Article
Cell & Tissue Engineering
Jeng-Chang Chen
Summary: This study demonstrated the feasibility of minimally invasive rectal transplantation of enteric neural stem cells (ENSC) to achieve long-term and long-range reconstitution of enteric ganglia, providing a potential approach to replace or replenish damaged/missing enteric neurons.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Zhengqing Hu, Li Tao, Meng Deng
Summary: The study found that Nestin and Sox2 double-expressing NSCs were observed in the AC area of mice from P3 to 4M in vivo, while the number of NSCs reduced significantly with age. In vitro, the neurosphere forming capability, cell proliferation, and percentage of Nestin and Sox2 double-expressing NSCs also decreased significantly with age.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jonas Engert, Bjoern Spahn, Linda Bieniussa, Rudolf Hagen, Kristen Rak, Johannes Voelker
Summary: This study aimed to investigate the neural stem cell potential of the medial geniculate body (MGB). The findings showed that MGB cells exhibited the cardinal features of neural stem cells, including self-renewal, formation of progenitor cells, and differentiation into all neuronal lineage cells. These findings contribute to a better understanding of the development of the auditory pathway.
Article
Veterinary Sciences
Warunya Chakritbudsabong, Ladawan Sariya, Phakhin Jantahiran, Nattarun Chaisilp, Somjit Chaiwattanarungruengpaisan, Ruttachuk Rungsiwiwut, Joao N. Ferreira, Sasitorn Rungarunlert
Summary: This study successfully generated porcine induced neural stem cells (piNSCs) from pig fibroblasts using the Sendai virus (SeV) method. The piNSCs exhibited high expression of NSC and proliferation markers, as well as multipotency, without developing induced pluripotent stem cells. These piNSCs have potential applications in disease modeling and regenerative therapies in both human and veterinary medicine.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Endocrinology & Metabolism
Nelly Saber, Cara E. E. Ellis, Diepiriye G. G. Iworima, Robert K. K. Baker, Alireza Rezania, Timothy J. J. Kieffer
Summary: This study investigates the differentiation of human embryonic stem cell-derived pancreatic endoderm cells (PECs) in different implantation sites. It is found that PECs implanted in the TheraCyte device display faster secretion of human C-peptide, while cells implanted under the kidney capsule or in the gonadal fat pad show slower secretion. Interestingly, PECs implanted under the kidney capsule in female mice exhibit faster differentiation to endocrine cells and higher levels of arginine-stimulated glucagon and glucagon-like peptide 1 secretion.
Article
Anatomy & Morphology
Concepcion Rojo Salvador, Maria de Lourdes Galicia Guerrero, Belen Sanchez Maldonado, Alfredo Gonzalez-Gil, Rosa Ana Picazo Gonzalez
Summary: Neurospheres derived from adult stem cells of non-neural tissues show promising potential for autologous cell therapy as a source of neural stem cells and neural progenitor cells. By analyzing molecular expression levels and conducting structural analyses, it was found that neurospheres generated from ovarian cortical cells share structural and ultrastructural characteristics with neurospheres from the central nervous system.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2022)
Article
Neurosciences
Takamasa Kamei, Atsushi Tamada, Toshiya Kimura, Akira Kakizuka, Akio Asai, Keiko Muguruma
Summary: Purkinje cells are crucial for motor coordination and memory integration in the cerebellum. This study investigates the function of human iPS cell-derived cerebellar progenitors in mice with Purkinje cell death. The transplantation of cerebellar organoids showed promising results but further improvements are needed for successful integration. These findings contribute to regenerative medicine for cerebellar degeneration.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Tatiana Subkhankulova, Karen Camargo Sosa, Leonid A. Uroshlev, Masataka Nikaido, Noah Shriever, Artem S. Kasianov, Xueyan Yang, Frederico S. L. M. Rodrigues, Thomas J. Carney, Gemma Bavister, Hartmut Schwetlick, Jonathan H. P. Dawes, Andrea Rocco, Vsevolod J. Makeev, Robert N. Kelsh
Summary: Neural crest cells are highly multipotent stem cells, and it is still unclear how their fate restriction to specific fates occurs. This study shows that in zebrafish, broad multipotency is retained even after migration, suggesting that fate restriction occurs directly but dynamically.
