severe epilepsy, has limited response to current antiepileptic drugs. Novel therapies aiming to restore gene expression have shown partial success in improving neurological development.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Neurosciences
Stephane Bancelin, Luc Mercier, Emanuele Murana, U. Valentin Nagerl
Summary: The study aimed to develop and validate a method based on adaptive optics for a priori correction of spherical aberrations to improve the spatial resolution of STED microscopy inside thick biological tissue. Experimental results demonstrated that the corrections substantially increased image quality in living brain slices, allowing for clear measurement of small structures at depth.
Review
Neurosciences
Misa Arizono, U. Valentin Nagerl
Summary: Studying neuron-astrocyte communication faces challenges in visualizing the tripartite synapse, where STED microscopy offers nanoscale live imaging opportunities. Despite challenges in live-tissue imaging, STED studies have revealed new insights into the structure and function of the tripartite synapse, shedding light on the role of astrocytes in brain physiology and behavior.
Review
Cell Biology
Misa Arizono, Agata Idziak, Federica Quici, U. Valentin Nagerl
Summary: Brain cells like neurons and astrocytes have complex structures that are often too small to be observed with conventional light microscopy. Super-resolution microscopy can overcome this limitation and allow visualization of the nanoscale mechanisms that govern brain physiology. This review discusses the principles and strengths of different super-resolution microscopy techniques.
TRENDS IN CELL BIOLOGY
(2023)
Article
Neurosciences
Audrey Denizot, Misa Arizono, U. Valentin Nagerl, Hugues Berry, Erik De Schutter
Summary: This study investigates the relationship between astrocytic nanoscale morphology and Ca2+ activity using computational tools and idealized 3D geometries. The results demonstrate that astrocytic morphology significantly influences Ca2+ signals, with swelling hindering signal propagation. This highlights the importance of astrocyte morphology in neuron-astrocyte communication, particularly in pathological conditions.
Article
Neurosciences
Jan Tonnesen, Sabina Hrabetova, Federico N. Soria
Summary: The brain extracellular space (ECS) is a complex interstitial reticulum that plays a crucial role in intercellular communication. Diffusion is the primary mechanism for the transportation of neuroactive substances within the ECS. Recent nanoscopic imaging techniques have revealed the dynamic nature of the ECS and its heterogeneous diffusivity across brain regions. The geometry of the ECS and the properties of the interstitial fluid and extracellular matrix determine the diffusivity, which can be regulated independently.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Neurosciences
Wenzhu Wang, Zihan Li, Yitong Yan, Shuo Wu, Xinyu Yao, Chen Gao, Lanxiang Liu, Yan Yu
Summary: This study investigated the reparative mechanisms of low-intensity pulsed ultrasound (LIPUS) in traumatic brain injury (TBI) and found that LIPUS promotes hippocampal neurogenesis, enhances neural electrical activity and neural plasticity, ultimately restoring neuronal function and cognitive capabilities in TBI mice.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Wenmin Yi, Fei Chen, Minghao Yuan, Chuanling Wang, Shengyuan Wang, Jie Wen, Qian Zou, Yinshuang Pu, Zhiyou Cai
Summary: The study suggests that a high-fat diet may lead to tau hyperphosphorylation and synaptic dysfunction by inhibiting the SIRT1/AMPK pathway and disrupting autophagy flux, ultimately resulting in cognitive decline.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Kim M. Hemsley, Helen Beard, Glyn Chidlow, Teresa Mammone, Leanne K. Winner, Daniel Neumann, Barbara King, Marten F. Snel, Paul J. Trim, Robert J. Casson
Summary: Optical coherence tomography (OCT) is a non-invasive method that can be used to rapidly and quantitatively examine the integrity of the neuroretina. It has been shown that OCT can be used to observe retinal thinning in patients with childhood dementia, and to assess the improvement of retinal structure after treatment. Furthermore, OCT can provide insights into other childhood dementias based on the correlation between retinal and brain degeneration in Sanfilippo syndrome.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Qianling Jiang, Xin Ma, Gaochen Zhu, Wen Si, Lingyu He, Guan Yang
Summary: This study investigated the effects of EAE induction on thymopoiesis and T cell development, revealing changes such as increased apoptosis, decreased proliferation, and a blockade in the transition from double-negative thymocytes to double-positive cells. It was also found that positive selection was disrupted in the thymus of EAE mice, along with an increased production of regulatory T cells.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Alice McDouall, Guido Wassink, Sumudu Ranasinghe, Kelly Q. Zhou, Rashika N. Karunasinghe, Justin M. Dean, Joanne O. Davidson
Summary: This study found that blocking connexin 43 hemichannels can attenuate brain injury and promote neurodevelopment in infants with mild hypoxic-ischemic encephalopathy, without causing hypothermia.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Hannah Sweetman, Mahmudur Rahman, Aditya Vedantam, Kajana Satkunendrarajah
Summary: Degenerative cervical myelopathy (DCM) is a neurological condition characterized by chronic compression of the cervical spinal cord, leading to impaired limb function. While respiratory dysfunction is not a common symptom of DCM, it can affect the ventilatory response to respiratory challenges. Surgical decompression improves sensorimotor function in DCM, but its impact on respiratory function is unclear. This study evaluates respiratory function and adaptive ventilation in a DCM model, showing that DCM impairs acute adaptive ventilatory ability and surgical decompression does not fully restore it.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Chengmei Sun, Muhammad Saif Ur Rahman, Budbazar Enkhjargal, Jianhua Peng, Keren Zhou, Zhiyi Xie, Lingyun Wu, Tongyu Zhang, Qiquan Zhu, Jiping Tang, Yujia Zeng, John H. Zhang, Shanshan Xu
Summary: This study found that Osteopontin (OPN) can attenuate inflammatory responses after subarachnoid hemorrhage (SAH) by promoting an anti-inflammatory microglial state. This effect may be mediated through the integrin-FAK-STAT3 and NF-kappa B signaling pathways.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Yang Yang, Xuezhu Chen, Chuanyan Yang, Mei Liu, Qianying Huang, Likun Yang, Yuhai Wang, Hua Feng, Zhongyang Gao, Tunan Chen
Summary: The study explores the effects of specific chemogenetic stimulation of intact corticospinal tract on functional recovery after stroke in mice. The findings demonstrate that combining chemogenetic activation with rehabilitation training leads to significant motor functional recovery by promoting axon sprouting and rewiring new functional circuits.
EXPERIMENTAL NEUROLOGY
(2024)