NATURE COMMUNICATIONS
(2023)
Article
Developmental Biology
Anna Lozano-Urena, Laura Lazaro-Carot, Esteban Jimenez-Villalba, Raquel Montalban-Loro, Isabel Mateos-White, Pere Duart-Abadia, Irene Martinez-Gurrea, Keiichi I. Nakayama, Isabel Farinas, Martina Kirstein, Cristina Gil-Sanz, Sacri R. Ferron
Summary: Adult neurogenesis relies on multipotent neural stem cells (NSCs) with unique properties and growth requirements. Although many imprinted genes' roles in adult neurogenesis are still unknown, insulin-like growth factor 2 (IGF2) has been shown to contribute to NSC maintenance and promote terminal differentiation into astrocytes, neurons, and oligodendrocytes. This differentiation effect is mediated by the expression of the maternally expressed gene Cdkn1c, encoding the cell cycle inhibitor p57, and occurs independently of its role in cell-cycle progression, balancing the relationship between astrogliogenesis, neurogenesis, and oligodendrogenesis.
Article
Biochemistry & Molecular Biology
Ye Rim Kim, Si Won Jang, Jae Ho Han, Ga Rim Na, Hoon Jang, Hyun Woo Choi
Summary: Research on the technology for in vitro differentiation of embryonic stem cells (ESCs) into neural lineages is crucial for developmental biology, regenerative medicine, and cell therapy. Co-culturing ESCs with neural stem cells (NSCs) promotes ESC differentiation into ectoderm, with higher expression levels of Pax6 and Sox1 observed. NSC-derived conditioned medium (CM) showed lower differentiation efficiency compared to non-conditioned medium.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Biology
Xin Fu, Qiang He, Yu Tao, Mengdi Wang, Wei Wang, Yalong Wang, Qing Cissy Yu, Fang Zhang, Xiaoyu Zhang, Ye-Guang Chen, Dong Gao, Ping Hu, Lijian Hui, Xiaoqun Wang, Yi Arial Zeng
Summary: Stem cells are crucial undifferentiated cells with the ability of self-renewal and differentiation, playing a significant role in organ and tissue development and repair. Tissue-specific stem cells hold powerful potential for regeneration and are promising for future research in tissue stem cell biology.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
N. Sumru Bayin, Dogukan Mizrak, Daniel N. Stephen, Zhimin Lao, Peter A. Sims, Alexandra L. Joyner
Summary: By analyzing cellular states and genes, different subtypes of NEPs and their lineages were identified, providing new insights into cellular plasticity and repair mechanisms in the brain.
Article
Cell Biology
Michael Claxton, Michela Pulix, Michelle K. Y. Seah, Ralph Bernardo, Peng Zhou, Sultan Aljuraysi, Triantafillos Liloglou, Philippe Arnaud, Gavin Kelsey, Daniel M. Messerschmidt, Antonius Plagge
Summary: Genomic imprinting is an epigenetic process that leads to parent-of-origin specific gene expression. In this study, the researchers investigated the allelic expression biases in the brain and found variable states of allelic expression, especially for Trappc9 and Ago2 genes. They also discovered that Trappc9 and Ago2 are not imprinted in hippocampus-derived neural stem cells. The findings suggest the presence of variability and mosaicism in allelic expression between individual cells of the brain tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Jeng-Chang Chen, Wendy Yang, Li-Yun Tseng, Hsueh-Ling Chang
Summary: In this study, neurospheres were successfully generated using NSM and SRM, and they were able to assemble enteric neural networks in both two- and three-dimensional environments, as well as on gut explants. The transplantation of these neurospheres resulted in the reconstitution of myenteric plexuses in the recipient's rectum. This research provides valuable insights into the dynamic nature of the ENS and opens up possibilities for further exploration and understanding of its cellular activities.
STEM CELL RESEARCH & THERAPY
(2023)
Letter
Oncology
Matthew Cannon, Hannah Phillips, Sidney Smith, Shaneice Mitchell, Kristina Landes, Payal Desai, John Byrd, Rosa Lapalombella
Summary: The in vitro erythrocyte differentiation model is an important tool for studying erythroid development in normal and disease related hematopoiesis. Challenges remain in efficiency of maturation, terminal enucleation, and scalability despite various modified methodologies.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2021)
Article
Clinical Neurology
Andreas-Antonios Roussakis, Zhou Zeng, Nicholas P. Lao-Kaim, Antonio Martin-Bastida, Paola Piccini
Summary: The study found that in moderate-stage Parkinson's disease, asymmetry in striatal dopaminergic degeneration becomes less prominent over time, while the asymmetry of motor symptoms remains consistent between the clinically most and least affected body sides.
JOURNAL OF NEUROLOGY
(2021)
Article
Mathematics, Applied
Vassilis Cutsuridis, Shouyong Jiang, Matt J. Dunn, Anne Rosser, James Brawn, Jonathan T. Erichsen
Summary: The study found that early Huntington's disease patients exhibit slower and more variable responses in eye movement tasks, with higher error rates. The neural model indicates that the more gradual and noisy accumulation of evidence by HD patients is responsible for the prolonged and more variable reaction times observed.
Article
Cell & Tissue Engineering
Narawadee Choompoo, Oliver J. M. Bartley, Sophie V. Precious, Ngoc-nga Vinh, Christian Schnell, Ana Garcia, Victoria H. Roberton, Nigel M. Williams, Paul J. Kemp, Claire M. Kelly, Anne E. Rosser
Summary: The study showed that iPSC derived from human WGE have the potential to differentiate into STR cells and may serve as a donor source for CRT for HD.
Editorial Material
Clinical Neurology
Anne E. Rosser, Lesley Jones
MOVEMENT DISORDERS CLINICAL PRACTICE
(2022)
Review
Clinical Neurology
Anne E. Rosser, Monica E. Busse, William P. Gray, Romina Aron Badin, Anselme L. Perrier, Vicki Wheelock, Emanuele Cozzi, Unai Perpina Martin, Cristina Salado-Manzano, Laura J. Mills, Cheney Drew, Steven A. Goldman, Josep M. Canals, Leslie M. Thompson
Summary: Substantial progress has been made in regenerative medicine strategies for CNS disorders, but there are challenges in translating these strategies to clinical settings, such as validating preclinical data, ensuring quality control of cell products, designing surgical devices, and conducting efficient clinical trials. Addressing these challenges in Huntington's disease can serve as a roadmap for other neurological conditions.
Article
Neurosciences
Branduff McAllister, Jasmine Donaldson, Caroline S. Binda, Sophie Powell, Uroosa Chughtai, Gareth Edwards, Joseph Stone, Sergey Lobanov, Linda Elliston, Laura-Nadine Schuhmacher, Elliott Rees, Georgina Menzies, Marc Ciosi, Alastair Maxwell, Michael J. Chao, Eun Pyo Hong, Diane Lucente, Vanessa Wheeler, Jong-Min Lee, Marcy E. MacDonald, Jeffrey D. Long, Elizabeth H. Aylward, G. Bernhard Landwehrmeyer, Anne E. Rosser, Jane S. Paulsen, Nigel M. Williams, James F. Gusella, Darren G. Monckton, Nicholas D. Allen, Peter Holmans, Lesley Jones, Thomas H. Massey
Summary: This study identifies rare coding variants associated with clinical effects in Huntington's disease (HD) using exome sequencing. Damaging coding variants in candidate modifier genes associated with altered HD onset or severity were discovered. FAN1 nuclease activity slows CAG expansion and is associated with later onset of HD.
NATURE NEUROSCIENCE
(2022)
Article
Clinical Neurology
Lori Quinn, Rebecca Playle, Cheney J. G. Drew, Katie Taiyari, Rhys Williams-Thomas, Lisa Muratori, Katy Hamana, Beth Ann Griffin, Mark Kelson, Robin Schubert, Ciaran Friel, Philippa Morgan-Jones, Anne Rosser, Monica Busse
Summary: This study aimed to evaluate the feasibility of a physical therapist-led PA intervention for patients with Huntington's disease (HD) through a nested randomized controlled trial (RCT) over 12 months. The results showed improvements in fitness, walking endurance, and self-reported PA in the intervention group compared to the control group, while the cohort group had motor and functional decline rates comparable to previous studies.
PARKINSONISM & RELATED DISORDERS
(2022)
Article
Cell Biology
Daniel Rees, Amy L. Beynon, Mariah J. Lelos, Gaynor A. Smith, Luke D. Roberts, Lyndsey Phelps, Stephen B. Dunnett, Alwena H. Morgan, Rowan M. Brown, Timothy Wells, Jeffrey S. Davies
Summary: The feeding-related hormone, acyl-ghrelin, has a potential protective effect on dopamine neurons in experimental models of Parkinson's disease. However, its effect on dopaminergic neurons in the 6-OHDA rat medial forebrain bundle lesion model is unknown.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Genetics & Heredity
Sergey Lobanov, Branduff McAllister, Mia McDade-Kumar, G. Bernhard Landwehrmeyer, Michael Orth, Anne E. Rosser, Jane S. Paulsen, Jong-Min Lee, Marcy E. MacDonald, James F. Gusella, Jeffrey D. Long, Mina Ryten, Nigel Williams, Peter Holmans, Thomas H. Massey, Lesley Jones
Summary: This study found a significant association between age at onset of Huntington's disease and the length of a quasi-tandem repeat (QTR) in the TCERG1 gene, indicating that each added repeat hexamer can reduce age at onset by one year.
NPJ GENOMIC MEDICINE
(2022)
Article
Behavioral Sciences
Laurie Lemoine, Marine Lunven, Nicolas Fraisse, Katia Youssov, Blanche Bapst, Graca Morgado, Ralf Reilmann, Monica Busse, David Craufurd, Anne Rosser, Vincent de Gardelle, Anne-Catherine Bachoud-Levi
Summary: This study investigated whether patients with Huntington's disease and presymptomatic gene carriers had a deficit in time production tasks and found that HD patients exhibited a clear deficit in temporal production, while Pre-HD participants performed similarly to healthy controls. The ability of temporal production was associated with grey matter volume in the amygdala and caudate, and with clinical assessments of psychomotor and executive functions.
Article
Biochemistry & Molecular Biology
Francesca Keefe, Jimena Monzon-Sandoval, Anne E. Rosser, Caleb Webber, Meng Li
Summary: Inhibitory GABAergic interneurons originating in the embryonic medial ganglionic eminence (MGE) play a crucial role in controlling network activity in the neocortex. Dysfunctions in these cells are associated with seizure-based neurological disorders like epilepsy, autism, and schizophrenia. However, our understanding of the development of this diverse neuronal population is still incomplete. This study conducted single-cell RNA sequencing of human foetal MGEs and identified species-conserved transcriptomic profiles and potential transcription regulators for human interneuron differentiation, providing insights for in vitro modeling of interneuron development and potential therapeutic strategies using human pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
S. P. Clinch, M. Busse, J. Griffiths, A. E. Rosser, M. J. Lelos
Summary: The Stroop test is widely used in neuropsychology to assess attention and conflict resolution, and it is sensitive in detecting various diseases including Alzheimer's, Parkinson's and Huntington's diseases. The rodent analogue of the Stroop test, called the Response-Conflict task (rRCT), allows for systematic investigation of the neural systems involved in performing this test. This study aimed to examine the involvement of different subregions of the basal ganglia in conflict resolution processing by exposing rats to congruent or incongruent stimuli in the rRCT and analyzing the expression patterns of the immediate early gene Zif268 in cortical, hippocampal, and basal ganglia subregions. The results confirmed the involvement of prefrontal cortical and hippocampal regions, identified a specific role of the dysgranular retrosplenial cortex, and found a correlation between reduced neural activation in the dorsomedial striatum and performance accuracy. This study provides insights into the neuroanatomical changes underlying impaired Stroop performance in individuals with neurological disorders.
Article
Health Care Sciences & Services
Claudia Metzler-Baddeley, Monica Busse, Cheney Drew, Philip Pallmann, Jaime Cantera, Vasileios Ioakeimidis, Anne Rosser
Summary: This study used an integrated knowledge translation (IKT) framework to co-design a tablet-based rhythmic drumming training app (HD-DRUM) to stimulate cognitive and motor functions in people with Huntington disease (HD). Through surveying the accessibility needs of HD patients and conducting usability testing, key design elements were identified and implemented to create the HD-DRUM app. Evaluating the importance of this app could help improve cognitive and motor functions in HD patients.
JMIR FORMATIVE RESEARCH
(2023)
Article
Clinical Neurology
Duncan James McLauchlan, Thomas Lancaster, David Craufurd, David E. J. Linden, Anne E. Rosser
Summary: Depression is more common in neurodegenerative diseases like Huntington's disease, and the choice of antidepressant treatment for Huntington's disease lacks sufficient evidence. This study used propensity score analyses and cognitive battery assessments to determine the best treatment choices for depression in Huntington's disease and identified the main mechanisms underlying depression.
BRAIN COMMUNICATIONS
(2022)
Meeting Abstract
Clinical Neurology
Claudia Metzler-Baddeley, Monica E. Busse, Cheney Jg Drew, Philip Pallmann, Derek K. Jones, Anne E. Rosser
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2022)
Article
Neurosciences
Wenzhu Wang, Zihan Li, Yitong Yan, Shuo Wu, Xinyu Yao, Chen Gao, Lanxiang Liu, Yan Yu
Summary: This study investigated the reparative mechanisms of low-intensity pulsed ultrasound (LIPUS) in traumatic brain injury (TBI) and found that LIPUS promotes hippocampal neurogenesis, enhances neural electrical activity and neural plasticity, ultimately restoring neuronal function and cognitive capabilities in TBI mice.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Wenmin Yi, Fei Chen, Minghao Yuan, Chuanling Wang, Shengyuan Wang, Jie Wen, Qian Zou, Yinshuang Pu, Zhiyou Cai
Summary: The study suggests that a high-fat diet may lead to tau hyperphosphorylation and synaptic dysfunction by inhibiting the SIRT1/AMPK pathway and disrupting autophagy flux, ultimately resulting in cognitive decline.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Kim M. Hemsley, Helen Beard, Glyn Chidlow, Teresa Mammone, Leanne K. Winner, Daniel Neumann, Barbara King, Marten F. Snel, Paul J. Trim, Robert J. Casson
Summary: Optical coherence tomography (OCT) is a non-invasive method that can be used to rapidly and quantitatively examine the integrity of the neuroretina. It has been shown that OCT can be used to observe retinal thinning in patients with childhood dementia, and to assess the improvement of retinal structure after treatment. Furthermore, OCT can provide insights into other childhood dementias based on the correlation between retinal and brain degeneration in Sanfilippo syndrome.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Qianling Jiang, Xin Ma, Gaochen Zhu, Wen Si, Lingyu He, Guan Yang
Summary: This study investigated the effects of EAE induction on thymopoiesis and T cell development, revealing changes such as increased apoptosis, decreased proliferation, and a blockade in the transition from double-negative thymocytes to double-positive cells. It was also found that positive selection was disrupted in the thymus of EAE mice, along with an increased production of regulatory T cells.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Alice McDouall, Guido Wassink, Sumudu Ranasinghe, Kelly Q. Zhou, Rashika N. Karunasinghe, Justin M. Dean, Joanne O. Davidson
Summary: This study found that blocking connexin 43 hemichannels can attenuate brain injury and promote neurodevelopment in infants with mild hypoxic-ischemic encephalopathy, without causing hypothermia.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Hannah Sweetman, Mahmudur Rahman, Aditya Vedantam, Kajana Satkunendrarajah
Summary: Degenerative cervical myelopathy (DCM) is a neurological condition characterized by chronic compression of the cervical spinal cord, leading to impaired limb function. While respiratory dysfunction is not a common symptom of DCM, it can affect the ventilatory response to respiratory challenges. Surgical decompression improves sensorimotor function in DCM, but its impact on respiratory function is unclear. This study evaluates respiratory function and adaptive ventilation in a DCM model, showing that DCM impairs acute adaptive ventilatory ability and surgical decompression does not fully restore it.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Chengmei Sun, Muhammad Saif Ur Rahman, Budbazar Enkhjargal, Jianhua Peng, Keren Zhou, Zhiyi Xie, Lingyun Wu, Tongyu Zhang, Qiquan Zhu, Jiping Tang, Yujia Zeng, John H. Zhang, Shanshan Xu
Summary: This study found that Osteopontin (OPN) can attenuate inflammatory responses after subarachnoid hemorrhage (SAH) by promoting an anti-inflammatory microglial state. This effect may be mediated through the integrin-FAK-STAT3 and NF-kappa B signaling pathways.
EXPERIMENTAL NEUROLOGY
(2024)
Article
Neurosciences
Yang Yang, Xuezhu Chen, Chuanyan Yang, Mei Liu, Qianying Huang, Likun Yang, Yuhai Wang, Hua Feng, Zhongyang Gao, Tunan Chen
Summary: The study explores the effects of specific chemogenetic stimulation of intact corticospinal tract on functional recovery after stroke in mice. The findings demonstrate that combining chemogenetic activation with rehabilitation training leads to significant motor functional recovery by promoting axon sprouting and rewiring new functional circuits.
EXPERIMENTAL NEUROLOGY
(2024)